CN215339529U - Multifunctional microorganism growth curve tester - Google Patents
Multifunctional microorganism growth curve tester Download PDFInfo
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- CN215339529U CN215339529U CN202121630262.8U CN202121630262U CN215339529U CN 215339529 U CN215339529 U CN 215339529U CN 202121630262 U CN202121630262 U CN 202121630262U CN 215339529 U CN215339529 U CN 215339529U
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Abstract
The utility model relates to the technical field of microorganism scientific research equipment, in particular to a multifunctional microorganism growth curve tester, which comprises an enzyme-labeling instrument host; be provided with the display screen on the ELIASA host computer, the sample detection room with advance the kind storehouse, the top in advance kind storehouse is provided with CCD module of shooing, the inside in advance kind storehouse is provided with first temperature control module, the indoor light absorption and fluorescence detection module that is provided with of sample detection, second temperature control module, mechanical oscillation module, gas control system, multiband lighting source and second honeycomb plate collet, it is provided with the transfer guide rail from the entrance to inside extension to advance kind in the storehouse, first honeycomb plate collet is installed on transfer guide rail, it is first, second honeycomb plate collet all can be used to install the ELIAS orifice plate, it is used for on ELIAS orifice plate propelling movement to second honeycomb plate collet still to be provided with the propelling movement structure in the storehouse to advance kind. In practical application, the multifunctional characteristic of the tester is utilized to test the growth curve of the microorganism under different conditions.
Description
Technical Field
The utility model relates to the technical field of microorganism scientific research equipment, in particular to a multifunctional microorganism growth curve tester.
Background
The growth of microorganisms generally refers to an increase in the number of cells, and a microorganism growth curve is a curve in which the growth amount of microorganisms is plotted on the ordinate and the culture time is plotted on the abscissa, and represents the dynamics of the overall process from the growth and reproduction of bacteria to the death by senescence. The growth of microorganisms has certain regularity, and a typical microorganism growth curve comprises four periods: the growth curve can measure the metabolic condition of microorganisms in different growth conditions, the growth trend of the microorganisms and other important indexes.
The microorganism growth curve determinator commonly used in the prior art is designed based on a turbidimetric method and further based on the principle of photometry absorption of an ultraviolet-visible spectrophotometer, and has four commercialized models, namely BIOSCREEN C and BIOSCREEN CPRO of an upgraded version produced in Finland, BMG-GCM produced in Germany, SCIENTZ-WSQ produced in Ningbo New Ganoderma and BIOSAN RTS-1 and RTS-1C produced in Laplace, but the four models have corresponding defects, for example, the BIOSCREEN C and the BIOSCREEN CPRO of the upgraded version have the defects that the price of an imported instrument is high, only a consumable 100-hole microporous plate fixed by a manufacturer can be used, and other microporous plates cannot be used. The instrument designed based on the microporous plate and having the German model BMG-GCM is developed and produced in nearly two years, is further improved on the basis of an enzyme labeling instrument, and has the defect that the light absorption value measured by the instrument is not the standard OD600 in the strict sense, and is different from the value measured by a standard cuvette of an ultraviolet visible spectrophotometer because the light absorption is related to the optical path, namely the thickness of an absorption layer. The standard optical path of the UV spectrophotometer is 1cm, and the optical path of the instrument is highly correlated with the wells of the microplate. RTS-1 and RTS-1C have the defects that 1 instrument can only detect 1 channel at a time by using a 50mL sterile tube matched with a manufacturer, the flux is too low, and the growth states of different bacteria cannot be compared at the same time. SCIENTZ-WSQ has the disadvantages that the consumption of bacteria solution is large when using test tubes and the bacteria cannot be shaken in the test tubes because of the fixed adapter, so that the bacteria shaking is insufficient.
Meanwhile, the tester has a single function and cannot be used under various conditions, so that a multifunctional microorganism growth curve tester overcoming the defects is urgently needed to better research the biological characteristics of microorganisms.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems, the utility model provides a multifunctional microorganism growth curve tester based on the improvement of an enzyme-labeling instrument with the main structure and the working principle basically the same as those of a spectrophotometer.
