JPH0723771A - Apparatus for determination of photosynthesis rate of photosynthetic microorganism - Google Patents
Apparatus for determination of photosynthesis rate of photosynthetic microorganismInfo
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- JPH0723771A JPH0723771A JP16552293A JP16552293A JPH0723771A JP H0723771 A JPH0723771 A JP H0723771A JP 16552293 A JP16552293 A JP 16552293A JP 16552293 A JP16552293 A JP 16552293A JP H0723771 A JPH0723771 A JP H0723771A
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- Prior art keywords
- light
- photosynthetic
- incubator
- outer container
- light source
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
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- Sustainable Development (AREA)
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- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、光合成速度測定装置に
係わり、より詳細には、液体媒質中に懸濁する光合成微
細生物に対して全方向から選択された光波長領域を有す
る光を均一に照射し、光合成微生物の酸素発生及び炭酸
ガス吸収の速度を連続的に測定し、光合成微生物の最大
光合速度に近い実測値及び光波長別の光合成速度を測定
する装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a photosynthetic rate measuring device, and more particularly to a photosynthetic microscopic organism suspended in a liquid medium, which is capable of uniformizing light having a light wavelength region selected from all directions. The present invention relates to an apparatus for continuously measuring the oxygen generation rate and carbon dioxide absorption rate of a photosynthetic microorganism, and measuring the measured value close to the maximum photosynthetic rate of the photosynthetic microorganism and the photosynthetic rate for each light wavelength.
【０００２】本発明の装置により、光合成微生物の光合
成速度を測定する光環境において、最適な光照射条件で
ある光強度やスペクトルを全方向から均一に光合成微生
物の各菌体に照射できることから、光合成微細生物の最
大光合成速度の測定や各種光合成バイオリアクターの供
給する光源装置の光強度やスペクトル条件設計改良に利
用できる。With the device of the present invention, in the light environment for measuring the photosynthetic rate of photosynthetic microorganisms, it is possible to uniformly irradiate each microbial cell of photosynthetic microorganisms with light intensity and spectrum, which are optimum light irradiation conditions, from all directions. It can be used to measure the maximum photosynthetic rate of microbes and to improve the design of light intensity and spectrum conditions of light source devices supplied by various photosynthetic bioreactors.
【０００３】[0003]
【従来の技術】従来、光合成微細生物の光合成速度を測
定する装置での培養器への光照射は、平面状の培養器で
は、片面、或いは、両面から光を照射したり、円柱状の
培養器では、外部から光照射して光照射環境を設定して
いた（例えば、宮地重遠、加幅栄、村田吉男編：光合成
研究法：共立出版（１９８１）参照）。2. Description of the Related Art Conventionally, light irradiation to an incubator in an apparatus for measuring the photosynthetic rate of a photosynthetic microorganism is performed by irradiating light from one side or both sides in a planar incubator, or a columnar culture. In the container, the light irradiation environment was set by irradiating light from the outside (see, for example, Shigeto Miyaji, Sakae Kaba, Yoshio Murata: Photosynthesis Research Method: Kyoritsu Shuppan (1981)).
【０００４】[0004]
【発明が解決しようとする課題】光合成微細生物の最大
光合成の能力を測定するには、全方向から均一に入射さ
れた光が最適な光環境と考えられる。しかし、上記した
従来の一般的な光合成微細生物の光合成速度を測定する
方法では、光の強度やスペクトル更には照射される部分
で不均一が生じ、又光波長域の選択がむずかしく、光を
培養器に照射する場合も、光の方向性や光強度が不均一
になる可能性が高く、光合成微細生物の最大光合成速度
を測定できなかった。本発明の目的は、光合成微生物の
光合成速度を測定するための従来の光照射方法が有して
いた前述の問題点を解消とするものである。In order to measure the maximum photosynthetic ability of photosynthetic microbes, the light uniformly incident from all directions is considered to be the optimum light environment. However, in the above-mentioned conventional method for measuring the photosynthetic rate of general photosynthetic microorganisms, inhomogeneity occurs in the intensity and spectrum of light as well as the irradiated portion, and the selection of the light wavelength region is difficult, and the light is cultured. Even when irradiating the vessel, the direction and intensity of light were likely to be non-uniform, and the maximum photosynthetic rate of photosynthetic microorganisms could not be measured. An object of the present invention is to eliminate the above-mentioned problems that the conventional light irradiation method for measuring the photosynthetic rate of a photosynthetic microorganism has.
