JPH06233807A - Fecal malodor reducing composition - Google Patents

Abstract

PURPOSE:To reduce the content of ammonia, indole, and p-cresol causing the malodor of the feces of animals and reduce the malodor of the feces by containing low-molecule galactomannan. CONSTITUTION:Only the mannose straight chain of guargum or locust bean gum is enzymatically hydrolyzed with beta-mannose to obtain low-molecule galactomannan. The molecular weight of the galactomannan can be changed when the reaction time of enzyme is changed. The chain length of the mannose straight chain is made to distribute within the range of 30-200 units at 80% or above. The malodor of the feces of animals can be reduced very efficiently, thus contributing to the improvement of the environment very much.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a composition for reducing the malodor of feces of livestock, poultry or pets. More specifically, the present invention relates to a composition for reducing the odor of feces and improving the environment by reducing the contents of ammonia, indole and p-cresol, which are the causes of malodor in feces.

[0002]

2. Description of the Related Art In recent years, the breeding environment has become overcrowded in the livestock industry in order to improve the productivity of livestock such as cattle, pigs and chickens and poultry. Under such circumstances, the bad odor caused by feces excreted by livestock and poultry is a serious environmental problem.

Further, in pets kept in general households, the odor of feces excreted by pets can be reduced due to recent changes in the breeding environment such as diversification of raw materials used for pet food or indoor breeding. It is becoming an important issue.

Under such circumstances, various deodorants and deodorants are currently on the market. These deodorants and deodorants are used in the space where excreted feces and urine are present, and are only expected to have secondary effects.

On the other hand, Japanese Patent Laid-Open No. 63-266 discloses a method of improving the odor by allowing an animal to ingest a fragrance, that is, an essential oil component. However, there is a problem in persistence of efficacy due to volatile components, and there is a problem in palatability of animals.

By the way, it is a well-known fact that ammonia, indole and p-cresol in feces are typical malodorous components of feces. It is known that these compounds are produced by metabolism of amino acids by intestinal bacteria of animals. That is, ammonia is produced by deamination of amino acids, or by hydrolysis of urea produced by metabolism of amino acids. Indole and p-cresol are produced by hydrolyzing tryptophan and aromatic amino acids, respectively (Yoshiyuki Morishita, "Structure and Function of Intestinal Flora," Asakura Shoten, 1990). On the other hand, it is known that there are intestinal bacteria called so-called useful bacteria that do not produce these compounds that are malodorous components.

From the above, by controlling the metabolic activity of enterobacteria that produce the compound in the intestines of animals, the content of the compound in feces is reduced and the malodor of the feces of animals is reduced. Is possible.

However, the present situation is that no composition has yet been found which reduces the malodor of animal feces by changing the metabolic activity of the above-mentioned malodor-producing enterobacteria.

[0009]

DISCLOSURE OF THE INVENTION The present invention is directed to ammonia, indole and p-, which are responsible for the malodor of animal faeces.
It is an object of the present invention to provide a method for reducing the malodor of feces by reducing the cresol content.

[0010]

[Means for Solving the Problems] As a result of intensive studies conducted by the present inventors for the purpose of reducing the malodor of feces by reducing the contents of ammonia, indole and p-cresol in the feces of animals, mannose Chain length is 30
For the first time, it was found that low molecular weight galactomannans distributed in the range of ˜200 units by 80% or more reduced the odor of feces, and completed the present invention.

In the present invention, livestock and poultry mean cows,
It refers to animals that are raised industrially such as pigs and chickens,
Pets refer to animals such as dogs and cats that are raised for personal entertainment. The low-molecular-weight galactomannan that is a product of the present invention is, for example, guar gum, locust bean gum, tara gum, or carob gum that is enzymatically mannose-directed using β-mannanase derived from Aspergillus or Rhizopus. It can be obtained by hydrolyzing only the chain. The galactomannan can change the molecular weight by changing the reaction time of the enzyme, but for the purpose of reducing the malodor of the feces of the present invention, the chain length of the mannose straight chain is 80% or more within the range of 30 to 200 units. It is preferable that the particles are distributed, more preferably 50 to 150 units. The chain length of the mannose linear chain of the present invention refers to the number of mannose that is the main chain of galactomannan bound, and the measuring method is not particularly limited, but for example, decomposed polysaccharides can be treated with water. Dissolved in water and using TOSO 803D type high performance liquid chromatography (HPLC) with water as the mobile phase.
Gel filtration is performed with a 3000 PW column and detection is performed with a suggestive refractometer. At this time, a linear dextrin having a known glucose number (glucose number 30, 100, 200) is measured as an index substance to obtain a graph as shown in FIG. From this, 30 to 200 units can be calculated from the area. When the chain length of mannose is shorter than 30 units, the content of reducing sugars increases, so that not only useful bacteria but also enterobacteria other than useful bacteria capable of producing the compound are assimilated, so that the fecal malodor is reduced. Disappears. On the other hand, when the mannose chain length is 200 units or more, the high-molecular weight does not allow the intestinal useful bacteria to grow, so that the intestinal bacterial flora is not improved, and therefore the present effect cannot be expected.

