JPH06256104A - Antibacterial action-sustaining disinfectant - Google Patents

Abstract

PURPOSE:To provide an antibacterial action-sustaining disinfectant having not only a sterilization effect but an antibacterial effect on the sterilized part even after sterilization and capable of maintaining an antibacterial effect especially even against MRSA. CONSTITUTION:A growth inhibitory effect of D-limonene, the main component of citrus peel oil on a bacterium such as colibacillus, Staphylococcus aureus or methicillin-resistant Staphylococcus aureus (MRSA), i.e. an antibacterial function is utilized. An antibacterial action especially even against MRSA is recognized. That is to say, a sterilization effect against bacteria and an antibacterial effect on the sterilized part are recognized by using an antibacterial action-sustaining disinfectant containing a medical germicide and limonene. In addition, in case the D-limonene content is >=0.8wt.% based on the total amount, the antibacterial effect is maintained after sterilization. It is recognized that an antibacterial effect is effective especially even against MRSA more preferably in the case of >=1.0wt.% D-limonene content based on the total amount.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antibacterial and persistent disinfectant which takes advantage of, for example, the property of limonene having the property of inhibiting the growth of bacteria, so-called antibacterial property, and more specifically, the antibacterial property can be maintained for a long time. The present invention relates to an antibacterial persistent disinfectant.

[0002]

2. Description of the Related Art In recent years, when a large amount of third-generation cepham antibiotics such as modacin, adicef, and macerin are administered, the emergence of bacteria that have become resistant to the antibiotics has become a problem. For example, methicillin-resistant Staphylococcus aureus (Methic) induces sepsis or the like in a human body with reduced resistance and has a fatal effect in the worst case.
illin-resistant Staphyloc
Nosocomial infections in hospitals due to occus aureus (hereinafter, abbreviated as MRSA) have become a major social problem. It is clear that further administration of antibiotics is not effective for suppressing the MRSA-induced symptoms. Therefore, as an emergency measure against this MRSA, sterilization treatment such as spraying cresol or the like into the hospital room or wiping indoor equipment such as door knobs and handrails of beds with rubbing alcohol is performed.

By the way, a natural product emanating from a tree
Terpenes have a variety of masking properties to mask offensive odors and various
It is known to have biological properties. These te
Rupen is generally (C_TenH₁₆)_nStructural formula of
Isoprene C_FiveH₈Is the basic unit). Up
Limonet, a type of monoterpene among terpenes
(1-Methyl-4- (1-methyleth
enyl) cyclohexene; C_TenH ₁₆) Is optical
It is a natural product having activity and has a boiling point (BP₇₆₃) 17
5 to 176.5 ° C, specific gravity (d_Four ^20.85) 0.8402
Folding rate (n_D) 1.4744, physical properties such as flash point 45 ° C, and
Insoluble in water and slightly soluble in alcohol
It Of this limonene, D-form is orange peel oil (about 90
wt%), mandarin orange oil (containing about 90 wt%), or
So-called citrus peel oil such as lemon peel oil (containing about 87 wt%)
And is a substance that has passed the FDA test in the United States.
It is also approved as a food additive in Japan. Na
The biological properties of the above terpenes include, for example, killing.
Fungal, insecticidal, expectorant, sedative, diuretic, aromatic or
Insecticidal (this insecticidal property is also called phytoncide)
Etc. Of these, bactericidal properties should be noted.
There are kimonos. For example, Kamiyama et al.
Compared to phenol, lemon peel oil is said to contain 7 wt%
It has been reported to have 5.2 times the bactericidal power (Source: Book
Name "Blue Bucks" The mysterious power of plants = Phitton
"", Published April 20, 1980, published by Kodansha).

[0004]

In spite of the peculiar bactericidal property as described above, limonene has hitherto been used mainly for citrus flavors. For example, generally in the United States since the 1940s, various products such as soaps, detergents, cream lotions, and perfumes have been added to 0.005-1.
It has been used as a fragrance in the concentration range of 0 wt%. This means that it is easier to use industrial products such as alcohol, phenol, cresol, formalin or iodine as a bactericide used in hospitals without using such natural products, that is, In the case of natural products, it was considered that it was difficult to obtain the raw materials and secure the amount thereof, or when the limonene concentration in the composition was extremely high, the citrus odor was strong. By the way, in the case of using the above-mentioned industrial product sterilizing agent, if the portion to be sterilized is wiped with, for example, alcohol, it can be sterilized extremely strongly. However, there is a drawback that the so-called antibacterial property hardly lasts because the bactericidal power is lost after the alcohol is evaporated from the sterilized portion and the bacteria cannot be prevented from growing if the bacteria reattach to the sterilized portion. On the other hand, D-
Limonene is contained in a large amount in citrus peel oil of orange peel and lemon peel, and can be obtained in a relatively large amount and easily by pressing and distilling citrus peel discharged during the production of orange juice and lemon juice. You can

