JPH06181696A - Method for promoting growth of animal and powdery pharmaceutical preparation of dead microbial cell microorganism belonging to genus clostridium - Google Patents
Method for promoting growth of animal and powdery pharmaceutical preparation of dead microbial cell microorganism belonging to genus clostridiumInfo
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- JPH06181696A JPH06181696A JP4330508A JP33050892A JPH06181696A JP H06181696 A JPH06181696 A JP H06181696A JP 4330508 A JP4330508 A JP 4330508A JP 33050892 A JP33050892 A JP 33050892A JP H06181696 A JPH06181696 A JP H06181696A
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- Prior art keywords
- clostridium
- cells
- microorganism belonging
- dead
- starch
- Prior art date
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Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は動物の成長促進方法、及
びクロストリジウム属菌死菌体粉末製剤に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for promoting animal growth and a powdered preparation of killed Clostridium spp.
【0002】[0002]
【従来の技術及び発明が解決しようとする課題】家畜、
家禽、養殖水産動物では成長促進を目的に抗生物質など
の抗菌性物質等を飼料に添加して広く利用されている。
しかしながら昨今、抗菌性物質等の畜肉、養殖魚への残
留が度々消費者やマスメディア等から指摘され、国民へ
の安全性の高い食糧供給の観点から抗菌性物質等の使用
の見直しがされつつある。一方、人や家畜等の哺乳動物
の健康維持を目的として、生菌剤が古くから使用されて
きた。この生菌剤の作用は有害菌に対する拮抗作用等に
よるとされ、従って生菌剤は生きた菌が必須と考えられ
ている。2. Description of the Related Art Livestock,
In poultry and aquaculture aquatic animals, antibacterial substances such as antibiotics have been widely added to feeds for the purpose of promoting growth.
However, recently, it has been frequently pointed out by consumers and mass media that antibacterial substances, etc. remain in livestock and cultured fish, and the use of antibacterial substances is being reviewed from the viewpoint of safe food supply to the people. is there. On the other hand, probiotics have been used for a long time for the purpose of maintaining the health of mammals such as humans and livestock. It is said that the action of this probiotic agent is due to an antagonistic action against harmful bacteria and the like. Therefore, it is considered that living bacteria are essential as the probiotic agent.
【0003】生菌剤においては、蛋白変性の問題から温
度が上げられず、強度の強いクランプルやペレットが製
造できない。養鰻飼料のような練餌では、生菌の生産す
る酵素により飼料の劣化が生じる。さらに生菌が芽胞形
成菌の場合、死菌の発生を極力防ぐため芽胞を形成させ
るための処理を必要とするので、収率が悪い。そこで本
発明は、抗菌性物質や生菌剤に代わる安全な成長促進物
質を提供することを目的とする。In the case of a probiotic agent, the temperature cannot be raised due to the problem of protein denaturation, and strong crumples and pellets cannot be produced. In a dry feed such as eel feed, the enzyme produced by viable bacteria causes the feed to deteriorate. Further, when the viable bacterium is a spore-forming bacterium, a treatment for forming a spore is required in order to prevent the generation of dead bacterium as much as possible, so that the yield is poor. Therefore, an object of the present invention is to provide a safe growth-promoting substance that replaces the antibacterial substance and the probiotic agent.
【0004】[0004]
【課題を解決するための手段】本発明は、クロストリジ
ウム属に属する微生物の死菌体を動物に経口投与するこ
とを特徴とする動物の成長促進方法、及びクロストリジ
ウム属に属する微生物の栄養体死菌体と糖類及び/又は
澱粉を必須成分とするクロストリジウム属死菌体粉末製
剤に関する。DISCLOSURE OF THE INVENTION The present invention provides a method for promoting growth of an animal, which comprises orally administering killed cells of a microorganism belonging to the genus Clostridium to an animal, and a vegetative cell killed with microorganisms belonging to the genus Clostridium. The present invention relates to a powdered preparation of killed Clostridium spp. Cells containing the body and sugars and / or starch as essential components.
