JPH069501B2 - Transplanting needle device for bacterial transplantation device - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a transplant needle device used in a device for transplanting bacteria in a cultured prototype petri dish to another petri dish.

<Prior art> When transplanting cultured bacteria to another petri dish, conventionally, most of the work was performed by using a transplant needle, but when the amount becomes large, the burden becomes very heavy and continuous work is difficult. There was a drawback that became. In addition, when performing this moving work using a machine, especially when inoculating the agar surface of the transplant petri dish with bacteria caught from the original petri dish, the position control of the transplant needle is insufficient, and the petri dish for transplant is inoculated at the time of inoculation. There was a drawback that the agar surface was roughened by piercing the agar surface with the transplant needle and moving the needle with the transplanted needle.

By the way, in Japanese Patent Laid-Open No. 58-201976, a pick-up portion provided at the tip of a rotary arm is provided with a fine wire for transplanting a bacterium (transplanting needle) and a distance measuring means for measuring a distance to a medium surface. A configuration is disclosed in which the operations of the pickup unit and the rotary arm are controlled by a computer so that the bacteria can be transferred from the medium in the culture dish to the medium in the test dish without relying on human hands.

However, according to this configuration, the distance measuring means as described above is indispensable in order to properly contact the thin line, that is, the tip of the transplant needle with the culture medium surface, and thus the configuration of the device becomes complicated and the cost also rises. There's a problem. Also, when inoculating the bacteria linearly by contacting the tip of the transplant needle with the medium surface where there are subtle irregularities, the tip of the transplant needle may be uneven on the medium surface even if the distance measuring means as described above is used. Since it is difficult to move them relatively while softly contacting them,
There is a problem that it is difficult to perform linear inoculation without roughening the surface of the medium surface having such irregularities.

<Purpose> The present invention solves the above-mentioned drawbacks of the prior art, allows for the transplantation of bacteria without roughening the agar surface (medium surface), and provides good linear inoculation even if the agar surface has some irregularities. An object of the present invention is to provide an implantable needle device.

<Structure> The present invention is a transplant needle device in a device for transplanting bacteria, which comprises culturing bacteria from a cultured original petri dish with a transplant needle and inoculating this into a petri dish for transplant. Characterize.

That is, the transplant needle is provided with a block-shaped base portion, and the base portion of the transplant needle is stabilized so that the distal end of the transplant needle is slightly tilted from the direct downward direction in a free state. It is rotatably attached to the transplantation device through a pin provided at a position eccentric from the axis. Then, a rotating means for rotating the base portion around the pin so that the tip of the transplant needle faces the direct downward direction, and the base portion can be locked at a predetermined position where the tip of the transplant needle faces the direct downward direction. And a stopper for restricting rotation of the base at the predetermined position.

<Embodiment> FIG. 1 is a block diagram of an implanting needle device according to the present invention, FIG. 2 is an explanatory view of its operation, and FIGS. 3 (A), (B), and (C) are examples of implanting needles. FIG. 4 is a perspective view showing an embodiment of the transplant needle device incorporated in the automatic transplantation device, and FIG. 5 is a flowchart of the transplantation operation by the device of FIG.

First, FIGS. 1 and 2 will be described. The transplant needle 1 is fixed to the base 2, and the whole is rotatably attached to a surrounding wall or the like (not shown) by a pin 3. The position of the pin 3 is provided at a position eccentric from the axis S, so that when the graft needle 1 is free, as shown in FIG. 2, the tip 11 of the graft needle 1 is slightly inclined from the direct downward direction. For example, it is constructed so as to be stable at an angle of about 15 ° to 20 °. On the other hand, a protrusion 21 is provided on the base 2, and the piston 5 from the lock cylinder 4 constituting the rotating means of the present invention is pressed against the protrusion 21. The implantation needle 1 is rotated clockwise by the pushing motion of the piston 5. The stopper 6 stops this rotation at a fixed position. The stopper 6 stops the graft needle 1 at a position where the tip 11 thereof is directed directly downward. That is, the distal end 11 of the transplant needle 1 is locked at the position directly below by the pushing motion of the piston 5 and the stopper 6.
On the other hand, when the piston 5 retracts and the lock is released, the graft needle 1 moves to a position where its tip 11 is slightly tilted from the direction directly below, and stops at that position and becomes stable.

Instead of using the lock cylinder and the piston 5, the stopper 6 and the electromagnet may be used to magnetically attract the base portion 2 of the graft needle 1 when locking.

The tip 11 of the transplant needle 1 has a rounded shape,
I try not to pierce the agar surface with a weak force.
As the graft needle 1, other needles as shown in FIGS. 3 (A), (B) and (C) can be used. (A) Transplant needle 1
Is a nichrome wire with rounded tip 11 or heat-resistant steel, and other electrically resistive material 1a is made of a conductive material 1 such as copper.
It is a transplanted needle that is connected to b and can be sterilized by self-heating. Further, the transplantation needle of (B) is a metal rod having a rounded tip 11 bent. In the transplant needle 1 of (C), the tip 11 is rounded and the middle portion 12 is thin so that the thermal conductivity is small and sterilization can be performed with a small amount of heat during sterilization processing or the like.

Returning to FIG. 1 and FIG. 2 and continuing the description, at the time of transplantation, it is first necessary to catch the bacteria from the cultured original petri dish G. This fishing operation is performed by lowering the whole, that is, the whole including the needle 1, the lock cylinder 4, the piston 5, and the stopper 6, while keeping the needle 1 in the locked state.

