JPH0463583A - Aseptic chamber for microscope observation - Google Patents

Abstract

PURPOSE:To comfortably keep sealing property at a sample stage without using silicone grease, etc., and ensure high aseptic property under observation in a microscope by hermetically adhering a rubbery elastic sheet to a base plate. CONSTITUTION:The subject microscope has a base plate 1 having a sample stage 5 on a face, a rubbery elastic sheet 3 adhering to a face of the base plate in releasable state and having an aperture 9 at a part corresponding to the sample stage and a cover glass 11 fitted to the sheet to close said opening and a gap to charge an observing sample is formed between the cover glass and the sample stage. The rubbery elastic sheet is adhered to the base plate to comfortably keep sealing property at a sample stage and ensure high aseptic property under observation in a microscope.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a sterile chamber for microscopic observation, and in particular,
The present invention relates to a sterile chamber for microscopic observation used for sterile observation of cells, etc. in a solution using an optical microscope.

[Conventional technology]

With advances in biotechnology, there is an increasing need to aseptically observe small amounts of living cells, such as cells that have undergone gene introduction or cell fusion, under a microscope.

This type of observation requires a sterile chamber, but there are no commercially available sterile chambers that can be used for general purpose research support experiments.

For this reason, researchers currently make their own sterile chambers for the above-mentioned observations.

[Problem to be solved by the invention]

Typical examples of self-made sterile chambers by researchers include:
An acrylic resin plate is used as a base plate, and the outer periphery of the cover plate is adhered with silicone grease.A hole is made in the petri dish, and a cover glass is glued to the petri dish with adhesive or the like so as to close the hole. In some cases, the cells to be observed are held using agar or the like on the bottom surface of a cover glass located inside a petri dish, but in either case, it is difficult to manufacture a perfect one, and there is a risk of bacterial contamination. However, it is difficult to operate, in other words, it is difficult to handle.

In view of the above-mentioned problems, the present invention aims to provide a sterile chamber for microscopic observation that is highly sterile and has excellent operability.

[Means for Solving the Problems] According to the present invention, the above objects include: a base plate having a sample stage on one surface; and a base plate having a thickness greater than the height of the sample stage; a rubber-like elastic sheet that is in close contact with the base plate in a peelable manner and has an opening in a portion corresponding to the sample stage; and a cover glass that is attached to the rubber-like elastic sheet to close the opening; This is achieved by a sterile chamber for microscopic observation, characterized in that a cover glass made of a rubber-like elastic sheet and the sample stage are configured to form a gap in which the observation sample is filled.

The rubber-like elastic sheet may preferably be made of silicone rubber.

C effect] According to the above-described configuration, the rubber-like elastic sheet, due to its own properties, is in close contact with the base plate to maintain good sealing on the sample stage, making it possible to maintain good airtightness under microscopic observation. This ensures high sterility.

〔Example〕

The invention will now be described in detail by way of example embodiments with reference to the accompanying drawings.

1 and 2 show one embodiment of a sterile chamber for microscopic observation according to the present invention. The sterile chamber for microscopic observation has a rectangular base plate 1 and a rubber-like elastic sheet 3 having the same shape.

The base plate 1 is made of plate glass that can withstand heat sterilization in a sterilization chamber, for example, a glass for scientific use such as quartz glass or high silicate glass, and may be colorless and transparent for boat fishing. A small rectangular sample stage 5 is provided on one surface of the base plate 1, that is, the upper surface +a in the figure. The sample stage 5 may be made of the same material as the base plate 1, and has a sample stage upper surface 5a that is one step higher than the upper surface 1a of the base plate 1. This sample stage 5
The height of the base plate 1 may be on the order of 0.9 mm, and may be formed integrally with the base plate 1, or may be composed of a small thin plate glass piece affixed to the base plate 1 with a transparent adhesive or the like. Good too.

The rubber-like elastic sheet 3 is made of a rubber material that adheres to the flat upper surface 1a of the glass base plate 1 so that it can be repeatedly peeled off, and that can withstand heat sterilization at about 120°C for sterilization.
It is preferably made of transparent silicone rubber, and one edge is bonded to the base plate l with an adhesive 7.

In the portion of the rubber-like elastic sheet 3 corresponding to the sample stage 5, there is a larger rectangular opening 9 that accommodates the entire sample stage 5.
is provided. A thin transparent cover glass 11 is adhered to the upper surface of the rubber-like elastic sheet 3 with an adhesive so as to close the opening 9.