The technical scheme of the utility model is as follows:
a multifunctional microorganism growth curve tester comprises an enzyme-labeling instrument host;
the microplate reader host is provided with a display screen, a sample detection chamber and a sample introduction chamber;
a CCD camera module is arranged above the sample feeding bin, a first temperature control module is arranged at the inner bottom of the sample feeding bin,
a light absorption and fluorescence detection module, a second temperature control module, a mechanical oscillation module, a gas control system, a multiband illumination light source and a second honeycomb plate bottom support are arranged in the sample detection chamber;
wherein, be provided with the conveying guide rail of inwards extending from the entrance in advancing the kind storehouse, first honeycomb panel collet is installed on the conveying guide rail, first honeycomb panel collet with the second honeycomb panel collet all can be used to install the ELIAS orifice plate, still be provided with push structure in advancing the kind storehouse in order to be used for with on the first honeycomb panel collet the ELIAS orifice plate propelling movement extremely on the second honeycomb panel collet.
Preferably, the first honeycomb panel collet is the same as the second honeycomb panel collet in model, and the first honeycomb panel collet is provided with a hollow-out portion on the second honeycomb panel collet.
Preferably, the first honeycomb plate bottom support and the second honeycomb plate bottom support are provided with grooves, the enzyme-labeled pore plate is provided with a protruding structure, and the enzyme-labeled pore plate is installed on the first honeycomb plate bottom support and the second honeycomb plate bottom support through the protruding structure.
Preferably, the second honeycomb plate bottom support and the interior of the sample feeding bin can be modified as required to accommodate different types and different numbers of the enzyme-labeled hole plates.
Preferably, the top of the sample detection chamber is provided with the multiband illumination light source, wherein the number of the multiband illumination light source is increased according to the number of the elisa pore plates, and black baffles can be used between different elisa pore plates to block light.
Preferably, the gas control system comprises a gas outlet and a gas inlet, the gas outlet and the gas inlet are arranged on the side wall of the sample detection chamber, and the gas outlet and the gas inlet are respectively provided with a flow meter.
Preferably, the mechanical oscillation module is installed at the bottom of the sample detection chamber, and the second honeycomb plate base is installed above the mechanical oscillation module.
Preferably, the oscillation speed of the mechanical oscillation module is 0-300 rpm/min.
Preferably, the first temperature control module and the second temperature control module have the same model, and the temperature control precision of the first temperature control module and the second temperature control module is +/-0.05-0.15 ℃.
Preferably, the light absorption and fluorescence detection module includes a broadband filter, and the wavelength band of the broadband filter is 400-580 nm.
According to the technical scheme, the first temperature control module and the second temperature control module of the multifunctional microorganism growth curve tester can keep the temperature of the sample feeding bin and the sample detection chamber constant, the CCD camera module can be used for analyzing microorganisms when different exogenous compounds are added according to the principle of microorganism metabolism color reaction, the light absorption and fluorescence detection module can be used for monitoring fluorescent protein strains or microorganisms with fluorescence, the mechanical oscillation module can be used for simulating oscillation with different degrees, the gas control system can meet the measurement requirements of different types of strains, the multi-band illumination light source can be used for simulating light sources with different bands, or the combination of the functional modules is used, the multifunctional microorganism growth curve tester can be suitable for measuring the growth curve of the microorganisms under different conditions based on strong multi-band functional characteristics, and is favorable for researching the growth characteristics of the microorganisms, meanwhile, the method has good application prospect.
Drawings
FIG. 1 is a schematic structural diagram of a multifunctional microorganism growth curve tester.
Description of the reference numerals
A microplate reader host 1; a display screen 2; a sample detection chamber 3; an air inlet 4; an air outlet 5;
a multiband illumination light source 6; a sample introduction bin 7; a CCD camera module 8; a first cellular board shoe 9.
Detailed Description
The following detailed description of embodiments of the utility model refers to the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating embodiments of the utility model, are given by way of illustration and explanation only, not limitation.
In the present invention, the terms of orientation such as "upper, lower, left and right" used herein generally refer to the upper, lower, left and right directions of the multifunctional microorganism growth curve measuring apparatus shown in FIG. 1, unless otherwise specified.
The utility model provides a multifunctional microorganism growth curve tester, as shown in figure 1, the multifunctional microorganism growth curve tester comprises an enzyme-labeling instrument host 1;
the microplate reader host 1 is provided with a display screen 2, a sample detection chamber 3 and a sample introduction chamber 7;
a CCD camera module 8 is arranged above the sample feeding bin 7, a first temperature control module is arranged at the inner bottom of the sample feeding bin 7,
a light absorption and fluorescence detection module, a second temperature control module, a mechanical oscillation module, a gas control system, a multiband illumination light source 6 and a second honeycomb plate bottom support are arranged in the sample detection chamber 3;
wherein, be provided with the conveying guide rail that inwards extends from the entrance in advancing kind storehouse 7, first honeycomb panel collet 9 is installed on the conveying guide rail, first honeycomb panel collet 9 with the second honeycomb panel collet all can be used to install the enzyme mark orifice plate, it still is provided with push structure in the storehouse 7 to advance in order to be used for with on the first honeycomb panel collet 9 the enzyme mark orifice plate propelling movement extremely on the second honeycomb panel collet.
