JPH0747108A - Urease inhibitor and deodorant - Google Patents

Abstract

PURPOSE:To prevent the generation of ammonia from urea with a strong urease inhibiting activity. CONSTITUTION:A urease inhibitor prepared by containing at least one selected from the group of NF-86I, NF-86II, NPF-86IA, NPF-86IB, NPF-86IIA and NPF-86IIB as effective component, a urease inhibitor containing a hydrophilic solvent extract of betel palm as effective component, and deodorant containing at least one selected from the group of NF-86I, NF-86II, NPF-86IA, NPF-861B, NPF-86IIA and NPF-86IIB as effective component, a deodorant containing a hydrophilic solvent extract of betel palm and a deodorant containing a coarsely crushed matter or a powder of betel palm as effective component.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to urease inhibitors and deodorants, and more particularly to a deodorant for preventing a bad odor generated from excrements such as human excrement such as humans, pet animals and livestock.

[0002]

2. Description of the Related Art Ammonia, methyl mercaptan, amines and the like are known as malodorous substances generated from excretions such as animal excrement. The odor of kennels, etc. is what is called excrement and so-called animal odor peculiar to animals. Ammonia is predominant in the composition, and sulfur compounds are not a problem. The odor of laboratory animal facilities is mainly odor of excrement and has a strong ammonia odor. Various deodorants have been conventionally proposed in order to reduce or prevent these malodors. Examples of the substance having a deodorizing action include activated carbon and silica gel having an adsorbing action, calcium hydroxide having a neutralizing action, citric acid, and sodium thiosulfate having a redox action and potassium permanganate. There is also a masking method using an aromatic substance. However, the conventional technique cannot always obtain a satisfactory effect.

[0003]

DISCLOSURE OF THE INVENTION An object of the present invention is to provide a urease inhibitor which effectively inhibits urease, an enzyme that decomposes urea into ammonia and carbon dioxide, and a deodorant having high safety and high efficacy. Is.

[0004]

[Means for Solving the Problems] The present inventors have paid particular attention to ammonia among the bad odors generated by excrement. Ammonia is produced in manure, especially by decomposition of urea contained in large amounts in urine. Urea accounts for the largest proportion of the terminal degradation products, and in humans, it is 2 in the daily urine with a normal diet.
5 to 30 g is excreted, reaching nearly 87% of the total urinary nitrogen content. This urea is decomposed by urease, an enzyme produced by enterobacteria, into ammonia. Urease is widely distributed in the intestinal bacteria and other various microorganisms as well as in the plant kingdom, but it is not present in higher animals.

The present inventors considered that the substance that inhibits the enzyme urease can suppress the generation of ammonia and thus exert the deodorizing effect. Therefore, when we searched for various plant extracts in consideration of safety and natural orientation,
Substances NF-86I and NF-8 extracted from Areca
6II, NPF-86IA, NPF-86IB, NPF-
It was found that 86IIA and NPF-86IIB have urease inhibitory activity, and completed the present invention. These substances have an action of inhibiting α-amylase (JP-A-63-185995) and an action of inhibiting 5'-nucleotidase (JP-A-63-307892).
However, it was not known to have urease inhibitory activity. The present inventors have also found that an extract of areca with hydrophilic solvent has urease inhibitory activity.

Therefore, the present invention relates to NF-86I and NF-8.
6II, NPF-86IA, NPF-86IB, NPF-
The urease inhibitor contains at least one selected from the group consisting of 86IIA and NPF-86IIB as an active ingredient. The present invention is also a urease inhibitor containing a hydrophilic solvent extract of areca nut as an active ingredient. The above substances NF-86I, NF-86II, NPF-8
6IA, NPF-86IB, NPF-86IIA and NP
F-86IIB has the following physicochemical properties.

