JPH07136676A - Biological method for treating drainage - Google Patents
Biological method for treating drainageInfo
- Publication number
- JPH07136676A JPH07136676A JP30716693A JP30716693A JPH07136676A JP H07136676 A JPH07136676 A JP H07136676A JP 30716693 A JP30716693 A JP 30716693A JP 30716693 A JP30716693 A JP 30716693A JP H07136676 A JPH07136676 A JP H07136676A
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- Japan
- Prior art keywords
- silica
- bacteria
- treatment
- image
- microscope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明はシリカを含有する水中に
おいて養生された有効バクテリアによって排水を処理す
る排水の生物処理方法に関し、詳しくは処理系内で優先
種を占める有効バクテリアの顕微鏡画像と処理水性状の
相関を運転管理指標とする排水の生物処理方法に関す
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for biological treatment of wastewater, in which wastewater is treated with effective bacteria that have been cured in silica-containing water. The present invention relates to a biological treatment method for wastewater, which uses the correlation of water quality as an operation management index.
【０００２】[0002]
【従来の技術】本発明者は先に特願平５−２５０１１６
号において、微生物活性を向上させるためにシリカ溶液
を用いる技術を提案した。2. Description of the Related Art The present inventor has previously filed Japanese Patent Application No. 5-250116.
In the article, a technique using a silica solution to improve microbial activity was proposed.
【０００３】更に本発明者は研究を継続したところ、シ
リカ溶液以外に、人工的に作られた不安定型シリカを含
有する鉱物、可溶性シリカの濃縮された土壌、不安定型
シリカが固定された岩石の粒状化物、粉状化物または成
形物を用いても、活性化効果に寄与することがわかっ
た。Further, the present inventor continued his research and found that, in addition to the silica solution, artificial minerals containing labile silica, soil enriched with soluble silica, and rocks to which labile silica was fixed were found. It was found that the use of granules, powders or molded products also contributes to the activation effect.
【０００４】しかし、これらのシリカを用いた微生物活
性化技術においては、シリカと微生物の挙動との関連が
明らかでないため、客観的な運転管理手法を確立するこ
とが困難であった。However, in these microbial activation techniques using silica, it is difficult to establish an objective operation management method because the relationship between silica and the behavior of microorganisms is not clear.
【０００５】[0005]
【発明が解決しようとする課題】そこで、本発明の目的
は、シリカと微生物の挙動との関連を明らかにして、客
観的な運転管理手法を確立することができる排水の生物
処理方法を提供することにある。Therefore, an object of the present invention is to provide a biological treatment method for wastewater, which can clarify the relation between the behavior of silica and microorganisms and establish an objective operation management method. Especially.
【０００６】[0006]
【課題を解決するための手段】上記目的は、シリカを含
有する水中において養生された有効バクテリアによって
排水を処理する際に、該有効バクテリアの確認手段とし
て顕微鏡を用い、該バクテリアの増殖及び又は変化過程
の画像を得て、該画像と処理水性状の相関を運転管理指
標とすることを特徴とする排水の生物処理方法によって
達成される。Means for Solving the Problems The above-mentioned object is to use a microscope as a means for confirming the effective bacteria when treating the wastewater with the effective bacteria aged in silica-containing water, and to grow and / or change the bacteria. This is achieved by a method for biological treatment of waste water, which comprises obtaining an image of a process and using the correlation between the image and the treated water state as an operation control index.
【０００７】本発明の好ましい態様としては、シリカを
含有する水中において養生された有効バクテリアが、バ
チルス属であることであり、シリカと共にアルカリ土類
金属塩を用いることであり、シリカとして、下記（１）
〜（４）の中から選ばれる少なくとも１種を用いること
である。[0007] In a preferred embodiment of the present invention, the effective bacterium cured in water containing silica is of the genus Bacillus, and an alkaline earth metal salt is used together with silica. 1)
~ It is to use at least one kind selected from (4).