【0005】クロストリジウム属に属する微生物として
は、例えばクロストリジウム・ブチリカム(Clost
ridium butyricum)が挙げられる。特
に好ましいものとしては、例えばミヤイリ菌が挙げられ
る。ミヤイリ菌は学名クロストリジウム・ブチリカム・
ミヤイリ(Clostridium butyricu
m MIYAIRI)と呼ばれているもので、現在、生
菌剤としてミヤリサン株式会社から市販されており、人
や家畜に長年に亘って投与しても全く副作用の認められ
ないものである。このミヤイリ菌の一種としてクロスト
リジウム・ブチリカム・ミヤイリ 588株(微工研条
寄第2789号:FERM BP−2789)があげら
れる。死菌体を製造するための生菌としては芽胞形成期
のものでも栄養体期のものでも制限はないが、栄養体期
のものが好ましい。生菌の製法はUSP 489273
1に開示されている。Examples of microorganisms belonging to the genus Clostridium include Clostridium butyricum ( Clost
ridium butyricum ). Particularly preferred are, for example, Mycobacterium miyali. The bacterium Miyari is scientific name Clostridium butyricum
Clostridium butyricu
m MIYAIRI), which is currently marketed as a probiotic agent by Miyarisan Co., Ltd. and has no side effects even when administered to humans or livestock for many years. As one kind of this Mycobacterium, there are Clostridium butyricum Miyairi 588 strain (Kikuko Kenjo No. 2789: FERM BP-2789). There is no limitation on the viable bacteria for producing dead cells, whether in the spore formation stage or in the vegetative stage, but the vegetative stage is preferable. Production method of live bacteria is USP 489273
1 is disclosed.
【0006】本発明に用いる死菌体は、生菌体を通常の
方法により、例えば熱処理やホルマリン処理その他の殺
菌剤等を用いた処理方法によって得られる。又、実質的
に死菌体と言えるものであればよく、製剤化の際実質的
に死菌体となるのであれば生菌体を用いてもよい。この
クロストリジウム属に属する微生物の栄養体を製造する
には、菌体の培養法、分離法に特に制限なく例えばクロ
ストリジウム属に属する微生物をブドウ糖加普通ブイヨ
ンなどの適当な培地に接種し、30℃〜40℃で14時
間〜24時間前培養する。この培養が終了した液をスケ
ールアップした前培養と同様な培地に接種し、同様な温
度、時間で、本培養する。本培養終了後、遠心分離機な
ど適当な機器を用いて菌体を集めればよい。培地は、該
菌用であれば特に制限はなく、例えばクロストリジウム
ブチリカム菌用の普通ブイヨン(肉エキス0.8%、
ペプトン1%、塩化ナトリウム0.5%)などのいかな
る培地でも使用できるが、ブドウ糖、酵母エキス、ペプ
トンを含む液体培地が好適である。又、特公昭37−8
300号に開示の方法において、培養時間を短くし(例
えば14〜24時間程度)、芽胞形成前の栄養体の状態
で培養を中断し、菌体を集めてもよい。The dead cells used in the present invention can be obtained by a conventional method such as heat treatment, formalin treatment or other treatment method using a bactericide or the like. Further, it may be substantially dead cells, and live cells may be used as long as it is substantially dead cells during formulation. To produce the trophozoites of microorganisms belonging to the genus Clostridium, the microorganisms belonging to the genus Clostridium are inoculated into a suitable medium such as glucose-containing ordinary broth without any particular limitation on the method of culturing the cells and the method of separation, and the culturing is carried out at 30 ° C or higher. Preculture at 40 ° C. for 14 hours to 24 hours. The liquid after this culturing is inoculated into the same medium as the scale-up pre-culture, and the main culture is performed at the same temperature and time. After completion of the main culture, the cells may be collected using an appropriate device such as a centrifuge. The medium is not particularly limited as long as it is for the bacterium, and for example, ordinary broth for Clostridium butyricum bacterium (meat extract 0.8%,
Although any medium such as peptone 1% and sodium chloride 0.5%) can be used, a liquid medium containing glucose, yeast extract and peptone is preferable. In addition, Japanese Examined Sho 37-8
In the method disclosed in No. 300, the culturing time may be shortened (for example, about 14 to 24 hours), the culturing may be suspended in the state of the vegetative body before spore formation, and the bacterial cells may be collected.
【0007】本発明に用いるクロストリジウム属に属す
る微生物の栄養体死菌末を得るには、培養した栄養体期
の菌体を空気中で濾過することにより、又、それだけで
は不十分な場合にはさらに、酸素の存在する水中にその
濾過物を懸濁すればよい(クロストリジウム属菌は、偏
性嫌気性菌なので、その栄養体は空気に触れるだけで死
滅して栄養体死菌体となる)。なお、栄養体死菌体は栄
養体の生菌を通常の方法により、例えば熱処理やホルマ
リン処理その他の殺菌剤等を用いた処理方法によっても
得られる。実質的に死菌体と言えるものであればよく、
製剤化の際実質的に死菌体となるのであれば生菌体を用
いてもよい。In order to obtain the trophozoite powder of the microorganism belonging to the genus Clostridium used in the present invention, the cultured vegetative cells are filtered in the air, or when it is not enough. Furthermore, it is sufficient to suspend the filtrate in water containing oxygen (Clostridium bacteria are obligate anaerobes, so their vegetative body is killed only by touching the air to become vegetative dead cells). . The trophozoite killed cells can be obtained by a normal method of live vegetative cells, for example, a heat treatment, a formalin treatment, or a treatment method using a bactericide or the like. Anything that can be said to be substantially dead cells,
Live cells may be used as long as they are substantially dead cells during formulation.