【０００５】[0005]
【課題を解決するための手段】本発明は、前述の間題点
を解決すべくなされたものであり、（１）内側表面に光
を散乱させる金属或いは白色試料を塗布した外容器と、
該外容器の中心付近に配設された培養容器と、前記外容
器に光を照射する光源装置とから成り、前記培養器は、
炭酸ガス及び酸素溶存ガス濃度測定用のセンサーと、該
培養器内部において光合成微生物の菌体が懸濁している
培養用液体の温度を一定にする温度制御装置と、該培養
器内部の培養用液体に懸濁している光合成微生物の菌体
を均一に拡散する撹拌装置とを備え、前記光源装置は、
前記外容器の内側に光を導入する光ファイバー又は石英
棒を有し、該光ファイバー又は石英棒からの光を前記金
属或いは白色試料を塗布した外容器の内表面で均一に拡
散し、均一な光強度と分光分布を有する選択的な間接光
を前記培養器内の光合成微生物に全方向から照射し、光
合成微生物の酸素発生と炭酸ガス吸収を連続的に計測可
能としたこと、更には、（２）前記外容器内側に半透過
散乱板又は反射散乱板を有し、該半透過散乱板又は反射
散乱板に入射された光を透過又は散乱し、さらに、外容
器内側の前記金属或いは白色試料を塗布した全表面で均
一な光強度と分光分布を有する光に拡散するようにした
こと、更には、（３）前記光ファイバー又は石英棒に導
入される光が太陽光採光装置で集光された自然光、又
は、人工光源で集光された光であること、更には、
（４）前記光ファイバー又は石英棒に導入される光が人
工光源の光をフィルター、レンズ、ミラー等を用いて波
長域を選択した光であること、更には、（５）前記培養
器が透明の材料で二重構造に、かつ、球形に構成され、
該二重構造の外側の空間に培養用液体の温度を一定に保
つための透明液体を循環させ、かつ、内側空間内に該内
側空間内の培養用液体と該培養用液体中に懸濁している
光合成微生物の菌体を均一に拡散するためのマグネット
付き又は磁性体の撹拌子を有し、該撹拌子を該培養器の
外側から撹拌するようにしたことを特徴としたものであ
る。The present invention has been made to solve the above-mentioned problems, and (1) an outer container coated with a metal or a white sample that scatters light on the inner surface,
The culture container, which is disposed near the center of the outer container, and a light source device that irradiates the outer container with light, the incubator,
A sensor for measuring the concentration of carbon dioxide gas and oxygen dissolved gas, a temperature control device for keeping the temperature of the culture liquid in which the cells of the photosynthetic microorganism are suspended inside the incubator, and the culture liquid inside the incubator And a stirring device for uniformly diffusing the cells of the photosynthetic microorganisms suspended in the light source device,
An optical fiber or a quartz rod for introducing light is provided inside the outer container, and the light from the optical fiber or the quartz rod is uniformly diffused on the inner surface of the outer container coated with the metal or the white sample to obtain a uniform light intensity. And irradiating the photosynthetic microorganisms in the incubator with selective indirect light from all directions so that oxygen generation and carbon dioxide absorption of the photosynthetic microorganisms can be continuously measured, and further (2) Having a semi-transmissive scattering plate or a reflective scattering plate inside the outer container, transmitting or scattering the light incident on the semi-transmissive scattering plate or the reflective scattering plate, and further applying the metal or white sample inside the outer container. The light having a uniform light intensity and a spectral distribution is diffused over the entire surface, and further, (3) the natural light in which the light introduced into the optical fiber or the quartz rod is condensed by the sunlight collecting device, Or use an artificial light source to collect light It was a light, further,
(4) The light introduced into the optical fiber or the quartz rod is light in which the wavelength range is selected from the light of an artificial light source by using a filter, a lens, a mirror, and (5) the incubator is transparent. It is made of material in a double structure and spherical.
