JPH07308172A - Casein phosphopeptide-containing food or drink for person having low calcium absorption - Google Patents

Abstract

PURPOSE:To obtain the subject food or drink containing casein phosphopeptide and calcium, excellent in calcium absorption promoting effect and capable of carrying out improvement on absorption of calcium to persons lowered in a rate or an amount of calcium absorption. CONSTITUTION:This casein phosphopeptide-containing food or drink for persons having low calcium absorbing ability, e.g. fruit drink contains casein phosphopeptide at a weight ratio of >=0.30 times based on calcium and has calcium absorption promoting effect and is capable of carrying out improvement on absorption of calcium to persons lowered in a rate or an amount of calcium absorption. The food or drink is obtained by reacting a casein with trypsin, treating the product with a cation exchange resin to purify the product, blending the prepared casein phosphopeptide with a calcium component such as calcium lactate, sugar and citric acid, etc., dissolving the blend in a warm water heated to 45 deg.C, mixing the solution with concentrated apple juice, flavor, etc., diluting the mixed solution with water, packing the diluted solution in a can, etc., and subjecting the interior of can, etc., to heat sterilization.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to calcium absorption promotion by adding casein phosphopeptides (CPP), which are phosphopeptides obtained by hydrolyzing casein, to foods and drinks. The present invention relates to foods and drinks with excellent effects.

[0002]

2. Description of the Related Art Calcium plays an important role in the formation of human bones and teeth, cell proliferation, metabolism and the like. In recent years, Japan's diet has become richer, but the Ministry of Health and Welfare
According to the annual National Nutrition Survey, calcium alone does not meet the adult requirement of 600 mg.

On the other hand, milk has long been known for its high nutritional integrity, and has been regarded as the most excellent source of calcium among all foods, but the goodness of milk is simply included. Not only the amount of calcium present, but also excellent absorption. As one of the reasons why the absorption rate of calcium in milk and dairy products is better than other foods, it has been clarified that CPP produced in the digestion process of casein present in milk has a calcium absorption promoting effect. Ta [Hiroshi Naito; Sakaeishoku, 39, 433 (1986)].

Naito et al. Found that the CPP produced in vivo as described above also has the effect of promoting calcium absorption in CPP produced in vitro, and by adding this to food, it was converted into soluble calcium in vivo. Has invented a health food and drink that can be absorbed into the skin (Japanese Patent Publication No. 2-7616).

Since CPP has the ability to maintain calcium in a solubilized state (conversely, to prevent precipitation) even in the presence of phosphate in the neutral region, CPP is present in the lower small intestine where calcium is passively transported. It is believed that the presence of is promoted absorption of calcium. These findings confirm that the presence of CPP increases the concentration of soluble calcium in the digestive tract, and as a result promotes absorption of calcium, by in vitro or small intestine ligation loop test. In addition, a remarkable calcium absorption promoting effect has been confirmed by a calcium balance test of whole animals (whole animals) by rats [Li Ren Shu et al.
45, 333 (1992)].

[0006]

However, there is a great difference between individuals in the absorption rate of calcium in humans, and it has been reported that, for example, in middle-aged women, it is 7 to 68% [RP Heaney et al .; Th.
e American Journal of Clinical Nutrition, 43, FEBRU
ARY, 299 (1986)]. For this reason, it is desired to make some improvements, especially for humans whose calcium absorption rate and amount are reduced, but sufficient knowledge to find effective improvement measures has not been available so far. The current situation is that they have not been obtained.

[0007]

[Means for Solving the Problems] The present inventors have conducted extensive research to solve the above problems, and as a result, found that CPP was found in humans.
It was confirmed for the first time that the absorption of calcium is promoted by providing the above-mentioned drug, and the effective and necessary amount of CPP can be set, and the present invention has been completed based on this finding.

That is, the present invention relates to a casein phosphopeptide-containing food or drink containing casein phosphopeptide and calcium.

