JPH0694676A - Automatic electrophoretic device - Google Patents

Abstract

PURPOSE:To automatically, accurately and rapidly analyze two or more kinds of samples by automatically selecting the analyzing conditions corresponding to the samples to be inspected and automatically selecting and analyzing the data processing alogrithms corresponding to the samples to be inspected. CONSTITUTION:An analyzing unit 1 has a wetting tank, a coating tank, a migration tank, a dyeing tank and a densitometer to send a data signal to a data processing part 7. A sensor 2 detects the kind of a tray storing the sample corresponding to a support before or after wetting and a CPU 3 discriminates the tray signal and transmits said signal to an analyzing condition changeover part 5 and a data processing algorithm changeover part 6 in order to convert the same to the preset corresponding analyzing conditions and data processing algorithm. The changeover part 5 selects specific analyzing conditions to send the same to a unit control part 4 as command data and the control part 4 performs the analysis corresponding to the kind of the sample in the tray. The changeover part 6 selects specific data processing algorithm to send the same to the processing part 7 and the processing part 7 performs the data processing corresponding to the sample.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic electrophoresis apparatus for automatically analyzing the protein fraction value of serum and the like and the protein concentration contained in each fraction in the field of clinical examination.

[0002]

2. Description of the Related Art Samples analyzed by electrophoresis in general hospitals are human serum, urine, recall (cerebrospinal fluid) and the like. In addition, since various test centers request analysis from animal hospitals and the like, in addition to these, the serum of animals such as dogs, cats, rats, and cows may be analyzed. When analyzing these samples, it is desirable to set the analysis conditions according to each sample, perform the analysis, and perform data processing by a data processing algorithm according to each sample type and analysis condition. However, the analyzers currently used are designed on the assumption of human serum analysis, and when analyzing animal serum, analysis conditions and data processing algorithms must be manually switched. Only the payment is made.

[0003]

Therefore, when a sample is analyzed by an electrophoresis method, it is necessary to change the analysis conditions such as the type and amount of chemicals such as buffers and the migration time depending on the type of sample to be measured. Each time the sample was changed, there was a problem that the analysis conditions had to be changed manually or the chemicals had to be replaced. For this reason, in the measurement of a certain type of sample, the analysis may be performed under fixed analysis conditions, and the analysis result may be manually corrected to obtain the final result. Further, in the measurement of a certain kind of sample, even if the densitometry is normally performed, appropriate data may not be obtained because the data processing algorithm is not appropriate.

Further, when the setting of the analysis conditions is manually changed, the continuous operation of the apparatus has to be temporarily interrupted, which causes a time loss. Further, not manually performing the work of exchanging chemicals each time the type of sample changes is troublesome and causes a time loss. Further, when the analysis is performed under a certain analysis condition and the analysis result output on the monitor screen or the like is manually corrected, there is a problem that the correction work is troublesome and time is lost. The present invention is proposed to solve the above-mentioned problems, and an object of the present invention is to provide an automatic electrophoresis apparatus capable of automatically and accurately analyzing a plurality of types of samples.

[0005]

To achieve the above object, the present invention comprises means for wetting a support, means for applying a sample in a sample tray, and means for applying electrophoresis to the support. , A means for dyeing the electrophoresed support, a means for decolorizing the dyed support, a means for photometrically measuring the decolorized support, a data processing means for processing the photometric result, and a data processing means. In an automatic electrophoresis apparatus having means for outputting processed data, means for recognizing the type of sample tray and means for switching analysis conditions in each of the means are provided, and analysis should be performed according to the type of sample tray. This is an automatic electrophoresis device configured to automatically select optimum analysis conditions for a sample and execute analysis. Further, a means for wetting the support, a means for applying the sample in the sample tray, a means for applying electrophoresis to the support, a means for staining the support subjected to the electrophoresis, and a stained support are provided. In an automatic electrophoresis apparatus comprising means for decolorizing, means for photometrically measuring the decolorized support, data processing means for processing photometric results, and means for outputting data processed by the data processing means A means for recognizing the type and a means for switching the data processing algorithm are provided, and the automatic configuration is made so that the optimum data processing algorithm for the sample to be analyzed is automatically selected according to the type of the sample tray and the analysis is executed. This is an electrophoretic device.