【0008】本発明で使用する該死菌体には、死菌体1
g当り菌体数109 個以上、好ましくは109 〜1011
個含まれていればよい。なお、菌体数は、生理食塩水に
菌体を乳化した菌体浮遊液を、血球計算盤にとり顕微鏡
にて計数して求めた値である。本発明における製剤中に
は106 個/g以上、好ましくは106 〜1011個/
g、更に好ましくは106 〜1010個/g程度の生菌を
滅菌した死菌体が含まれていればよい。The dead cells used in the present invention include dead cells 1
The number of bacterial cells per g is 10 9 or more, preferably 10 9 to 10 11.
It only needs to be included. The bacterial cell number is a value obtained by counting a cell suspension containing a bacterial cell emulsified in physiological saline in a hemocytometer with a microscope. In the preparation of the present invention, 10 6 / g or more, preferably 10 6 to 10 11 / g
g, and more preferably about 10 6 to 10 10 cells / g of dead cells sterilized with live cells.
【0009】投与する動物は、ヒト以外の動物であり、
例えば家畜、家禽、養殖水産動物などがあげられる。製
剤としては菌体を培養後殺菌処理し、死菌体のみを分離
濃縮後、乾燥した死菌体原末をそのまま製剤とすること
もでき、又米糠油かす、小麦粉、ブドウ糖、無水ケイ
酸、フスマ等、飼料安全法で許可されている任意の賦形
剤と混合した製剤とすることができる。さらに有効成分
として培養した培養液を、残渣も含めて濃縮乾燥し、殺
菌した死菌体含有物を用いることもできる。製剤の形態
としては例えば散剤、錠剤、ペレット、細粒剤、カプセ
ル剤などがあげられる。錠剤を製造する場合、必要に応
じ賦形剤、崩壊剤、結合剤、滑沢剤等を用いて常法によ
りおこなえばよい。The animals to be administered are non-human animals,
Examples include livestock, poultry, and aquaculture aquatic animals. As the preparation, the bacterial cells are sterilized after culturing, and only the dead bacterial cells are separated and concentrated, and the dried dead bacterial cell bulk powder can be directly used as a preparation, or rice bran oil cake, flour, glucose, silicic acid anhydride, The formulation can be prepared by mixing with any excipients approved by the feed safety law such as bran. Further, a culture broth cultivated as an active ingredient, including the residue, is concentrated and dried, and a sterilized cell-containing material can also be used. Examples of the form of the preparation include powders, tablets, pellets, fine granules, capsules and the like. When a tablet is produced, it may be prepared by a conventional method using an excipient, a disintegrating agent, a binder, a lubricant, etc., if necessary.
【0010】賦形剤としては、例えば、乳糖、D−マン
ニトール、D−ソルビトール、白糖等の糖類、トウモロ
コシデンプン、バレイショデンプン等のデンプン類、リ
ン酸カルシウム、硫酸カルシウム、沈降炭酸カルシウム
等の無機塩類等があげられる。Examples of the excipient include sugars such as lactose, D-mannitol, D-sorbitol and sucrose, starches such as corn starch and potato starch, inorganic salts such as calcium phosphate, calcium sulfate and precipitated calcium carbonate. can give.
【0011】崩壊剤としては、例えば、ヒドロキシプロ
ピルスターチ、カルボキシメチルスターチナトリウム、
部分アルファー化デンプン等のデンプン類、カルボキシ
メチルセルロースカルシウム、カルボキシメチルセルロ
ース、低置換度ヒドロキシプロピルセルロース等のセル
ロース誘導体、ポリビニルピロリドンを架橋構造にし
た、その他合成高分子類等があげられる。Examples of the disintegrant include hydroxypropyl starch, sodium carboxymethyl starch,
Examples thereof include starches such as partially pregelatinized starch, cellulose derivatives such as carboxymethylcellulose calcium, carboxymethylcellulose and low-substituted hydroxypropylcellulose, and other synthetic polymers having polyvinylpyrrolidone in a crosslinked structure.
