CN2475023Y - Light intensity attenuation determination instrument - Google Patents

Light intensity attenuation determination instrument Download PDF

Info

Publication number
CN2475023Y
CN2475023Y CN 01207695 CN01207695U CN2475023Y CN 2475023 Y CN2475023 Y CN 2475023Y CN 01207695 CN01207695 CN 01207695 CN 01207695 U CN01207695 U CN 01207695U CN 2475023 Y CN2475023 Y CN 2475023Y
Authority
CN
China
Prior art keywords
light
light source
groove
light intensity
hole
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 01207695
Other languages
Chinese (zh)
Inventor
曾文炉
李浩然
石红
丛威
蔡昭铃
欧阳藩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Process Engineering of CAS
Original Assignee
Institute of Chemical Metallurgy CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Chemical Metallurgy CAS filed Critical Institute of Chemical Metallurgy CAS
Priority to CN 01207695 priority Critical patent/CN2475023Y/en
Application granted granted Critical
Publication of CN2475023Y publication Critical patent/CN2475023Y/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Landscapes

  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)

Abstract

The utility model relates to a light intensity attenuation measuring device, comprising a light source part, an attenuating groove, an emplacing groove and a light intensity tester. The light source is arranged in an air-tight light source groove, a dimmer adjusts light intensity of the light source, and the front end face of the light source is provided with a light hole. The square emplacing groove clings to the front end of the light hole of the light source groove, with the front end of the square emplacing groove provided with a light hole corresponding to the light hole at the front end face of the light source groove. The square attenuating groove is arranged in the emplacing groove, the front and rear two translucent faces are made of optical glass, and the optic probe of the light intensity tester is arranged in the light hole at the front end of the emplacing groove. The light intensity attenuation measuring device of the utility model has the characteristics of adjustable light intensity, simple and rapid operation, convenient cleaning, precise result, great reproduction quality and the like.