NF-86I Form: Light yellowish brown powder Melting point: Clear melting point, no decomposition point Elemental analysis: Carbon 56.30% Hydrogen 4.61% Nitrogen 0.2% or less Ash content 0.3% or less Molecular weight: 1,000 to 10,000 (by dialysis tube) Infrared absorption spectrum: ν ^KBr _max cm ^-1 ; 3430, 2940, 1610, 15
20, 1440, 1370, 1280, 1110, 10
60, 800

Ultraviolet absorption spectrum: Water 1% λ _max nm (E1 cm) 279 (135.7) Hydrochloric acid 1% λ _max nm (E1 cm) 279 (134.5) Sodium hydroxide 1% λ _max nm (E1 cm) 290 Shoulder (291.2), 420 shoulders (96.4), 500
Shoulder (60.6), Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: ferric chloride reaction Positive ninhydrin reaction Negative p-anisidine-phthalic acid reaction Negative aniline-diphenylamine reaction Negative Dragendorff reaction Negative stability: stable in powder state

NF-86II Form: Light yellowish brown powder Melting point: Clear melting point, no decomposition point Elemental analysis: Carbon 56.64% Hydrogen 4.59% Nitrogen 0.2% or less Ash content 0.3% or less Molecular weight: Over 10,000 (by dialysis tube) Infrared absorption spectrum: ν ^KBr _max cm ^-1 ; 3400, 2940, 1610, 15
20, 1450, 1370, 1290, 1110, 10
60, 800, 500

Ultraviolet absorption spectrum: Water 1% λ _max nm (E1 cm) 280 (145.3) Hydrochloric acid 1% λ _max nm (E1 cm) 279 (142.1) Sodium hydroxide 1% λ _max nm (E1 cm) 290 Shoulder (306.1), 415 Shoulder (100.0), 505
Shoulder (61.2), Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: ferric chloride reaction Positive ninhydrin reaction Negative p-anisidine-phthalic acid reaction Negative aniline-diphenylamine reaction Negative Dragendorff reaction Negative stability: stable in powder state

NPF-86IA Form: Light yellowish brown powder Melting point: No clear melting point or decomposition point Elemental analysis: Carbon 54.82% Hydrogen 4.52% Oxygen 37.93% Nitrogen 0.2% or less Ash 0.1. 2% or less Molecular weight: 5,620 (by gel permeation chromatography using polyethylene glycol as standard) Infrared absorption spectrum: ν ^KBr _max cm ⁻¹ ; 3400, 2940, 1610, 15
20, 1440, 1380, 1280, 1260, 12
10, 1160, 1100, 1060, 820, 800

Ultraviolet absorption spectrum: Water λ _max nm 279 Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: ferric chloride reaction Positive ninhydrin reaction Negative p-anisidine-phthalic acid reaction Negative aniline-diphenylamine reaction Negative Dragendorff reaction Negative stability: stable in powder state

NPF-86IB Form: Light yellowish brown powder Melting point: Clear melting point, no decomposition point shown Elemental analysis: Carbon 57.09% Hydrogen 4.45% Oxygen 35.03% Nitrogen 0.2% or less Ash 0. 2% or less Molecular weight: 5,000 (by gel permeation chromatography using polyethylene glycol as standard) Infrared absorption spectrum: ν ^KBr _max cm ⁻¹ ; 3400, 2930, 1610, 15
20, 1440, 1360, 1280, 1250, 12
00, 1160, 1100, 1060, 800

Ultraviolet absorption spectrum: Water λ _max nm 279 Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: ferric chloride reaction Positive ninhydrin reaction Negative p-anisidine-phthalic acid reaction Negative aniline-diphenylamine reaction Negative Dragendorff reaction Negative stability: stable in powder state

NPF-86IIA Form: light yellowish brown powder Melting point: clear melting point, no decomposition point Elemental analysis: carbon 51.44% hydrogen 4.44% nitrogen 0.1% or less ash 0.2% or less molecular weight: 29,400 (by gel permeation chromatography using polyethylene glycol as standard) Infrared absorption spectrum: ν ^KBr _max cm ⁻¹ ; 3400, 2950, 1610, 15
20, 1440, 1370, 1280, 1250, 12
10, 1160, 1100, 1060, 820, 800

Ultraviolet absorption spectrum: Water λ _max nm 279 Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: ferric chloride reaction Positive ninhydrin reaction Negative p-anisidine-phthalic acid reaction Negative aniline-diphenylamine reaction Negative Dragendorff reaction Negative stability: stable in powder state