【０００８】（１）人工的に作られた不安定型シリカを
含有する鉱物の粒状化物、粉状化物または成形物 （２）可溶性シリカの濃縮された土壌の粒状化物、粉状
化物または成形物 （３）不安定型シリカが固定された岩石の粒状化物、粉
状化物または成形物 （４）人工的に溶解されたシリカ溶液(1) Granules, powders or moldings of minerals containing labile silica artificially produced (2) Granules, powders or moldings of soil enriched with soluble silica ( 3) Granules, powders or moldings of rock to which unstable silica is fixed (4) Artificially dissolved silica solution
【０００９】以下、本発明について詳細に説明する。The present invention will be described in detail below.
【００１０】本発明において、「シリカを含有する水中
において養生する」というのは、養生池にシリカを外部
から添加してもよいし、予め存在させておいてもよいこ
とを意味し、また養生池は生物処理汚泥、雑排水、自然
水、土壌等のいずれであってもよい。In the present invention, "curing in water containing silica" means that silica may be added to the curing pond from the outside, or may be present in advance. The pond may be biologically treated sludge, gray water, natural water, soil or the like.
【００１１】本発明者はシリカと微生物の増殖や微生物
の挙動との関連について顕微鏡観察を行ったところ、シ
リカを含有する水中においてバクテリアを養生すると、
活性度に優れる有効なバクテリアが優先種を占めるよう
になることを見出した。そしてその有効なバクテリアが
バチルス属であることも見出した。The present inventor carried out a microscopic observation on the relationship between silica and the growth of microorganisms and the behavior of microorganisms. As a result, when bacteria were cured in water containing silica,
It has been found that effective bacteria with excellent activity come to occupy the priority species. We also found that the effective bacterium is of the genus Bacillus.
【００１２】シリカを含有する水中においてバクテリア
を養生した時に、活性度に優れる有効なバクテリア、特
にバチルス属が優先種を占めるようになる理由は、シリ
カあるいはシリカを取り込んだバクテリアの代謝産物が
バクテリアの細胞膜を浸食する作用を呈するため、浸食
を受けやすいバクテリアは死滅し、浸食に対して抵抗力
の強い有効バクテリア、特にバチルス属のみが生き残り
優先種を占めるようになるものと推論される。When bacteria are aged in water containing silica, effective bacteria having excellent activity, particularly Bacillus species, become the predominant species because the metabolites of silica or bacteria incorporating silica are It is inferred that bacteria exhibiting the action of eroding the cell membrane die, and bacteria susceptible to erosion are killed, and only effective bacteria having strong resistance to erosion, particularly Bacillus, survive and occupy the priority species.
【００１３】本発明においては、有効バクテリアの確認
手段として顕微鏡を用いるが、かかる顕微鏡としては、
市販の顕微鏡を用いることもできるし、あるいは位相差
顕微鏡又は微分干渉顕微鏡を用いることもできる。有効
バクテリアの立体画像を得る上では後者の位相差顕微鏡
又は微分干渉顕微鏡を用いることが好ましい。In the present invention, a microscope is used as a means for confirming effective bacteria. As such a microscope,
A commercially available microscope can be used, or a phase contrast microscope or a differential interference microscope can be used. The latter phase contrast microscope or differential interference microscope is preferably used to obtain a stereoscopic image of effective bacteria.
【００１４】位相差顕微鏡としては、例えばオリンパス
社製「ＢＸ４０−ＰＨＤ」、「ＢＸ５０−ＰＨＤ」、
「ＢＨＳ−ＰＣ」、「ＢＨＴ−ＰＣ」）を用いることが
でき、又は微分干渉顕微鏡としては、例えばオリンパス
社製「ＢＸ５０−ＤＩＣ」、「ＢＨＳ−Ｎ」を用いるこ
とができる。As the phase contrast microscope, for example, "BX40-PHD", "BX50-PHD" manufactured by Olympus Corporation,
"BHS-PC", "BHT-PC") can be used, or as a differential interference microscope, for example, "BX50-DIC" or "BHS-N" manufactured by Olympus Corporation can be used.