A transparent liquid for keeping the temperature of the culture liquid constant in the outer space of the double structure is circulated, and the culture liquid in the inner space and the culture liquid are suspended in the inner space. The present invention is characterized by having a stirrer with a magnet or a magnetic body for uniformly diffusing the cells of the photosynthetic microorganism present, and stirring the stirrer from the outside of the incubator.
【０００６】[0006]
【作用】液体媒質中に懸濁する光合成微細生物に対して
全方向から選択された光波長領域を有する光を均一に照
射し、光合成微生物の酸素発生及び炭酸ガス吸収の速度
を連続的に測定し、光合成微生物の最大光合速度に近い
実測値及び光波長別の光合成速度を測定する。[Function] Photosynthetic microbes suspended in a liquid medium are uniformly irradiated with light having a light wavelength region selected from all directions, and the rates of oxygen generation and carbon dioxide absorption of photosynthetic microorganisms are continuously measured. Then, the measured value close to the maximum photosynthetic rate of the photosynthetic microorganism and the photosynthetic rate for each light wavelength are measured.
【０００７】[0007]
【実施例】図１は、本発明の光合成微生物の光合成測定
装置の構成図を示し、図中、１は各種光源からの光を集
光させる光源ユニット、２はフィルター、３は光伝送体
で、これらによって光源装置を構成している。光源ユニ
ット１からの光はフィルター２によって波長が選択され
た後、光伝送体３に導入される。光伝送体３は光源ユニ
ット１により集光された光を外容器９に固定された散乱
板４に伝送するもので、この拡散板４により伝送体３を
通して入射された光を散乱させ、外容器９の内面に塗布
した散乱材に照射する。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS FIG. 1 is a block diagram of a photosynthesis measuring apparatus for photosynthetic microorganisms of the present invention, in which 1 is a light source unit for collecting light from various light sources, 2 is a filter, and 3 is an optical transmission body. The light source device is constituted by these. The wavelength of light from the light source unit 1 is selected by the filter 2 and then introduced into the optical transmission body 3. The light transmission body 3 transmits the light collected by the light source unit 1 to the scattering plate 4 fixed to the outer container 9. The diffusion plate 4 scatters the light incident through the transmission body 3 and the outer container The scattering material applied to the inner surface of 9 is irradiated.
【０００８】図２は、前記光源ユニット１、フィルター
２、光伝送体３の関係を示す図で、図２に示した例にお
いては、光源ユニット１は、電球等の光源１ａ、該光源
１ａからの光を平行光線にして反射する放物反射ミラー
１ｂ、該反射ミラー１ｂによって反射された平行光線の
通路中に配設され、該平行光線中の所望の波長の光を選
択して透過するフィルター２1、及び、該フィルター２1
を通した光を収束するためのレンズ２2等を具備し、こ
のレンズ２2の焦点近傍に光伝送体（例えば、光ファイ
バー、又は、石英棒）３の受光端を配設する。従って、
光源１ａからの光のうち、所望の波長の光を選択して、
光伝送体３内に導入する。FIG. 2 is a diagram showing the relationship between the light source unit 1, the filter 2 and the light transmission body 3. In the example shown in FIG. 2, the light source unit 1 is a light source 1a such as a light bulb, and the light source 1a Parabolic reflection mirror 1b for reflecting the light of the above as parallel rays, and a filter disposed in the path of the parallel rays reflected by the reflection mirror 1b and selectively transmitting the light of a desired wavelength in the parallel rays. 2 1 and the filter 2 1
A lens 2 2 for converging the light passing therethrough is provided, and a light receiving end of a light transmission body (for example, an optical fiber or a quartz rod) 3 is arranged near the focal point of the lens 2 2 . Therefore,
From the light from the light source 1a, select the light of the desired wavelength,
It is introduced into the optical transmitter 3.
