JPH065760Y2 - Microbial filtration bag - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial field of application> The present invention relates to a bag used for preparing a sample when inspecting microorganisms adhering to a solid matter or a specimen containing a solid matter such as bread.

<Prior art> When a sample to which microorganisms such as bacteria have adhered contains solids, it is not possible to obtain a uniform undiluted test solution as it is, so add sterile physiological saline and shake to wash the sample. It was usual to remove the substance and further dilute it as necessary to make a test water stock solution. In order to remove the solid content from the mixture of the sample and the sterilized physiological saline, there are a method of measuring the supernatant after waiting for the solid content to be precipitated, a method of filtering the mixture with a filter paper, and the like. In order to do this easily, a bag as shown in Fig. 3 proposed in Japanese Utility Model Laid-Open No. 57-36892 is devised. That is, the mixture is put into the larger one of the two divided bags (stock solution chamber) (X) through the opening, and after being filtered by the filtering unit (3) such as filter paper and entering the test chamber (Y), It is transferred from the outlet to another container. (E)
Is a partition by heat sealing, and (F) is a heat sealing portion for preventing solid content from flowing out from the opening of the stock solution chamber. This method was an extremely simple and excellent method because the solid content and the undiluted sample solution were hygienically separated inside the bag, but a separate container is required to move the undiluted sample solution into another container. Therefore, it was not always sufficiently satisfactory.

In addition, when the sample is taken out directly from this bag with a pipette, etc., the position where the filter is formed is in the middle of the bag, and it is necessary to tilt the bag sideways to transfer the test water to the test chamber. There was a problem that water would flow out from the opening.

Further, the water detection chamber is usually formed smaller than the stock solution chamber as shown in FIG. 3, but the partition wall that forms the water detection chamber is
Since the two plastic films were directly heat-sealed, the plastic films were in close contact with each other even when trying to open the water detection chamber, and the opening was difficult and it was difficult to use.

<Problems to be Solved by the Invention> The present invention has been made under the circumstances described above, and like the bag shown in FIG. 3, it is possible to sanitarily remove solid matters inside the bag and to detect water in the water detection chamber. Is easy to open, and a fixed amount of undiluted test solution can be obtained with a pipette without spilling the test solution from the test chamber, so the test solution can be directly transferred from the pipette to the medium without the need for a separate container. It is an object of the present invention to provide a filter bag that can be manufactured and does not cause solid content to be mixed.

<Means for Solving the Problems> In order to achieve this object, the present invention overlaps two rectangular flexible plastic films and arranges three of the overlapping side edges of the two plastic films on three sides. Adhering and leaving the other one as an opening without adhering, and from this opening to the bottom adhesion part facing the opening,
The two edges of the two plastic films are provided with opposing edges in the width direction of a rectangular filtration sheet having a length that is substantially the same as the length from the opening to the bottom adhesive portion and that does not allow solids to pass through. A bag having two chambers of different sizes adhered to each other, the large chamber being the stock solution chamber and the other chamber being the water detection chamber, wherein the filtration sheet is folded in two in the width direction, One of the two edges is adhered to one of the two plastic films, and the other edge opposite the edge is on the same surface as the adhered surface of the two edges of the two plastic films. And a filtration sheet sandwiched between two plastic films at the bottom adhesion portion and adhered to each other.

<Operation> The filter bag of the present invention has the divided two chambers, the larger one being the stock solution chamber (X) and the other as the water detection chamber (Y), and the sample and sterile physiological saline are put in the stock solution chamber (X). Use the test water by pipetting it from the test room (Y). Sample solution and saline solution
When placed in (X) and shaken, the filtration sheet reaches the bottom adhesive portion, so that the sample water naturally enters the test chamber through the filtration sheet without the operation of lying the bag over. After this, this test water can be taken out by a pipette.

<Description of Embodiments> Hereinafter, the present invention will be described with reference to the drawings. Figure 1 of the drawing
(a) and (b) respectively show a front view of an embodiment of the present invention and a sectional view of the bag in an opened state, and FIG. 2 is a front view of another embodiment.

1 (a) (b), the bag of the present invention comprises two rectangular plastic films (1) (2) and a filtration sheet (3). ) Has a rectangular shape with a length substantially equal to the length from the opening of the bag to the bottom adhesive portion, as described later.

In the illustrated example, the two plastic films (1) and (2) are separate plastic films, but of course, one plastic film may be folded into two and the two plastic films may be stacked.

Two plastic films (1) and (2) are adhered to each other on three sides around them, that is, (A1) (A2) (A3). However, as will be described later, in the bottom bonding portion (A3), which faces the opening that is the non-bonded portion, a part (A4) thereof
However, since the filtration sheet (3) is interposed between the two plastic films (1) and (2), the two plastic films (1) and (2) are not in direct contact with each other.

As shown in FIG. 1 (b), the filtration sheet (3) is folded in two, and the opposite edges in the width direction thereof are adhered to the respective plastic films (1) and (2). Filtration Sheet (3)
Is attached to the plastic film (1) (B),
The part adhered to the plastic film (2) is indicated by the symbol (C).

At the intersection (A4) between the filtration sheet (3) and the bottom adhesive portion (A3), the plastics that compose both plastic films (1) and (2) penetrate into the filtration sheet (3) and are folded. The obtained filtration sheets (3) are liquid-tightly adhered to each other, that is, both the plastic films (1) and (2) and the filtration sheet (3) are liquid-tightly integrated.

