JPH0742224B2 - Method for producing bath salt containing enzyme - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to a method for producing an enzyme-containing bath agent.

(B) Conventional Technology Conventionally, as an enzyme of a bath agent containing an enzyme, there is one in which a proteolytic enzyme of plant origin such as papain is used alone. Such a bath agent is capable of degrading proteins adhering to the human body by the contained enzyme component and promoting cleaning of the human body.

(C) Problems to be Solved by the Invention However, such a bathing agent containing a proteolytic enzyme of plant origin alone still has problems in the following points.

A single enzyme alone can have a high protein decomposing ability only in a narrow pH range or temperature range, and the skin may not be sufficiently cleaned due to a difference in pH due to water quality or a difference in temperature during use.

Therefore, it is easy to become a hotbed of white bacterium and the like, and it cannot prevent skin diseases.

Further, when the remaining hot water in the bath is used as washing water, the activity of the enzyme is low because it is used at a relatively low temperature, and the enhancement of the detergency by the enzyme cannot be expected.

Furthermore, the stability of the enzyme itself is not good, and the degradation ability of the enzyme may be deteriorated due to aging of the enzyme, and the desired effect may not be exhibited.

Therefore, in order to make the enzyme act in a wide pH range and temperature range, it has been proposed to mix enzymes of different microbial origin and animal origin in the bath salt. It has also been proposed to blend a crude drug in order to stabilize such enzyme components and the like in the base of the bath salt. However, 2
When blending more than one type of enzyme, and containing a crude drug, although the stabilization of each enzyme is improved, it is difficult to say that the reduction of enzyme activity due to the reaction between different types of enzymes is sufficient. Moreover, since it is necessary to make the composition of the crude drug an essential component, in consideration of the decrease in enzyme activity during long-term storage when applied to a bath agent containing no crude drug,
It has the drawback of being substantially difficult.

An object of the present invention is to provide an enzyme-containing bath agent that can solve the problems of such conventional bath agents.

(D) Means for Solving the Problems In the present invention, in mixing an enzyme component consisting of an enzyme of microbial origin, an enzyme of animal origin, and an enzyme of plant origin into a bath salt base, The present invention relates to a method for producing an enzyme-containing bath salt, which comprises granulating at least two kinds of the above.

Here, the enzymes of microbial origin are Bacillus subtilis, Aspergillus,
An enzyme having a proteolytic action and the like obtained from actinomycetes and the like.

The enzyme of animal origin refers to an enzyme having a proteolytic action, a lipolytic action, a starch decomposing action, or the like, such as pancreatin extracted from animal organs, lysodium or lysodium chloride extracted from egg white.

Furthermore, enzymes of plant origin are papain, bromelain,
Ficin, or an enzyme obtained from soybean, malt or the like, which has a proteolytic action, a lipolytic action, a starch decomposing action and the like.

In the present invention, at least two or more of each of the above-mentioned enzymes are granulated to prevent a marked decrease in enzyme activity due to reaction between different types of enzymes in the bath agent base.

Various forms of the enzyme granulation method are possible,
Although there are extrusion granulation, tumbling granulation, briquetting, etc., from the viewpoint of enzyme granulation, the extrusion granulation is carried out in consideration of activity reduction prevention and formulation stability etc. in the granulation process. Method is preferred.

Mass production of granules in the extrusion granulation can be carried out by a wet granulation method which is carried out in the steps of powder → mixing → hydrogenation → granulation → drying → sizing → sieving.

The blending ratio when the granulated enzyme is mixed with the bathing agent base is preferably visually distinguishable from the raw materials other than the enzyme or the components. From this viewpoint, the blending amount of the granulated enzyme is 3% by weight. It is desirable to set it as above.

However, since the basic amount of the enzyme is the effective amount of enzyme action and the safe amount, the amount must be based on the active unit in the bath agent.

Further, the particle size of the granulated enzyme cannot be unconditionally determined because it is related to the dosage form of the raw material or component other than the enzyme, but for example, when the other raw material or component of the bath agent is 100 mesh, 10 mesh It is preferably about the same.

Further, all of the above-mentioned enzymes of microbial origin, animal origin and plant origin also have an anti-inflammatory action.

The base itself in the present invention is well known as a base for bathing agents, and may be used by appropriately combining components such as sodium chloride, sodium hydrogen carbonate, borax, sodium sulfate and sodium sesquicarbonate. is there.

Furthermore, crude drug powders such as Chinese cabbage, senkyu, spruce, and peony, and crude drug extracts such as psyllium extract can be blended, and by blending the crude drug powders and crude drug extracts, enzymes and pigments can be stabilized over time.

(E) Action and effect Since the enzyme of plant origin, the enzyme of microbial origin and the enzyme of animal origin are different in the bands showing the highest values in the activity rates related to pH and temperature, respectively, the cooperation of these causes The band showing the maximum value can be widened, and the high cleaning action by the enzyme can be maintained regardless of the difference in water quality and the difference in operating temperature.

In the production method of the present invention, at least two kinds of enzymes of the above-mentioned plant-derived enzyme, microbial-derived enzyme and animal-derived enzyme are granulated. Can be prevented, and the above-mentioned effects can be maintained for a long period of time.

(F) Example Hereinafter, the method for producing the bath salt containing an enzyme according to the present invention will be described in detail based on Comparative Examples and Examples.

[First Comparative Example] First, ASP protease (50,000 units / g) that is an enzyme of microbial origin, and pancreatin that is an enzyme of animal origin,
Papain (30,000 units / g), which is an enzyme of plant origin, and the bases, sodium chloride, borax, and sodium hydrogen carbonate, are stirred and mixed. On the other hand, propylene glycol, dye (blue No. 1), dye (yellow No. 202 (1)) are stirred.
Mix.