Casein phosphopeptide is a hydrolyzate of casein and is a phosphopeptide having a calcium-solubilizing activity.

It is known that there are two typical types of CPP. That is, those derived from α-casein (α-CPP) and those derived from β-casein (β-CP
P) and the structure and characteristic values are as shown in Table 1.

[0011]

[Table 1]

Abbreviations for amino acid residues in the above structure are IUPA
This is due to C, and all are L-forms except glycine. Ser ^P represents phosphoserine.

However, the CPP used in the present invention is not limited to the above, and any CPP having an activity capable of solubilizing calcium may be used.

The CPP in the present invention utilizes the affinity of the trivalent iron ion for the phosphoserine assembly portion in CPP to separate the unphosphorylated peptide by iron affinity chromatography, and then reverse phase high speed manufactured by Waters. Liquid chromatography system 10 μm Nucleosil
It is one that is detected by ultraviolet absorption at 215 nm using a C18 column. The main components of CPP quantified by this method are α _s2 -casein-derived peptides (1-32, peptides consisting of the 1st to 32nd amino acids of α _s2 -casein. The same applies hereinafter), and β-casein-derived peptides. Peptide (1
~ 28) and phosphorus-containing degradation products [Hirayama, T. et al .; Bios
ci. Biotech. Biochem., 56 (7), 1126 (1992)].

The CPP of the present invention includes a casein degradation product containing a CPP fraction obtained by allowing trypsin to act on casein (hereinafter abbreviated as crude CPP), and a casein degradation product further debittered (hereinafter , Abbreviated as bitterness CPP), or a casein decomposition product purified and recovered by adding calcium ion and a hydrophilic organic solvent (hereinafter, purified CP).
Abbreviated as P. ) Etc.

More specifically, as the raw material casein used for the CPP of the present invention, various acid casein, casein sodium, casein calcium and the like are the best, but unpurified milk, skim milk and the like can be used as raw materials. Can be used. According to this production method, this raw material casein is dissolved in water, and an enzyme preparation containing trypsin or trypsin is added. As the trypsin or trypsin-containing enzyme agent used in the present invention, a commercially available pancreatic enzyme (pancreatin) is sufficient, but a crystalline grade is preferable in terms of yield and the like.

CPP is produced by reacting casein as a raw material with the above enzyme. At this time, industrial enzyme reaction conditions are as follows: the casein concentration during the reaction is 30 (W / W)% or less, preferably 2 to 30 (W / W)%, and the reaction pH is 6.0 to
9.0, the reaction temperature is 15 to 60 ° C., preferably 20 to 50 ° C., and CPP is efficiently produced under these reaction conditions. The amount of enzyme used is 0.001 to 2% as crystalline trypsin relative to the weight of casein relative to the substrate, and the treatment time is 5 minutes to 100 hours, and the reaction proceeds sufficiently to produce CPP.

The CPP-containing solution obtained as described above is spray-dried as it is after deactivating the enzyme to give crude C
PP can be obtained. For debittering, granular activated carbon such as Pittsburgh activated carbon CAL (organo), granular Shirasagi KL (Takeda Pharmaceutical Co., Ltd.), Kuraray Coal GLC (Kuraray Chemical), or Amberlite IR-120.
A (organo), Dowex HCR-W2 (Muromachi Kagaku) and other cation exchange resins (Japanese Patent Laid-Open No. 59-159792) or debitalase (Novo) and other debittering enzymes, followed by heat sterilization Spray-dried and debittering C
PP can be obtained. CPP content in crude CPP is 10
.About.15%, especially about 12% (in terms of solid content, the same applies below), and the CPP content in the debittering CPP is about 10 to 15%, especially about 12%. In order to obtain purified CPP, calcium ions and a hydrophilic organic solvent are added and purified and recovered. For example, the CPP-containing solution is adjusted to pH 4.5 to 5.0 to remove unreacted casein and some impurities by precipitation, and calcium chloride and ethanol are added to the supernatant to recover the CPP fraction as a precipitate, which is heat-sterilized and spray-dried. Can be obtained. The amount of calcium chloride added may be about 0.01 to 10%, and ethanol is equivalent to the CPP fraction.
To the extent that 60% or more can be precipitated, eg 30-80% at the final concentration
The degree is enough. The CPP content in the purified CPP is about 80 to 90%, especially about 87%.