Description

The light intensity attenuation determinator
The utility model relates to a kind of light intensity attenuation determinator of reaction system light intensity attenuation situation when being used to measure and study algae or other photosynthetic organism cell suspension cultures.
When algae and other photosynthetic organism cell suspension cultures, be to understand the demand of cell growth to luminous energy, what usually need to measure cultivating system is subjected to light and light intensity attenuation situation.Up to now, at specific cultivation requirement, the researchist has developed some light intensity attenuation assay method and technology, as the experimental formula method, and simple and easy beaker method.The experimental formula method is because it is the expression formula of a certain particular types photosynthetic cells resulting empirical under specific condition of culture, and universality is relatively poor; Though it is simple and easy that the beaker method is operated, result's accuracy and reappearance are not fully up to expectations.
The purpose of this utility model is to overcome many defectives and the deficiency that existing light intensity attenuation assay method and determinator exist, provide a kind of cheap, easy and simple to handle, accuracy of measurement is higher is applicable to the small light overdamp determinator that is subjected to light and light intensity attenuation situation of measuring little algae or other photosynthetic organism cell culture system.
Embodiment of the present utility model is as follows:
The light intensity attenuation determinator that the utility model provides comprises the Lights section 1, decay groove 5, mounting groove 6 and actinograph 9; Described the Lights section is made up of light source 1, dimmer 2 and light source slot 3; Described light source 1 is commercially available incandescent lamp bulb or small-sized daylight lamp, is placed in the airtight light source slot 3; The light intensity that dimmer 2 is regulated light source 1; The front end face of light source slot 3 is provided with light hole 4; Mounting groove 6 is a square groove, is close to the front end that light source slot 3 has light hole 4; Mounting groove 6 front end faces are provided with the light hole 4 corresponding light holes 7 with light source slot 3 front end faces; Decay groove 5 is a square groove, is placed within the mounting groove 6, and its former and later two transparent surfaces 51 and 52 are made by optical glass, and all the other each faces are made by organic glass; Actinograph 9 is commercially available photosynthetically active radiation meter or illuminometer, and its light probe 8 is positioned at the light hole 7 of mounting groove 6 front end faces, is used to measure luminous energy in the variation through its intensity before and after the attenuation medium.
During use, open earlier light source 1, regulate light source regulator 2 and make light intensity reach appropriate brightness, the light probe 8 of photosynthetically active radiation meter or illuminometer is placed in the light hole 7, measure the light intensity before the decay; The decay groove 5 that will fill algae liquid afterwards places in the mounting groove 6, measures the intensity of light intensity behind the process attenuation medium.Change algae liquid concentration and decay groove optical path (width) more successively and continue to measure, can obtain the pad value of a series of algae liquid.
The effect of utility model is as follows:
The light intensity attenuation determinator that provides with the utility model can be measured under the condition of microalgae cell Different Light intensity, light path and variable concentrations the attenuation to light intensity.Have that light intensity is adjustable, easy operation, cleaning are convenient, the result accurately, characteristics such as favorable reproducibility, be applicable under laboratory condition, measure little algae and other photosynthetic organism cell culture system be subjected to light and light intensity attenuation situation.
Further describe the utility model below in conjunction with drawings and Examples:
Accompanying drawing 1 is a structural representation of the present utility model;
Accompanying drawing 2 is the structural representation of the Lights section;
Accompanying drawing 3 is the structural representation of decay groove 5;
Accompanying drawing 4 is the structural representation of mounting groove 6;
Accompanying drawing 5 is the structural representation of actinograph 9;
Wherein: light source 1 dimmer 2 light source slots 3
Light hole 4 decay grooves 5 mounting grooves 6
Light hole 7 light probes 8 actinographs 9
As seen from the figure, the light intensity attenuation determinator that the utility model provides comprises the Lights section, decay groove 5, mounting groove 6 and actinograph 9; Described the Lights section is made up of light source 1, dimmer 2 and light source slot 3; Light source 1 is commercially available incandescent lamp bulb or small-sized daylight lamp, is placed in the airtight light source slot 3; The light intensity that dimmer 2 is regulated light source 1; The front end face of light source slot 3 is provided with light hole 4; Mounting groove 6 is a square groove, is close to the front end of the light hole 4 of light source slot 3; Mounting groove 6 front ends are provided with the light hole 4 corresponding light holes 7 with light source slot 3 front end faces; Decay groove 5 is a square groove, places within the mounting groove 6; Decay groove 5 former and later two transparent surfaces 51 and 52 are made by optical glass, and all the other each faces are made by organic glass; Actinograph 9 is commercially available photosynthetically active radiation meter or illuminometer, and its light probe 8 is positioned at the light hole 7 of mounting groove 6 front end faces, is used to measure luminous energy intensity variations behind the process attenuation medium.
The actinograph 9 of present embodiment is commercially available photosynthetically active radiation meter (also can be illuminometer certainly), is used to measure luminous energy in the variation through its intensity before and after the attenuation medium; Light source 1 is selected 100 watts of (about 3.32mW/cm for use 2) incandescent lamp bulb; Light source slot 3 length, width and highly be respectively 30cm, 20cm and 12cm; The radius of the light hole 4 of light source slot 3 front end faces is 1.7cm (identical with the diameter of the light probe 8 of actinograph 9); Glass decay groove 5 is made by optical glass, its length and highly be respectively 10cm and 4cm, and its width to be light path be respectively 1,2,3,4,5cm; Optical glass thickness 0.1cm; Glass decay groove 5 places in the mounting groove 6 of corresponding size; Spirulina cells concentration in the glass decay groove 5 is OD 560=0.1-2.0; Actinograph 9 is a FGH-10 type photosynthetically active radiation meter.Measured Different Light intensity, different frustule concentration during with different light path light intensity and attenuation results thereof and coincide good according to the value that theoretical formula (Lambert-Beer law) calculates.

Claims (3)

1. a light intensity attenuation determinator is characterized in that, comprises the Lights section, decay groove (5), mounting groove (6) and actinograph (9); The Lights section is made up of light source (1), dimmer (2) and light source slot (3); Light source (1) is placed in the airtight light source slot (3), and dimmer (2) is regulated the light intensity of light source (1), and the front end face of light source slot (3) is provided with light hole (4); Mounting groove (6) is a square groove, is close to the front end of the light hole (4) of light source slot (3); Mounting groove (6) front end face is provided with the corresponding light hole of light hole (4) (7) with light source slot (3) front end face; Square decay groove (5) is positioned among the mounting groove (6), and its former and later two transparent surfaces (51) and (52) are made by optical glass, and all the other each faces are made by organic glass or optical glass; The light probe (8) of actinograph (9) is inserted in the light hole (7) on mounting groove (6) front end face.
2. by the described light intensity attenuation determinator of claim 1, it is characterized in that actinograph (9) is commercially available photosynthetically active radiation meter or illuminometer.
3. by the described light intensity attenuation determinator of claim 1, it is characterized in that described light source (1) is commercially available incandescent lamp bulb or small-sized daylight lamp.
CN 01207695 2001-03-23 2001-03-23 Light intensity attenuation determination instrument Expired - Fee Related CN2475023Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 01207695 CN2475023Y (en) 2001-03-23 2001-03-23 Light intensity attenuation determination instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 01207695 CN2475023Y (en) 2001-03-23 2001-03-23 Light intensity attenuation determination instrument