NPF-86IIB Form: Light yellowish brown powder Melting point: No clear melting point or decomposing point Elemental analysis: Carbon 52.46% Hydrogen 4.42% Nitrogen 0.1% or less Ash content 0.2% or less Molecular weight: 8,610 (by gel permeation chromatography using polyethylene glycol as standard) Infrared absorption spectrum: ν ^KBr _max cm ⁻¹ ; 3400, 2930, 1610, 15
20, 1440, 1370, 1280, 1250, 12
00, 1160, 1100, 1060, 800

Ultraviolet absorption spectrum: Water λ _max nm 279 Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: ferric chloride reaction Positive ninhydrin reaction Negative p-anisidine-phthalic acid reaction Negative aniline-diphenylamine reaction Negative Dragendorff reaction Negative stability: stable in powder state

By the substance having urease inhibitory activity as described above, the decomposition of urea into ammonia can be suppressed, that is, the generation of a bad odor derived from ammonia can be eliminated. Therefore, the present invention also provides NF-8.
6I, NF-86II, NPF-86IA, NPF-86
The present invention relates to a deodorant containing at least one selected from the group consisting of IB, NPF-86IIA and NPF-86IIB as an active ingredient. The present invention also relates to a deodorant containing a hydrophilic solvent extract of areca nut as an active ingredient.
The present inventors have also found that the coarsely crushed areca or powder itself exhibits a deodorizing effect. Therefore, the present invention also relates to a deodorant containing a coarsely ground product or powder of areca nut as an active ingredient. Areca is a seed without the pericarp of Areca catechu L (Areca catechu L) (Palmaceae), which is found in various parts of Southeast Asia, and is known as astringent, salivary stimulant, tapeworm control drug, etc. There is. Areca nut are available on the market and are also available as a coarsely crushed product or as a powder. Further, the betel nut can be appropriately crushed into various particle sizes.
The coarsely crushed betel nut powder or powder used as an active ingredient of the deodorant of the present invention may be any of the above,
It may be degreased.

Hereinafter, NF-86I, NF-86II, NP which are the active ingredients of the urease inhibitor and deodorant of the present invention.
The method for extracting F-86IA, NPF-86IB, NPF-86IIA and NPF-86IIB will be described in detail. (1) Raw Material The above-mentioned areca nut is used as a raw material, but it is preferable to use one which has been subjected to treatments such as drying, crushing and crushing so that it can be easily processed and extracted. In addition, it is convenient to use a commercially available crude drug form.

(2) Extraction NF-86I and NF-86II are phenolic substances and are characterized by 5'-nucleotidase inhibitory activity, α-amylase inhibitory activity and urease inhibitory activity. By centrifugation, filtration, or the like, isolation and purification can be performed by applying an appropriate purification means using these inhibitory activities as an index. These methods can be applied alone or in combination in any order, or can be applied repeatedly as needed. An example of the extraction method of NF-86I and NF-86II will be described below. (A) Using a degreasing solvent such as hexane or ether, degrease the raw material at room temperature or by heating. (B) Air-dry or vacuum-dry the degreased raw material to remove the degreasing solvent. (C) Next, methanol is added to the extraction raw material and the extraction treatment is performed according to a conventional method. The amount of the solvent used for the extraction is 10
About 100 to 1000 ml per 0 g is suitable. Usually, extraction is performed under boiling conditions, but the active ingredient can be obtained even if extraction is performed in a low temperature room at 4 ° C. (D) After concentration of the obtained extract to dryness, water is added to form a suspension. This is filtered with filter paper. The insoluble matter is further added with water, stirred well, filtered, and combined with the previous filtrate.

(E) An equal amount of a non-hydrophilic organic solvent such as ethyl acetate or chloroform is added to this aqueous solution to remove the organic solvent-soluble portion. (F) The water layer from which the non-hydrophilic organic solvent-soluble portion was removed was placed in a dialysis tube having a molecular weight cut off of 1,000 (Spectra / Pore 6; manufactured by Spectrum Medical Industry Co., Ltd.) and dialyzed against water to form an internal solution. Fraction into external solution. (G) The dialyzed internal solution fractionated with a dialysis tube having a molecular weight cut off of 1,000 was further put into a dialysis tube having a molecular weight cut off of 10,000 (Spectra / Pore 6; manufactured by Spectrum Medical Industry Co., Ltd.), and water was added. Dialyze and fractionate into internal and external solutions. (H) When fractionated in this way, the molecular weight is 1,000 to 10,000.
The desired inhibitory activity was observed in 0, 10000 or more fractional portions, and both kinds of active substances can be obtained as light brown powders by an operation such as freeze-drying. The effective substances fractionated to have molecular weights of 1,000 to 10,000 and 10,000 or more are designated as NF-86I and NF-86II, respectively.

