JPH06227901A - Preparation of specimen using phase-transfer ionic compound composition - Google Patents

Abstract

PURPOSE:To easily encapsulate a specimen in a vessel at a position convenient for observation and prepare an encapsulated specimen suitable for admiration and observation. CONSTITUTION:A specimen is encapsulated with an ionic compound composition exhibiting phase-transition between a gelatinous solid state and a solution state by temperature change. A 1st ionic compound composition having higher phase- transition temperature is solidified in a vessel in the form of gel, a specimen is placed on the gel, a 2nd ionic compound composition having a phase-transition temperature lower than that of the 1st composition is poured into the vessel in liquid state at a temperature lower than the phase-transition temperature of the 1st ionic compound composition to embed the specimen in the composition and the product is cooled to obtain the specimen encapsulated in the gelatinous solid composition.

Description

Detailed Description of the Invention

[0001]

INDUSTRIAL APPLICABILITY The present invention uses an ionic compound composition encapsulant that undergoes a phase transition between a gel-like solid and a solution depending on temperature, and facilitates encapsulation specimens of high appreciation value and convenient for observation. How to create.

[0002]

2. Description of the Related Art Conventionally, in enclosing articles and specimens for observation, appreciation, and preservation, various fixing methods have been used to stop and enclose specimens at desired positions in an enclosure. In the formalin solution most often used for preservation, various kinds of threads, plastic tubes, plastic fixing plates and fixing tools have been used for fixing specimens. However, even if these are used, it is difficult to keep the specimen at a desired position, and in some cases, the specimen may be damaged. Also, if these fixing devices are not used, the specimen will float or sink due to its specific gravity, which is not only inconvenient for observation and viewing,
The exposed part in the air and the part in contact with the vessel wall could be moldy due to lack of formalin, and could damage the specimen.

When encapsulating in a high-molecular compound such as a curable acrylic resin, unsaturated polyester resin, or silicone resin, a fixing tool is also required. After the coagulation, the fixing tool is unnecessary, but it is difficult to remove the fixing tool. If not removed, it was unsuitable for viewing. Further, these resins have a problem that they take time to solidify and are inconvenient to manufacture.

[0004]

DISCLOSURE OF THE INVENTION The present invention relates to a method capable of easily preparing a sample using an ionic compound composition that undergoes a phase transition depending on temperature, which solves the drawbacks of the above-mentioned method for preparing an encapsulated preservation sample. Is. We have
It was found that an ionic compound which is completely different from the above-described formalin solution and the encapsulant such as a polymer compound can be used as the encapsulant, and the application was filed as Japanese Patent Application No. 4-192656. As a result of studying a method of preparing a sample using the ionic compound composition, a new method of preparing a sample that solves the above-mentioned problems of the prior art was found and the present invention was completed. Using the method of the present invention, minerals, toys, ornaments, instrument parts,
Wood products, plastic products, etc., animals, insects, fish,
It is possible to easily produce an encapsulated specimen having a good appearance from specimens of crustaceans, plants, ferns, moss, seaweeds, coelenterates, flowers and the like.

[0005]

That is, the present invention relates to a method of encapsulating a specimen using an ionic compound composition which undergoes a phase transition between a gelled solid and a solution according to the temperature, and which has a higher phase transition temperature. Introducing the ionic compound composition of 1 in a liquid form into a container, cooling the first composition into a gel-like solid, and then arranging a specimen in the container so that the specimen is fixed in position by the gel-like solid, Furthermore, the second ionic compound composition having a lower phase transition temperature is introduced into the container in a liquid state, the specimen is embedded in the composition, and the composition is cooled to form a gel-like solid. It is a method of creating a sample. In the present invention, two kinds of ionic compound compositions, which have a phase transition temperature of 3 ° C. or more, more preferably 7 ° C. or more depending on the composition, are used in combination.

The ionic compound composition which undergoes phase transition in the present invention is a transparent water-soluble composition containing one or several kinds of ammonium compounds, water and, if necessary, one or several kinds of phase transition temperature controlling agents. The content of the ammonium-based compound is 15 to 80%, and the content of the phase transition temperature control agent is 0 to 80% (% by weight, relative to the total of water and the phase transition temperature control agent.
Unless otherwise noted, the same shall apply hereinafter), which is a reversible phase transition between a liquid on the high temperature side and a gel-like solid on the low temperature side due to temperature change in the temperature range of 20 ° C to 95 ° C. The phase transition temperature can be adjusted by changing the composition component ratio. The phase transition is from a viscosity of 200 cps or less when the temperature changes within 3 ° C.
It is preferable that a solid gel phase transition occurs within 3 minutes.

Here, the solid gel means a stainless steel ball (diameter
When a sample (6.4 mm, weight 1.0 g) is placed on the surface of the sample and dropped, the gel state is such that the time required to drop 10 cm from the gel surface is 1 minute or more.

The ammonium compounds capable of reversibly undergoing phase transition by temperature change of the present invention refer to ammonium compounds and amine compounds, and the ammonium compounds include quaternary ammonium compounds, preferably quaternary ammonium compounds containing aliphatic hydrocarbons. Then, one example is trimethyllauryl ammonium dimethyl phosphate, trimethyl stearyl ammonium dimethyl phosphate, etc. The amine compound is preferably an amine salt, and one example is dimethyl lauryl amine malate. These ammonium compounds can be used alone or in combination of several kinds.

As the phase transition temperature controlling agent, monohydric or polyhydric alcohols, dihydric alcohol alkyl ethers,
Dihydric alcohol alkyl ethers and organic acid esters thereof are preferable.