Publications (1)

Publication Number Publication Date
CN2475023Y true CN2475023Y (en) 2002-01-30

Family

ID=33628019

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 01207695 Expired - Fee Related CN2475023Y (en) 2001-03-23 2001-03-23 Light intensity attenuation determination instrument

Country Status (1)

Country Link
CN (1) CN2475023Y (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104614324A (en) * 2015-03-04 2015-05-13 哈尔滨工业大学 Method for measuring optical constant of microalgae culture medium and spectral attenuation coefficient of microalgae in combined way
CN106053025A (en) * 2016-06-30 2016-10-26 河南科技大学 Measuring device and method for Airy beam attenuation factor

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104614324A (en) * 2015-03-04 2015-05-13 哈尔滨工业大学 Method for measuring optical constant of microalgae culture medium and spectral attenuation coefficient of microalgae in combined way
CN104614324B (en) * 2015-03-04 2017-08-25 哈尔滨工业大学 The union measuring method of micro-algae culture medium optical constant and microalgae Spectrum attenuation coefficient
CN106053025A (en) * 2016-06-30 2016-10-26 河南科技大学 Measuring device and method for Airy beam attenuation factor
CN106053025B (en) * 2016-06-30 2018-09-14 河南科技大学 A kind of measuring device and method of the Airy beam attenuations factor

Similar Documents

Publication Publication Date Title
Maddux et al. Some Interactions of Temperature, Light Intensity, and Nutrient Concentration during the Continuous Culture of Nitzschia Closterium and Tetraselmis SP 1
Mouget et al. Suitability of the fluorescence monitoring system (FMS, Hansatech) for measurement of photosynthetic characteristics in algae
US11261474B2 (en) Optical device for in-line and real-time monitoring of microorganisms
Kromkamp et al. Short-term variations in photosynthetic parameters of Nannochloropsis cultures grown in two types of outdoor mass cultivation systems
CA2448390A1 (en) Semen analysis
Skarstad et al. Cell cycle analysis of microorganisms
CN104335830A (en) System and method for supplementing light for gerbera hybrida
CN106053350A (en) A micro-nanofiber humidity sensor based on tungsten disulfide and a preparing method thereof
CN2475023Y (en) Light intensity attenuation determination instrument
WO1979000282A1 (en) Method of dispersing a light beam in a liquid culture medium
Bruyant et al. An axenic cyclostat of Prochlorococcus PCC 9511 with a simulator of natural light regimes
CN106290162B (en) A kind of device and method of continuous detection bacterium growth curve
CN215339529U (en) Multifunctional microorganism growth curve tester
CN110806404A (en) Improved chlorophyll fluorescence detection method and device
CN112834493B (en) Blue fluorescent carbon dot, preparation method and application
CN206348272U (en) A kind of kit for anti-interference quick detection of microbe quantity by bioluminescence method
CN1793852A (en) Multiwave length spectro state carbon dioxide automatic monitoring device
CN219475462U (en) Black plastic film heat-resisting perforation capability testing arrangement
CN211627355U (en) Device for measuring microalgae suspension light attenuation coefficient
CN207439938U (en) A kind of fluorescent tracing detector
RU96573U1 (en) DEVICE FOR BIOTESTING
CN104458615B (en) Preparation method of photonic crystal total-reflection layer and total-reflection layer-based fast detector for total number of bacteria
CN218381290U (en) Automatic illuminometer calibrating device based on laser range finder
CN101634755B (en) Optical adjusting plate
CN213779882U (en) Detection device with temperature capable of being adjusted quickly

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
C19 Lapse of patent right due to non-payment of the annual fee
CF01 Termination of patent right due to non-payment of annual fee