(3) NPF-86IA, NPF-86I
Separation of B, NPF-86IIA and NPF-86IIB In this way, NF-86 fractionated with the dialysis tube
I and NF-86II can each be further divided into two fractions. That is, NF-86I is a fraction of molecular weight 5,620 (NPF-86IA) and a fraction of molecular weight 5,000 (N
PF-86IB), and NF-86II has a molecular weight of 29,
It is composed of 400 fractions (NPF-86IIA) and molecular weight fractions of 8,610 (NPF-86IIB). These four substances (NPF-86IA, NPF-86IB,
Separation and purification of NPF-86IIA and NPF-86IIB) can be carried out by various known methods, but it is preferably carried out using a high performance liquid chromatograph under the following conditions.

(A) Separation column As the separation column, one packed with a partition / adsorption type resin, an ion exchange resin, a gel filtration type separating agent or the like can be used. In addition, it is also preferable to additionally install a device for performing automatic injection and automatic fractionation. (B) Eluent As an eluent, various buffer solutions such as water, methanol, water, acetonitrile, dimethylformamide, acetic acid, butanol, hexane and the like can be used alone or in a mixture at an arbitrary ratio. . (C) Index As an index for detecting the active substance of the present invention, 28
Absorbance at a wavelength of 0 nm, 5'-nucleotidase inhibitory activity,
An α-amylase inhibitory activity and a urease inhibitory activity can be used. (D) Treatment method As a treatment method, an open column, medium pressure or high pressure method can be used. NPF-86IA, NPF-
86IB, NPF-86IIA and NPF-86IIB are
High-performance liquid chromatographs each show a single peak, which is consistent with the urease inhibitory activity.

The above extraction operation is the most suitable method for obtaining the desired urease inhibitory substance by removing the aroma and color peculiar to the raw material plant. In addition, since these effective substances are soluble in methanol and water, the above-mentioned extraction method starts from the raw material methanol extract, but in order to save the expensive organic solvent, a large amount of water is used first. Alternatively, the same operation may be performed after extraction with hot water. Also, as is clear from the UV absorption spectra of these active substances, the active substance is colored from yellowish brown to reddish brown when made alkaline, so it is also effective to perform the entire extraction process using a mineral acid or an organic acid under weak acidity. It is a simple extraction means. Extracts of areca with hydrophilic solvent also show urease inhibitory activity. Examples of the hydrophilic solvent include water, methanol and ethanol. The extraction operation may be performed by adding a solvent to the extraction raw material and treating it according to a conventional method. Usually, extraction is carried out under boiling, but it is also possible to perform extraction at a temperature of about 4 ° C.

The urease inhibitor of the present invention may contain, in addition to the active ingredient thereof, an additive that can be appropriately selected unless it deteriorates the active ingredient or is toxic. The urease inhibitor of the present invention can be used alone or as a compounding ingredient in a reagent or a drug such as a deodorant. Examples of the form of the deodorant of the present invention include granules, tablets, powders, solutions, and spray types. Granules, tablets, the above extract which is the active ingredient of the present invention,
It can be made into a deodorant by formulating into a powder, a solution, a spray type or the like. These dosage forms can be manufactured by a conventional method. When it is used as granules or powder, it can be used in various particle sizes depending on the application and workability. The particle size of the coarsely crushed betel nut powder or powder, which is the active ingredient of the deodorant of the present invention, can also be varied depending on the purpose of use and workability, and is not particularly limited. Generally, a smaller particle size gives a larger surface area, which is more preferable. For example, a particle size of about 8 to 100 mesh is preferable from the standpoint of workability in order to put it in a deodorant sheet. When used with larger particles, betel nut granules and powders are also recommended.
The particle size is preferably larger than that of the mesh. The deodorant of the present invention may be used in a single amount of 0.01 to 100% by weight as an active ingredient.