【０００９】図３は、前記光源ユニット１、フィルター
２、光導体３の関係の他の実施例を示す図で、図中、図
２に示した実施例と同様の作用をする部分には、図２の
場合と同一の参照番号が付してある。而して、この実施
例においては、図２に示した放物反射ミラー１ｂに代っ
て、楕円反射ミラー１ｃを使用し、この楕円反射ミラー
１ｃの一方の焦点に光源１ａを、他方の焦点に光伝送体
３の受光端面を配設するようにして、図２に示した実施
例におけるレンズ２2を省略し得るようにしたものであ
る。FIG. 3 is a diagram showing another embodiment of the relationship between the light source unit 1, the filter 2 and the light guide 3. In the figure, portions having the same functions as those of the embodiment shown in FIG. The same reference numerals as in FIG. 2 are attached. Thus, in this embodiment, an elliptical reflection mirror 1c is used in place of the parabolic reflection mirror 1b shown in FIG. 2, and the light source 1a is provided at one focus of the elliptical reflection mirror 1c and the other focus is provided. The light receiving end face of the optical transmission body 3 is disposed in the optical transmission member 3 so that the lens 2 2 in the embodiment shown in FIG. 2 can be omitted.
【００１０】なお、以上には、光源として、電球等の人
工光源を用いる例を示した説明したが、周知のように、
太陽光をレンズ等によって集束するとともに、該レンズ
の焦点位置に光伝送体の受光端を配設するようにし、も
って、太陽光（自然光）を光伝送体３を通して外容器９
内に導入するようにしてもよいことは容易に理解できよ
う。Although an example in which an artificial light source such as a light bulb is used as the light source has been described above, as is well known,
The sunlight is focused by a lens or the like, and the light receiving end of the light transmission body is arranged at the focal position of the lens, so that the sunlight (natural light) passes through the light transmission body 3 and the outer container 9
It can be easily understood that it may be installed inside.
【００１１】５は二重構造の培養器、６は溶存酸素また
は炭酸ガス濃度測定用のセンサー、７は溶存ガス連続測
定器、８は二重構造の培養器の外側に流す一定温度の液
体が循環する恒温槽、１０は撹拌子、１１はこの撹拌子
１０の回転数を制御する撹拌用モータで、撹拌子１０に
よって培養器５内の光合成微生物の菌体の撹拌をする。Reference numeral 5 is a double-structured incubator, 6 is a sensor for measuring the concentration of dissolved oxygen or carbon dioxide gas, 7 is a continuous dissolved gas measuring device, and 8 is a liquid at a constant temperature flowing outside the double-structured incubator. A circulating constant temperature bath, 10 is a stirrer, and 11 is a stirring motor that controls the rotation speed of the stirrer 10. The stirrer 10 stirs the cells of the photosynthetic microorganism in the incubator 5.
【００１２】図４は、前記培養器５の詳細を説明するた
めの拡大図で、図４（ａ）は、縦断面図、図４（ｂ）は
平断面図を示す。この培養器５は、透明材料５ａ，５ｂ
で二重構造に、かつ、球形に構成されており、この二重
構造の外側の空間５1には、培養用液体の温度を一定に
保つための透明液体が前述の恒温槽８から供給され、循
環されている。５ｃ，５ｃは前記恒温槽８から恒温の液
体を循環供給するチューブ８ａ，８ａに接続するための
接続部（恒温液出入口）である。５2は前記二重構造の
内側の空間で、この内側の空間５2内には、試料注入口
５ｅを通して試料が供給され、該試料内の溶存ガスは、
溶存ガスセンサー挿入孔５ｄを通して該内側空間５2内
に挿入される溶存ガスセンサー６によって検出される。FIG. 4 is an enlarged view for explaining the details of the incubator 5, FIG. 4 (a) is a vertical sectional view, and FIG. 4 (b) is a plan sectional view. This incubator 5 is made of transparent materials 5a and 5b.
