JPH064718Y2 - Microbial filtration bag - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to a bag used for preparing a sample when inspecting microorganisms adhering to solid matter or a specimen containing solid matter such as bread.

<Prior art> When a sample to which microorganisms such as bacteria are attached contains solids, a uniform undiluted test solution cannot be obtained as it is, so sterilized physiological saline is added, and the sample is shaken to wash it and then solidified. It was usual to remove the substance and further dilute it as necessary to make a test water stock solution. In order to remove the solid content from the mixture of the sample and the sterilized physiological saline, there are a method of measuring the supernatant after waiting for the solid content to be precipitated, a method of filtering the mixture with a filter paper, and the like. In order to do this easily, a bag as shown in Fig. 3 proposed in Japanese Utility Model Laid-Open No. 57-36892 is devised. That is, the mixture is put through the opening by the larger one of the two divided bags (stock solution chamber) (X), filtered by the filtering unit (3) such as filter paper, and enters the water detection chamber (Y), It is transferred from the outlet to another container. (E) is a partition by heat sealing, and (F) is a heat sealing portion for preventing solids from flowing out from the opening of the stock solution chamber. This method was an extremely simple and excellent method because the solid content and the undiluted sample solution were hygienically separated inside the bag, but a separate container is required to move the undiluted sample solution into another container. Therefore, it was not always sufficiently satisfactory.

In addition, when the sample is taken out directly from this bag with a pipette, etc., the position where the filter is formed is in the middle of the bag, and it is necessary to tilt the bag sideways to transfer the test water to the test chamber. There was a problem that water would flow out from the opening.

Further, the water detection chamber is usually formed smaller than the stock solution chamber as shown in FIG. 3, but the partition wall that forms the water detection chamber is
Since the two films were directly heat-sealed, the films adhered to each other even when the test chamber was opened, and the openings were difficult to use.

<Problems to be Solved by the Invention> The present invention has been made under the circumstances as described above, and like the bag shown in FIG. 3, it is possible to sanitarily remove solid matters inside the bag and to detect water in the water detection chamber. Is easy to open, and a fixed amount of undiluted test solution can be obtained with a pipette without spilling the test solution from the test chamber, so the test solution can be directly transferred from the pipette to the medium without the need for a separate container. It is an object of the present invention to provide a filter bag that can be manufactured and does not cause solid content to be mixed.

<Means for Solving the Problems> In order to achieve this object, the present invention overlaps two rectangular flexible plastic films and adheres three of the overlapping four side edges of the two films. Then, the remaining one is made an opening without adhering, and liquid is permeated and solid is not permeated from this opening to the bottom adhering portion facing the opening, and the bottom adhering from the opening. The edges of the rectangular filtration sheet having a width substantially the same as the length of the two opposite portions in the width direction are adhered to each of the two films and divided into two chambers of different sizes to form a large chamber. Is a stock solution chamber and the other is a water detection chamber, and the filtration sheet is such that one of the two edges is adhered to one of the two films and is opposed to the other edge. Edge is different from the glued surface of said edge Surface is adhered to the other of the two films, and the filtration sheet is further sandwiched and adhered to the two films at the bottom adhering portion. provide.

<Operation> The filter bag of the present invention has a large chamber of the two divided chambers as the stock solution chamber (X) and the other as the water detection chamber (Y), and the sample and sterile physiological saline are placed in the stock solution chamber (X). Use the test water by pipetting it from the test room (Y). Sample solution and saline solution
When placed in (X) and shaken, the filtration sheet reaches the bottom adhesive portion, so that the sample water naturally enters the test chamber through the filtration sheet without the operation of lying the bag over. After this, this test water can be taken out by a pipette.

<Description of Embodiments> Hereinafter, the present invention will be described with reference to the drawings. Figure 1 of the drawing
(a) and (b) respectively show a front view of an embodiment of the present invention and a sectional view of the bag in an opened state, and FIG. 2 is a front view of another embodiment.

1 (a) (b), the bag of the present invention comprises two rectangular plastic films (1) (2) and a filtration sheet (3). ) Has a rectangular shape with a length substantially equal to the length from the opening of the bag to the bottom adhesive portion, as described later.

In the illustrated example, the two plastic films (1) and (2) are separate plastic films, but of course, one plastic film may be folded into two and the two plastic films may be stacked.

Two plastic films (1) and (2) are adhered to each other on three sides around them, that is, (A1) (A2) (3).
However, as will be described later, in the bottom bonding portion (A3), which faces the opening that is the non-bonded portion, a part (A4) thereof
However, since the filtration sheet (3) is interposed between the two plastic films (1) and (2), the two plastic films (1) and (2) are not in direct contact with each other.

As shown in FIG. 1 (b), the opposite edges in the width direction of the filtration sheet (3) are adhered to the respective plastic films (1) and (2). The part where the filtration sheet (3) is attached to the plastic film (1) is (B), the plastic film
The part bonded to (2) is indicated by the symbol (C). Fig. 1 (b)
As can be seen from the above, the edge of the filtration sheet (3) adhered to the plastic film (1) and the edge of the filtration sheet (3) adhered to the plastic film (2) are different from each other. In addition, different surfaces are bonded to each other. Since the bag is open in Fig. 1 (b),
Although the filtration sheet (3) looks bent, it is not bent in the closed state and has a flat shape.