The tip of the transplant 1 pierces the agar K surface due to the descent, and the bacteria adhere to the transplant needle 1 from the colonies formed on the agar K surface. Then, by raising the whole, bacteria can be caught while the needle 1 is attached. After fishing, the next step is to inoculate the bacteria. This inoculation is performed by leaving the graft needle 1 in a free state and lowering the whole, including the graft needle 1, the lock cylinder 4, the piston 5, and the stopper 6 as described above. In this case, the degree of the fall is based on the degree that the implantation needle 1 slightly digs into the agar K surface of the petri dish I for implantation. However, the free end of the graft needle 1 is held with its tip 11 slightly tilted from the direction directly below, so that the tip 11 reaches the agar K plane (indicated by a chain double-dashed line in FIG. 2). ), The needle 1 does not pierce the agar K surface by escaping in the direction indicated by the P arrow even if the amount of descent is more than that. Therefore, the agar surface is prevented from being roughened by the needle 1.

FIG. 4 shows an example in which the transplant needle device according to the present invention is incorporated in an automatic transplant device. That is, the rotor 62 is provided on the base 61, and the base 61 itself moves up and down along the elevating shaft 63 in the vertical direction (Z-axis direction). A plurality of graft needles are attached on the circumference of the rotor 62 at regular intervals. And a pin 3 for rotatably supporting the transplant needle 1
Is bearing on the wall of the rotor 62 in which the base 2 is fitted. The rotor 62 is rotated by a predetermined rotation angle by the drive source 65 and the speed reducer 66 to sequentially move the new graft needle 1 to the downward set position. On the other hand, an XY table 71 on which a pair of the cultured petri dish G and the transplanted dish I can be fixedly mounted, and shafts 72, 73 for moving the table 71 in the X-axis direction and the Y-axis direction, A drive source and its transmission mechanism (not shown) are provided. As a result, the XY table 71 is moved, and the standard petri dish G and the transplant petri dish I are transferred.
And are alternately set under the transplant needle 1 to transplant the bacteria. Details of the transplanting operation will be described with reference to FIG.

First, the rotor 62 rotates and the new graft needle 1 is set in the downward position. Next, the XY table 71 is moved so that the position of a predetermined colony of the original petri dish G is below the implantation needle 1. Next, the base 61 itself descends along the elevating shaft 63. As a result, the transplant needle 1 lightly sticks to the position of a predetermined colony. Next, the base 61 rises and the bacteria are picked up by the transplant needle 1. Next, the XY table 71 is moved and the predetermined position of the petri dish I for implantation is set right below the implantation needle 1.
Next, the lock of the graft needle 1 is released, and the graft needle 1 becomes free (FIG. 2). Then, the base 61 descends again, and the transplantation needle 1 comes into contact with a predetermined agar position in the petri dish I for transplantation to inoculate. In this case, in the case of linear inoculation, the XY table 71 is moved by a predetermined amount with the transplant needle 1 inoculated in the agar, and linear inoculation is performed. At this time, even if there is some unevenness on the agar surface in the petri dish I for transplantation, since the tip of the grafting needle 1 is in a free position with its tip slightly inclined from the direction directly below, depending on the unevenness of the agar surface. As a result, the amount of tilt changes, which allows absorption of irregularities and soft contact. As a result, the linear inoculation can be satisfactorily performed without roughening the agar surface. In the case of spot inoculation, the XY table 71 does not move. When the inoculation is finished, the base 61 rises and the transplant needle 1 is locked on. Since transplantation may be performed from each of a plurality of colonies in the original petri dish G, the same petri dish may be transplanted to different positions many times. When the transplantation is performed a predetermined number of times, the transplanting work ends.

<Effect> The present invention is configured as described above, and the transplant needle is set at the set position.
It is possible to switch between the locked state and the free state, and in the free state, the tip of the transplant needle is configured to stabilize at a position slightly tilted from the direction directly below, so inoculate the petri dish for transplant during the transplant work. However, the inconvenience of piercing the agar surface with a needle is eliminated, and good bacterial transplantation can be achieved.

In addition, even if there is some unevenness on the agar surface when performing a linear inoculation, the tip of the transplanted needle will be in a free posture, so the tip of the transplanted needle will change due to the change in the inclination of the agar surface. It will naturally follow the unevenness of. Therefore, the linear inoculation can be satisfactorily performed without roughening the agar surface.

[Brief description of drawings]

FIG. 1 is a configuration diagram of an implanting needle device according to the present invention, FIG. 2 is an operation explanatory diagram thereof, and FIGS. 3 (A), (B), and (C) are diagrams showing examples of implanting needles, respectively. FIG. 4 is a perspective view showing an embodiment of an implanting needle device incorporated in an automatic implanting device, and FIG.
7 is a flowchart of a transplanting operation by the apparatus shown in the figure. 1 ... Implanting needle 2 ... Base part 3 ... Pin 4 ... Rotating means (lock cylinder) 5 ... Piston 6 ... Stopper 11 ... Tip of implanting needle S ... Shaft center G ... Original petri dish I: Petri dish for transplant

Claims (1)

[Claims] 1. A transplant needle device in a device for transplanting bacteria, which comprises culturing bacteria from a cultured original petri dish with a transplant needle and inoculating this into a petri dish for transplant, wherein the transplant needle is fixed. A pin provided at a position eccentric from the axis of the implantation needle so that when the implantation needle is in a free state, the distal end of the implantation needle stabilizes at a position slightly inclined from the direct downward direction. Is rotatably attached to the transplantation device via a rotation means for rotating the base portion around the pin so that the tip end of the transplantation needle faces the direct downward direction, and the tip end of the transplantation needle. A transplant needle device in a bacterial transplantation device, comprising: a stopper that restricts the rotation of the base portion at the predetermined position so that the base portion can be locked at the predetermined position facing right below.