【0012】結合剤としては、例えば、ポリビニルピロ
リドン、ヒドロキシプロピルセルロース、ヒドロキシプ
ロピルメチルセルロース、ゼラチン、アラビアゴム等の
高分子類等があげられる。滑沢剤としては、例えば、タ
ルク、ロウ類、軽質無水ケイ酸等の天然物由来およびそ
の誘導体等、ステアリン酸、ステアリン酸マグネシウ
ム、ステアリン酸カルシウム、ステアリン酸アルミニウ
ム、ショ糖脂肪酸エステル糖の脂肪酸およびその金属塩
類等があげられる。なお、錠剤には他にマクロゴール等
の高分子化合物が適宜使用される。本発明の製剤を動物
の成長促進のために使用する場合は、通常飼料や飲水に
添加して使用されるので、粉末製剤とする方が好まし
い。この場合製剤中のクロストリジウム属に属する微生
物の死菌体粉末0.00005〜80%、好ましくは
0.0005〜70%、更に好ましくは0.01〜50
%であり、残部は担体である。Examples of the binder include polymers such as polyvinylpyrrolidone, hydroxypropylcellulose, hydroxypropylmethylcellulose, gelatin and gum arabic. Examples of the lubricant include talc, waxes, natural products such as light anhydrous silicic acid and derivatives thereof, stearic acid, magnesium stearate, calcium stearate, aluminum stearate, sucrose fatty acid ester sugar fatty acids and the like. Examples thereof include metal salts. In addition, other high molecular compounds such as macrogol are appropriately used for tablets. When the preparation of the present invention is used for promoting the growth of animals, it is usually added to feed or drinking water, and therefore, it is preferable to use a powder preparation. In this case, the dead cell powder of the microorganism belonging to the genus Clostridium in the preparation is 0.00005 to 80%, preferably 0.0005 to 70%, more preferably 0.01 to 50%.
% And the balance is carrier.
【0013】本発明の製剤において、栄養体死菌体とと
もに糖及び/又は澱粉を併用したものは、成長促進作用
がより高く発現されるので好ましい。即ち、この製剤
は、クロストリジウム属に属する微生物の栄養体死菌体
と糖類及び/又は澱粉を必須成分とするクロストリジウ
ム属死菌体粉末製剤である。この死菌体粉末製剤に用い
る糖は、葡萄糖、果糖、蔗糖、オリゴ糖、乳糖、マンニ
トール、ソルビトール、キシリトール等その他糖なら何
でも良いが、乳糖が好ましい。又、澱粉は、小麦澱粉、
馬鈴薯澱粉、とうもろこし澱粉その他澱粉なら何でも良
いが、とうもろこし澱粉が好ましい。クロストリジウム
属に属する微生物の栄養体死菌末には菌体数が109 個
以上/g、好ましくは109 〜1011個/g程度含まれ
ていれば良い。In the preparation of the present invention, a combination of vegetatively killed bacterial cells with sugar and / or starch is preferred because the growth promoting action is further enhanced. That is, this preparation is a powdered Clostridium cell death preparation containing, as essential components, vegetative cells killed by microorganisms belonging to the genus Clostridium and saccharides and / or starch. The sugar used in the powdered preparation of killed cells may be any sugar such as glucose, fructose, sucrose, oligosaccharide, lactose, mannitol, sorbitol, xylitol, etc., but lactose is preferable. Also, starch is wheat starch,
Any potato starch, corn starch or other starch may be used, but corn starch is preferred. It is sufficient that the vegetative dead bacterial powder of a microorganism belonging to the genus Clostridium contains the number of cells of 10 9 or more, preferably about 10 9 to 10 11 / g.
【0014】本発明のクロストリジウム属菌死菌体粉末
製剤を製造するには、例えば死菌体粉末に糖や澱粉粉末
を加え、単に均一に混合しても、又、湿死菌体に糖や澱
粉を加え乾燥粉砕してもよいが、操作の簡便さからする
と、死菌体に糖および/又は澱粉を加え、水中に懸濁さ
せた後、熱風の入り口温度120〜200℃、好ましく
は130〜170℃、出口温度30〜150℃、好まし
くは50〜100℃で、噴霧乾燥した方がよい。糖や澱
粉の添加量はクロストリジウム属に属する微生物の栄養
死菌体1重量部に対し、糖が0.04〜700重量部、
好ましくは0.1〜70重量部、澱粉が0.2〜700
重量部、好ましくは0.4〜70重量部程度である。な
お、噴霧乾燥する場合、炭酸カルシウムなどの水難溶性
の生理的に許容される無機物を添加すると操作が容易と
なる。この場合無機物の添加量は上記の必須成分の総量
に対し0.005〜0.5部、好ましくは0.01〜
0.2部、さらに好ましくは0.01〜0.1部であ
る。In order to produce the Clostridium spp. Killed cell powder preparation of the present invention, for example, sugar or starch powder may be added to the killed cell powder and simply mixed, or the wet killed cells may be mixed with sugar or starch. Although starch may be added and dried and ground, in view of easiness of operation, after adding sugar and / or starch to dead cells and suspending them in water, the inlet temperature of hot air is 120 to 200 ° C., preferably 130. It is better to carry out spray drying at ˜170 ° C., outlet temperature 30˜150 ° C., preferably 50˜100 ° C. The amount of sugar or starch added is 0.04 to 700 parts by weight of sugar with respect to 1 part by weight of dead microbial cells of a microorganism belonging to the genus Clostridium.