The rubber-like elastic sheet 7 has a thickness greater than the height of the sample stage 5,
For example, it has a thickness of about 1 mm, and because of this, the bottom surface 11a of the cover glass 11 of the rubber-like elastic sheet 3 that is in close contact with the top surface of the base plate 1 and the sample stage 5
A relatively narrow gap 13 is formed between the upper surface 5a and the upper surface 5a.

When performing microscopic observation of cells, etc. using a sterile chamber for microscopic observation having the above-mentioned configuration, the entire sterile chamber is first autoclaved at a heating temperature of about 121°C for 20 minutes, and after this is completed. the non-adhesive side of the rubber-like elastic sheet 3 on a clean bench or in a sterile room;
That is, grasp the end indicated by the reference numeral 3a in the figure and release it from the upper surface 1a of the base plate l, and in this state, place the cells to be observed on the upper surface 5a of the sample stage 5. Add solution containing dropwise. After this, the end portion 3a is attached to the upper surface of the base plate 1! a and bring the rubber-like elastic sheet 3 into contact with the upper surface 1a of the base plate l again.

By coming into contact with the upper surface 1a of the base plate 1, the rubber-like elastic sheet 3 adheres airtightly to the upper surface 1a of the base plate 1 due to its own properties, and does not require silicone grease or the like.
The space formed by the opening 9 is sealed off from the outside. At this time, a narrow gap 13 is formed between the top surface 5a of the sample stage 5 and the bottom surface 11a of the cover glass 11, and the solution on the top surface 5a of the sample stage 5 fills the gap 13 in the cover glass 11. comes into wet contact with the lower bottom surface 11a of. Thereby, microscopic observation of cells in the solution can be performed accurately from the cover glass 11 side.

The lower bottom surface 11a of the cover glass 11 is the upper surface 5a of the sample stage 5.
The cells do not come into close contact with each other, and the gap 13 is formed between them as described above, so that even when microscopically observing fragile cells such as plant protoplasts, these cells will not be crushed and a good result will be obtained. Microscopic observations can now be performed.

〔Effect of the invention〕

As can be understood from the above explanation, in the sterile chamber for microscopic observation according to the present invention, the rubber-like elastic sheet, due to its own properties, is in close contact with the base plate, so that silicone grease or the like can be used. Even if it is not necessary, the sealing performance of the sample stand can be maintained well, ensuring high sterility under microscope observation. This can be done quickly and easily by simply rolling it up from the base plate and then returning it to its original position.

[Brief explanation of drawings]

FIG. 1 is a partially cross-sectional perspective view of one embodiment of a sterile chamber for microscopic observation according to the present invention, and FIG.
1 is a longitudinal sectional view of a microscope and a sterile chamber for observation according to the present invention shown in the figure; FIG. 1...Base plate 3...Rubber-like elastic sheet 5...Sample stand 9...Aperture 11...Cover glass 13...Engraving of gap diagram 1 Applicant: BCC Technology Co., Ltd. Agent Person Patent attorney Yusuke Hiraki Agent Patent attorney Sada Ishii Figure 2 a13 1a Procedural amendment (method) 7. Details of the amendment As shown in the attached sheet, 22nd day of lθ, 1990 2゜3゜4゜ Invention Name Relationship to the case involving a person who corrects a sterile chamber for microscopic observation Patent applicant Location 2-8-7 Shibuya, Shibuya-ku, Tokyo Name BCC Technology Co., Ltd. Representative Address 1-15 Toranomon, Minato-ku, Tokyo No. 7 5゜Date drawing of amendment order

Claims (1)

[Claims] 1. A base plate having a sample stage on one surface, and a portion having a thickness greater than the height of the sample stage, and a part that is releasably attached to the one surface of the base plate and corresponds to the sample stage. a rubber-like elastic sheet having an opening therein, and a cover glass attached to the rubber-like elastic sheet to close the opening, the cover glass of the rubber-like elastic sheet being in close contact with the base plate and the sample stage being observed A sterile chamber for microscopic observation, characterized in that it is configured to form a gap in which a sample is filled. 2. The sterile chamber for microscopic observation according to claim 1, wherein the rubber-like elastic sheet is made of silicone rubber.