The effect of CPP thus obtained on promoting calcium absorption has been confirmed by the in vitro or small intestine ligation loop test or the rat whole animal calcium balance test as described above. However, there have been no reports on human calcium absorption test. The present inventor conducted a calcium absorption test in humans and confirmed the calcium absorption promoting effect of CPP for the first time, and was further able to determine the effective necessary amount of CPP. The test examples will be shown below.

Test Example 1 Purpose The calcium absorption promoting effect of CPP has been confirmed in rats and the like, but few reports have been made on humans. The absorption of calcium in humans varies greatly among individuals, and it is generally considered difficult for humans, who originally have a high absorption rate of calcium, to further increase absorption. On the other hand, some improvement is desired for humans whose calcium absorption rate and absorption are decreased, and if the calcium absorption promoting effect of CPP can be confirmed in these humans, its significance is extremely significant. . Therefore, this time, a calcium absorption test of CPP was carried out in humans.

Test Content 1) Subjects 35 postmenopausal women 2) Ca source CaCO ₃ 29 people CaHPO ₄ 6 people 3) Dose Ca 250 mg alone (placebo group) Ca 250 mg + purified CPP (Meiji Seika) 87.5 mg (C)
PP administration group) 4) Test method The Ca absorption rate was determined by the single isotope method.

In the administration test, subjects were assigned to a placebo group and a CPP administration group by a random number method, and the test was conducted at intervals of 4 weeks by crossover.

The test subject prohibited alcohol drinking for 24 hours, brought him to the hospital in the morning in a fasted state, and in the middle of the meal (two toasts, butter, coffee or tea), the CPP administration group was treated with Ca250 mg (containing 5 μCi ⁴⁵ Ca). Purified CPP87.5m
g, placebo group Ca 250 mg (containing 5 μCi ⁴⁵ Ca)
He had to eat starch and banned eating and drinking except water until blood sampling. Five hours after ingestion, blood was collected from the arm, radioactivity was measured, and the Ca absorption rate was calculated by the following formula.

FxAbs = 0.3537 × (SA5 ^0.92373 ) ×
(Ht ^0.52847 ) × (Wt ^0.37213 ) FxAbs: Absorption rate SA5: Specific activity of serum Ca for 5 hours (intake Ca1
Fractions per g) Ht: Height (m) Wt: Weight (kg)

Results Table 2 shows the average values and standard deviations of the Ca absorption rates of a total of 35 subjects after the administration of placebo and after administration of CPP. Although no difference was observed between the two treatment groups in all 35 subjects, 17 subjects with low Ca absorption rate after placebo administration were divided into two with the average value of Ca absorption rate after placebo administration (36.9%) as a boundary. When a paired t-test was performed on the difference between administration of placebo and administration of CPP, the Ca absorption rate during administration of CPP was significantly (p <0.05) higher than that during administration of placebo. The results are shown in Table 3. In addition, regarding the 18 subjects having a high Ca absorption rate, the calcium absorption promoting effect of CPP was not observed. The results are shown in Table 4.

[0026]

[Table 2]

[0027]

[Table 3]

[0028]

[Table 4]

As described above, it was confirmed that by ingesting the CPP of the present invention together with calcium, the absorption of calcium is promoted particularly in humans having a low calcium absorption rate. It is considered that the absorption was promoted as a result of the increase of calcium concentration in the digestive tract due to the presence of CPP. It can be said that the CPP of the present invention is an excellent means for improving the absorption of calcium, especially for humans whose absorption rate of calcium is reduced.