The inventors of the present invention have conducted various studies to solve the above problems of the prior art. As a result, not only the sterilization and disinfection of the sterilized portion but also the antibacterial property of the sterilized and disinfected portion continues for a long time. We conducted diligent research on disinfectants to
It has come to provide an antibacterial persistent disinfectant capable of sustaining antibacterial properties against bacteria, especially MRSA.

[0006]

Means for Solving the Problems Through the experiments, the present inventors have found that D-limonene, which is the main component of citrus peel oil, can be transformed into Escherichia coli, Staphylococcus aureus, or methicillin-resistant Staphylococcus aureus (MR).
It was confirmed that bacteria such as SA) have a property of inhibiting the growth thereof, that is, an antibacterial function. In particular, antibacterial properties were also recognized against MRSA, and the invention that could solve the social problems caused by MRSA was completed. That is, the present inventors have clarified that an antibacterial and persistent disinfectant containing a medical disinfectant and limonene can have bactericidal and antibacterial properties against bacteria in the disinfected portion. Further, the content of limonene in the antibacterial persistent disinfectant is at least 0.
When it is 8 wt% or more, the antibacterial property can be maintained mainly by limonene after sterilization, and more preferably 1.0 wt% or more of the whole, especially MR
It was clarified that it also has an effective antibacterial effect against SA. On the other hand, as the content of limonene is higher, the bactericidal effect of limonene itself can be obtained. However, if the content of limonene is too high, the dispersibility of limonene in a medical disinfectant is poor and not practical, and the citrus odor from the disinfectant causes a rather unpleasant feeling, which makes the limonene practical. It is preferable that the content of the medical bactericide and limonene be relatively easily dispersed, and that the content of the product yields a pleasant citrus scent. Further, when the medical bactericidal agent evaporates at room temperature or body temperature, the medical bactericidal agent evaporates with time from the portion to be sterilized, and this evaporation causes the limonene to be concentrated.

[0007]

The medical bactericidal agent referred to in the present invention includes, for example, ethyl alcohol, iso-propyl alcohol, phenol, cresol, formalin, iodide and the like and their aqueous solutions having a bactericidal effect. Further, more preferably as a medical bactericidal agent according to the present invention,
It is preferable to evaporate the limonene at room temperature or body temperature so that limonene is concentrated over time. Examples of the more preferable medical disinfectant as described above include a general-purpose ethyl alcohol 70 wt% aqueous solution, iso-propyl alcohol (boiling point (under normal pressure) = 97 ° C.), or its aqueous solution (Japanese Pharmacopoeia). As the limonene referred to in the present invention, any of D-form, L-form, and DL-form classified from the aspect of optical activity may be used, but more preferably, a relatively large amount is obtained from mandarin orange peel discharged from a juice factory. It is preferable to use D-limonene which is easily available. The limonene is a so-called non-conjugated terpene hydrocarbon, which has a relatively small affinity for water, alcohol and the like, and a mixture thereof is difficult to uniformly disperse, but the mixture contained in a sealed container is used immediately before use. It is sufficient to use it in the state of being dispersed in a sol by vigorously shaking it. Further, a uniformly solubilized product can be used by adding an appropriate amount of a surfactant to these mixtures. Therefore, when the antibacterial continuous disinfectant according to the present invention is smeared or sprayed on the disinfected area, the disinfected area is first sterilized by the action of the medical disinfectant. After that, even if bacteria are newly attached to the disinfected part, the bacteria are sterilized by the medical disinfectant or the growth of the bacteria is prevented by the action of limonene. Further, when the medical bactericidal agent is one that evaporates at room temperature or body temperature, even if this medical bactericidal agent evaporates over time from the sterilized part, limonene is concentrated by this evaporation, so the final Specifically, a limonene liquid film that inhibits the growth of bacteria and also has a bactericidal action is formed on the disinfected portion.