【００１５】更に、顕微鏡画像を写真撮影するために、
顕微鏡写真撮影装置（例えばオリンパス社製「ＰＭ２
０」、「ＰＭ３０」）用いることが好ましい。またこの
撮影画像をカラーモニター画像表示するために、顕微鏡
カラーカメラ装置（例えばオリンパス社製「ＩＣＤ−７
４０」）を用いることも好ましい。Furthermore, in order to take a picture of a microscope image,
Microscope photography device (for example, "PM2 manufactured by Olympus Corporation"
0 "," PM30 ") are preferably used. Further, in order to display the photographed image on a color monitor image, a microscope color camera device (for example, "ICD-7 manufactured by Olympus Corporation" is used.
40 ") is also preferably used.
【００１６】本発明においては、かかる顕微鏡を用い
て、シリカによって養生されたバクテリアの増殖及び又
は変化過程の画像を得ることができる。In the present invention, such a microscope can be used to obtain an image of the growth and / or transformation process of bacteria cured by silica.
【００１７】即ち、シリカ養生処理の初期にあっては微
生物系に変化は認められないが、養生の進行と共に初期
から存在していた微生物は減少又は消滅し、時間の経過
と共にバチルス捍菌が糸状体を形成し、更に糸状体が消
滅してバチルス捍菌化し、更に養生処理の進行によりバ
チルスは胞子化することが認められた。従って、顕微鏡
によって、シリカ養生により養生前の微生物系が生物循
環を示すバチルス系へと変化する過程の画像を得ること
ができる。That is, in the initial stage of silica curing treatment, no change was observed in the microbial system, but with the progress of curing, the microorganisms existing from the beginning decreased or disappeared, and with the passage of time, Bacillus bacilli were filamentous. It was confirmed that the body formed, the filamentous body disappeared, the Bacillus bacillus formed, and the Bacillus sporulated by the progress of curing treatment. Therefore, it is possible to obtain an image of a process in which a microbial system before curing is transformed into a Bacillus system showing a biological cycle by silica curing by a microscope.
【００１８】また排水の処理における顕微鏡画像におい
て、バチルス捍菌が栄養を消化して増殖し、糸状体を形
成し、栄養の減少と共にバチルスは捍菌となり、更に胞
子化されることが認められた。In the microscopic image of the wastewater treatment, it was confirmed that the Bacillus bacillus digests nutrients and proliferates to form filaments, and the Bacillus becomes bacillus with the decrease of nutrients and further sporulated. .
【００１９】即ち、シリカによって養生されたバクテリ
アの増殖及び又は変化過程の顕微鏡画像と処理水性状に
相関が認められることが確認された。従って、本発明は
かかる相関をベースにして運転管理指標を形成した点に
特徴があり、例えば反応槽内汚泥、好ましくは最終反応
槽内汚泥の顕微鏡画像（好ましくはモニター画像）にバ
チルス胞子やバチルス捍菌が主として認められる場合
は、排水の処理が最終段階まで進んでいることが理解で
き、またバチルス捍菌や糸状菌が認められる場合は残存
有機物の存在を意味しており、その場合には原水負荷
（例えばＢＯＤ負荷）が高いことが考えられ、現象とし
てはエア量不足、菌体不足となり、エア量不足に対して
はエア量増加対策を行い、また菌体不足に対しては種菌
の追加、養生汚泥の追加、返送汚泥量の増加等の対策を
行う。かかる対策は手動で行ってもよいし、自動で行っ
てもよい。本発明においては、上記以外にも種々の相関
をベースにして運転管理指標を形成できる。That is, it was confirmed that there was a correlation between the microscopic image of the growth and / or change process of the bacteria cured by silica and the treated aqueous state. Therefore, the present invention is characterized in that the operation management index is formed based on such a correlation. For example, a microscopic image (preferably a monitor image) of the sludge in the reaction tank, preferably the final sludge in the reaction tank may be Bacillus spores or Bacillus. When bacillus is mainly found, it can be understood that the treatment of wastewater has advanced to the final stage, and when Bacillus bacillus or filamentous fungus is found, it means the presence of residual organic matter. The raw water load (for example, BOD load) is considered to be high. As a phenomenon, the amount of air is insufficient and the cells are insufficient. If the amount of air is insufficient, measures to increase the amount of air are taken. Measures such as addition, curing sludge, and increase in the amount of returned sludge will be taken. Such measures may be taken manually or automatically. In the present invention, the operation management index can be formed based on various correlations other than the above.