In the deodorant of the present invention, in addition to the above-mentioned active ingredients, bactericides, fragrances, activated carbon and silica gel having an adsorbing action, calcium hydroxide having a neutralizing action, which are conventionally used in deodorizing agents. , Citric acid, and one or more known deodorants such as sodium thiosulfate and potassium permanganate having a redox action can be blended at the same time. As a method of using the deodorant of the present invention, a place where an animal urinates and defecates, for example, sand, a sheet,
It may be sprayed or sprayed on straw or sawdust, or placed in a sheet. The amount used is not particularly limited because it is greatly influenced by individual differences among animals and the strength of urease activity and is highly safe. Preferably, the active ingredient is 0.1% by weight based on the amount of urine.
If the above exists, the effect is sufficiently exhibited.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples, Test Examples and Examples, but the present invention is not limited thereto.

[Preparation example]

I. Preparation of NF-86I and NF-86II a) 100 g of roughly crushed and dried areca nut was mixed with 30 parts of hexane.
After immersing in 0 ml and allowing it to stand at room temperature for 24 hours, hexane was removed by filtration. This operation was performed 3 times to degrease. (B) The defatted areca nut was air dried for 30 minutes. C) The dried betel nut was soaked in 300 ml of methanol and extracted under boiling for 3 hours. This operation was repeated 3 times to collect the extract. D) The obtained extract was concentrated at 25 ° C. by an evaporator and dried under vacuum (yield 6.86 g). Water 15
0 ml was added, and the mixture was stirred and filtered. Insoluble matter is 100 more water
After adding ml and stirring, the mixture was filtered and the filtrate was collected. (E) 250 ml of ethyl acetate was added to this aqueous solution for extraction. This operation was repeated 3 times to remove the ethyl acetate-soluble portion. 1.85 g of insoluble matter remains, and ethyl acetate extract 0.61
g, 4.01 g of a water extract was obtained.

F) The water extract from which the non-hydrophilic organic solvent-soluble portion has been removed is placed in a dialysis tube having a molecular weight cut off of 1,000 (Spectra / Pore 6; Spectra Medical Industry Co., Ltd.), and water is added at 4 ° C. It was dialyzed and fractionated into an inner solution and an outer solution. G) The dialyzed inner solution fractionated with a dialysis tube having a molecular weight cut off of 1,000 is further put into a dialysis tube having a molecular weight cut off of 10,000 (Spectra / Pore 6; manufactured by Spectra Medical Industry Co., Ltd.), and the mixture is washed with water 4 It was dialyzed at ℃ and fractionated into an inner solution and an outer solution. H) Molecular weight of 1,000 to 10,000 and molecular weight of 10,000
From the above fractionated portion, the molecular weight of 1,000 was obtained by freeze-drying.
˜10000 fraction (NF-86I) and molecular weight of 100,0
0.50 g and 0.75 g of 00 or more fractions (NF-86II) were obtained as light brown powders, respectively.

II. NPF-86IA, NPF-86I
B, Preparation of NPF-86IIA and NPF-86IIB From NF-86I and NF-86II, NPF-86I
A, NPF-86IB, NPF-86IIA and NPF-
86IIB was separated and produced by high performance liquid chromatography. The conditions are as follows. Separation column: Packed with adsorption / distribution type resin (Shodex R
S-pack, DE-613: Showa Denko KK) Eluent: Water: Methanol = 1: 9 Detector: Ultraviolet spectrophotometer (Nippon Bunko Kogyo KK) 2
80nm NF-86I, 500mg to NPF-86IA36.7m
g, NPF-86IB244.2 mg was obtained. Also NF-8
6II 250mg, NPF-86IIA68.8mg, NPF
There was obtained -86IIB68.0 mg.

III. Preparation of Areca Methanol Extract a) 100 g of roughly crushed and dried areca
After immersing in 0 ml and allowing it to stand at room temperature for 24 hours, hexane was removed by filtration. This operation was performed 3 times to degrease. (B) The defatted areca nut was air dried for 30 minutes. C) The dried betel nut was soaked in 300 ml of methanol and extracted under boiling for 3 hours. This operation was repeated 3 times to collect the extract. D) The obtained extract was concentrated at 25 ° C. by an evaporator and dried under vacuum (yield 6.86 g).

