JPH0724571B2 - Aseptic chamber for microscope observation - Google Patents

Description

TECHNICAL FIELD The present invention relates to a sterile chamber for microscopic observation, and in particular,
The present invention relates to a sterile chamber for microscope observation used for aseptic observation of cells and the like in a solution by an optical microscope.

[Conventional technology]

Along with the progress of biotechnology, there is an increasing need for aseptic microscopic observation of a small amount of living cells such as cells into which genes have been introduced and cells have been fused.

A sterile chamber is required for this type of observation, but it can be used universally as a research-supporting experimental instrument and is not commercially available.

For this reason, researchers have made their own aseptic chambers for the above observations, but this is the current situation.

[Problems to be Solved by the Invention]

As a typical example of a self-made sterile chamber by researchers,
Acrylic resin plate as a base plate, which is adhered to the outer peripheral edge of the cover plate with silicone grease, a hole is made in the petri dish, and a cover glass is adhered to the petri dish with an adhesive or the like to close the hole, There is a cover glass located inside the dish that holds cells to be observed with agar etc., but in any case, it is difficult to manufacture perfect cells, and there is a risk of cell contamination. It is high, and the operability, in other words, the handleability is poor.

The present invention has been made in view of the above problems, and an object thereof is to provide a sterile chamber for microscope observation that has high sterility and is excellent in operability.

[Means for Solving the Problems] According to the present invention, the above-described object is to provide a base plate having a sample table on one surface, and a surface plate having a thickness larger than the height of the sample table. A rubber-like elastic sheet that is releasably adhered to and has an opening in a portion corresponding to the sample base, and a cover glass that is attached to the rubber-like elastic sheet to close the opening, and that is adhered to the base plate This is achieved by a microscopic observation sterile chamber characterized in that a cover glass of a rubber-like elastic sheet and a gap for filling an observation sample are formed in the sample table.

The rubber-like elastic sheet may be preferably made of silicone rubber.

[Action]

According to the above-mentioned structure, the rubber-like elastic sheet, due to its own properties, is kept in good contact with the base plate to maintain good sealability on the sample table, and has high sterility under microscope observation. Will be secured.

〔Example〕

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 and FIG. 2 show one embodiment of the aseptic chamber for microscope observation according to the present invention. The aseptic chamber for microscopic observation has a rectangular base plate 1 and a rubber-like elastic sheet 3 having the same shape.

The base plate 1 is made of plate glass capable of withstanding heat sterilization for sterilization, for example, physicochemical glass such as quartz glass or high silicate glass, and may be generally colorless and transparent. A small quadrangular sample stand 5 is provided on one surface of the base plate 1, that is, the upper surface 1a in the figure. The sample table 5 may be made of the same material as the base plate 1 and has a sample table upper surface 5a that is one step higher than the upper surface 1a of the base plate 1. The height of the sample table 5 may be about 0.9 mm, which may be formed integrally with the base plate 1 or may be composed of a small thin plate glass piece attached to the base plate 1 by a transparent adhesive or the like. May be.

The rubber-like elastic sheet 3 is made of a rubber material, preferably transparent silicone rubber, which is repeatedly releasably adhered to the flat upper surface 1a of the glass base plate 1 and can withstand heat sterilization at about 120 ° C. for sterilization. One edge portion is bonded to the base plate 1 with the adhesive 7.

The rubber-like elastic sheet 3 accommodates the entire sample base 5 in a portion corresponding to the sample base 5, and has a larger rectangular opening 9 than this.
Is provided. A thin transparent cover glass 11 is adhered to the upper surface of the rubber-like elastic sheet 3 with an adhesive so as to close the opening 9.

The rubber-like elastic sheet 3 has a thickness larger than the height of the sample table 5,
For example, the bottom surface 11a of the cover glass 11 of the rubber-like elastic sheet 3 and the upper surface 5 of the sample table 5 which are in close contact with the upper surface of the base plate 1 have a thickness of about 1 mm.
A relatively narrow gap 13 is formed between a and a.

When performing microscopic observation of cells and the like using a microscope sterilizing chamber having the above-mentioned configuration, first, the entire sterilizing chamber is autoclaved at a heating temperature of about 121 ° C. for 20 minutes, and this is completed. After that, in a clean bench or in a sterile room, the non-adhesive of the rubber-like elastic sheet 3, that is, the end portion indicated by reference numeral 3a in the figure is grasped by a hand and is peeled off from the upper surface 1a of the base plate 1 and released. In this state, a solution containing cells to be observed is dropped on the upper surface 5a of the sample table 5. Then, after this, the end portion 3a is brought close to the upper surface 1a of the base plate 1 and the rubber-like elastic sheet 3 is attached.
Is again brought into contact with the upper surface 1a of the base plate 1.

The rubber-like elastic sheet 3 comes into close contact with the upper surface 1a of the base plate 1 in an airtight manner due to its own properties, and shields the space defined by the opening 9 from the outside without requiring silicone grease or the like. It becomes a closed state. At this time, the upper surface 5a of the sample table 5 and the cover glass
A narrow gap 13 is formed between the lower bottom face 11a of the sample 11 and the solution on the upper face 5a of the sample table 5 comes into wet contact with the lower bottom face 11a of the cover glass 11 while being filled in the gap 13. . This allows the cells in the solution to be microscopically observed from the cover glass 11 side appropriately.

The lower bottom surface 11a of the cover glass 11 does not adhere to the upper surface 5a of the sample table 5, and the gap 13 is formed between the two as described above. Therefore, in observing fragile cells such as plant protoplasts under a microscope. However, the cells are not crushed, and good microscope observation can be performed.

〔The invention's effect〕

As can be understood from the above description, in the microscopic observation aseptic chamber according to the present invention, the rubber-like elastic sheet is, due to its own properties, air-tightly adhered to the base plate so that silicone grease or the like is used. Even if it is not, the tightness is maintained in the sample stage, and high sterility is ensured under the microscope observation. Also, the observation sample can be loaded into the sample stage with one hand of the rubber-like elastic sheet. It can be carried out quickly with good operability only by winding it up from the base plate and then returning it.

[Brief description of drawings]

FIG. 1 is a perspective view showing an embodiment of a sterile chamber for microscopic observation according to the present invention with a partial cross section, and FIG.
FIG. 1 is a longitudinal sectional view of a microscope and an aseptic chamber for observation according to the present invention shown in the figure. 1 …… Base plate 3 …… Rubber-like elastic sheet 5 …… Sample stand 9 …… Opening 11 …… Cover glass 13 …… Gap

Claims (2)

[Claims] 1. A base plate having a sample table on one surface, and a thickness larger than the height of the sample table, which is releasably adhered to the one surface of the base plate and has an opening at a portion corresponding to the sample table. A rubber-like elastic sheet having the same, and a cover glass attached to the rubber-like elastic sheet to close the opening, and the observation glass is filled in the cover glass of the rubber-like elastic sheet and the sample table that are in close contact with the base plate. Aseptic chamber for microscopic observation, characterized in that it is configured to form a gap. 2. The aseptic chamber for microscopic observation according to claim 1, wherein the rubber-like elastic sheet is made of silicone rubber.