【００２０】かかる生物的挙動は水中にシリカが存在す
ることによって生起し、更にアルカリ土類金属イオン、
好ましくはカルシウム塩及びまたはマグネシウム塩の共
存により、かかる生物的挙動が加速されることが認めら
れた。Such biological behavior is caused by the presence of silica in water, and the addition of alkaline earth metal ions,
It has been found that the coexistence of preferably calcium and / or magnesium salts accelerates such biological behavior.
【００２１】又、バチルス系による排水の処理効果にお
いて、従来の活性汚泥法では考えられない極めて高い処
理効果が発現した。これは汚泥中にシリカを共存させる
ことにより生物系を好気性グラム陰性菌から好気性グラ
ム陽性菌であるバチルス系に移行し、更に、アルカリ土
類金属イオンによりバチルスの活性を高め、処理速度を
速めた結果と考えられる。In addition, regarding the effect of treating the waste water by the Bacillus system, an extremely high effect of treatment which cannot be considered by the conventional activated sludge method was exhibited. This is because the coexistence of silica in the sludge shifts the biological system from the aerobic gram-negative bacterium to the aerobic gram-positive bacterium Bacillus, and further enhances the activity of Bacillus by alkaline earth metal ions to increase the treatment rate. It is considered that the result is accelerated.
【００２２】本発明において、シリカは、上記（１）〜
（４）の中から選ばれる少なくとも１種が用いられる。In the present invention, silica is any of the above (1) to
At least one selected from (4) is used.
【００２３】（１）の人工的に作られた不安定型シリカ
を含有する鉱物としては、例えば高炉スラグ等が用いら
れる。（２）の可溶性シリカの濃縮された土壌として
は、腐植土（例えばピートモス）等が用いられる。
（３）の不安定型シリカが固定された岩石としては、ク
リストバル石、あるいは安山岩や流紋岩のような火山性
岩石、堆積岩（例えばケイ藻土）等が用いられる。
（４）の人工的に溶解されたシリカ溶液としては、高炉
スラグ等を硫酸等によって溶解して得られるシリカ溶液
が用いられる。As the mineral containing the artificially produced unstable silica of (1), for example, blast furnace slag or the like is used. As the soil in which the soluble silica is concentrated in (2), humus soil (for example, peat moss) or the like is used.
As the rock to which the unstable silica is fixed in (3), Cristobal stone, volcanic rock such as andesite and rhyolite, sedimentary rock (eg, diatomaceous earth) and the like are used.
As the artificially dissolved silica solution of (4), a silica solution obtained by dissolving blast furnace slag or the like with sulfuric acid or the like is used.
【００２４】本発明の生物処理が適用される排水は、無
希釈し尿、希釈し尿、雑排水、生活排水、各種工場排水
等のいずれであってもよい。Wastewater to which the biological treatment of the present invention is applied may be undiluted urine, diluted urine, gray water, domestic wastewater, various factory wastewater, and the like.
【００２５】[0025]
【実施例】以下、本発明の実施例により、本発明の内容
を更に詳細に説明する。The contents of the present invention will now be described in more detail with reference to the examples of the present invention.