[0032]

[Test Example 1] Measurement of urease inhibitory activity 2.75 ml of water, 180 µl of 5M-urea, 70 µl of phenol red (0.3 mg / ml) and a sample were kept at 30 ° C in a cell of an absorptiometer. Next, urease was added, and the absorbance of phenol red by the produced ammonia (550
nm) was measured for 20 minutes. The inhibitory activity was calculated from the control without addition of the sample and the absorbance value 20 minutes after the sample was added, and the 50% inhibitory concentration (IC ₅₀ ) was determined. As urease derived from jack bean (Wako Pharmaceutical: 138units / mg), B
Derived from acillus Pasterii (SIGMA: 6000 units / mg)
2 types were used. The results are shown in Table 1.

[0033]

[Table 1] ───────────────────────────── Urease Specimen IC ₅₀ (μg / ml) ───────── ───────────────────── Lentil-derived methanol extract 54.7 NF-86I 200 NF-86II 24.9 NPF-86IA 200 NPF-86IB 200 NPF-86IIA 15.3 NPF-86IIB 28.4 ───────────────────────────── Bacillus methanol extract 45.8 Pasteurii NF-86I 42 .0 origin NF-86II 7.1 NPF-86IA 45.2 NPF-86IB 44.8 NPF-86IIA 5.7 NPF-86IIB 7.5 ────────────────── ─────────────

As is clear from the results shown in Table 1, the active substance of the present invention exhibits a strong urease inhibitory activity and can be expected to prevent the generation of ammonia from urea in feces and urine.

[0035]

[Test Example 2] Suppression of urinary ammonia generation Rat urine was collected using a metabolic gauge. Add 1 ml of this urine and 1 ml of each sample solution to an Erlenmeyer flask. After incubating this flask at 37 ° C for 3 hours, gas detector tube (Gastec Co., Ltd., direct reading type gas detector tube model 801, GASTEC)
The ammonia concentration was measured at. The results are shown in Table 2.

[0036]

[Table 2] ─────────────────────────────── Sample final addition concentration Ammonia deodorization rate after 3 hours (%) Concentration (ppm) (%) ─────────────────────────────── Reference 0 200 0 ───────── ──────────────────────── NF-86I 2.5 60 70 0.25 150 150 25 ────────────── ────────────────── NF-86II 2.5 50 75 0.25 135 33 33 ─────────────────── ──────────── Methanol 2.5 45 78 extract 0.25 115 43 ────────────────────────── ──────

[0037]

[Test Example 3] Nine male rats (body weight: about 400 g) were placed in a 3-gauge, and three rats each were placed in a breeding room (75 x 130 x
180 cm) were prepared in two rooms and kept at a room temperature of 25 ° C. under a 12-hour light / dark cycle (illumination of 8:00 to 20:00).
Put filter paper on the floor, using one room as a control,
45 g of a polymer absorber Diawet (manufactured by Mitsubishi Yuka) was evenly spread per cage. In the other room, as a test area, filter paper was laid on the floor, and areca extract (NF-
56 g of a diamond wet containing 86% of 86II) was spread per cage. Then, the concentrations of total amine and ammonia generated over time were measured with the same gas detector tube as in Test Example 2. The results are shown in Table 3 below. The unit is ppm.

[0038]

[Table 3] ──────────────────────────────────── Sample 1st day 2nd day 3rd day Day 5 ──────────────────────────────────── Total amine concentration 5 7 35 100 Diamond wet ── ───────────────────────── Ammonia concentration 2 5 13 20 ─────────────────── ───────────────── 20% NF-86II Total amine concentration 5 3 3 25 Diawet ────────────────── ───────── Ammonia concentration 0 0 0 7 ──────────────────────────────────── ─

As is clear from Table 3, the active ingredient of the present invention can prevent the generation of total amine and ammonia from manure.

