JP2535891B2 - Automatic biochemical analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention is used in the field of biochemical analysis.

The present invention relates to an automatic biochemical analyzer, and more particularly to an automatic biochemical analyzer that can adapt to analytical measurement items with a small amount of sample.

(B) Conventional technology In the conventional multi-item automatic analyzer, the serum amount and the reagent amount are set as the sample amount for each item, and the calibration curve constant is determined by performing the calibration operation to perform the sample analysis. ing.

(C) Problems to be solved by the invention When the analysis request item is determined in this way, the required serum amount (blood sampling amount) is also determined, but this sample amount is generally set for adults. There is.

Therefore, in the case of a patient who does not have enough blood, especially in the case of infants, it is necessary to reduce the number of analysis items or divide the application to the analysis device into two, and execute one under completely different analysis conditions. .

As a result, analytical work is very inefficient, and in some cases, data may not be available to treat sufficient clinical measures.

The object of the present invention is to improve the efficiency of analytical processing of a small amount of sample,
Another object of the present invention is to provide an automatic biochemical analysis device capable of giving clinically sufficient data to a patient with a small amount of sample.

(D) Means for solving the problem The above-mentioned purpose is to analyze each data of the amount of sample used (V0) required for general patients and the amount of sample used (V1) required for minute sample patients for each analytical measurement item. A memory unit that has been written in advance, a data input device for the analysis request sheet that includes the requested measurement items, whether the patient is a general sample or a small sample sample, the input analysis request sheet data, and the read data from the memory section. Based on the above, a sample distributor that distinguishes a general patient from a microsample patient and aspirates the sample volume of the identified one and dispenses it into the reaction tube, and when a microsample item is identified, the ratio of V0 / V1 It is achieved by including a small amount of sample item calculating means for calculating a reagent blank correction value according to the above, and calculating the concentration based on the correction value and a calibration curve constant calculated according to the amount of sample used by a general patient.

(E) Work The amount of serum for general patient sample analysis and the amount of trace sample for each sample to be measured are set in advance for each patient, and the difference between the general sample and the trace sample for each patient is determined. The instruction of the analysis item is sent to the sample distributor by the built-in microcomputer for general data processing, and also has the microcomputer here, and sucks the necessary serum amount collectively according to the analysis required item under the program control, The sample is dispensed to the corresponding reaction tube, and when analyzing a small amount of sample, the corrected sample is output based on the analysis result of the general sample.

(F) Embodiment A preferred embodiment of the present invention will be described with reference to the drawings.

FIG. 1 is an exemplary diagram of the procedure of the calculation means for a small amount of sample item according to the present invention, FIG. 2 is an exemplary diagram of the overall configuration of the present invention, and FIG. 3 is a sample amount used by a general patient according to the present invention and a small amount sample patient It is a table illustration showing the amount of sample used.

In FIG. 2, 10 is a keyboard or an input device for inputting the necessary data described in the analysis request form. At this time, the name, age, etc. are also input, and if an adult can obtain only a small amount of sample, a code to that effect is input. Reference numeral 12 denotes a microcomputer, which has an external storage device such as a floppy disk drive and an internal memory (not shown), and is used for general data processing. A printer 14 prints analysis results and the like.

20 is a sample distributor having a microcomputer and a memory.

According to the application example of the present invention, a table as shown in FIG. 3 is stored which shows the amount of used sample (V ₀ ) of a general patient and the amount of used sample (V ₁ ) of a minute sample patient for each measurement item. The floppy disk is driven to transfer the data from the internal memory of the microcomputer 12 to the internal memory of the sample distributor 20. Further, the measurement items and the like described in the analysis request form are also input by the input device 10, and the necessary data are similarly sent to the internal memory of the sample distributor 20.

The calibration curve constant K for each measurement item is based on the amount of serum or sample used in general patients in advance, that is, the flow cell.
It is calculated from the measured values obtained via 24.

 An example of the subsequent operation will be described with reference to FIG.

First, it is determined whether the target sample belongs to a general patient or a small sample patient by searching the input data regarding the analysis request form. By this and the request item data,
The sample distributor 20 reads the table, sucks the required sample amount from the sample cup 18 of the turntable 16, and dispenses it into the reaction tube 22.

If the sample is from a microsample patient,
The analysis result is corrected by calculation and output from the printer 14 as a report. In the correction calculation example, as shown in FIG. 1, the measured reagent blank Ab is corrected as Ab ′, and the concentration C is α · V ₀ / V ₁
Is required.

That is, for a small amount of sample, the reagent blank correction value Ab
Calculated as ′ = {(V _R + V _H20 + V ₀ ) / (V _R + V _H20 + V ₁ )} · Ab, and correcting density C ′ = α · (V ₀ / V ₁ ) · [K × (A-Ab ′ )]
Is calculated.

Here, K is a calibration curve constant, A is sample absorbance, Ab is a reagent blank value, V _R is the total amount of sample used, V _H20 is the amount of pure water dropped from the sample, and α is a V ₀ / V ₁ theoretical value correction coefficient.

If the sample is found to be from a general patient, the concentration C = K · (A-Ab) is calculated as before,
Printed out.

(G) Effect a. The analytical processing of a small amount of sample is made efficient.

b. Sufficient adjacent bed data for a small sample patient can be obtained.

c. High value (dilution) When re-examining a patient sample, it is possible to reduce the sample amount in advance and analyze with the same effect as dilution, so that not only the results can be obtained quickly, but also the reagent cost can be reduced.

[Brief description of drawings]

FIG. 1 is an exemplary diagram of the procedure of the calculation means for a small amount of sample item according to the present invention, FIG. 2 is an exemplary diagram of the overall configuration of the present invention, and FIG. 3 is a sample amount used by a general patient according to the present invention and a small amount sample patient It is a table illustration showing the amount of sample used. 10 is an input device, 12 is a microcomputer, 14 is a printer, 20 is a sample distributor, 22 is a reaction tube, and 24 is a flow cell.

Claims (1)

(57) [Claims] 1. A memory unit in which data of a required sample amount (V0) required for a general patient and a required sample amount (V1) required for a small amount sample patient for each analytical measurement item are written in advance, and a requested measurement item , A data input device for an analysis request sheet including the distinction between a general patient and a micro sample patient, and a general patient or a micro sample patient based on the input analysis request sheet data and the read data of the memory unit. When a sample dispenser that identifies and aspirates the identified sample amount and dispenses it into the reaction tube and a trace sample item are identified, the reagent blank correction value is calculated from the V0 / V1 ratio and the correction is performed. It is characterized by comprising a calculating means for a small amount of sample item for calculating the concentration based on the value and the calibration curve constant calculated according to the sample amount used by a general patient. Automatic biochemical analyzer.