According to the technical scheme, the first temperature control module and the second temperature control module of the multifunctional microorganism growth curve tester can keep the temperature of the sample feeding bin and the sample detection chamber constant, the CCD camera module can be used for analyzing microorganisms when different exogenous compounds are added according to the principle of microorganism metabolism color reaction, the light absorption and fluorescence detection module can be used for monitoring fluorescent protein strains or microorganisms with fluorescence, the mechanical oscillation module can be used for simulating oscillation with different degrees, the gas control system can meet the measurement requirements of different types of strains, the multi-band illumination light source can be used for simulating light sources with different bands, or the combination of the functional modules is used, the multifunctional microorganism growth curve tester can be suitable for measuring the growth curve of the microorganisms under different conditions based on strong multi-band functional characteristics, and is favorable for researching the growth characteristics of the microorganisms, meanwhile, the method has good application prospect.
According to a preferred embodiment, the first honeycomb plate cork base 9 and the second honeycomb plate cork base are the same in model, and the first honeycomb plate cork base 9 and the second honeycomb plate cork base are provided with hollows.
Furthermore, the first honeycomb plate bottom support 9 and the second honeycomb plate bottom support are provided with grooves, the enzyme-labeled pore plate is provided with a protruding structure, and the enzyme-labeled pore plate is installed on the first honeycomb plate bottom support 9 and the second honeycomb plate bottom support through the protruding structure.
Further, the second honeycomb plate bottom support and the interior of the sample feeding bin can be modified as required to accommodate the enzyme-labeled hole plates with different types and different quantities.
In the embodiment of the utility model, the first honeycomb plate 9 and the second honeycomb plate have the same model, and the first honeycomb plate base 9 and the second honeycomb plate base are provided with hollows to facilitate the installation of the elisa pore plate, wherein the first honeycomb plate base 9 can move on the conveying guide rail to convey the elisa pore plate installed on the first honeycomb plate base 9 into the sample feeding bin 7. Further, in order to perform experiments with different types and different numbers of the elisa pore plates, the second honeycomb plate bottom support and the interior of the sample feeding bin can be modified to accommodate different types and different numbers of the elisa pore plates. The common types of the first honeycomb plate bottom support 9 and the second honeycomb plate bottom support comprise 96-hole, 48-hole, 24-hole, 12-hole and 6-hole honeycomb plate bottom supports, the common types of the enzyme-labeled pore plates comprise 96-hole, 48-hole, 24-hole, 12-hole and 6-hole enzyme-labeled pore plates, the number of the enzyme-labeled pore plates can be 1, 2, 3 or more, in the using process, the first honeycomb plate bottom support 9, the second honeycomb plate bottom support and the enzyme-labeled pore plates need to be matched and correspondingly used, as shown in fig. 1, the sample injection bin 7 can simultaneously contain 3 96-hole enzyme-labeled pore plates, the corresponding first honeycomb plate bottom support 9 with 3 96-hole, and the second honeycomb plate bottom support in the sample detection chamber 3 is provided with 3 96-hole second honeycomb plates. Further, as shown in fig. 1, when there are a plurality of elisa pore plates, the conveying guide rail inside the sample injection bin 7 can be further modified, so that each elisa pore plate can be automatically placed below the CCD photographing module 8 according to the need.
According to a preferred embodiment, the multiband illumination light sources 6 are arranged on the top of the sample detection chamber 3, wherein the number of the multiband illumination light sources 6 is increased according to the number of the elisa pore plates, and black baffles can be used between different elisa pore plates to block light.
In the embodiment of the present invention, the multiband illumination light source 6 can select red light, blue light, white light, etc. with fixed range of wavelength bands through a filter, etc. to be used for studying the effect of different bacteria on growth under the condition of having or not illuminating or different wavelength band light sources, and suitable illumination conditions are searched, for example, the action mechanism of some bacteria is anti-illumination, the action mechanism of some bacteria is absorption illumination, wherein the bacteria absorbing illumination are representative of photosynthetic bacteria. Furthermore, the number of the multiband illumination light sources 6 can be increased correspondingly according to different types and numbers of the enzyme-labeled pore plates, so that each detection area of the enzyme-labeled pore plate can be provided with a plurality of multiband illumination light sources 6 according to requirements, and a black baffle plate can be used between different detection areas of the enzyme-labeled pore plate to block light.