【００２６】実施例１ 食品加工工場の雑排水２．５リットル及び反応槽引き抜
き汚泥５００ｍｌを混合し、ブドウ糖０．１％、食塩
０．１％、ニュートリ・エントブロス０．１％を加え、
更にモノマー・シリカ溶液（ＳｉＯ2６０ｍｇ相当）を
加えて混合する。Example 1 2.5 liters of miscellaneous waste water from a food processing plant and 500 ml of sludge drawn out from a reaction tank were mixed, and 0.1% of glucose, 0.1% of salt and 0.1% of nutritious broth were added,
Further, a monomer-silica solution (corresponding to 60 mg of SiO 2 ) is added and mixed.
【００２７】これに散気管としてガラスボール（Ｇ２）
３本を挿入して２リットル／ｍｉｎの散気を６０分間行
った後、３０分間休止する間欠散気を繰り返し、排水の
処理の進行と槽内生物の養生挙動を調査した。A glass ball (G2) was used as an air diffuser.
After inserting 3 tubes and performing aeration of 2 liters / min for 60 minutes, intermittent aeration with a pause of 30 minutes was repeated to investigate the progress of wastewater treatment and the curing behavior of organisms in the tank.
【００２８】処理の時間経過を表１に示す。またその処
理の時間経過に伴なうバクテリアの養生挙動を示す顕微
鏡画像のスケッチ（４００倍）を図１に示した。Table 1 shows the time course of the treatment. Further, a sketch (400 times) of a microscope image showing the curing behavior of bacteria with the lapse of time of the treatment is shown in FIG.
【００２９】[0029]
【表１】 図１から明らかなように、シリカ養生処理の初期にあっ
ては微生物系に変化は認められないが、養生の進行と共
に初期から存在していた微生物は減少又は消滅し、時間
の経過と共にバチルス捍菌が糸状体を形成し（６時間経
過後）、更に糸状体が消滅してバチルス捍菌化し（２４
時間）、更に養生処理の進行によりバチルスは胞子化す
ることが認められた（４８時間後）。従って、顕微鏡に
よって、シリカ養生により養生前の微生物系が生物循環
を示すバチルス系へと変化する過程の画像を得ることが
できる。[Table 1] As is clear from FIG. 1, in the initial stage of silica curing treatment, no change was observed in the microbial system, but with the progress of curing, the microorganisms existing from the beginning decreased or disappeared, and the Bacillus paste was treated with the passage of time. The fungus forms a filament (after 6 hours), and the filament disappears to transform into Bacillus (24
It was confirmed that Bacillus sporulates as the curing treatment progresses (after 48 hours). Therefore, it is possible to obtain an image of a process in which a microbial system before curing is transformed into a Bacillus system showing a biological cycle by silica curing by a microscope.
【００３０】また排水の処理において、図１の顕微鏡画
像と表１のデータを比較すると、バチルス捍菌が栄養を
消化して増殖し、糸状体を形成し、栄養の減少と共にバ
チルスは捍菌となり、更に胞子化されることが認められ
た。即ち、シリカによって養生されたバクテリアの増殖
及び又は変化過程の顕微鏡画像と処理水性状に相関が認
められることが確認された。In the treatment of waste water, comparing the microscopic image of FIG. 1 with the data of Table 1, the Bacillus bacillus digests nutrients and proliferates to form filaments, and the Bacillus becomes bacillus with a decrease in nutrients. It was confirmed that the spores were further sporulated. That is, it was confirmed that there is a correlation between the microscopic image of the growth and / or change process of the bacteria cured by silica and the treated aqueous state.
【００３１】実施例２ 実施例１で得られた汚泥（４８時間養生）５００ｍｌに
食品加工工場の雑排水２５００ｍｌを加え、更に、モノ
マーシリカ溶液（ＳｉＯ2２０ｍｇ相当）、Ｍｇ2+ （Ｍ
ｇＯ６ｍｇ相当）、Ｃａ2+ （ＣａＯ７ｍｇ相当）を加え
て混合する。Example 2 To 500 ml of the sludge (cured for 48 hours) obtained in Example 1
Add 2500 ml of miscellaneous wastewater from a food processing plant,
Mersilic solution (SiO220mg equivalent), Mg2+ (M
gO6mg equivalent), Ca2+ Add (CaO 7mg equivalent)
And mix.