The present inventors have conducted many researches on the relationship between the efficacy of the antibacterial persistent disinfectant of the present invention and social circumstances, and as a result, the antibacterial persistent disinfectant of the present invention has been widely applied. I found that it can be applied. For example, for disinfecting skin,
Tools, tools, textiles, gauze swallows, disinfection of clothes, disinfectant-impregnated hand towels, disinfection of hospital equipment such as hospitals and nursing homes (to prevent nosocomial infections),
For barber, disinfection in beauty facilities, disinfection in public facilities,
It can be used for disinfecting public amusement parks (eg, pachinko machines, game arcades, etc.). That is, the antibacterial persistent disinfectant of the present invention can be sterilized by a medical disinfectant without fail by impregnating the impregnated material such as paper, cloth or mop and rubbing the disinfected area. After the sterilization, it is possible to prevent the growth and multiplication of bacteria newly attached to the disinfected part, which is a social problem. For example, MRS.
It is extremely effective in preventing the spread of nosocomial infection by A.

[0009]

EXAMPLES In order to clarify the technical contents of the present invention, the present invention will be specifically described by the following representative examples.
In addition, it is needless to say that the following embodiments are merely examples embodying the present invention and do not limit the technical scope of the present invention.

Embodiment 1. The antibacterial continuous disinfectant according to the present example originally has a strong bactericidal action of the medical disinfectant contained therein, and therefore, when it is used as a test sample as it is, bacteria are cultivated in the initial stage of culture. It will die in a rage. Therefore, assuming the situation that the medical sterilizing agent after sterilization was evaporated from the disinfecting portion and limonene remained, the bacteria were cultured using only limonene as the above sample, and the growth thereof was observed. (1) "Test outline" D-limonene was used as the limonene, and the agar plate medium to which this D-limonene was diluted at an arbitrary concentration was added to the agar plate medium to inoculate it, followed by culturing. The lowest arbitrary concentration that could be inhibited was defined as the minimum inhibitory concentration. As an arbitrary concentration of D-limonene used for measurement, 0.01 wt%, 0.1 wt% and 1.0 wt diluted with sterile purified water were used.
%, And three concentrations were used. (2) "Test method" 1) Test strain As a test strain, for example, Escherichia coli (Escherich
is coli IFO 12734), Staphylococcus aureus I
FO 12732), MRSA (Methicilli)
n-resistant Staphylococcu
aureus) was used. 2) Enrichment medium and dilution medium Examples of the enrichment medium and the dilution medium include, for example, Mueller Hinton Broth (Mueller Hinton).
Broth (Difco)) was used. 3) Susceptibility measuring medium As a sensitivity measuring medium, for example, Mueller Hinton Medium (Mueller Hinton Me
dium (Difco)) was used. 4) Preparation of plate for sensitivity measurement D was used as a sample by using dimethyl sulfoxide as a solvent.
After preparing a 20% (wt (solute) / vol (solvent)) solution of limonene simple substance, a 2-fold dilution series solution was further prepared with sterile purified water based on this solution. After being uniformly dissolved, 1/9 amount of each diluted solution was added to the sensitivity measurement medium maintained at about 50 ° C. and mixed sufficiently, and then dispensed into a plurality of petri dishes to solidify each. The plate was used for sensitivity measurement. A plate without a sample was also prepared as a test control sample. 5) Preparation of bacterial solution for inoculation As a bacterial solution for inoculation, each test strain was added to a culture medium for enrichment at 35 ° C.
After culturing for 18 to 20 hours, the medium was used for dilution so that the number of bacteria was adjusted to about 10 ⁶ / ml. 6) Culturing Using a nichrome wire loop (loop inner diameter of about 1 mm) to which the inoculum bacterial solution is adhered, the inoculum bacterial solution is streaked for about 2 cm on a susceptibility measuring plate, and at 35 ° C. for 18 to 20 hours. Cultured. 7) Determination After the above culture, the growth of each test strain was observed, and the lowest arbitrary concentration at which the growth was inhibited was defined as the minimum growth inhibitory concentration for each test strain. 8) Judgment result The judgment result is shown in Table 1 below. As is clear from Table 1, the D-limonene concentration was 0.01 wt% and 0.1%.
When the wt% sample was used, the growth of any of the bacteria could not be prevented and no antibacterial property was observed. However, in the case of 1.0 wt%, the growth of any of the bacteria could be inhibited, and antibacterial properties were recognized. That is, the D-limonene concentration of 1.0 wt% was defined as the minimum inhibitory concentration.