In in double structure, and is configured in a spherical shape, the space 5 1 outside this double structure, clear liquid for keeping the temperature of the culture liquid at a constant level is supplied from the constant temperature bath 8 described above Is being circulated. Reference numerals 5c, 5c are connection portions (constant temperature liquid inlet / outlet) for connecting to the tubes 8a, 8a for circulating and supplying constant temperature liquid from the constant temperature bath 8. 5 2 is an inner space of the double structure, and the sample is supplied into the inner space 5 2 through the sample injection port 5 e, and the dissolved gas in the sample is
It is detected by the dissolved gas sensor 6 inserted into the inner space 5 2 through the dissolved gas sensor insertion hole 5d.
【００１３】図５は、前記外部容器９の詳細を説明する
ための図で、図５（ａ）は、平断面図、図５（ｂ）は、
縦断面図で、外容器９の内面には、金属或いは白色試料
９ａが塗布されており、散乱板４により拡散・散乱され
た光は、更に、均一な光強度の分光分布を有する光に拡
散され、外容器９内全体に均質な光を照射するようにな
っている。また、１１ａは、前記撹拌用モータ１１に連
結された撹拌モータ１０に連結された撹拌棒で、該撹拌
棒１１ａの先端には永久磁石１１ｂが取り付けられてお
り、この永久磁石１１ｂの回転によって、培養器５内に
配設されている永久磁石又は磁性体１０が駆動され、培
養器５内の試料を撹拌する。FIG. 5 is a view for explaining the details of the outer container 9, FIG. 5 (a) is a plane sectional view, and FIG. 5 (b) is
In a longitudinal sectional view, the inner surface of the outer container 9 is coated with a metal or white sample 9a, and the light diffused and scattered by the scattering plate 4 is further diffused into light having a spectral distribution with uniform light intensity. Therefore, the entire inside of the outer container 9 is irradiated with uniform light. Further, 11a is a stirring rod connected to the stirring motor 10 connected to the stirring motor 11, a permanent magnet 11b is attached to the tip of the stirring rod 11a, and by the rotation of the permanent magnet 11b, The permanent magnet or magnetic body 10 arranged in the incubator 5 is driven to stir the sample in the incubator 5.
【００１４】上述のように、本発明においては、培養器
５内部の培養用液体中に懸濁している光合成微生物の菌
体を均一に拡散する撹拌装置を備え、外容器９の内側
に、ランプの点灯による光、又は、太陽光等の光を光フ
ァイバー又は石英棒３を通して導入し、導入された光を
散乱板４によって散乱して該外容器９内を照射し、前記
の金属或いは白色試料を塗布した外容器９の内表面でこ
の光を均一に拡散し、均一な光強度と分光分布を有する
選択的な間接光を前記培養器内の光合成微生物に全方向
から照射し、もって、光合成微生物の酸素発生と炭酸ガ
ス吸収が連続的に計測できるようになっている。As described above, in the present invention, the stirring device for uniformly diffusing the cells of the photosynthetic microorganism suspended in the culture liquid inside the incubator 5 is provided, and the lamp is provided inside the outer container 9. The light generated by turning on the light, or the light such as sunlight is introduced through the optical fiber or the quartz rod 3, and the introduced light is scattered by the scattering plate 4 to irradiate the inside of the outer container 9, and the metal or white sample This light is uniformly diffused on the coated inner surface of the outer container 9, and selective indirect light having a uniform light intensity and a spectral distribution is applied to the photosynthetic microorganisms in the incubator from all directions. Oxygen generation and carbon dioxide absorption of can be continuously measured.
