JPH074169B2 - Method for producing water-soluble milk protein - Google Patents
Method for producing water-soluble milk proteinInfo
- Publication number
- JPH074169B2 JPH074169B2 JP3398088A JP3398088A JPH074169B2 JP H074169 B2 JPH074169 B2 JP H074169B2 JP 3398088 A JP3398088 A JP 3398088A JP 3398088 A JP3398088 A JP 3398088A JP H074169 B2 JPH074169 B2 JP H074169B2
- Authority
- JP
- Japan
- Prior art keywords
- milk
- milk protein
- casein
- soluble
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Dairy Products (AREA)
Description
【発明の詳細な説明】 産業上の利用分野 本発明は、ミネラル補給素材及び栄養食品の素材として
利用される、水に可溶性であつてその水溶液が２価或は
３価の金属イオンにより再凝固する特性を有する水可溶
性乳タンパク質の製造方法に関する。DETAILED DESCRIPTION OF THE INVENTION Industrial Field of the Invention The present invention is used as a mineral supplement material and a material for nutritional food, and is soluble in water, and its aqueous solution is re-coagulated with divalent or trivalent metal ions. And a method for producing a water-soluble milk protein having the following properties.
従来技術 従来、レンネツト反応している乳タンパク質粉末として
は、レンネツト、カゼイン、粉末チーズが知られている
が、これらは水溶性でないため、食品素材としての利用
上制約があるという問題がある。なお、牛乳をpH4.6で
等電点沈澱させて得られる酸カゼインは、そのpHを挙げ
ることにより水に溶解するが、上記等電点沈澱の際のpH
の調整に用いた陽イオン（主としてNa+、K+のような１
価の金属イオン）と結合していて（ソーダカゼイン又は
ポタシウムカゼイン）これら金属の含有量が比較的多
く、加うるに、カゼイン臭が強いという利用上の問題が
ある。2. Description of the Related Art Conventionally, rennet, casein, and powdered cheese have been known as milk protein powders that have undergone a rennett reaction, but these are not water-soluble, so there is a problem in that they are restricted in use as food materials. Incidentally, acid casein obtained by isoelectric precipitation of milk at pH 4.6 is dissolved in water by increasing the pH, but the pH at the time of the isoelectric precipitation is
The cations used for the adjustment (mainly Na + , K +
(Sodacasein or Potassium Casein), which has a relatively high content, and has a strong casein odor.
発明が解決しようとする課題 本発明は、水に可溶性であつて、Naのような金属の含有
量が極めて少くてカゼイン臭もほとんどなく、かつ水溶
液が２価或いは３価の金属イオンにより凝固する特性を
有する水可溶性乳タンパク質を製造するための方法を提
供することを課題とする。DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention The present invention is soluble in water, has a very low content of metals such as Na and has almost no casein odor, and the aqueous solution is solidified by divalent or trivalent metal ions. It is an object to provide a method for producing a water-soluble milk protein having characteristics.
以下本発明を詳しく説明する。The present invention will be described in detail below.
発明の構成 本発明の特徴は、牛乳又は加工乳のpHを6.0以上に保ち
ながら、その乳中のカゼインと結合しているカルシウム
を高度に脱塩したもの、もしくは更に濃縮したものを乾
燥するか、もしくは凝乳酵素を添加してレンネツト反応
を行つた後に乾燥することにある。Structure of the invention The feature of the present invention is that while keeping the pH of cow's milk or processed milk at 6.0 or more, highly desalted calcium bound to casein in the milk, or further concentrated one is dried. Or, it is to dry after the rennett reaction is performed by adding the milk-clotting enzyme.
課題を解決するための手段 本発明においては、原料としての牛乳又は加工乳を、ア
ルカリ、例えばNaOH又はKOHによりそのpHを6.0以上に保
ちながら脱塩処理して乳中のカゼインと結合しているカ
ルシウムを高度に脱塩する。このカルシウムの脱塩によ
り、スターターの添加及び凝乳酵素の添加によるカゼイ
ン中のｋ−カゼインのパラ−ｋ−カゼイン化の後におい
ても、凝固によるカード形成が起らなくなる。Means for Solving the Problems In the present invention, milk or processed milk as a raw material is combined with casein in milk by desalting while keeping its pH at 6.0 or higher with an alkali, for example, NaOH or KOH. Highly desalting calcium. This desalting of calcium prevents curd formation due to coagulation even after the formation of para-k-casein of k-casein in casein by the addition of a starter and the addition of a milk-clotting enzyme.