At the intersection (A4) of the filtration sheet (3) and the bottom adhesive portion (A3), the plastic constituting the plastic films (1) and (2) penetrates into the filtration sheet (3) and The plastic films (1) and (2) and the filtration sheet (3) are liquid-tightly integrated.

The example shown in FIG. 2 is the same as the bag shown in FIG. 1 except that a partition wall (D) with a seal is provided. The partition wall (D) is a portion (D1) where the filtration sheet (3) is present and a portion (D) where the filtration sheet (3) is not present.
2), the filtration sheet (3) and the plastic film (1) are sealed in (D1), but the filtration sheet (3) and the plastic film (2) are not sealed (that is, A partition is provided only in the stock solution chamber (X)).

In (D2), the two plastic films (1) and (2) are directly sealed. At the intersection of (D1) and (C), the plastic film is easy to understand.
(1), the filtration sheet (3), and the plastic film (2) are adhered and integrated in this order, but the plastics of both plastic films (1) and (2) become liquid-tight so that the filtration sheet (3 ) Need not be infiltrated.

This partition wall (D) is not the case where the test water is taken out by a pipette, but when the test water is discharged and distributed from the water test chamber (Y) to other containers as in the conventional method, the solid content is removed from the stock solution chamber (X). It is for preventing the liquid from flowing out, and it is desirable that the liquid is formed in the stock solution chamber (X) at an angle to the direction of the bottom adhesive portion (A3).

As a material for the plastic films (1) and (2), a plastic film such as polyethylene, polypropylene or polyester, a laminate of these films, or a film in which a heat sealing agent is applied to the inner surface of these films can be used. As the filtration sheet (3), a microporous sheet having heat sealability on both sides (a liquid is permeable but a solid content is not) can be used, for example, synthetic resin nonwoven fabric, perforated plastic, etc. can be used. .

The bag of the present invention is stacked, for example, about 10 pieces, accommodated in another large bag, sterilized by radiation such as γ rays, and shipped. At the time of use, the bag of the present invention is taken out and the two plastic films are opened. At this time, in the portion where the filtration sheet (3) is present, the two plastic films (1) and (2) are in close contact with each other. Since it is not there, it can be easily opened by sliding this part. Samples such as bread and sterilized saline can be put in the bag's stock solution chamber (X), but if you put it in too much, the bag will be difficult to handle, so it is preferable to put it to about one-third the depth of the bag. . After this, the opening of the bag is clamped to make it liquid-tight, and the bag is shaken to be sufficiently homogenized, after which the test water is taken out. Clamping and shaking can be performed with a stomacher (shaking, homogenizing insertion).

<Test Example 1> Polyester (12 μm) / Polyethylene (60 μm)
On the inner surface of a tubular film made of, a belt-shaped double-sided heat-sealing non-woven fabric: "BT0706W" (trade name, Teijin Ltd.)
35 g / m ² ) of which the one end was heat-bonded to one of the plastic films of the bag and the other end was opposite to the other plastic film.

Next, the bottom portion was pressed at a higher temperature to form a bottom adhesive portion, and a bag (FIG. 1) was obtained.

The 100 inspection bags thus obtained were further integrated and packaged in a large bag made of polyester (12 μm) / polyethylene (40 μm), irradiated with 1.5 Mrad of γ-rays and sterilized to obtain a product of the present invention.

A suitable amount of potato salad and physiological saline were added to the stock solution chamber of the product thus obtained, and the mixture was homogenized with a stomacher manufactured by Seward. I was able to.

The portion where the filtration sheet was present was wide, and the plastic films were not in close contact with each other, which was extremely easy to open.

<Effect> As described above, according to the present invention, it is possible to sanitarily separate the solid content and the test water stock solution inside the bag, and the test water can be taken out with a pipette.

[Brief description of drawings]

1 (a) and (b) of the drawings are a front view of an embodiment of the present invention and a cross-sectional view of the bag in an opened state, and FIG. 2 is a front view of another embodiment, and FIG.
The figure is a front view of a conventional bag. (1) (2)… Plastic film (3)… Filtration sheet (A1) (A2) (A3) (A4)… Adhesive part (B) (C)… Adhesive part of filtration sheet and plastic film (D) ... Partition (X) ... Undiluted solution room, (Y) ... Water detection room

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification number Internal reference number FI technology display location C12M 1/12 (72) Inventor Kazuo Shiraishi 1-5-1 Taito, Taito-ku, Tokyo Metropolitan area stamp printing In-house Examiner, Inc. KONTO Akira (56) Bibliography Showa 57-36892 (JP, U) Showa 56-7513 (JP, U)

Claims (1)

[Scope of utility model registration request] 1. Two flexible plastic films each having a rectangular shape are overlapped, three of the four side edges of the two plastic films which overlap each other are bonded, and the remaining one is an opening without being bonded, and A rectangular filter having a length that is substantially the same as the length from the opening to the bottom adhesive portion and that does not allow solids to pass therethrough from the opening portion to the bottom adhesive portion that faces the opening portion. It is a bag in which the opposite edges in the width direction of the sheet are divided into two chambers of different sizes by adhering to the two plastic films respectively, the large chamber being the stock solution chamber and the other being the water detection chamber. In the filtration sheet, one of the two edges is bonded to one of the two plastic films, and the other edge facing the edge is a surface of the edge bonded. In terms of different from Is adhered to the other plastic film, further filtration sheet is characterized by being pinched bonded to two plastic films in the bottom bonding portion, filtered bag microbiology.