[0011]

[Table 1]

As described above, in order to make the most of the characteristic of D-limonene which becomes the minimum inhibitory concentration when the added amount is 1.0 wt%, the "antibacterial continuous disinfectant" of the present embodiment has the following composition. Prepared. 1-10 wt% D-limonene or an equivalent amount of citrus peel oil corresponding to 1-10 wt% of this D-limonene, 0.2 wt% nonionic surfactant, and general-purpose disinfecting ethyl alcohol (about 70 wt% An aqueous solution was used, and the mixture was mixed with 100% by weight of the total amount), and the mixture was stirred to uniformly disperse and dissolve, thereby obtaining an antibacterial continuous disinfectant having bactericidal properties. Therefore, by using the antibacterial persistent disinfectant containing D-limonene described above, the skin or equipment as the portion to be disinfected can be wiped or contained in fibers such as gauze,
They are sterilized by the disinfecting ethyl alcohol in the antibacterial persistent disinfectant. When sterilized and air-dried as it is, ethyl alcohol for disinfection evaporates from the portion to be disinfected and D-limonene is concentrated over time. As a result, a liquid film of D-limonene having not only antibacterial properties but also bactericidal properties is formed on the skin surface, surfaces of appliances and clothing fibers. In this way, the D-
The liquid film of limonene gradually increases its bactericidal and antibacterial properties against Escherichia coli, Staphylococcus aureus, MRSA, etc. due to concentration.
Moreover, since D-limonene is hard to evaporate at room temperature and remains in the disinfecting area for a long time, growth and growth of the bacteria can be prevented for a long time even if the bacteria adhere. Furthermore, since limonene exerts a moisturizing action on human skin tissue, for example, after wiping the fingers with this antibacterial antiseptic disinfectant and evaporating ethyl alcohol for disinfection, the action of limonene causes the fingers to become smooth. You can give a feeling of comfort.

Reference Example 1. In addition to the sterilization / antibacterial effect of the disinfected area, an application example in which the stain removing effect of the disinfected area is also taken into consideration, and a D-limonene-containing emulsion having the following composition is prepared, In the same manner as in 1, the antibacterial activity against each test bacterial species was observed. "Composition of emulsion containing D-limonene" -Citrus peel oil (containing 90 wt% D-limonene) 40.5 wt% -Emulsifier A (sorbitan fatty acid ester) 7.1 wt% -Emulsifier B (polyoxyethylene sorbitan fatty acid ester) 7. 4 wt% -Emulsifier C (polyoxyethylene nonyl phenyl ester) 4.1 wt% -Base material (purified water = ion-exchanged water) 40.8 wt% -pH adjuster (sodium bicarbonate) 0.1 wt% Total composition 100.0 wt% Preparation of D-Limonene-Containing Emulsion "To 408 g of purified water, 74 g of polyoxyethylene sorbitan fatty acid ester, 71 g of sorbitan fatty acid ester, and 41 g of polyoxyethylene nonylphenyl ester are added, and they are stirred to dissolve uniformly. Next, citrus peel oil (D-limonene 9
405 g (containing 0 wt%), and stir until a viscous and uniform emulsion liquid is obtained. The pH value of this emulsion was adjusted to 6 to 7 with sodium bicarbonate to prepare the D-limonene-containing emulsion (cleansing emulsion).

(1) "Test outline" This D-limonene-containing emulsion was diluted to an arbitrary concentration and added to an agar plate medium except that the above-mentioned D-limonene-containing emulsion was used as a sample instead of D-limonene alone. It is the same as in Example 1 that the minimum growth inhibitory concentration is defined as the lowest arbitrary concentration at which the growth of the bacteria can be inhibited after smearing the bacterial solution for inoculation and culturing. As an arbitrary concentration of the D-limonene-containing emulsion used for the measurement, for E. coli and Staphylococcus aureus, 0.02 wt% and 0.2 wt diluted with sterile purified water were used.
% And 2.0 wt%, and MRSA was diluted with sterile purified water at 0.04 wt% and 0.4 wt%.
Three types of concentrations, wt% and 4.0 wt%, were used. (2) "Test method" In addition, 1) the test strain used, 2) the culture medium for enrichment and the culture medium used for dilution, and 3) the culture medium for sensitivity measurement used were D-limonene simple substance according to Example 1 as a sample. This is the same as when it was used, and as described below, 4) the preparation specifications of the plate for sensitivity measurement are different. 4) Preparation of Plate for Sensitivity Measurement After preparing a 40% (wt (solute) / vol (solvent)) solution of a D-limonene-containing emulsion (sample) using sterile purified water as a solvent, based on this solution Furthermore, a 2-fold dilution series solution was prepared. After dissolving these uniformly, about 50 ℃
After adding 1/9 amount of each diluted solution to the above-mentioned susceptibility-measuring medium and thoroughly mixing, the mixture was dispensed into a plurality of petri dishes, and each was solidified to obtain a susceptibility-measuring plate. Similarly, a plate without a sample was prepared as a test control sample. The 5) preparation specifications of the inoculum bacterial solution, 6) culture specifications, and 7) determination method are also the same as when using D-limonene simple substance according to Example 1 as a sample. 8) Judgment result The judgment result is shown in Table 2 below. As is clear from Table 2, for Escherichia coli and Staphylococcus aureus, the concentration of the emulsion containing D-limonene was 0.02 wt% (D-limonene simplex conversion concentration = 0.008 wt%) and 0.2 wt%.
In the case of (concentration of D-limonene simple substance conversion = 0.08 wt%), the growth of any of the bacteria could not be prevented and no antibacterial property was observed. However, 2.0 wt%
In the case of (concentration of D-limonene simple substance conversion = 0.8 wt%), its growth was able to be inhibited and antibacterial property was recognized. That is, for E. coli and Staphylococcus aureus, 2.
The concentration of 0 wt% D-limonene-containing emulsion (D-limonene simple substance conversion concentration = 0.8 wt%) was defined as the minimum inhibitory concentration.