したがつて、上記によりカルシウムを脱塩した後の原料
乳を乾燥することにより、水に可溶性な乳タンパク質粉
末が得られ、また、上記脱塩後レンネツト反応を行つた
ものを乾燥すると、同時に水可溶性のレンネツト乳タン
パク質粉末が得られる。Therefore, by drying the raw material milk after desalting the calcium as described above, a water-soluble milk protein powder is obtained, and when the rennet reaction after desalting is dried, water is simultaneously obtained. A soluble rennet milk protein powder is obtained.
本発明で上記脱塩処理を行うには、電気透析法を利用す
ることが好ましく、これにより、通常、乳中のカルシウ
ムを90％脱塩し得る。すなわち、このようなカルシウム
の脱塩により、乳中のカゼインと結合しているカルシウ
ムが高度に脱塩されるため、得られた脱塩乳を乾燥した
場合は勿論のこと、該脱塩乳をレンネツト反応させても
凝固カード化が起らないので、これを乾燥粉末化しても
水に可溶性となる。In order to carry out the desalting treatment in the present invention, it is preferable to use an electrodialysis method, and normally 90% of calcium in milk can be desalted. That is, since calcium bound to casein in milk is highly desalted by such desalting of calcium, it goes without saying that the desalted milk obtained is dried, Since coagulation curd does not occur even when the rennet reaction is performed, it becomes soluble in water even when it is dried and powdered.
上記脱塩乳の乾燥粉末化に際しては、該乳を濃縮しても
よく、その場合には限外濾過法を用いると効率的に行い
得る。なお、脱塩乳の乾燥は凍結乾燥又は噴霧乾燥等で
行うとよい。When the desalted milk is dried and powdered, the milk may be concentrated, and in that case, an ultrafiltration method can be efficiently used. The desalted milk is preferably dried by freeze drying or spray drying.
上記のようにして得られる乳タンパク質粉末は、約25％
程度の濃度でも水によく溶解する良好な水可溶性を示
す。About 25% of the milk protein powder obtained as described above
It shows good water solubility, which is well soluble in water even at a moderate concentration.
また、乳タンパク質粉末はその水溶液に２価もしくは３
価の金属イオンを添加すると、凝固して可塑性を有する
プラスチツクカードを形成する。例えば、レンネツト乳
タンパク質粉末の20％水溶液に、CaCl2やFeSO4の２価の
金属塩の水溶液を添加するとストレツチ性のあるプラス
チツクカードを形成し、FeCl3の３価の金属塩水溶液を
添加するとストレツチ性をあまり示さないものの、良好
なカードを形成する。しかし、１価の金属塩であるNaCl
やKClの水溶液を添加してもカードを形成しない。Also, milk protein powder is divalent or trivalent in its aqueous solution.
Upon addition of valent metal ions, it solidifies to form a plastic card with plasticity. For example, a 20% aqueous solution of Ren'netsuto milk protein powder, the addition of an aqueous solution of divalent metal salts of CaCl 2 and FeSO 4 to form a plastisol poke card with Sutoretsuchi resistance, the addition of trivalent metal salt solution of FeCl 3 It forms a good card, although it does not show much stretchability. However, the monovalent metal salt NaCl
Does not form a card even when added with an aqueous solution of KCl.
発明の効果 以上述べたように、本発明によると、水に可溶性である
とともに、その水溶液に２価もしくは３価の金属イオン
を添加すると良好なプラスチツクカードを形成し、更に
成分的にはソーダカゼイン及びポタシウムカゼインと比
較し、Na及びＫ含有量が少ないという特性を有する乳タ
ンパク質粉末を得ることができる。EFFECTS OF THE INVENTION As described above, according to the present invention, it is soluble in water, and when a divalent or trivalent metal ion is added to the aqueous solution, a good plastic curd is formed, and as a component, sodacasein It is possible to obtain a milk protein powder having a characteristic that the content of Na and K is low as compared with that of potassium casein.
したがつて、本発明により得られる乳タンパク質粉末の
水溶液に、上記２価及び３価の金属イオンを栄養上有用
なミネラルを添加すると、このミネラルが結合した乳タ
ンパク質から成るカードを形成させることができる。Therefore, when the above-mentioned divalent and trivalent metal ions are added to the nutritionally useful mineral to the aqueous solution of the milk protein powder obtained by the present invention, a curd composed of the milk protein to which the mineral is bound can be formed. it can.