【００１５】外容器９の内側に固定された半透過散乱
板、又は反射散乱板４により、入射光は透過又は散乱さ
れ、さらに、外容器内側の金属或いは白色試料を塗布し
た全表面で均一な光強度と分光分布を有する光に拡散さ
れる。入射光として、例えば、太陽光採光装置で集光さ
れた自然光、又は、人工光源で集光された光を使用する
場合は、外容器９の外側に配設されたこれら光源装置か
らの光が光伝送体である光ファイバー又は石英棒を通し
て外容器９内に伝送される。入射光が前記人工光源であ
る場合は、フィルター、レンズ、ミラー等、を用いて波
長域を選択して外容器９内に導入する。培養器５は、透
明の材料で二重構造、かつ、球面に形成され、外側に培
養用液体の温度を一定の保つための透明液体が循環し、
培養器内部の培養用液体と懸濁している光合成微生物の
菌体を均一に拡散するためのマグネット付き撹拌子を組
み込み、外部から一定回転数で撹拌できるようになって
いる。The incident light is transmitted or scattered by the semi-transmissive scattering plate or the reflection scattering plate 4 fixed to the inside of the outer container 9. Further, the entire surface of the outer container coated with the metal or white sample is uniform. It is diffused into light having a light intensity and a spectral distribution. As the incident light, for example, when natural light collected by a sunlight collecting device or light collected by an artificial light source is used, light from these light source devices arranged outside the outer container 9 is The light is transmitted into the outer container 9 through an optical fiber or a quartz rod which is a light transmitter. When the incident light is the artificial light source, the wavelength range is selected by using a filter, a lens, a mirror, etc. and introduced into the outer container 9. The incubator 5 is made of a transparent material and has a double structure and is formed into a spherical surface, and a transparent liquid for maintaining a constant temperature of the culture liquid circulates outside,
A stirrer with a magnet for evenly diffusing the cells of the photosynthetic microorganism in suspension with the culture liquid inside the incubator is incorporated so that the stirring can be performed from the outside at a constant rotation speed.
【００１６】図６は、光合成微生物の光合成測定装置の
実施例を示し、図６（ａ）は、平面図、図６（ｂ）は、
正面図で、図中、図１乃至図５において説明した実施例
と同様の作用をする部分には、図１乃至図５と同一の参
照番号を付してある。FIG. 6 shows an embodiment of a photosynthesis measuring apparatus for photosynthetic microorganisms. FIG. 6 (a) is a plan view and FIG. 6 (b) is a plan view.
In the front view, in the drawing, the same reference numerals as those in FIGS. 1 to 5 are attached to the portions having the same operations as those of the embodiment described in FIGS. 1 to 5.
【００１７】[0017]
【発明の効果】本発明によると、均一な光強度と分光分
布を有する選択的な間接光が、従来の光照射法法に比較
して、光強度培養器内の光合成微生物に全方向から照射
されるので、光合成微生物が光合成飽和に達する光強度
を低くすることができ、かつ、各光合成微生物の菌体の
重量、または、含有色素当たりの酸素発生と炭酸ガス吸
収濃度の高い培養を連続的に計測できる。According to the present invention, selective indirect light having a uniform light intensity and spectral distribution irradiates photosynthetic microorganisms in a light intensity incubator from all directions as compared with the conventional light irradiation method. Therefore, the light intensity at which the photosynthetic microorganism reaches the photosynthetic saturation can be lowered, and the weight of the cells of each photosynthetic microorganism or the oxygen generation per pigment contained and the high carbon dioxide absorption concentration are continuously cultured. Can be measured.
【図１】本発明の光合成微生物の光合成測定装置の構成
を示す図である。FIG. 1 is a diagram showing a configuration of a photosynthetic measuring apparatus for photosynthetic microorganisms of the present invention.
【図２】人工光源を集光した光を光ファイバーまたは石
英棒に伝送させる他の方法を模式的に示す図である。FIG. 2 is a diagram schematically showing another method of transmitting light collected by an artificial light source to an optical fiber or a quartz rod.
【図３】人工光源を集光した光を光ファイバーまたは石
英棒に伝送させる他の方法を模式的に示す図である。FIG. 3 is a diagram schematically showing another method of transmitting light collected by an artificial light source to an optical fiber or a quartz rod.
【図４】培養部の構造を模式的に示す拡大側断面図、及
び、縦断面を示す図である。FIG. 4 is an enlarged side sectional view schematically showing the structure of a culture section and a diagram showing a vertical section.
【図５】光ファイバーより伝送された光を拡散する方法
を模式的に示す外部容器の平断面図、及び培養器内の試
料を撹拌する方法を示す縦断面図である。FIG. 5 is a plan sectional view of an external container schematically showing a method of diffusing light transmitted from an optical fiber, and a vertical sectional view showing a method of stirring a sample in an incubator.
