JPH0723315B2 - Blood sugar elevation inhibitor - Google Patents

Description

TECHNICAL FIELD The present invention relates to a substance for suppressing blood sugar level elevation, which is mainly composed of hemicellulose extracted from the hulls of grains and beans.

"Prior art and its problems" In recent years, it has been reported that guar gum, tragacantham gum, pectin, methylcellulose, konjak mannan and the like have an effect of suppressing an increase in blood glucose level, and has been attracting attention as a diet therapy for diabetic patients and the like. The reason why these high molecular weight substances have the effect of suppressing the increase in blood sugar level is not clear yet, but these high molecular weight substances adsorb sugar in the body,
It is considered to inhibit or delay its absorption.

However, in the case of the above-mentioned polymer substances, for example, even if ingested as a powder, it is not possible to obtain an effective inhibitory effect on the rise in blood glucose level, and in order to obtain a sufficient effect, it is necessary to ingest it in a gel state. . However, although these polymer substances are originally supplied as powder products, they have the drawback that they have hygroscopicity, high viscosity, and are unlikely to form a homogeneous gel when dispersed in water. And when gelled, the volume generally increases, so it is easy to accumulate in the belly,
It has been difficult for diabetic patients to take on a regular basis. In addition, it has been inconvenient to handle because it has to be gelled before ingestion.

"Object of the invention" The object of the present invention is, in view of the above problems of the prior art, even powder is very soluble in water, excellent in water dispersibility, easy to be a homogeneous solution, easy to eat, effective in a small amount, Moreover, it is to provide a substance for suppressing an increase in blood glucose level that does not require complicated adjustment.

“Structure of the Invention” The present inventors have been conducting research for many years on the physiologically active action of dietary fiber such as corn fiber. In the process, an extract obtained by alkali extraction from dietary fiber (hemicellulose is the main component). The present invention has been completed based on the finding that a small amount of the compound exhibits a remarkable inhibitory effect on blood sugar level elevation.

That is, the blood sugar elevation-inhibiting substance of the present invention is the outer skin of cereals or beans, or the rest of starch or protein, lipids, minerals and the like removed from the outer skin of cereals or beans, with hemicellulose obtained by alkali extraction as the main component. To do.

The blood sugar level elevation-inhibiting substance of the present invention exhibits an effective blood glucose level elevation-inhibiting action by taking a small amount in either liquid or powder state. Therefore, it is easy to handle and easy to eat,
It is practical.

As a raw material for the substance for suppressing blood sugar level elevation of the present invention, the outer coat of cereals or beans is used. As the outer coat of cereals, for example, the outer coat of corn, rice bran, wheat bran, barley bran, hato bran, oat bran, rye bran, etc. can be used. Also, as the hull of beans, for example, soybean,
The outer skin of red beans and peas can be used.

In the present invention, the hulls of these cereals or beans may be alkali-extracted as they are, but it is preferable to carry out the alkali-extraction using the residue obtained by removing the starch, protein, lipid, minerals, etc. from the hulls of these cereals or beans. This is preferable because subsequent extraction and purification will be easy. The method for removing starch, proteins, lipids, minerals and the like from the hulls of cereals or beans may be any of enzymatic treatment, chemical treatment and physical treatment, or may be a combination of these treatments. Enzymatic treatment is carried out by using, for example, starch degrading enzymes such as α-amylase and glucoamylase, proteolytic enzymes such as protease, lipid degrading enzymes such as lipase, and fibrinolytic enzymes such as cellulase.
It is carried out by adding and treating under conditions of 3 to 9 and temperature of 30 to 100 ° C. Chemical treatment is carried out by adding an aqueous solution of mineral acid or organic acid to the hulls of the above-mentioned cereals or beans, and adding PH2
It is carried out by heating under the conditions of 5 to 5 or by adding a food grade surfactant and heat treating under the conditions of PH 3 to 8. Physical treatment, after crushing the outer skin of the grain or beans with a homogenizer, a crusher such as a hammer mill,
It is carried out by sieving. Alternatively, the outer skin of the grain or legume may be extracted with alkali as it is, and then subjected to the above-mentioned enzyme treatment, chemical treatment or physical treatment.

The alkali extraction is carried out by adding an alkaline aqueous solution such as an aqueous sodium hydroxide solution and mixing them to elute the non-cellulosic polysaccharides. The substance for suppressing increase in blood sugar level of the present invention can be obtained by neutralizing this alkaline extract as unpurified, or by further purifying the neutralized product by the following procedure. That is, proteins precipitated by neutralization are separated and removed by means such as centrifugation, and the supernatant is dialyzed, ethyl alcohol, ion exchange resin membrane treatment, ultrapermeabilization treatment, alcohol purification, agent The substance for suppressing an increase in blood sugar level of the present invention containing a hemicellulose component having an arbitrary purity can be obtained by purification by treatment alone or in combination as appropriate.

For example, the blood sugar level elevation-inhibiting substance of the present invention obtained as described above comprises hemicellulose as a main component and a slight amount of lignin, cellulose, ash and the like contained therein. And, even if a small amount is used as it is, it shows an effective inhibitory effect on blood sugar level elevation. Therefore, it can be used as it is as a healthy food and drink or a medicine, and by adding a small amount to the food or drink, it is possible to impart physiological activity without impairing the flavor or texture of the food or drink.