The product of the present invention may be administered alone to animals or may be used by adding it to feed. further,
Regarding the effective amount for reducing the malodor of feces, the galactomannan is 0.03 to 1.50 g per day.
/ Kg of body weight is preferable, and more preferably 0.08 to
0.83g / kg body weight is good. If the dose is less than 0.03 g / kg body weight, the effect is weak.
If the amount is larger than that, an unfavorable effect such as diarrhea occurs.

A detailed description will be given below with reference to embodiments.

【Example】

Example 1 Citric acid was added to 900 parts of water to adjust the pH to 3.0. To this, 0.2 part of β-mannanase derived from Aspergillus and 100 parts of guar gum powder were added and mixed to obtain 40 parts.
The enzyme was allowed to act for 24 hours at ˜45 ° C. 90 ° C after reaction,
The enzyme was inactivated by heating for 15 minutes. The transparent solution obtained by separating the insoluble matter by filtration was concentrated under reduced pressure (solid content: 20%) and then spray-dried to obtain 65 parts of a white powder of low-molecular-weight galactomannan (this product) Invention).
The water-soluble dietary fiber content according to the enzyme gravimetric method was 80%. As a fixed layer, G3000PW (manufactured by Tosoh Corp.) was used as a column, and the result was measured by high performance liquid chromatography. As a result, 80% or more of the sugar chains of the galactomannan had a mannose chain length within the range of 50 to 150 units. Was included in. At this time, as a standard reagent of sugar chain unit, linear dextrin with a known glucose number (glucose number 50,
100, 150) was used.

In the same manner, the reaction time alone was changed to 48 hours, whereby a short mannose straight-chain galactomannan (80% or more of the chain length of mannose was contained within the range of 5 to 25 units). ) Was prepared (comparative product).

Test Example 1 Fifteen beagle dogs (adult dogs) were divided into three groups, with 5 dogs in each group. A group in which 10 g of the galactomannan (product of the present invention) prepared in Example 1 was added to 250 g of dock food was designated as group A, and 10 g of galactomannan having a short chain length (comparative product) was used.
The added group is B group, and the non-added group is C group,
Each was given once a day (morning) for 10 days. Water was freely available. After completion of the test, feces and urine of each group of dogs were collected, and the contents of ammonia, indole, p-cresol in feces and indica (metabolite of indole), p-cresol in urine were measured and A sensory test for odor was performed by a panelist. At the same time, Clostridium bacteria known as a type of malodorous bacteria as intestinal bacteria, Bifidobacteria as a type of useful bacteria, and the total number of bacteria were measured. The results are shown in Tables 1 to 4.

[0016]

[Table 1]

[0017]

[Table 2]

[0018]

[Table 3]

[0019]

[Table 4]

As is clear from Tables 1 and 2, the A group in which the galactomannan was added to the dock food was the B group and the C group.
The contents of ammonia, indole and p-cresol, which are components of fecal malodor, were reduced as compared with the group. This result was in agreement with the result of the sensory test shown in Table 3. Also,
As is clear from Table 4, the number of Clostridium bacteria, which is one of the malodor producing bacteria, in the enterobacteria decreased, and the number of Bifidobacterium, which is one of the useful bacteria, increased.

Test Example 2 27 broilers (chicken) were divided into 3 groups, each group consisting of 9 animals. The group in which 20 g of the product of the present invention was added to the feed was designated as D group, the group in which 20 g of the comparative product was added was designated as E group, and the group without addition was designated as F group, and fed for 14 days. After the test was completed, the feces of the chickens in each group were collected, and the content of ammonia, indole and p-cresol in the feces was measured, and a sensory test for odor was conducted by 5 panelists. The respective results are shown in Tables 5 and 6.

[0022]

[Table 5]

[0023]

[Table 6]

As is clear from Table 5, the contents of ammonia, indole and p-cresol, which are the malodorous constituents of feces, were reduced in the D group in which the product of the present invention was added to the feed as compared with the E group and the F group. . This result was in agreement with the result of the sensory test shown in Table 6.

As is clear from the above, since the product of the present invention is tasteless and odorless, it is not affected by the palatability of animals, and the malodor of feces of animals can be reduced extremely efficiently.

[0026]

EFFECTS OF THE INVENTION Since the product of the present invention can reduce the bad smell of animal feces extremely efficiently, it greatly contributes to the improvement of the environment.

[Brief description of drawings]

FIG. 1 is a diagram of an elution pattern of gel filtration detected by a suggestive refractometer.

Claims (3)

[Claims] 1. A fecal malodor reducing composition for livestock and poultry, comprising galactomannan having a low molecular weight such that the mannose straight chain has a chain length of 80% or more distributed within a range of 30 to 200 units. . 2. A fecal malodor reducing composition for pets, which comprises a galactomannan having a low molecular weight such that the mannose straight chain has a chain length of 80% or more in the range of 30 to 200 units. 3. The fecal malodor reducing composition according to claim 1, wherein the substance causing the malodor is one or more compounds selected from ammonia, indole and p-cresol.