すなわち、本発明による乳タンパク質粉末は、種々の健
康食品の素材として広範囲に利用し得るので、従来の乳
タンパク質粉末にみられない用途に供することができ
る。That is, since the milk protein powder according to the present invention can be widely used as a raw material for various health foods, it can be used for applications not found in conventional milk protein powders.
以下に実施例を示して本発明を具体的に説明する。な
お、実施例中の％は特記しない限り重量を示す。The present invention will be specifically described below with reference to examples. In the examples,% means weight unless otherwise specified.
実施例１ 原料乳として、全固形分8.83％、脂肪0.08％、タンパク
質2.99％、乳糖4.44％及び灰分0.75％（カルシウム含有
量124mg/100g）を含有する脱脂乳60kgを用い、この脱脂
乳を電気透析装置（徳山會達社製、TS-24型）により、
カルシウム含有量10.9mg/100gになるまで高度脱塩し、
この間脱脂乳のpHをNaOHを少量添加しながら、6.2に保
持した。Example 1 As the raw material milk, 60 kg of skim milk containing total solids 8.83%, fat 0.08%, protein 2.99%, lactose 4.44% and ash 0.75% (calcium content 124 mg / 100 g) was used, and this skim milk was electrically converted. With a dialysis machine (TS-24 type manufactured by Tokuyama Akita Co., Ltd.)
Highly desalted until the calcium content reaches 10.9 mg / 100 g,
During this period, the pH of skim milk was maintained at 6.2 while adding a small amount of NaOH.
このようにして高度脱塩した脱脂乳を65℃で30分間低温
殺菌した後、50℃まで冷却し、回分式限外濾過装置（DD
S社製、LAB-20型、0.36m2、GR61PP膜）で濃縮倍率4.5倍
まで濃縮して、脱塩濃縮乳13.3kgを得た。この脱塩濃縮
乳12kgを30℃に冷却した後、これに乳酸菌スターターと
して凍結BD−スターターカルチヤー0.01％を接種し、更
にレンイネツト（クリスチヤン−ハンセン社製）30ppm
を添加して30分間レンネツト反応を行つた。The skim milk thus highly desalted is pasteurized at 65 ° C for 30 minutes, then cooled to 50 ° C, and the batch type ultrafiltration device (DD
S, LAB-20 type, 0.36 m 2 , GR61PP membrane) was concentrated to a concentration ratio of 4.5 times to obtain 13.3 kg of desalted concentrated milk. After cooling 12 kg of this desalted concentrated milk to 30 ° C., 0.01% of frozen BD-starter culture as a lactic acid bacterium starter was inoculated to this, and further Rennett (manufactured by Kristian-Hansen) 30 ppm.
Was added and the rennet reaction was carried out for 30 minutes.
次いで、これを二等分して、一方は液体窒素で短時間凍
結して凍結乾燥を行い、他方は噴霧乾燥を行つて、レン
ネツト乳タンパク質粉末1.2kgを得た。また、レンネツ
ト添加後30分後に85℃で10分間の加熱を行つてレンネツ
トを失活させた粉末を併せて得た。Then, this was divided into two, one was briefly frozen in liquid nitrogen and freeze-dried, and the other was spray-dried to obtain 1.2 kg of rennet milk protein powder. Further, 30 minutes after the addition of the rennet, heating was performed at 85 ° C. for 10 minutes to obtain a powder in which the rennet was inactivated.
これらの粉末を水で溶解したところ、いずれも25％濃度
まで溶解性は良好であつた。When these powders were dissolved in water, the solubility was good up to a concentration of 25%.
次の実施例２及び３は、本発明による乳タンパク質粉末
の応用例を示したものである。The following Examples 2 and 3 show application examples of the milk protein powder according to the present invention.
実施例２ 実施例１で得たレンネツト乳タンパク質粉末の20％水溶
液に、それぞれCaCl2、FeSO4、NaCl、FeCl3の各水溶液
を添加したところ、NaClではカードを形成しなかつた
が、CaCl2、FeSO4ではストレツチ性のあるプラスチツク
カードを形成し、FeCl3ではストレツチ性はあまり示さ
ないものの、良好なカードを形成した。Example 2 When 20% aqueous solution of rennet milk protein powder obtained in Example 1 was added with respective aqueous solutions of CaCl 2 , FeSO 4 , NaCl, and FeCl 3 , CaCl 2 did not form a card with NaCl. , FeSO 4 formed a plastic card with stretchability, and FeCl 3 formed a good card, although it did not show much stretchability.