As for the ratio of calcium to CPP, in the case of purified CPP from the results of this test, the weight ratio to calcium is 0.35 times, but the water content of purified CPP is about 2% and the purity is about 87%. When converted to purity, it is 0.35 x 0.98 x 0.
87 = 0.3 times. That is, the required amount of pure CPP is 0.3 times or more the weight ratio to calcium. The preferred ratio is 0.3-
It is about 5 times, particularly preferably about 0.3 to 2 times.

The food and drink of the present invention include confectionery such as tablet confectionery and biscuits, and beverages such as soft drinks. When the CPP is added to these, since the substance is non-toxic to the human body, there is no limit to the addition ratio, but in the case of beverages in general, considering the characteristics or economic efficiency of each food and drink. In the case of purified CPP, about 0.01 to 5%, preferably about 0.01 to 1% is suitable. Also, in the case of food, 0.1 to 15 in the case of crude CPP or debittering CPP.
%, Preferably about 0.2 to 5%.

Crude CPP or debittering CPP is suitable for general foods such as confectioneries such as tablets and biscuits, and refined CPP is suitable for beverages and the like which also require transparency.

The subject of the food and drink of the present invention is a low calcium absorber, and for example, a person having a calcium absorption rate of 35% or less, particularly 30% or less when calcium carbonate is administered is suitable. It is also especially effective for female seniors. The intake of this food or drink to these persons is preferably about 100 to 500 mg / dose as a calcium amount.

[0034]

[Examples] Specific examples will be described below.
These do not limit the invention.

Example 1 100 g of sugar, 8 g of calcium lactate, 3 g of citric acid and 0.5 g of purified CPP were dissolved in 500 ml of hot water at 45 ° C., and then 10 g of concentrated apple juice and 1 g of flavor were mixed. Furthermore, 377 ml of water was added and mixed, and the mixture was filled in a can container and then heat sterilized to prepare a can-filled fruit juice beverage.

Example 2 Wheat flour 440 g, sugar 240 g, margarine 200 g, whole egg powder 70
g, milk 30g, calcium carbonate 6g, debittering CPP6.3
g, vanilla essence 2.5g, and water 130ml to make a dough, which is spread and then molded and roasted at 170 ℃ ~ 180 ℃,
I made a cookie.

[0037]

INDUSTRIAL APPLICABILITY By ingesting the CPP of the present invention together with calcium, the absorption of calcium is promoted especially for humans having a low calcium absorption rate. In addition, since a small addition amount is sufficient in view of the weight ratio to calcium when used, it is also a great advantage that it can be added to many foods and drinks. The food / beverage product of the present invention obtained as described above is an excellent means for improving the absorption of calcium, especially for humans whose absorption rate or absorption amount of calcium is decreased by eating this food / drink. Can be provided, and its significance is extremely large.

Claims (5)

[Claims] 1. A casein phosphopeptide-containing food or drink for a low calcium absorber, characterized by containing casein phosphopeptide and calcium. 2. A casein phosphopeptide-containing food or drink for a low calcium absorber according to claim 1, wherein the casein phosphopeptide is contained in an amount of 0.30 times or more in weight ratio to calcium. 3. The casein phosphopeptide-containing food or drink for a low calcium absorber according to claim 1, wherein the casein phosphopeptide is a casein degradation product containing a casein phosphopeptide fraction obtained by allowing trypsin to act on casein. 4. The casein phosphopeptide is a casein degradation product containing a casein phosphopeptide fraction obtained by allowing trypsin to act on casein, and is a casein degradation product further debittered. Food and drink containing casein phosphopeptide for calcium absorber 5. A casein phosphopeptide is a casein degradation product containing a casein phosphopeptide fraction obtained by allowing trypsin to act on casein, which is further purified by collecting calcium ions and a hydrophilic organic solvent. The casein phosphopeptide-containing food or drink for a low calcium absorber according to claim 1, which is a casein degradation product.