[0006]

[Function] Since the type of the sample sample to be analyzed is detected and the analysis condition corresponding to the sample sample is automatically selected and analyzed, it is necessary to manually change the setting of the analysis condition. Accurate and quick analysis can be performed without bothering. In addition, since it is configured to detect the type of sample sample to be analyzed and automatically select and analyze the data processing algorithm according to the sample sample, it is appropriate without correcting the data manually. By processing the data with various algorithms, it is possible to perform accurate and quick analysis.

[0007]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 is a block diagram showing the configuration of a control system for an automatic electrophoresis device according to the present invention. Figure 1
As shown in FIG. 1, the control system for the automatic electrophoretic device according to the present invention includes an analysis unit 1, a sensor 2, a CPU 3, a unit control unit 3, an analysis condition switching unit 5, a data processing algorithm switching unit 6, a data processing unit 7, and an SCR. (screen)
8, PRT9 (printer) is provided.

Of the above, the analysis unit 1 has a wetting, coating, migration tank, dyeing tank, and densitometer. The data signal from the densitometer is sent to the data processing unit 7 and displayed on the SCR 8 in a desired format on the screen. , PRT
A printer output is made from 9. Next, the sensor 2 detects the type of the tray containing the sample corresponding to one of the supports before and after wetting. The detection in this case may be performed either when the tray is transported to the position of the coating means (applicator), during transport of the tray, or in the stored state.

The tray signal obtained by the sensor 2 is discriminated by the CPU 3 and is transmitted to the analysis condition switching unit 5 and the data processing algorithm switching unit 6 respectively for conversion into corresponding preset analysis conditions and data processing algorithms. It has become. The analysis condition switching unit 5 selects a specific analysis condition according to the signal discriminated by the CPU 3 and sends it to the unit control unit 4 as command information. On the other hand, the data processing algorithm switching unit 6 selects a specific data processing algorithm according to the signal determined by the CPU 3 and sends it to the data processing unit 7 as command information.

The CPU 3 is preregistered with analysis conditions, chemicals to be used, and data processing algorithms according to the sample type in the tray. The registered contents are recorded in a master file or a floppy disk and can be read at any time. In this way, the unit controller 4 performs the analysis according to the sample type in the tray, and the data processing unit 7 performs the data processing according to the sample.

FIG. 2A is a diagram showing a specific configuration of the analysis unit 1, and FIG. 2B is a flowchart of the analysis operation performed in the analysis unit 1. Roll-shaped support 10
Is transported to the wetting section 11, cut into a required length by the cutter 12, and then wetted. The support 10 transported to the wet part 11 is automatically transported to the coating position after the infiltration. At the application position, the sample (serum) in the sample tray sent out by the auto feeder 13 is applied by the applicator 14.
Is applied to the adsorption support. The applied adsorption support is conveyed to the two electrophoretic tanks 15 arranged in parallel and sequentially subjected to the electrophoretic treatment.

The support 10 that has been subjected to the electrophoresis treatment is stained and
It is conveyed to the decolorization / drying section 16 and subjected to various treatments such as dyeing, decolorization and drying. Support 10 that has been subjected to treatment such as dyeing
Are automatically conveyed to the densitometer unit 17. In addition, 17a is a sensor. In the densitometer unit 17, the density value of the electrophoretic image is measured at the same time when the support 10 is made transparent, and the analysis result is output. Support 10 after measurement
Are held by the support receiver 18.

As described above, the data signal sent to the data processing unit 7 is subjected to data processing based on the tray type, and the result is output to the PRT 9 and the report 19 is sent.
Is created. Further, the data is output to the floppy disk 20 and stored therein. In this case, the data before the data processing and the data after the data processing can be freely selected and stored. It can be reprocessed with an algorithm.

FIG. 3 is an explanatory view for explaining a method of detecting the type of the sample tray 21, of which FIG. 3A shows
The back side of the sample tray is shown. As shown in the drawing, a hole 22 is formed on the back surface and a magnet 23 is embedded in the hole 22 so that the type can be identified. In the embodiment, the magnets 23 are embedded in 0 and 2 of the holes 22,
No. 1 has no magnet. That is, 3-bit information is written by embedding the magnet 23 at a predetermined position on the back surface of the sample tray, and this information is detected using the Hall element array.