Preferably 0.1 to 70 parts by weight, starch is 0.2 to 700
Parts by weight, preferably about 0.4 to 70 parts by weight. In the case of spray drying, the operation becomes easy by adding a poorly water-soluble physiologically acceptable inorganic substance such as calcium carbonate. In this case, the amount of the inorganic substance added is 0.005 to 0.5 part, preferably 0.01 to 0.5, based on the total amount of the above essential components.
0.2 parts, and more preferably 0.01 to 0.1 parts.
【0015】上記方法により得られるクロストリジウム
属菌死菌体粉末製剤の各成分の割合は、クロストリジウ
ム属に属する微生物の栄養体死菌体0.05〜80%、
好ましくは0.5〜70%、さらに好ましくは5〜50
%、糖1〜50%、好ましくは3〜40%、さらに好ま
しくは5〜30%、澱粉15〜90%、好ましくは25
〜80%、さらに好ましくは35〜75%程度であり、
製剤1g当り、栄養体の死菌体が109 個以上、好まし
くは109 〜1011個、さらに好ましくは10 9 〜10
10個含まれていればよい。又無機物を使用している場
合、製剤中のその割合は0.5〜50%、好ましくは1
〜20%、さらに好ましくは1〜10%程度である。Clostridium obtained by the above method
The ratio of each component of the powdered formulation of killed genus bacteria is
0.05-80% of dead vegetative cells of microorganisms belonging to the genus Mumu,
Preferably 0.5-70%, more preferably 5-50
%, Sugar 1-50%, preferably 3-40%, more preferably
5 to 30%, starch 15 to 90%, preferably 25
-80%, more preferably about 35-75%,
10 g of dead microbial cells per 1 g of the preparation9More than one, preferred
Kuha 109-1011More preferably 10 9-10
TenIt only needs to be included. When using inorganic materials
In that case, its proportion in the formulation is 0.5 to 50%, preferably 1
-20%, more preferably 1-10%.
【0016】上記の粉末製剤にはさらに賦形剤粉末を添
加してもよい。賦形剤としては、脱脂米糠、大豆粉、フ
スマ、もみがら粉、炭酸カルシウム、糖、澱粉、ビール
酵母、小麦粉等、飼料安全法で認可されている任意の賦
形剤があげられる。これらの賦形剤は1種のみならず2
種以上併用してもよい。その添加割合は上記の粉末製剤
1重量部当り0.01〜1000重量部、好ましくは
0.1〜800重量部さらに好ましくは1〜500重量
部程度である。この賦形剤入粉末製剤中の死菌体量は、
製剤1g当り菌体数106 個以上、好ましくは106 〜
1011個、さらに好ましくは106 〜1010個程度であ
る。An excipient powder may be further added to the above powder formulation. Examples of the excipient include defatted rice bran, soybean flour, bran, chaff, calcium carbonate, sugar, starch, brewer's yeast, wheat flour, and any other excipient approved by the Feed Safety Law. Not only one of these excipients, but two
You may use together 1 or more types. The addition ratio is 0.01 to 1000 parts by weight, preferably 0.1 to 800 parts by weight, and more preferably 1 to 500 parts by weight, per 1 part by weight of the above powder preparation. The amount of dead cells in this excipient-containing powder formulation is
The number of bacterial cells per gram of the preparation is 10 6 or more, preferably 10 6 to
The number is about 10 11 , more preferably about 10 6 to 10 10 .
【0017】賦形剤入粉末製剤中の各成分の割合を述べ
ると、クロストリジウム属に属する微生物の栄養体死菌
体0.00005〜80%、好ましくは0.0005〜
70%、さらに好ましくは0.01〜25%、糖0.0
01〜50%、好ましくは0.003〜40%、さらに
好ましくは0.01〜15%、澱粉0.015〜90
%、好ましくは0.03〜80%、さらに好ましくは
0.05〜40%、賦形剤1〜99.9%、好ましくは
10〜99.8%、さらに好ましくは50〜99.7
%、程度である。又、上記粉末製剤として無機物を添加
した噴霧乾燥製剤を使用した場合、その無機物の割合は
0.0005〜33%、好ましくは0.001〜15
%、さらに好ましくは0.002〜5%程度がよい。The proportion of each component in the powdered preparation containing excipients will be described. 0.00005-80%, preferably 0.0005-5%, of dead microbial cells of microorganisms belonging to the genus Clostridium.