On the other hand, regarding MRSA, the concentrations of the emulsion containing D-limonene are 0.04 wt% (concentration converted to D-limonene simple substance = 0.016 wt%) and 0.4 wt% (D-limonene).
In the case of limonene simple substance conversion concentration = 0.16 wt%), its growth could not be prevented and no antibacterial property was observed. However, 4.0 wt% (D-limonene simple substance conversion concentration = 1.6
In the case of (wt%), its growth could be inhibited and antibacterial properties were recognized. That is, for MRSA, 4.0
The concentration of the milk emulsion containing D-limonene at wt% (concentration in terms of simple substance of D-limonene = 1.6 wt%) was defined as the minimum inhibitory concentration.

[0016]

[Table 2]

The D-limonene-containing milky lotion of this reference example may be sprayed on the portion to be disinfected and then wiped with a cloth. That is, since the D-limonene-containing emulsion has good transferability and solubility to contaminants in the aqueous micelle, it has a relatively good stain-removing effect, and has sterilization of the disinfected area and antibacterial properties after sterilization. Of course, it is effective both in terms of hygiene and cleaning.

Further, in the above-mentioned reference example, the citrus peel oil concentration in the D-limonene-containing emulsion was set to 40.5 wt%, but as is clear from the test results in Table 2, the above citrus peel oil was used. Even when the addition amount of is about 4.0 wt%, an emulsion having antibacterial properties against MRSA can be obtained. When the citrus peel oil has a relatively low concentration as described above, a small amount of the emulsifier needs to be added in terms of compatibility with the base material (purified water), and the risk of the flammability of D-limonene itself can be reduced. Alternatively, it also has an advantage that the odor peculiar to D-limonene emitted from the product detergent is not tight.

As described above, the antibacterial persistent disinfectant of the above example and the D-limonene-containing emulsion of the above reference example are
Rhythmone is not used as the main use of fragrance, but it is relatively easy and available in large quantities. Citrus peel oil (containing a large amount of D-limonene) has a phytoncide property (kills or attracts insects and fungi originally possessed by plants). It has excellent bactericidal and antibacterial properties, which is a characteristic that does not occur, and safety for the human body.

[0020]

INDUSTRIAL APPLICABILITY The present invention relates to an antibacterial persistent disinfectant, which not only has the sterilizing property and antibacterial property of the disinfected area, but also has a long antibacterial property in the disinfected area after sterilization. It relates to a disinfectant that can last for a period of time. Therefore, the realization of the antibacterial and sustained disinfectant of the present invention can exert a bactericidal and antibacterial effect against bacteria in a wide range from home to hospital, and especially against sterilization against MRSA which has become a social problem in recent years. The antibacterial effect is great.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kazuhide Momoi 2-5115 Kosiga, Wakayama, Wakayama Honshu Kosan Co., Ltd.

Claims (3)

[Claims] 1. An antibacterial persistent disinfectant comprising a medical disinfectant and limonene. 2. The limonene is at least 0.8 of the total.
The antibacterial persistent disinfectant according to claim 1, wherein the antibacterial persistent disinfectant is contained in an amount of at least wt%. 3. The antibacterial continuous disinfectant according to claim 1, wherein the medical bactericidal agent evaporates at room temperature or body temperature.