JPH06192443A - Production of re-formed egg shell membrane - Google Patents

Abstract

PURPOSE:To obtain a water-insoluble membrane useful for treating a wound, etc., without the necessity for the addition of components other than those of the egg shell membrane, such as glycerol, by solubilizing the membrane with thiopropionic acid, forming the solution into a thin layer, and drying this layer. CONSTITUTION:A membrane attached to the inside of an avian egg is mixed with thiopropionic acid and heated to desirably 40-90 deg.C to solubilize the membrane. Although thiopropionic acid exists in two isomeric forms alpha and beta, beta-thiopropionic acid is desirably used. The solution of the solubilized egg shell membrane is applied, cast or sprayed in any desired form to form a thin membrane, and this membrane is dried by heating, in a vacuum or by other means to obtain a re-formed egg shell membrane. The thiopropionic acid slightly remaining in the membrane can be removed by immersing it in an alcoholic solution after drying, withdrawing it from the solution, and drying it.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a method for producing a reshaped eggshell membrane.

[0002]

BACKGROUND OF THE INVENTION Eggshell membranes are known to be useful for wound healing, but they are curved and not suitable for covering a wide flat surface. Therefore, in the case of covering a wide flat surface, as shown in, for example, Japanese Patent Application Laid-Open No. 26560/1990, the eggshell membrane is modified to be hydrophilic by salt and pH adjustment in water and dried, and then the fluorine-based coating is used. Mix with alcohol, acetic acid, glycerin, etc. and apply it to casting paper or film,
Further, a method of drying and reforming into a wide sheet-shaped film is used.

[0003]

However, since the membrane produced by the above-mentioned method uses the eggshell membrane modified to be hydrophilic, it does not become an insoluble membrane in an aqueous medium as it is and is made insoluble. In order to do so, a raw material such as glycerin was indispensable for the production.

It is an object of the present invention to provide a method for producing a reshaped eggshell membrane which can be made into a water-insoluble membrane without containing components other than eggshell membrane components such as glycerin.

[0005]

The method for producing a reshaped eggshell membrane according to the present invention which achieves the above object is characterized in that the eggshell membrane is solubilized with thiopropionic acid and then dried to form a thin layer. And

The details of the present invention will be described below.

In the present invention, the reshaped eggshell membrane refers to the eggshell membrane which is solubilized and then formed into an arbitrary membrane. In the present invention, the eggshell membrane refers to a membrane attached to the inner surface of the eggshell of an avian egg, and a representative example thereof is the eggshell membrane of chicken eggs.

In the present invention, thiopropionic acid means propionic acid having a mercapto group, and isomers include α-type and β-type. In the present invention, any isomer may be used, but it is particularly preferable to use β-type thiopropionic acid (β-thiopropionic acid).

The above-mentioned thiopropionic acid may be anhydrous or may be an aqueous solution. When the aqueous solution is used here, it is desirable to use one having a concentration of 1 mol / liter (denoted as M) or more, more preferably 5 M or more. Thiopropionic acid tends to have stronger mechanical properties in the re-formed eggshell membrane as it approaches the anhydrous state, but in the presence of water, it is lower at a lower temperature than that in the nearly anhydrous state. Moreover, the eggshell membranes tend to be solubilized in a short time. Therefore, the concentration of thiopropionic acid in the case of an aqueous solution may be appropriately adjusted according to the purpose.

In the present invention, solubilizing the eggshell membrane with propionic acid means allowing the eggshell membrane to exist in a solution state in a solution of propionic acid. The proportion for solubilization here is 1 to 20%, preferably 3 to 10% of the eggshell membrane (dry matter weight conversion) based on the weight of thiopropionic anhydride.
It may be mixed at a ratio of about%. The temperature for solubilization may be room temperature, but the temperature of the solution of thiopropionic acid is generally preferably 40 to 90 ° C., since it tends to solubilize faster when appropriately heated. The solubilization time depends on the degree of heating, but it is generally desirable to set the time to about 2 to 50 hours.