Then, the latter stirring mixture and the fragrance | flavor are added to the former stirring mixture, and it is set as the comparative product 1.

The mixing ratio is shown in Table 1.

[First Example] The bathing agent according to the present example is intended to stabilize the enzyme.
At least one of an enzyme of microbial origin, an enzyme of animal origin, and an enzyme of plant origin is granulated, and its composition is shown in Table 1 as invention products 1 to 4.

In Table 1 above, Comparative product 1 is an enzyme of microbial origin,
Inventive product 1 is a case where neither a plant-derived enzyme nor an animal-derived enzyme is granulated, and Invention 1 is a case where an animal-derived enzyme and a plant-derived enzyme are granulated. The case where the enzyme of plant origin and the enzyme of microbial origin are granulated, the invention product 3 is the case where the enzyme of microbial origin and the enzyme of animal origin are granulated, and the invention product 4 is
Is the case where both the enzyme of microbial origin, the enzyme of animal origin and the enzyme of plant origin are granulated.

Such bath agents (invention products 1 to 4) are also manufactured by the same steps as in the first comparative example.

In addition, the residual activity ratios of the enzymes contained in the comparative product 1 and the invention products 1 to 4 were measured at room temperature (20 ° C to
27 ° C.) and storage temperature 40 ° C. were investigated, and the results are shown in the graphs of FIGS. 1 and 2.

As is clear from FIG. 1, after 100 days of storage at room temperature, no bath-forming agent (comparative product 1) has been granulated with any enzyme of microbial origin, animal origin or plant origin.
Shows a residual activity rate of only 6%, whereas the enzyme of animal origin and the enzyme of plant enzyme are granulated (Invention 1), the enzyme of microbial origin and the enzyme of plant enzyme are granulated. The product (invention product 2), the enzyme of microbial origin and the enzyme of animal origin are granulated (invention product 3), respectively.
The residual activity rates of 62%, 44% and 29% are shown, and 94% is obtained by granulating all the contained enzymes (Invention product 4).
Shows the residual activity rate of.

As is clear from FIG. 2, after 40 days of storage, the bath agent (Comparative Product 1) which had not granulated any of the enzyme of microbial origin, enzyme of animal origin and enzyme of plant origin remained at 70 days after storage. Where the activity rate is zero, the enzyme of animal origin and the enzyme of plant enzyme are granulated (invention product 1),
Granules of microorganism-derived enzyme and plant-derived enzyme (Invention Product 2) and granules of microbial-derived enzyme and animal-origin enzyme (Invention Product 3) were measured after 100 days had passed. The residual activity rates of 37%, 19% and 0% are shown, and the granulation of all the contained enzymes (Invention product 4) shows the residual activity rate of 56%.

As described above, the product of the present invention can retain high enzyme activity at both room temperature and 40 ° C. by granulating at least two kinds of enzymes.

[Second Example and Second Comparative Example] The same enzyme stabilization test by granulation as in the first example was conducted.
Actinomycetes protease as an enzyme of microbial origin (50,000 units
/ g), pancreatin as an enzyme of animal origin and bromelain (30,000 units / g) as an enzyme of plant origin were mixed with a bath agent (invention products 2, 7 to 10).

The composition of components of such a bath agent is shown in Table 2 and
The test results are shown in FIGS.

The comparative product 2 in Table 2 is a comparative product in which neither enzyme was granulated.

As is clear from FIG. 3 and FIG. 4, this example also shows that at room temperature and 40 ° C. by granulating at least one enzyme of plant, microbial or animal origin.
It is shown that the high activity of the enzyme can be retained at any of the temperatures.

[Third Example and Third Comparative Example] Further, with respect to Comparative Product 1 and Invention Products 1 to 4, the amount of enzyme further contained was measured.

The tyrosin-fluorin method was used as the measuring method, and 1 μg of thyrozine equivalent amount of phosphorin per minute was defined as 1 protease unit.

The results are shown in Table 3.

As is clear from Table 3, only the total titer could be measured for Comparative Product 1 in which all enzyme components were not granulated, but what kind of enzyme and what amount of active amount were contained Could not be measured.

That is, it was impossible to separate and quantify the comparative product 1.

On the other hand, with regard to the invention products 1 to 4, it was possible to accurately separate and quantify the amount of the enzyme of microbial origin, animal origin and plant origin, respectively.

This makes it possible to clearly indicate that a plurality of enzymes are contained and how much their active amount is, and it is possible to give the user a sense of security.

[Fourth Example and Fourth Comparative Example] The amount of enzyme in the third example was also measured for Comparative Product 2 and Invention Products 5 to 8 in the second Example and the second Comparative Example, and the results were evaluated as the fourth result. Shown in the table.

Also in this example, it is possible to clearly show that the bathing agent according to the present invention contains a plurality of enzymes and how much their active amounts are, thus giving the user a sense of security. .

[Brief description of drawings]

FIG. 1 and FIG. 2 are graphs showing the residual activity rates of the bath salts at room temperature and 40 ° C. of the first example and the first comparative example,
FIG. 3 and FIG. 4 are graphs showing the residual activity ratios of the bath agents at room temperature and 40 ° C. of the second example and the second comparative example.

Claims (1)

[Claims] 1. An enzyme of microbial origin and an enzyme of animal origin,
A method for producing an enzyme-containing bath agent, which comprises granulating at least two or more of the enzyme components in mixing an enzyme component comprising an enzyme of plant origin with a bath agent base.