[0040]

[Test Example 4] Using 9 rats, in the same breeding method as in Test Example 3, one room was covered with filter paper as a control, and the other room was used as a test zone with filter paper.
Betel nut powder (manufactured by Hisashi Morikawa Yakuhin, particle size of about 20-100)
50 g per cage was laid. Then, the concentrations of total amine and ammonia generated over time were measured with the same gas detector tube as in Test Example 2. The results are shown in Table 4 below. The unit is ppm.

[0041]

[Table 4] ──────────────────────────────────── Sample 1st day 2nd day 3rd day Day 4 Day 5 ──────────────────────────────────── Total amine concentration 5 12 40 130 160 Filter paper only ──────────────────────────── Ammonia concentration 2 2.5 15 30 65 ────────────── ─────────────────────── Total amine concentration 0 0 8 30 60 Areca powder ───────────────── ─────────── Ammonia concentration 0 0 2 6 20 ──────────────────────────────── ────

As is clear from Table 4, areca powder can suppress the generation of total amines and ammonia from manure.

[0043]

Example 1 A liquid deodorant having the following composition was produced. Polyoxyethylene hydrogenated castor oil 1.5% by weight ethanol 10.0 fragrance (peppermint, menthol) 2.0 glycerin 20.0 benzoic acid ester suitable amount pigment suitable amount NF-86I 10.0 purified water suitable amount 100.0

[0044]

Example 2 A powdery deodorant having the following composition was produced. Activated carbon 65.0% by weight Ferrous sulphate 10.0 Ferric sulphate 5.0 NF-86II 20.0 100.0 This mixture can be used in a non-woven sheet or the like.

[0045]

Example 3 A spray deodorant having the following composition was produced. 1,3-Butylene glycol 4.5% by weight Ethanol 55.0 NPF-86IIA 5.0 NPF-86IIB 5.0 Purified water 20.0 Fragrance 100.0 N ₂ Gas internal pressure 6.5 ± 0.5 kg / cm ² was added.

[0046]

Example 4 A powdery deodorant having the following composition was produced. This deodorant can be used, for example, by putting it in a non-woven sheet or the like.

Example 5 A powdery deodorant having the following composition was produced. Cat sand 80 wt% Betel nut crushed product 20 (particle size: larger than 8 mesh) 100

[0047]

Effect of the Invention NF-86I, NF-86II, NPF-
86IA, NPF-86IB, NPF-86IIA, NP
Hydrophilic solvent extracts of F-86IIB and areca nut have strong urease inhibitory activity and can prevent ammonia generation from urea, and granules and powders of areca also show a strong deodorizing effect. To do.

Claims (5)