実施例３ 実施例１で得たレンネツト乳タンパク質粉末の20％水溶
液を注射器に入れ、CaCl2及び、FeSO4の各水溶液中に滴
下したところ、球状の凝固カードを形成した。したがつ
て、この粉体を用いてのカゼインカプセル製造の可能性
が考えられる。Example 3 A 20% aqueous solution of rennet milk protein powder obtained in Example 1 was placed in a syringe and dropped into each aqueous solution of CaCl 2 and FeSO 4 to form a spherical coagulated card. Therefore, the possibility of producing casein capsules using this powder is considered.
Claims (3)
ら、その乳中のカゼインと結合しているカルシウムを高
度に脱塩したもの、もしくは更に濃縮したものを乾燥す
るか、もしくは凝乳酵素を添加してレンネツト反応を行
つた後に乾燥することを特徴とする水可溶性乳タンパク
質の製造方法。1. A method in which the calcium bound to casein in the milk is highly desalted or further concentrated while the pH of the milk or the processed milk is kept at 6.0 or higher is dried, or the milk is curd. A method for producing a water-soluble milk protein, which comprises adding an enzyme to carry out a Rennett reaction and then drying.
囲第（１）項記載の製造方法。2. The production method according to claim 1, wherein the desalting is performed by an electrodialysis method.
囲第（１）項記載の製造方法。3. The production method according to claim 1, wherein the concentration is carried out by an ultrafiltration method.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3398088A JPH074169B2 (en) | 1988-02-18 | 1988-02-18 | Method for producing water-soluble milk protein |
| NZ227938A NZ227938A (en) | 1988-02-18 | 1989-02-10 | Process for the production of water-soluble milk protein from cow's milk |
| AU29900/89A AU618985B2 (en) | 1988-02-18 | 1989-02-13 | Water-soluble milk protein and process for producing same |
| DK073889A DK73889A (en) | 1988-02-18 | 1989-02-17 | WATER SOLUBLE MILK PROTEIN AND PROCEDURE FOR PRODUCING THE SAME |
| CA 591431 CA1336240C (en) | 1988-02-18 | 1989-02-17 | Water-soluble milk protein and process for producing same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3398088A JPH074169B2 (en) | 1988-02-18 | 1988-02-18 | Method for producing water-soluble milk protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01211453A JPH01211453A (en) | 1989-08-24 |
| JPH074169B2 true JPH074169B2 (en) | 1995-01-25 |
Family
ID=12401634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3398088A Expired - Lifetime JPH074169B2 (en) | 1988-02-18 | 1988-02-18 | Method for producing water-soluble milk protein |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPH074169B2 (en) |
| AU (1) | AU618985B2 (en) |
| CA (1) | CA1336240C (en) |
| DK (1) | DK73889A (en) |
| NZ (1) | NZ227938A (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4524522B2 (en) * | 2000-11-15 | 2010-08-18 | 栗田工業株式会社 | Treatment method for protein-containing wastewater |
| WO2006066332A1 (en) * | 2004-12-23 | 2006-06-29 | Murray Goulburn Co-Operative Co Limited | Method of manufacture of a modified milk powder suitable as a rennet casein extender or replacer |
| US10667538B2 (en) | 2007-11-07 | 2020-06-02 | Leprino Foods Company | Non-fat dry milk production processes for cheesemaking |
-
1988
- 1988-02-18 JP JP3398088A patent/JPH074169B2/en not_active Expired - Lifetime
-
1989
- 1989-02-10 NZ NZ227938A patent/NZ227938A/en unknown
- 1989-02-13 AU AU29900/89A patent/AU618985B2/en not_active Ceased
- 1989-02-17 DK DK073889A patent/DK73889A/en not_active Application Discontinuation
- 1989-02-17 CA CA 591431 patent/CA1336240C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2990089A (en) | 1989-08-24 |
| AU618985B2 (en) | 1992-01-16 |
| CA1336240C (en) | 1995-07-11 |
| JPH01211453A (en) | 1989-08-24 |
| NZ227938A (en) | 1990-06-26 |
| DK73889D0 (en) | 1989-02-17 |
| DK73889A (en) | 1989-08-19 |
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