The two kinds of phase-transition ionic compound compositions having different phase-transition temperatures used in the present invention have the kind or quantity ratio of the ammonium type compounds as the constituents, or the kind or quantity ratio of the phase-transition temperature adjusting agent, Alternatively, it is prepared by changing the amount ratio of water. Further, one kind of phase-transition ionic compound composition is added to salts such as sodium chloride, potassium nitrate, sodium nitrite, ammonium acetate, calcium acetate, alkalis such as sodium hydroxide and potassium hydroxide, carbonic acid, acetic acid, citric acid, Acids such as oxalic acid, other water-soluble organic substances,
The phase transition temperature can be adjusted by adding an inorganic substance.

In the encapsulation operation, first, two compositions having different phase transition temperatures are separately melted by heating, and a second composition which undergoes a phase transition at a lower temperature is subjected to a phase transition at a higher temperature. The composition is kept warm at a temperature not higher than the phase transition temperature of the composition of 1.
The first composition, which is melted in a container prepared for exhibition and storage and undergoes a phase transition at a higher temperature, is poured in an amount necessary to leave a space between the container and the specimen, and the whole container is cooled to cool the composition. Allow to gel.

Then, the sample is placed and fixed on the first composition gelled and solidified in the container or so as to partially penetrate into the first composition, and a second composition solution that undergoes a phase transition at a lower temperature is added. Pour the specimen until it is completely filled or fills the container. After that, the container is cooled and the second composition is solidified into a gel, and the lid is covered to obtain a sealed sample.

Here, one of the two kinds of phase change compound compositions is mixed with a dye or a pigment for coloring, or two kinds of phase change compound compositions are colored differently by the same method, or powder or The particles can be mixed into one or both or used in combination to give a function of displaying the background or atmosphere of the enclosed specimen to further improve the effect or interior value of the specimen. For example, it can be used as an aerial background in the sea or with clouds, and can provide a realistic effect of various sports such as ski slopes and rocky places.

[0014]

EXAMPLES The present method will be described in more detail below with reference to examples.

Example-1 and Comparative Example-1 Trimethyllaurylamine dimethyl phosphate 73
%, Solfyt 10% composition was diluted with water to obtain a first composition containing 46% trimethyllaurylamine dimethyl phosphate ester, which resulted in a phase transition temperature of 45 ° C. Also, trimethyllaurylamine dimethyl phosphate ester 37.5
% To obtain a second composition having a phase transition temperature of 38 ° C.

50% of the first composition having a phase transition temperature of 45 ° C.
It was dissolved by heating at ℃ and kept at that temperature. Also, the phase transition temperature 38
The second composition at 0 ° C was dissolved by heating at 43 ° C and the temperature was maintained. Inner 5 cm x 5 cm Depth of 10 cm in an acrylic container, the first composition that undergoes a phase transition at 45 ° C.
Pour 0 ml gently and let it stand until the bubbles come out,
It was cooled and solidified into a gel.

A part of the rat heart fixed with formalin is placed on the gelled solidified composition in the container, and a second composition having a phase transition temperature of 38 ° C. melted at 42 ° C. is placed thereon. The container was gently poured into the container, solidified by cooling, and the container was covered. It was possible to easily stop the sample in the middle of the container, and to prepare a sample in which the sample was placed near the center of the container without using any fixture. Although the container was turned upside down for observation, the specimen was easy to see.

Comparative Example-1 On the other hand, in order to enclose a composition having a phase transition temperature of 38 ° C. alone, it was necessary to fix the sample in the container. Further, in the same manner as in the method of the present invention, a predetermined amount of the above-mentioned composition in a dissolved state was poured into a container in advance, solidified in a cooling gel state, a sample was placed, and the same composition in a dissolved state was further injected. However, the gel-like portion that had been previously injected and solidified was dissolved and the position of the sample was not stable, and the sample at the target fixed position could not be obtained.

Example 2 In the same manner as in Example 1, a first phase change compound composition containing 46% of trimethyllaurylamine dimethyl phosphate and having a phase change temperature of 45 ° C. is prepared. Glycerin (3%) was added to a portion of the mixture to obtain a second composition having a phase transition temperature of 38 ° C.
The first composition having a phase transition temperature of 45 ° C. was dissolved by heating at 48 ° C., and the second composition having a phase transition temperature of 38 ° C. was dissolved by heating at 42 ° C. Rat kidneys fixed with formalin containing 3.6% formaldehyde in the same manner as in Example 1 were encapsulated according to a standard method. A specimen in which the kidney remained in the middle of the container could be easily created and was easy to observe.

[0020]

According to the present invention, a specimen can be easily enclosed in a position in a container where it can be easily observed, and an enclosed specimen suitable for appreciation and observation can be obtained.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor, Koki Suzuki, 3-5-21 Midoridai, Kawanishi-shi (72) Renpei Nagase, 1-chome, Haradamotomachi, Toyonaka City 20-37

Claims (1)

[Claims] 1. A method of encapsulating a sample using an ionic compound composition that undergoes a phase transition between a gelled solid and a solution according to temperature, comprising a first ionic compound composition having a higher phase transition temperature. Introduced in a liquid form into a container, cooling the first composition into a gelled solid, and then arranging a specimen in the container so as to be fixed in position by the gelled solid, and having a lower phase transition temperature. A method for preparing a specimen, which comprises introducing the second ionic compound composition in a liquid form into a container, immersing the specimen in the composition, and cooling the composition to form a gel-like solid.