70%, more preferably 0.01 to 25%, sugar 0.0
01 to 50%, preferably 0.003 to 40%, more preferably 0.01 to 15%, starch 0.015 to 90
%, Preferably 0.03-80%, more preferably 0.05-40%, excipient 1-99.9%, preferably 10-99.8%, more preferably 50-99.7.
%, The degree. When a spray-dried preparation containing an inorganic substance is used as the powder formulation, the proportion of the inorganic substance is 0.0005 to 33%, preferably 0.001 to 15%.
%, And more preferably about 0.002 to 5%.
【0018】なお、賦形剤として糖、澱粉、又は無機物
を使用した場合、製剤中の各成分の割合は、賦形剤と乾
燥粉末中の成分の総量として糖0.001〜99.9
%、好ましくは0.003〜99.8%、さらに好まし
くは0.01〜99.7%、澱粉0.015〜99.9
%、好ましくは0.03〜99.8%、さらに好ましく
は0.05〜99.7%、無機物0.0005〜99.
9%、好ましくは0.001〜99.8%、さらに好ま
しくは0.002〜99.7%程度である。When sugar, starch, or an inorganic substance is used as the excipient, the ratio of each component in the preparation is 0.001 to 99.9 as the total amount of the components in the excipient and the dry powder.
%, Preferably 0.003 to 99.8%, more preferably 0.01 to 99.7%, starch 0.015 to 99.9.
%, Preferably 0.03 to 99.8%, more preferably 0.05 to 99.7%, and inorganic substances 0.0005 to 99.%.
It is about 9%, preferably 0.001 to 99.8%, and more preferably about 0.002 to 99.7%.
【0019】本発明の製剤には更に、流動性改善剤、固
結防止剤、飛散防止剤等の助剤を添加してもよい。本発
明の製剤は、そのまま人、家畜等の動物に投与してもよ
いが、通常、家畜、家禽又は養殖水産動物等の動物飼料
に添加して、又は飲水に懸濁して投与される。投与は必
要のある限りおこなわれ、通常、1ヵ月−数年間にわた
る。投与する動物としては、例えば、牛、豚、馬、羊等
の家畜、ブロイラー、採卵鶏、うずら、カモ、アヒル、
キジ、七面鳥等の家禽、ハマチ、マダイ、フグ、マグ
ロ、ヒラメ、シマアジ、マアジ、サケ、コイ、ウナギ、
ニジマス、アユ、エビ類(クルマエビ、ボタンエビ、イ
セエビ、ロブスター、ブラックタイガー等)、カニ類
(タラバガニ、ズワイガニ、ワタリガニ、ケガニ等)等
の養殖水産動物があげられる。The formulation of the present invention may further contain auxiliaries such as a fluidity improver, anticaking agent and anti-scattering agent. The preparation of the present invention may be directly administered to animals such as humans and livestock, but is usually added to animal feed such as livestock, poultry or aquaculture aquatic animals, or suspended in drinking water for administration. Administration is as long as necessary and usually lasts from one month to several years. Examples of animals to be administered include livestock such as cows, pigs, horses, and sheep, broilers, hens, quail, ducks, ducks,
Pheasants, poultry such as turkey, yellowtail, red sea bream, blowfish, tuna, flounder, striped horse mackerel, horse mackerel, salmon, carp, eel,
Examples include cultivated aquatic animals such as rainbow trout, sweetfish, shrimp (shrimp, button shrimp, lobster, black tiger, etc.) and crabs (red king crab, snow crab, blue crab, injured crab, etc.).
【0020】本発明の製剤を飼料に添加する場合、菌体
数が飼料1g当り約104 個以上、好ましくは約104
〜107 個、更に好ましくは約104 〜106 個となる
ように添加する。例えば賦形剤の入っていない製剤を使
用する場合、その添加量は0.1ppm以上、好ましく
は0.1〜1000ppm、更に好ましくは0.1〜1
00ppmで良い。又、賦形剤の入っている製剤を使用
する場合、0.001重量%以上添加でよく、好ましく
は0.01重量%〜10重量%、さらに好ましくは0.
1重量%〜0.5重量%でよい。When the preparation of the present invention is added to feed, the number of bacterial cells is about 10 4 or more per 1 g of feed, preferably about 10 4
It is added so as to be about 10 7 pieces, and more preferably about 10 4 to 10 6 pieces. For example, when a formulation containing no excipient is used, the amount added is 0.1 ppm or more, preferably 0.1 to 1000 ppm, more preferably 0.1 to 1 ppm.
00 ppm is sufficient. When a preparation containing an excipient is used, it may be added in an amount of 0.001% by weight or more, preferably 0.01% by weight to 10% by weight, more preferably 0.1% by weight.
It may be 1% by weight to 0.5% by weight.