According to a preferred embodiment, the gas control system comprises a gas outlet 5 and a gas inlet 4, the gas outlet 5 and the gas inlet 4 being provided on the side wall of the sample detection chamber 3, the gas outlet 5 and the gas inlet 4 being provided with a flow meter, respectively.
In the embodiment of the utility model, the gas control system can be introduced with gases such as oxygen, carbon dioxide, nitrogen and the like with different concentrations, and can provide a gas environment with required concentration as required for growth curve determination of different types of microorganisms such as oxygen consumption type, anaerobic type, facultative anaerobic type and the like. The flowmeters arranged at the gas outlet 5 and the gas inlet 4 can detect the gas flow in real time. Further, according to different types and different quantities of the enzyme-labeled pore plates, the gas control system can preferably control the detection area of each enzyme-labeled pore plate independently.
According to a preferred embodiment, the mechanical oscillation module is installed at the bottom of the sample detection chamber 3, and the second honeycomb plate base is installed above the mechanical oscillation module.
Further, the oscillation speed of the oscillation module is 0-300 rpm/min.
In the embodiment of the utility model, the second honeycomb plate bottom support is arranged above the mechanical oscillation module, so that the enzyme-labeled pore plate can be oscillated better, meanwhile, the oscillation mode of the mechanical oscillation module can be adjusted, and the oscillation mode can be set as required in practical application so as to measure the microorganism growth curves at different oscillation speeds. Wherein the adjusting range of the oscillation speed is 0-300rpm/min, preferably 0-250 rpm/min.
According to a preferred embodiment, the first temperature control module and the second temperature control module have the same model, and the temperature control precision of the first temperature control module and the second temperature control module is +/-0.05-0.15 ℃.
In the embodiment of the utility model, the first temperature control module and the second temperature control module have the same model, which is beneficial to ensuring the consistency of the temperature inside the sample introduction chamber 3 and the temperature inside the sample detection chamber, so that the influence of the temperature is eliminated to the maximum extent, and the experimental accuracy is improved. The temperature control precision of the first temperature control module and the second temperature control module is +/-0.05-0.15 ℃, and preferably +/-0.1 ℃. Furthermore, the second temperature control module is matched with the mechanical oscillation module for use, so that the heat productivity generated by long-time oscillation and the abrasion of an instrument can be effectively reduced.
According to a preferred embodiment, the light absorption and fluorescence detection module comprises a broadband filter, and the wavelength band of the broadband filter is 400-580 nm.
In the embodiment of the utility model, the broadband optical filter is a group added on the optical filter selected for light absorption determination of a common microplate reader, the waveband of the broadband optical filter is preferably 420-580nm so as to be used for determination research of a microorganism growth curve, and meanwhile, the light absorption result obtained by the broadband optical filter is not influenced by the color change of a growth culture medium, and the measurement result only shows the turbidity condition of a suspension liquid, so that the growth condition of microorganisms can be objectively reflected. Further, when the light absorption and fluorescence detection module is used for measuring the light absorption value, and when the height of an enzyme-labeled hole of the enzyme-labeled hole plate is not consistent with the standard optical path of 1cm, the standard detection value is converted according to a calculation formula. When a fluorescent protein strain or a microorganism with fluorescence is monitored, for example, a strain containing a GFP/eGFP fluorescent label can be detected by adopting an excitation wavelength of 485-495nm, preferably 488nm and an emission wavelength of 500-510nm, preferably 507nm based on the light absorption and fluorescence detection module, and the fluorescence detection is carried out by using a black enzyme-labeled pore plate.
In the utility model, the determination process of the microbial growth curve comprises the following steps: under the aseptic environment, the enzyme-labeled pore plate is arranged on the first honeycomb plate base 9, bacteria liquid is added on the sterile transparent enzyme-labeled pore plate, the enzyme-labeled pore plate enters the sample introduction bin 7 under the action of the transmission guide rail, the sample hole, the total detection time, the interval detection time, the temperature, the oscillation mode, the oscillation speed, the detection light absorption wavelength and the like of detection are set through a control system, then the enzyme-labeled pore plate is pushed from the sample introduction bin 7 to the second honeycomb plate base for detection, if the illumination condition is needed, the illumination wavelength, the illumination intensity of the corresponding area or the interval time of the multiband illumination light source 6 is set, if oxygen control or facultative anaerobe or strict anaerobe is needed, the gas control system can be opened for corresponding setting, and after the measurement is finished, the enzyme-labeled pore plate is returned to the first honeycomb plate base 9 and taken out, and deriving growth curve data for further analysis, displayed in real time on the display screen.