【図６】培養容器の計測センサー、撹拌、温度コントロ
ール部の模式的に示す平面図及び側面図である。6A and 6B are a plan view and a side view schematically showing a measurement sensor of a culture container, stirring, and a temperature control unit.
１…光源ユニット、２…フィルター、３…光伝送体、４
…拡散板、５…培養器、６…溶存ガスセンサー、７…溶
存ガス測定器、８…恒温水槽、９…外溶器、１０…磁石
又は磁性体、１１…撹拌用モータ、１１ａ…撹拌機、１
１ｂ…磁石。1 ... Light source unit, 2 ... Filter, 3 ... Optical transmission body, 4
... Diffusion plate, 5 ... Incubator, 6 ... Dissolved gas sensor, 7 ... Dissolved gas measuring instrument, 8 ... Constant temperature water bath, 9 ... External dissolver, 10 ... Magnet or magnetic body, 11 ... Stirring motor, 11a ... Stirrer 1
1b ... a magnet.
Claims (5)
色試料を塗布した外容器と、該外容器の中心付近に配設
された培養容器と、前記外容器に光を照射する光源装置
とから成り、前記培養器は、炭酸ガス及び酸素溶存ガス
濃度測定用のセンサーと、該培養器内部において光合成
微生物の菌体が懸濁している培養用液体の温度を一定に
する温度制御装置と、該培養器内部の培養用液体に懸濁
している光合成微生物の菌体を均一に拡散する撹拌装置
とを備え、前記光源装置は、前記外容器の内側に光を導
入する光ファイバー又は石英棒を有し、該光ファイバー
又は石英棒からの光を前記金属或いは白色試料を塗布し
た外容器の内表面で均一に拡散し、均一な光強度と分光
分布を有する選択的な間接光を前記培養器内の光合成微
生物に全方向から照射し、光合成微生物の酸素発生と炭
酸ガス吸収を連続的に計測可能としたことを特徴とする
光合成微細生物の光合成速度測定装置。1. An outer container having an inner surface coated with a metal or a white sample that scatters light, a culture container disposed near the center of the outer container, and a light source device for irradiating the outer container with light. The incubator comprises a sensor for measuring the concentration of carbon dioxide gas and oxygen-dissolved gas, a temperature control device for keeping the temperature of the culture liquid in which the cells of the photosynthetic microorganism are suspended inside the incubator constant, The light source device comprises an optical fiber or a quartz rod for introducing light into the outer container, and a stirring device for uniformly diffusing the cells of the photosynthetic microorganism suspended in the culture liquid inside the incubator. The light from the optical fiber or the quartz rod is uniformly diffused on the inner surface of the outer container coated with the metal or white sample, and selective indirect light having a uniform light intensity and spectral distribution is photosynthesized in the incubator. Illuminates microorganisms from all directions A photosynthetic rate measurement device for photosynthetic microbes, which is capable of continuously measuring oxygen generation and carbon dioxide absorption of photosynthetic microorganisms.
散乱板を有し、該半透過散乱板又は反射散乱板に入射さ
れた光を透過又は散乱し、さらに、外容器内側の前記金
属或いは白色試料を塗布した全表面で均一な光強度と分
光分布を有する光に拡散するようにしたことを特徴とす
る請求項１に記載の光合成微細生物の光合成速度測定装
置。2. The metal inside the outer container has a semi-transmissive scattering plate or a reflective scattering plate inside, and transmits or scatters light incident on the semi-transmissive scattering plate or the reflective scattering plate. Alternatively, the photosynthetic rate measuring device for a photosynthetic microorganism according to claim 1, characterized in that light having a uniform light intensity and a spectral distribution is diffused over the entire surface coated with the white sample.
る光が太陽光採光装置で集光された自然光、又は、人工
光源で集光された光であることを特徴とする請求項１に
記載の光合成微細生物の光合成速度測定装置。3. The light introduced into the optical fiber or the quartz rod is natural light collected by a sunlight collecting device or light collected by an artificial light source. Photosynthetic microbiological photosynthesis rate measuring device.