【００３２】これに散気管としてガラスボール（Ｇ２）
３本を挿入して２リットル／ｍｉｎの散気を６０分間行
った後、３０分間休止する間欠散気を繰り返し、排水の
処理の進行と槽内生物の養生挙動を調査した。A glass ball (G2) was used as an air diffuser.
After inserting 3 tubes and performing aeration of 2 liters / min for 60 minutes, intermittent aeration with a pause of 30 minutes was repeated to investigate the progress of wastewater treatment and the curing behavior of organisms in the tank.
【００３３】処理の時間経過を表２に示す。またその処
理の時間経過に伴なうバクテリアの養生挙動を示す顕微
鏡画像のスケッチ（４００倍）を図２、３に示した。Table 2 shows the time course of the treatment. In addition, sketches (400 times) of microscope images showing the curing behavior of bacteria over time of the treatment are shown in FIGS.
【００３４】[0034]
【表２】 表２及び図２から明らかな通り、バチルス菌の挙動と処
理の進行が合致することがわかる。またシリカと共にＭ
ｇイオン、Ｃａイオンを共存させた場合には、捍菌化→
糸状化→捍菌化→胞子化のサイクルが上昇し、即ち処理
の速度が上昇し、処理効果が極めて高くなることが明ら
かとなった。[Table 2] As is clear from Table 2 and FIG. 2, it can be seen that the behavior of Bacillus and the progress of the treatment match. Also with silica M
In the case of coexistence of g ion and Ca ion, bacteriolysis →
It was revealed that the cycle of filamentation → bacteriolysis → sporification increased, that is, the treatment speed increased, and the treatment effect was extremely high.
【００３５】実施例３ 実施例２において、シリカ溶液に代えて、高炉スラグの
粉末（２００メッシュ）を６ｇ添加した以外は同様に培
養したところ、実施例２と同様の結果が得られた。Example 3 The same results as in Example 2 were obtained when the same culture was carried out as in Example 2 except that 6 g of blast furnace slag powder (200 mesh) was added instead of the silica solution.
【００３６】実施例４ 実施例２において、シリカ溶液に代えて、腐植土（ピー
トモス）粉末を６０ｇ添加した以外は同様に培養したと
ころ、実施例２と同様の結果が得られた。Example 4 The same results as in Example 2 were obtained when the same culture as in Example 2 was carried out except that 60 g of humus soil (peat moss) powder was added instead of the silica solution.
【００３７】実施例５ 実施例２において、シリカ溶液に代えて、クリストバル
石粉末を３０ｇ添加した以外は同様に培養したところ、
実施例２と同様の結果が得られた。Example 5 The same culture as in Example 2 was carried out except that 30 g of cristobalite powder was added instead of the silica solution.
The same result as in Example 2 was obtained.
【００３８】実施例６ 実施例２において、シリカ溶液に代えて、安山岩粉末を
３０ｇ添加した以外は同様に培養したところ、実施例２
と同様の結果が得られた。Example 6 The same culture as in Example 2 was carried out except that 30 g of andesite powder was added instead of the silica solution.
Similar results were obtained.
【００３９】実施例７ 雑排水２リットルにモノマーシリカ５ｐｐｍ、マグネシ
ウム・イオン２ｐｐｍ、カルシウム・イオン２ｐｐｍを
加えて混合し、時計皿で蓋をし、エージングして雑排水
の挙動を調査した。Example 7 5 ppm of monomer silica, 2 ppm of magnesium ion, and 2 ppm of calcium ion were added to 2 liters of sewage, mixed, and covered with a watch glass and aged to investigate the behavior of the sewage.