[Claims] 1. NF-86 having the following physicochemical properties:
I, NF-86II, NPF-86IA, NPF-86I
B, a urease inhibitor containing at least one selected from the group consisting of NPF-86IIA and NPF-86IIB as an active ingredient. NF-86I Shape: Light yellowish brown powder Melting point: No clear melting point or decomposition point Elemental analysis: Carbon 56.30% Hydrogen 4.61% Nitrogen 0.2% or less Ash content 0.3% or less Molecular weight: 1,000 ~ 10000 (by dialysis tube) Infrared absorption spectrum: ν ^KBr _max cm ^-1 ; 3430, 2940, 1610, 15
20, 1440, 1370, 1280, 1110, 10
60,800 UV absorption spectrum: Water 1% λ _max nm (E1cm) 279 (135.7) Hydrochloric acid 1% λ _max nm (E1cm) 279 (134.5) Sodium hydroxide 1% λ _max nm (E1cm) 290 Shoulder (291.2), 420 shoulders (96.4), 500 shoulders (60.6), Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: Ferric chloride reaction Positive Ninhydrin reaction Negative p-Anisidine-phthalic acid reaction Negative Aniline-diphenylamine reaction Negative Dragendorff reaction Negative Stability: Stable in powder state NF-86II Shape: Light yellowish brown powder Melting point: clear No melting point or decomposition point Elemental analysis: Carbon 56.64% Hydrogen 4.59% Nitrogen 0.2% or less Ash content 0.3% or less Molecular weight: 10000 or more (by dialysis tube) Infrared absorption spectrum: ν ^KBr _max cm ^-1 ; 3400, 2940, 1610, 15
20, 1450, 1370, 1290, 1110, 10
60, 800, 500 Ultraviolet absorption spectrum: Water 1% λ _max nm (E1 cm) 280 (145.3) Hydrochloric acid 1% λ _max nm (E1 cm) 279 (142.1) Sodium hydroxide 1% λ _max nm (E1 cm) 290 shoulders (306.1), 415 shoulders (100.0), 505 shoulders (61.2), Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: Ferric chloride reaction Positive Ninhydrin reaction Negative p-Anisidine-phthalic acid reaction Negative Aniline-diphenylamine reaction Negative Dragendorff reaction Negative Stability: Stable in powder state NPF-86IA Shape: Light yellowish brown powder Melting point: clear No elemental analysis: carbon 54.82% hydrogen 4.52% oxygen 37.93% nitrogen 0.2% or less ash 0.2% or less molecular weight: 5,620 (polyethylene glycol as standard) Infrared absorption spectrum: ν ^KBr _max cm ⁻¹ ; 3400, 2940, 1610, 15
20, 1440, 1380, 1280, 1260, 12
10, 1160, 1100, 1060, 820, 800 Ultraviolet absorption spectrum: Water λ _max nm 279 Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: Ferric chloride reaction Positive Ninhydrin reaction Negative p-Anisidine-phthalic acid reaction Negative Aniline-diphenylamine reaction Negative Dragendorff reaction Negative Stability: Stable in powder form NPF-86IB Shape: Light yellowish brown powder Melting point: clear No elemental analysis: carbon 57.09% hydrogen 4.45% oxygen 35.03% nitrogen 0.2% or less ash 0.2% or less molecular weight: 5,000 (polyethylene glycol as standard) Infrared absorption spectrum: ν ^KBr _max cm ^-1 ; 3400, 2930, 1610, 15
20, 1440, 1360, 1280, 1250, 12
00, 1160, 1100, 1060, 800 Ultraviolet absorption spectrum: Water λ _max nm 279 Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: ferric chloride reaction Positive ninhydrin reaction Negative p-anisidine-phthalic acid reaction Negative aniline-diphenylamine reaction Negative Dragendorff reaction Negative Stability: Stable in powder state NPF-86IIA Shape: Light yellowish brown powder Melting point: clear No elemental analysis: carbon 51.44% hydrogen 4.44% nitrogen 0.1% or less ash 0.2% or less molecular weight: 29,400 (by gel permeation chromatography with polyethylene glycol as standard) ) Infrared absorption spectrum: ν ^KBr _max cm ^-1 ; 3400, 2950, 1610, 15
20, 1440, 1370, 1280, 1250, 12
10, 1160, 1100, 1060, 820, 800 Ultraviolet absorption spectrum: Water λ _max nm 279 Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: Ferric chloride reaction Positive Ninhydrin reaction Negative p-Anisidine-phthalic acid reaction Negative Aniline-diphenylamine reaction Negative Dragendorff reaction Negative Stability: Stable in powder state NPF-86IIB Shape: Light yellowish brown powder Melting point: clear No elemental analysis: carbon 52.46% hydrogen 4.42% nitrogen 0.1% or less ash 0.2% or less molecular weight: 8,610 (by gel permeation chromatography with polyethylene glycol as standard) ) Infrared absorption spectrum: ν ^KBr _max cm ^-1 ; 3400, 2930, 1610, 15
20, 1440, 1370, 1280, 1250, 12
00, 1160, 1100, 1060, 800 Ultraviolet absorption spectrum: Water λ _max nm 279 Solubility: Soluble in water, methanol and ethanol. Insoluble in hexane, ether, ethyl acetate, chloroform. Color reaction: ferric chloride reaction Positive ninhydrin reaction Negative p-anisidine-phthalic acid reaction Negative aniline-diphenylamine reaction Negative Dragendorff reaction Negative stability: stable in powder state 2. A urease inhibitor containing a hydrophilic solvent extract of areca nut as an active ingredient. 3. The NF-86I and NF-8 according to claim 1.
6II, NPF-86IA, NPF-86IB, NPF-
A deodorant containing at least one selected from the group consisting of 86IIA and NPF-86IIB as an active ingredient. 4. A deodorant containing a hydrophilic solvent extract of areca nut as an active ingredient. 5. A deodorant containing a coarsely crushed betel nut powder or powder as an active ingredient.