る光が人工光源の光をフィルター、レンズ、ミラー等を
用いて波長域を選択した光であることを特徴とする請求
項１に記載の光合成微細生物の光合成速度測定装置。4. The photosynthesis according to claim 1, wherein the light introduced into the optical fiber or the quartz rod is light of a wavelength range selected from light of an artificial light source by using a filter, a lens, a mirror or the like. Photosynthetic rate measuring device for microscopic organisms.
かつ、球形に構成され、該二重構造の外側の空間に培養
用液体の温度を一定に保つための透明液体を循環させ、
かつ、内側空間内に該内側空間内の培養用液体と該培養
用液体中に懸濁している光合成微生物の菌体を均一に拡
散するためのマグネット付き又は磁性体の撹拌子を有
し、該撹拌子を該培養器の外側から撹拌するようにした
ことを特徴とする請求項１に記載の光合成微細生物の光
合成速度測定装置。5. The incubator has a double structure made of a transparent material,
And, it is formed in a spherical shape, and circulates a transparent liquid for keeping the temperature of the culture liquid constant in the space outside the double structure,
In addition, the inner space has a culture liquid in the inner space and a stirrer with a magnet or a magnetic body for uniformly diffusing cells of photosynthetic microorganisms suspended in the culture liquid, The photosynthetic rate measuring device for photosynthetic microbes according to claim 1, wherein the stirrer is stirred from the outside of the incubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5165522A JP3029952B2 (en) | 1993-07-05 | 1993-07-05 | Photosynthetic rate measurement device for photosynthetic micro organisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5165522A JP3029952B2 (en) | 1993-07-05 | 1993-07-05 | Photosynthetic rate measurement device for photosynthetic micro organisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0723771A true JPH0723771A (en) | 1995-01-27 |
| JP3029952B2 JP3029952B2 (en) | 2000-04-10 |
Family
ID=15813993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5165522A Expired - Fee Related JP3029952B2 (en) | 1993-07-05 | 1993-07-05 | Photosynthetic rate measurement device for photosynthetic micro organisms |
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| Country | Link |
|---|---|
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002092755A1 (en) * | 2001-04-12 | 2002-11-21 | Bio2 Lab Co., Ltd. | Apparatus for oxygen generation and carbon dioxide removal using photosynthetic microorganisms |
| KR100421823B1 (en) * | 2001-04-12 | 2004-03-10 | 김명운 | Apparatus for Oxygen generation and carbon dioxide removal using photosynthetic microorganisms |
| US9518248B2 (en) | 2010-11-15 | 2016-12-13 | Cornell University | Optofluidic photobioreactor apparatus, method, and applications |
| JP2017079602A (en) * | 2015-10-23 | 2017-05-18 | 株式会社デンソー | Culture apparatus and culture method of photosynthetic microorganism |
| CN112920952A (en) * | 2021-03-25 | 2021-06-08 | 广东轻工职业技术学院 | Photosynthetic organism culture apparatus |
-
1993
- 1993-07-05 JP JP5165522A patent/JP3029952B2/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002092755A1 (en) * | 2001-04-12 | 2002-11-21 | Bio2 Lab Co., Ltd. | Apparatus for oxygen generation and carbon dioxide removal using photosynthetic microorganisms |
| KR100421823B1 (en) * | 2001-04-12 | 2004-03-10 | 김명운 | Apparatus for Oxygen generation and carbon dioxide removal using photosynthetic microorganisms |
| US9518248B2 (en) | 2010-11-15 | 2016-12-13 | Cornell University | Optofluidic photobioreactor apparatus, method, and applications |
| US10604733B2 (en) | 2010-11-15 | 2020-03-31 | Cornell University | Optofluidic photobioreactor apparatus, method, and applications |
| US11186812B2 (en) | 2010-11-15 | 2021-11-30 | Cornell University | Optofluidic photobioreactor apparatus, method, and applications |
| JP2017079602A (en) * | 2015-10-23 | 2017-05-18 | 株式会社デンソー | Culture apparatus and culture method of photosynthetic microorganism |
| CN112920952A (en) * | 2021-03-25 | 2021-06-08 | 广东轻工职业技术学院 | Photosynthetic organism culture apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3029952B2 (en) | 2000-04-10 |
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