In the example shown in FIG. 3B, the sample tray 21
A hole 22 is formed at a predetermined position on the back surface of the same as in the above-described embodiment, a shielding member insertion hole 24 is formed in the side portion of the sample tray 21, and a shielding member 25 is appropriately inserted therein. 3 bits of information is written, and this information is detected by an optical sensor. As described above, the present embodiment using the hole 22 and the shielding member 25 in combination has an advantage that the sample tray itself can be commonly used since the shielding member 25 can be arbitrarily replaced and used.

The detection of the type of the sample tray is not limited to the above method, and for example, a method of sticking a bar code on the sample tray and detecting this with a bar code reader may be adopted.

FIG. 4 is a diagram for explaining a method of registering analysis conditions and data processing algorithms corresponding to the sample tray. In FIG. 4, only five kinds of conditions A to E are displayed, but this display screen can be scrolled left and right to register eight kinds of conditions. As the analysis conditions, electrophoresis time, type of buffer (buffer solution), buffer comment, staining time, and decolorization time can be set. Further, as the data processing algorithm, eight kinds of algorithms are registered and can be selected according to each subject. The analysis conditions may be such that the type of staining solution is further added so that it can be selectively set according to the sample. In addition to each data processing algorithm,
Data processing comments can be registered.

FIG. 5 is an explanatory diagram showing an example of an analysis condition message text specification showing information transmitted from the CPU 3 to the analysis condition switching unit 5. Various conditions such as electrophoresis time are folded into the analysis conditions of tray A.
Each analysis condition is folded up to.

[0019]

As described above, according to the present invention, when the types of samples to be analyzed are mixed, the time required for switching the analysis conditions is not required, so that the analysis time can be greatly shortened. Further, since no human intervention is required, the efficiency of analysis work can be improved. Furthermore, since the analysis can be performed under the optimum condition for each type of the subject and the data can be processed by the optimum algorithm, the reliability of the data can be significantly improved.

[Brief description of drawings]

FIG. 1 is a block diagram showing a configuration of a control system for an automatic electrophoresis device according to the present invention.

FIG. 2 is a diagram showing a specific configuration of an analysis unit and a flow chart of an analysis operation.

FIG. 3 is a perspective view showing a back surface side of a sample tray.

FIG. 4 is a diagram for explaining a method of registering an analysis condition and a data processing algorithm corresponding to a sample tray.

FIG. 5 is an explanatory diagram showing an example of analysis condition message specifications.

[Explanation of symbols]

 1 analysis unit 2 sensor 3 CPU 4 unit control unit 5 analysis condition switching unit 6 data processing algorithm switching unit 7 data processing unit 8 SCR 9 PRT

Claims (2)

[Claims] 1. A means for wetting a support, a means for applying a sample in a sample tray, a means for applying electrophoresis to the support, a means for staining the support to which the electrophoresis has been applied, and In an automatic electrophoresis apparatus comprising: a means for decolorizing the support, a means for photometrically measuring the decolorized support, a data processing means for processing the photometric result, and a means for outputting the data processed by the data processing means. A means for recognizing the type of sample tray and a means for switching the analysis conditions in each of the above means are provided, and the optimum analysis conditions for the sample to be analyzed are automatically selected according to the type of sample tray to perform the analysis. An automatic electrophoretic device characterized in that 2. A means for wetting a support, a means for applying a sample in a sample tray, a means for applying electrophoresis to this support, a means for staining the support to which electrophoresis has been applied, and In an automatic electrophoresis apparatus comprising: a means for decolorizing the support, a means for photometrically measuring the decolorized support, a data processing means for processing the photometric result, and a means for outputting the data processed by the data processing means. , A means for recognizing the type of sample tray and a means for switching the data processing algorithm are provided so that the optimum data processing algorithm for the sample to be analyzed is automatically selected according to the type of the sample tray to execute the analysis. An automatic electrophoretic device characterized in that