The example shown in FIG. 2 is the same as the bag shown in FIG. 1 except that a partition wall (D) with a seal is provided. The partition wall (D) is a portion (D1) where the filtration sheet (3) is present and a portion (D) where the filtration sheet (3) is not present.
2), and in (D1) each of the two plastic films (1) and (2) is sealed to the filtration sheet (3), but the folded filtration sheets (3) are joined together. Is not glued. In (D2), the two plastic films (1) and (2) are directly sealed. This partition
(D) is not the case where the test water is taken out with a pipette, but when the test water is outflowed and distributed from the water detection chamber (Y) to other containers as in the conventional method, the solid content flows out from the stock solution chamber (X). The bottom adhesive part (A
It is desirable that it is formed obliquely in the 3) direction.

As a material for the plastic films (1) and (2), a plastic film such as polyethylene, polypropylene or polyester, a laminate of these films, or a film in which a heat sealing agent is applied to the inner surface of these films can be used. As the filtration sheet (3), a microporous sheet (heat permeable on at least one side) that allows liquid to pass through but does not allow solids to pass through can be used. For example, synthetic resin nonwoven fabric, perforated plastic, etc. can be used. it can.

The bag of the present invention is stacked, for example, about 10 pieces, accommodated in another large bag, sterilized by radiation such as γ rays, and shipped. At the time of use, the bag of the present invention is taken out and both plastic films are opened. At this time, however, the two plastic films (1) and (2) do not adhere to each other in the portion where the filtration sheet (3) exists. , Easy to open. Stockroom of bag
A sample such as bread and a sterilized physiological saline solution can be added to (X), but it is difficult to handle the bag if an excessively large amount is added. After this, the opening of the bag is clamped to make it liquid-tight, and the bag is shaken to be sufficiently homogenized, after which the test water is taken out. Clamping and shaking can be performed with a stomacher (shaking, homogenizing device).

<Test Example 1> Polyester (12 μm) / Polyethylene (60 μm)
On the inner surface of a tubular film made of, a band-shaped one-sided heat-sealing non-woven fabric: "Heat Pack" (trade name, manufactured by Nippon Paper Industry Co., Ltd., 18 g / m ² ) and "Compo Pack" (trade name, Asahi Kasei Corporation 20 g / m ² ) manufactured by the above method) was heat-bonded to form partition walls.

Next, by pressing the bottom portion at a higher temperature, the polyethylene was sufficiently melted, penetrated into the inside of the nonwoven fabric, and was firmly heat-sealed to produce two types of bags (Fig. 1).

The 100 inspection bags thus obtained were further integrated and packaged in a large bag made of polyester (12 μm) / polyethylene (40 μm), irradiated with 1.5 Mrad of γ-rays and sterilized to obtain a product of the present invention.

A suitable amount of potato salad and physiological saline were added to the stock solution chamber of the product thus obtained, and the mixture was homogenized with a stomacher manufactured by Seward. I was able to.

Note that the films were not in close contact with each other in the portion where the filtration sheet was present, and it was extremely easy to open.

<Test Example 2> Polyester (12 μm) / Polypropylene (60 μm
A band-shaped one-sided heat-sealing non-woven fabric: “Melfit” (trade name, manufactured by Teijin Ltd., 40 g / m ² ) was heat-bonded to the inner surface of the tubular film composed of m). Next, the bottom portion is sufficiently heated and pressed to bond the bottom portion to obtain the bag shown in FIG.
Similarly, it was γ-ray sterilized.

Then, when potato salad was homogenized with a stomacher as a sample, clear test water was obtained in the test chamber and could be taken out with a pipette.

Note that the films were not in close contact with each other in the portion where the filtration sheet was present, and it was extremely easy to open.

<Effects> As described above, according to the present invention, it is possible to hygienically separate the solid content and the undiluted test water inside the bag, and further, the test water can be taken out with a pipette.

[Brief description of drawings]

1 (a) and (b) of the drawings are a front view of an embodiment of the present invention and a cross-sectional view of the bag in an opened state, and FIG. 2 is a front view of another embodiment, and FIG.
The figure is a front view of a conventional bag. (1) (2) …… Plastic film (3) …… Filtration sheet (A1) (A2) (A3) (A4) …… Adhesive part (B) (C) …… Adhesion of filtration sheet and plastic film Part (D) …… Partition wall (X) …… Stock solution chamber, (Y) …… Water detection chamber

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification number Internal reference number FI technology display location C12M 1/12 (72) Inventor Kazuo Shiraishi 1-5-1 Taito, Taito-ku, Tokyo Metropolitan area stamp printing In-house Examiner, Inc. KONTO Akira (56) Bibliography Showa 57-36892 (JP, U) Showa 56-7513 (JP, U)

Claims (1)

[Scope of utility model registration request] 1. Two flexible plastic films each having a rectangular shape are overlapped, three of the four side edges of the two plastic films which overlap each other are bonded, and the remaining one is an opening without being bonded, and A rectangular filter having a length that is substantially the same as the length from the opening to the bottom adhesive portion and that does not allow solids to pass therethrough from the opening portion to the bottom adhesive portion that faces the opening portion. It is a bag in which the opposite edges in the width direction of the sheet are divided into two chambers of different sizes by adhering to the two plastic films respectively, the large chamber being the stock solution chamber and the other chamber being the water detection chamber. Then, the filtration sheet is folded in two in the width direction, one of the two edges is bonded to one of the two plastic films, and the other edge facing the edge is With the bonded surface of the edge For microbial inspection, the same surface is adhered to the other of the two plastic films, and the filtration sheet is adhered by being sandwiched between the two plastic films at the bottom adhesion portion. Filter bag.