Further, when the condition that different exogenous compounds are added to the microorganisms in the sample introduction bin 7 needs to be subjected to phenotype analysis by CCD photographing, after a proper temperature is set, a corresponding execution program can be directly selected, automatic photographing can be set at a fixed time interval such as 15min or 30min, and after the color intensity is calculated by a control system, each set hole on the enzyme-labeled pore plate can form a dynamic curve on the display screen so as to reflect the change of energy metabolism. Among them, the CCD photograph has an advantage in that the change of metabolic intensity can be visually seen with naked eyes.
The multifunctional microorganism growth curve tester provided by the utility model is based on strong functional characteristics, can be suitable for testing the growth curve of microorganisms under different conditions, is beneficial to researching the growth characteristics of the microorganisms, and has good application prospects.
The preferred embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto. Within the scope of the technical idea of the utility model, many simple modifications may be made to the technical solution of the utility model, and in order to avoid unnecessary repetition, various possible combinations of the utility model will not be described further. Such simple modifications and combinations should be considered within the scope of the present disclosure as well.
Claims (10)
1. The multifunctional microorganism growth curve tester is characterized by comprising a microplate reader host (1);
the microplate reader host (1) is provided with a display screen (2), a sample detection chamber (3) and a sample introduction bin (7);
a CCD photographing module (8) is arranged above the sample feeding bin (7), and a first temperature control module is arranged inside the sample feeding bin (7);
a light absorption and fluorescence detection module, a second temperature control module, a mechanical oscillation module, a gas control system, a multiband illumination light source (6) and a second honeycomb plate bottom support are arranged in the sample detection chamber (3);
wherein, be provided with in advance kind storehouse (7) from the inside conveying guide rail that extends of entrance, first honeycomb panel collet (9) is installed on the conveying guide rail, first honeycomb panel collet (9) with the second honeycomb panel collet all can be used to install the enzyme mark orifice plate, still be provided with in advance kind storehouse (7) push structure in order to be used for with on first honeycomb panel collet (9) the enzyme mark orifice plate propelling movement extremely on the second honeycomb panel collet.
2. The multifunctional microorganism growth curve tester according to claim 1, wherein the first honeycomb plate cork base (9) and the second honeycomb plate cork base are the same in size, and the first honeycomb plate cork base (9) and the second honeycomb plate cork base are provided with hollows.
3. The multifunctional microorganism growth curve tester according to claim 2, wherein the first honeycomb plate base (9) and the second honeycomb plate base are provided with grooves, the enzyme-labeled pore plate is provided with a protruding structure, and the enzyme-labeled pore plate is mounted on the first honeycomb plate base (9) and the second honeycomb plate base through the protruding structure.
4. The multifunctional microorganism growth curve analyzer as claimed in claim 1, wherein the second honeycomb plate bottom support and the interior of the sample feeding bin can be modified as required for accommodating different types and different numbers of the enzyme-labeled hole plates.
5. The multifunctional microorganism growth curve tester according to claim 4, characterized in that the top of the sample detection chamber (3) is provided with the multiband illumination light source (6);
the number of the multiband illumination light sources (6) is increased according to the number of the enzyme-labeled pore plates, and black baffles can be used between different enzyme-labeled pore plates to block light.
6. The multifunctional microorganism growth curve tester according to claim 1, characterized in that the gas control system comprises a gas outlet (5) and a gas inlet (4), the gas outlet (5) and the gas inlet (4) are opened on the side wall of the sample detection chamber (3), and the gas outlet (5) and the gas inlet (4) are respectively provided with a flow meter.
7. The multifunctional microorganism growth curve tester according to claim 1, wherein the mechanical oscillation module is installed at the bottom of the sample detection chamber (3), and the second honeycomb plate base is installed above the mechanical oscillation module.
8. The multifunctional microorganism growth curve analyzer as claimed in claim 7, wherein the oscillation speed of the mechanical oscillation module is 0-300 rpm/min.
9. The multifunctional microorganism growth curve analyzer of claim 1, wherein the first temperature control module and the second temperature control module are the same type;
the temperature control precision of the first temperature control module and the second temperature control module is +/-0.05-0.15 ℃.
10. The multifunctional microorganism growth curve tester as claimed in claim 1, wherein the light absorption and fluorescence detection module comprises a broadband filter, and the wavelength band of the broadband filter is 400-580 nm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2023178668A1 (en) * | 2022-03-25 | 2023-09-28 | 深圳先进技术研究院 | Microbial growth curve determination apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2023178668A1 (en) * | 2022-03-25 | 2023-09-28 | 深圳先进技术研究院 | Microbial growth curve determination apparatus |
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