【００４０】２４時間後には明らかに水の透明度が上昇
し、ＳＳ分は完全に沈降した。After 24 hours, the transparency of water was clearly increased and the SS content was completely settled.
【００４１】１６８時間後には沈降スラッジの圧縮が認
められ、水の輝きが増した。ちなみに並行放置した雑排
水は腐敗が進行して悪臭を放つようになった。After 168 hours, compression of the settled sludge was observed and the brilliance of the water increased. By the way, the miscellaneous wastewater that had been left for a while started to rot and began to give off a bad odor.
【００４２】更に７２０時間後ではスラッジの減少が認
められ、３６００時間後にはスラッジは消滅した。Further, after 720 hours, a decrease in sludge was observed, and after 3600 hours, the sludge disappeared.
【００４３】原雑排水及び３６００時間後の水質は表３
の通りであり、著しい改善が認められた。また水の腐敗
は全く生じなかった。Table 3 shows the raw wastewater and the water quality after 3600 hours.
The results are as shown in the table and significant improvement was observed. Also, no water spoilage occurred.
【００４４】[0044]
【表３】 次いで、市販の位相差顕微鏡（オリンパス社製「ＢＸ４
０−ＰＨＤ」を用いて生物挙動を調べた。又顕微鏡画像
を写真撮影するために、顕微鏡写真撮影装置（オリンパ
ス社製「ＰＭ２０」）用いた。更にこの撮影画像をカラ
ーモニター画像表示するために、顕微鏡カラーカメラ装
置（オリンパス社製「ＩＣＤ−７４０」）を用いた。[Table 3] Then, a commercially available phase contrast microscope ("BX4 manufactured by Olympus Corporation"
0-PHD "was used to investigate the biological behavior. Further, in order to photograph a microscope image, a microscope photograph photographing device (“PM20” manufactured by Olympus Corporation) was used. Further, in order to display the photographed image on a color monitor image, a microscope color camera device ("ICD-740" manufactured by Olympus Corporation) was used.
【００４５】マグネシウム・イオンを添加した場合と添
加しない場合について、１２０分後、２１０分後、３６
０分後、６００分後、１４４０分後、２８８０分後、６
日後（３日後）の位相差顕微鏡写真（倍率はいずれも４
００倍）を各々図４〜図７に示す。With and without addition of magnesium ion, after 120 minutes, 210 minutes, 36
0 minutes, 600 minutes, 1440 minutes, 2880 minutes, 6
Phase contrast micrograph after 3 days (magnification is 4
00 times) are shown in FIGS. 4 to 7, respectively.
【００４６】[0046]
【発明の効果】本発明によれば、シリカと微生物の増殖
や微生物の挙動との関連について顕微鏡観察を行ない、
シリカを含有する水中においてバクテリアを養生する
と、活性度に優れる有効なバクテリアが優先種を占める
ようになることを見出し、そしてその有効なバクテリア
がバチルス属であることも見出し、更にシリカによって
養生されたバクテリアの増殖及び又は変化過程の顕微鏡
画像と処理水性状に相関が認められることを見出し、シ
リカと微生物の挙動との関連が明らかとなり、客観的な
運転管理手法を確立することができた。According to the present invention, the relationship between silica and the growth of microorganisms and the behavior of microorganisms is microscopically observed,
It was found that when bacteria were aged in water containing silica, effective bacteria with excellent activity became the dominant species, and it was also found that the effective bacteria belonged to the genus Bacillus and were further cured by silica. It was found that there was a correlation between the microscopic image of bacterial growth and / or change process and the treated water quality, and the relationship between the behavior of silica and microorganisms was clarified, and an objective operation management method could be established.