【0021】本発明の製剤を飲水に添加する場合、菌体
数が飲水1ml当り約104 個以上、好ましくは約10
4 〜107 個、更に好ましくは約104 〜106 個程度
となるように添加する。例えば賦形剤の入っていない製
剤を使用する場合、その添加量は0.05ppm以上、
好ましくは0.05〜500ppm、更に好ましくは
0.05〜50ppm程度である。又、賦形剤の入って
いる製剤を使用する場合、その添加量は0.0005%
以上、好ましくは0.005%〜5%、さらに好ましく
は0.05%〜0.5%程度である。When the preparation of the present invention is added to drinking water, the number of cells is about 10 4 or more, preferably about 10 cells per 1 ml of drinking water.
It is added so as to be 4 to 10 7, more preferably about 10 4 to 10 6 . For example, when using a formulation containing no excipients, the amount added should be 0.05 ppm or more,
It is preferably 0.05 to 500 ppm, more preferably about 0.05 to 50 ppm. When using a formulation containing excipients, the amount added is 0.0005%.
The above is preferably 0.005% to 5%, more preferably about 0.05% to 0.5%.
【0022】次に、本発明の作用について実験例により
示す。 実験例1−1 ニジマスの発育試験 目的:ニジマスの育成用飼料にC.butyricum
MIYAIRIの死菌体を飼料に添加し、発育促進効
果をみるために以下の実験をおこなった。 実験方法 供試品:C.butyricum MIYAIRIの死
菌体を105 個/g添加した飼料を作成した。 実験手順:一匹当たり平均体重20gのニジマスを1区
525匹当て40日間飼育した。 試験結果を表1に示す。Next, the operation of the present invention will be shown by experimental examples. Experimental Example 1-1 Growth test of rainbow trout Purpose: C. butyricum
The dead cells of MIYAAIR were added to the feed, and the following experiment was conducted to examine the growth promoting effect. Experimental method Specimen: C. A feed was prepared by adding 10 5 dead cells of butyricum MIYAIRI / g. Experimental procedure: A rainbow trout having an average body weight of 20 g per animal was bred for 40 days for 525 animals per division. The test results are shown in Table 1.
【0023】[0023]
【表1】 表1 ニジマスの発育試験 日間増重量(g/尾/日) 飼料効率 死菌体投与区 0.23(110) 0.73(106) 生菌体投与区 0.21(100) 0.69(100) 対 照 区 0.21(100) 0.69(100) ────────────────────────────────── ( )は対照区を100とした指数で示した。 表1に示すように、C.butyricum MIYA
IRIの死菌体はニジマスに対する発育促進効果がある
ことが分かった。[Table 1] Table 1 Day-to-day growth test of rainbow trout Weight increase (g / tail / day) Feed efficiency Dead cell administration group 0.23 (110) 0.73 (106) Live cell administration group 0.21 (100) 0.69 (100) vs. 0.21 (100) 0.69 (100) ────────────────────────────── ───────────────────────────────────────────────────────────── rate each index As shown in Table 1, C. butyricum MIYA
It was found that dead cells of IRI have a growth promoting effect on rainbow trout.
【0024】実験例1−2 マウスの発育試験 目的:マウスの飼料にC.butyricum MIY
AIRIの死菌体を飼料に添加し、発育促進効果をみる
ために以下の実験をおこなった。 供試品:C.butyricum MIYAIRIの死
菌体を105 個/g、及び107 個/g、添加した飼料
を作成した。 実験手順:一匹当たり平均体重25gのSPFマウスを
1区10匹当て28日間飼育した。 試験結果を表2に示す。Experimental Example 1-2 Mouse Growth Test Purpose: C. butyricum MIY
The dead cells of AIRI were added to the feed, and the following experiment was conducted to examine the growth promoting effect. EUT: C. Butyricum MIYAIRI dead cells were added at 10 5 cells / g and 10 7 cells / g to prepare a feed. Experimental procedure: 10 SPF mice each having an average body weight of 25 g were applied to 1 group for 28 days. The test results are shown in Table 2.
【表2】 表2 マウスの発育試験 平均増体重量 対 照 区 2.95g(100) 死菌体105 個/g投与区 3.80 (129) 死菌体107 個/g投与区 4.50 (153) ───────────────────────────────── ( )は対照区を100とした指数で示した。 表2に示すように、C.butyricum MIYA
IRIの死菌体はマウスに対する発育促進効果があるこ
とが分かった。[Table 2] Table 2 Mouse growth test Average weight gain group 2.95 g (100) 10 5 dead cells / g administration group 3.80 (129) 10 7 dead cells / g administration group 4 .50 (153) ───────────────────────────────── () is an index with the control section as 100 It was As shown in Table 2, C. butyricum MIYA
It was found that killed cells of IRI have a growth promoting effect on mice.