"Examples of the Invention" Example 1 100 g of corn fiber (corn hull) is placed in a 5-volume Erlenmeyer flask. Glucoamylase (Nagase & Co., Ltd.)
(1 × 10 ⁴ GUN / g) 5g dissolved in distilled water 4 and passed over paper, 0.2M acetate buffer solution (PH4.8) 1 was added to the solution to prepare glucoamylase solution 5 and a few drops of toluene Was added to the above cone fiber and kept at 40 ° C. for 24 hours. Pass this through a glass filter (151G3), wash the residue with water, transfer to a 2 volume Erlenmeyer flask, add 0.5N sodium hydroxide solution 1, fill the container with nitrogen glass, and seal with a rubber stopper. The alkali-soluble non-cellulosic polysaccharide fraction was extracted by shaking (130 strokes / min) at room temperature for 18 hours. This product was centrifuged (3,000 rpm, 10 minutes), the liquid part was neutralized with glacial acetic acid, and trichloracetic acid was added to a final concentration of 7% to precipitate the protein. Centrifuge the precipitate (5000 rpm, 10 minutes)
Water was added to the obtained separated liquid (about 700 ml) to make it about 1.5, and the mixture was dialyzed in running water for 3 days using a cellophane tube. After confirming that the dialysis contents became neutral, about 4 times the volume of ethyl alcohol (final concentration 80
% Or more) and allowed to stand overnight to sufficiently form a precipitate. This precipitate was collected by centrifugation (4000 rpm, 10 minutes), dissolved in distilled water 1 and freeze-dried to obtain 13 g of a light-colored product of the present invention. This was made into powder and used for the experiment. Its composition is shown in Table 1. The composition was analyzed according to the Southgate method.

Example 2 Wheat bran was added to water so as to be 10%, treated with hyscotron for 10 minutes, and then sieved using a 48 mesh sieve while thoroughly washing with running water. 2 parts of the thus-obtained bran on a sieve are put into 10 parts of a 2% sodium hydroxide solution, and a hemicellulose component is extracted while stirring this. After completion of the extraction, the solution is neutralized with hydrochloric acid and centrifuged to obtain the supernatant. The supernatant was decolorized with an adsorption resin, desalted with an ion exchange resin, further treated with activated carbon, concentrated and freeze-dried to obtain the product of the present invention. The composition is second
Shown in the table. Compositional analysis followed the Southgate method. In the above, the drying can also be performed using a device such as a spray dryer or a drum dryer.

Test Example Rats (10-week-old, Wistar system, male, body weight of about 250 g) were fasted for 24 hours, and blood was collected from the rats for 24 hours to measure the fasting blood glucose level. Then, 1.25 ml of the test solution per 100 g of body weight was orally administered using an oral probe. 15 minutes, 30 minutes, 60 minutes after oral administration,
Blood was collected at 120 minutes, and the blood glucose level was measured.

The test solution is a control (20% glucose solution),
2% of corn hemicellulose (Example 1) or wheat bran hemicellulose (Example 2) was used.

The blood glucose level was measured by an enzymatic method using "Detamina-GL-E" (trade name, manufactured by Kyowa Medex Co., Ltd.).

The results are shown in Table 3.

Further, FIG. 1 shows the above results for corn hemicellulose (Example 1), and FIG. 2 shows the above results for wheat bran hemicellulose (Example 2). In FIG. 1, ○-○ indicates corn hemicellulose, ●-● indicates control, and in Fig. 2, ○-○ indicates wheat bran hemicellulose, ●-● indicates control.

It can be seen from Table 3, FIG. 1 and FIG. 2 that the substance containing hemicellulose as the main component according to the present invention has an effective inhibitory effect on blood sugar level elevation.

Example 3 Soybean was dipped in water to swell, and peeled by hand to obtain a soybean hull. This soybean hull was treated with a homogenizer and pulverized. The soybean hull powder was dried, extracted with a 0.5N aqueous sodium hydroxide solution, and centrifuged to obtain a supernatant, which was neutralized with acetic acid and then passed. The obtained separated liquid was concentrated and desalted using an ultrafiltration membrane (molecular weight cut off 10,000) to obtain a concentrated liquid. When this concentrated solution was used as it was without being dried, the same test as in the above-mentioned test example was carried out, and it was confirmed that the substances were substantially the same in inhibitory effect on blood sugar level elevation as the substances of Examples 1 and 2.

[Effects of the Invention] As described above, according to the present invention, even if an extract, a concentrate or a dry powder is taken as it is without gelation or the like, a blood sugar level elevation suppressing effect can be effectively obtained. be able to. Therefore, it is extremely easy to eat, easy to handle, and easily ingested in daily life.

[Brief description of drawings]

FIG. 1 is a table showing the blood sugar level elevation inhibitory action of a substance containing corn hemicellulose as a main component obtained in the example of the present invention, and FIG. 2 is a main component of wheat bran hemicellulose obtained in another example of the present invention. 3 is a chart showing the blood sugar level elevation inhibitory action of the substance

Claims (3)

[Claims] 1. A blood glucose comprising hemicellulose as a main component, which is obtained by alkali-extracting the outer skin of cereals or beans, or the residue obtained by removing starches, proteins, lipids, minerals, etc. from the outer skin of cereals or beans. A substance that suppresses price increase. 2. The hull of the cereal according to claim 1, which is a kind selected from corn hull, rice sugar, wheat bran, barley bran, hato bran, oat bran, and rye bran. A substance that suppresses blood sugar elevation. 3. The substance for suppressing an increase in blood glucose level according to claim 1 or 2, wherein the outer coat of the legume is the outer coat of soybean, red bean or pea.