【図１】顕微鏡写真スケッチ[Figure 1] Micrograph sketch
【図２】顕微鏡写真スケッチ[Figure 2] Micrograph sketch
【図３】顕微鏡写真スケッチ[Figure 3] Micrograph sketch
【図４】位相差顕微鏡写真[Figure 4] Phase contrast micrograph
【図５】位相差顕微鏡写真[Figure 5] Phase contrast micrograph
【図６】位相差顕微鏡写真FIG. 6 Phase contrast micrograph
【図７】位相差顕微鏡写真[Figure 7] Phase contrast micrograph
Claims (4)
有効バクテリアによって排水を処理する際に、該有効バ
クテリアの確認手段として顕微鏡を用い、該バクテリア
の増殖及び又は変化過程の画像を得て、該画像と処理水
性状の相関を運転管理指標とすることを特徴とする排水
の生物処理方法。1. When treating wastewater with effective bacteria aged in water containing silica, a microscope is used as a means for confirming the effective bacteria, and an image of the growth and / or change process of the bacteria is obtained, A biological treatment method for wastewater, characterized in that a correlation between an image and treated water quality is used as an operation management index.
有効バクテリアが、バチルス属であることを特徴とする
請求項１記載の排水の生物処理方法。2. The method for biological treatment of waste water according to claim 1, wherein the effective bacteria aged in water containing silica are of the genus Bacillus.
ことを特徴とする請求項１又は２記載の排水の生物処理
方法。3. The biological treatment method for wastewater according to claim 1, wherein an alkaline earth metal salt is used together with silica.
ら選ばれる少なくとも１種を用いることを特徴とする請
求項１、２又は３記載の排水の生物処理方法。 （１）人工的に作られた不安定型シリカを含有する鉱物
の粒状化物、粉状化物または成形物 （２）可溶性シリカの濃縮された土壌の粒状化物、粉状
化物または成形物 （３）不安定型シリカが固定された岩石の粒状化物、粉
状化物または成形物 （４）人工的に溶解されたシリカ溶液4. The method for biological treatment of wastewater according to claim 1, wherein at least one selected from the following (1) to (4) is used as silica. (1) Granules, powders or moldings of minerals containing unstable silica artificially made (2) Granules, powders or moldings of soil in which soluble silica is concentrated (3) Anxiety Granular, powdered or molded material of rock with fixed silica fixed (4) Artificially dissolved silica solution
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30716693A JPH07136676A (en) | 1993-11-12 | 1993-11-12 | Biological method for treating drainage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30716693A JPH07136676A (en) | 1993-11-12 | 1993-11-12 | Biological method for treating drainage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07136676A true JPH07136676A (en) | 1995-05-30 |
Family
ID=17965833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP30716693A Pending JPH07136676A (en) | 1993-11-12 | 1993-11-12 | Biological method for treating drainage |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07136676A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0857696A2 (en) * | 1997-02-05 | 1998-08-12 | Haekang Engineering & Construction Co., Ltd. | A system for treating sewage-water and a method for treating sewage-water using the same |
| WO2016167037A1 (en) * | 2015-04-13 | 2016-10-20 | 富士電機株式会社 | Method for treating wastewater, and activator for treating wastewater |
-
1993
- 1993-11-12 JP JP30716693A patent/JPH07136676A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0857696A2 (en) * | 1997-02-05 | 1998-08-12 | Haekang Engineering & Construction Co., Ltd. | A system for treating sewage-water and a method for treating sewage-water using the same |
| EP0857696A3 (en) * | 1997-02-05 | 1999-01-27 | Haekang Engineering & Construction Co., Ltd. | A system for treating sewage-water and a method for treating sewage-water using the same |
| WO2016167037A1 (en) * | 2015-04-13 | 2016-10-20 | 富士電機株式会社 | Method for treating wastewater, and activator for treating wastewater |
| JPWO2016167037A1 (en) * | 2015-04-13 | 2017-04-27 | 富士電機株式会社 | Wastewater treatment method and activator for wastewater treatment |
| CN107428574A (en) * | 2015-04-13 | 2017-12-01 | 富士电机株式会社 | Sewage water treatment method and the activating agent of sewage disposal |
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