The thin layer in the present invention means to form a thin film. Specifically, the eggshell membrane solubilizing solution is applied, spread or sprayed on a mold having a shape to be obtained, to form a thin layer. The film. Incidentally, the thickness of the film does not necessarily have to be uniform, and the thickness may be partially changed according to the use, and the film may be provided with a curved surface or an uneven portion according to the use. Good. If the thickness of the film is too thick, the drying step will take too much time, so it is generally 1 mm or less.

Drying in the present invention means evaporating most of the solvent components in the solubilized liquid of eggshell membranes, and drying may be carried out specifically by means of heating, decompression or the like. When the drying is performed by heating, the eggshell membrane is deteriorated if it is performed at an excessively high temperature, so it is desirable to perform the drying at about 60 to 80 ° C. The reshaped eggshell membrane is manufactured as described above.

An example of the method for producing the reformed eggshell membrane will be described below. For example, the eggshell membranes of chicken eggs are kept in thiopropionic acid with stirring at, for example, 80 ° C. for 48 hours to solubilize the eggshell membranes. Then, after removing the non-dissolved material in the solubilized solution, it is cast on a glass plate and thiopropionic acid is removed by drying to obtain a reshaped eggshell membrane.

Although only a small amount of thiopropionic acid remains in the re-formed eggshell membrane only by the above-mentioned drying method by heating or reduced pressure, when the degree of this residual needs to be substantially close to zero, The following or method may be used. In the case of using alcohol After the drying step is completed, it is immersed in an alcohol solution (the higher the concentration, the easier it is to evaporate later) for 5 to 30 hours, taken out and dried. When only the protein component is taken out When a solubilized solution of the eggshell membrane is produced using thiopropionic acid, an organic solvent such as acetone is added to precipitate the protein component of the eggshell membrane. Then, the precipitate is separated, washed with an organic solvent if necessary, dissolved in water, and the solution is applied to a mold, spread and sprayed to dry. The solubilized liquid of the eggshell membrane includes a liquid in which the eggshell membrane is solubilized with thiopropionic acid, and a liquid in which the components of the eggshell membrane are fractionated and dissolved in another solvent.

[0015]

The reshaped eggshell membrane produced by the method of the present invention
Although it is not clear for any reason why it becomes an insoluble membrane in an aqueous medium, the reshaped eggshell membrane produced by the method of the present invention shows that the eggshell membrane solubilized by thiopropionic acid is accompanied by evaporation of the aqueous medium. , It is presumed that it is strongly connected three-dimensionally again.

[0016]

[Example] Eggshell membrane of eggshell (those that have been thoroughly washed and dried)
20 ml of 5M β-thiopropionic acid aqueous solution was added to 1 g, and the mixture was kept at 80 ° C. for 48 hours with stirring to solubilize the eggshell membrane. The resulting solubilized liquid of eggshell membranes was filtered with a filter to remove non-dissolved substances. 1.5 ml of this filtrate
The glass plate (20 cm
X 50 cm), and the mixture was allowed to stand in a thermostatic chamber at 80 ° C for 24 hours under reduced pressure to dry. Next, the glass plate was taken out from the thermostatic chamber and immersed in methanol for 24 hours to almost completely remove the β-thiopropionic acid that could not be evaporated. Then, the glass plate was taken out from the methanol, the film was peeled from the glass plate, and the film was left at room temperature to evaporate the methanol to obtain a reshaped egg shell film in which the residual thiopropionic acid was substantially zero. . This film had a size of 20 cm × 50 cm, a thickness of 15 μm, and was insoluble in water.

[0017]

INDUSTRIAL APPLICABILITY As described above in connection with the examples, according to the present invention, when the eggshell membrane is reformed, it is insoluble in an aqueous medium and substantially free from the conventional inclusion of glycerin or the like. It is possible to produce a reshaped eggshell membrane consisting only of eggshell membrane components.

Claims (1)

[Claims] 1. A method for producing a reshaped eggshell membrane, which comprises solubilizing the eggshell membrane with thiopropionic acid, forming a thin layer of the eggshell membrane, and drying.