【0025】実験例1−3 ブロイラーの発育試験 目的:ブロイラーの前期肥育用飼料にC.butyri
cum MIYAIRIの死菌体を飼料に添加し、発育
促進効果をみるために以下の実験をおこなった。 実験方法 供試品:C.butyricum MIYAIRIの死
菌体を106 個/g添加した飼料を作製した。 実験手順:平均体重40gのブロイラーを1区20羽当
て28日間飼育した。試験結果を表3に示す。Experimental Example 1-3 Growth test of broiler Purpose: C. butyri
The killed cells of cum MIYAIRI were added to the feed, and the following experiment was conducted to examine the growth promoting effect. Experimental method Specimen: C. A feed containing 10 6 dead cells of butyricum MIYAIRI / g was prepared. Experimental procedure: A broiler with an average body weight of 40 g was bred for 20 days per group for 28 days. The test results are shown in Table 3.
【表3】 表3 ブロイラーの発育試験 死菌体投与区 対照区 平均増体重 882g(104) 846g(100) 飼料要求率 1.57( 98) 1.61(100) ────────────────────────────────── ( )は対照区を100とした指数で示した。 表3に示すように、C.butyricum MIYA
IRIの死菌体はブロイラーに対する発育促進効果があ
ることが分かった。[Table 3] Table 3 Growth test of broiler control group killed cells average weight gain 882 g (104) 846 g (100) Feed conversion rate 1.57 (98) 1.61 (100) ─────── ─────────────────────────── () is shown as an index with the control plot as 100. As shown in Table 3, C. butyricum MIYA
It was found that killed cells of IRI have a growth promoting effect on broilers.
【0026】実験例1−4 養鰻飼料劣化試験(水中への散逸試験) 目的:養鰻飼料にC.butyricum MIYAI
RIの生菌、死菌体をそれぞれ添加し、劣化の有無をみ
るために以下の試験をおこなった。 試験方法 表4に示す飼料100gをビーカーにとり1.2倍量の
水を加え、もち状に練り上げた。次いで30℃10分間
放置後10gの団子状のかたまりを作り試料とした。な
おC.butyricum MIYAIRIは試料1g
中105 個相当量含有している。Experimental Example 1-4 Degradation test of eel feed (dissipation test into water) Purpose: C. butyricum MIYAI
The following tests were carried out in order to check the presence or absence of deterioration by adding live cells and dead cells of RI. Test method 100 g of the feed shown in Table 4 was placed in a beaker, 1.2 times the amount of water was added, and the mixture was kneaded into a sticky form. Then, the mixture was allowed to stand at 30 ° C. for 10 minutes, and 10 g of a lump-like lump was formed to obtain a sample. N. C. butyricum MIYAIRI is 1g sample
It contains 10 5 equivalents.
【0027】[0027]
【表4】 表4 試料組成 対照区 生菌区 死菌区 魚 粉 80% 80% 80% α化デンプン 20 19 19 生 菌 − 1 − 死 菌 − − 1 ─────────────────────────────── 各試料を300ml容三角フラスコに入れ、水を100
mlを加え、加温式振とう器で30分間振とうした。振
とう後5分間静置し、上澄液を採取して分光光度計(波
長660mμ)にて透過率を測定した。各試料5回測定
し、平均値を求めた。[Table 4] Table 4 Sample composition Control group Viable bacterial cell Dead bacterial cell Fish meal 80% 80% 80% Pregelatinized starch 20 19 19 Live bacteria-1-Death bacteria--1 ──────────── ───────────────────── Place each sample in a 300 ml Erlenmeyer flask and add 100 parts of water.
ml was added, and the mixture was shaken on a heating type shaker for 30 minutes. After shaking, the mixture was left standing for 5 minutes, the supernatant was collected, and the transmittance was measured with a spectrophotometer (wavelength 660 mμ). Each sample was measured 5 times and the average value was calculated.
【0028】試験結果を表5に示す。The test results are shown in Table 5.
【表5】 表5 養鰻飼料劣化試験(水中への散逸試験) 対照区 生菌区 死菌区 透過率% 56.5 31.8 57.6 (100) ( 56) (102) ─────────────────────────────── ( )は対照区を100とした指数で示した。 表5に示すように、死菌を用いることにより生菌による
水中での飼料の散逸が防止されることが分かった。[Table 5] Table 5 Deterioration test of eel feed (dissipation test in water) Control group Viable cell Dead cell group Permeability% 56.5 31.8 57.6 (100) (56) (102) ──── ─────────────────────────── () is shown as an index with the control plot as 100. As shown in Table 5, it was found that the use of dead bacteria prevented the dissipation of feed in water due to live bacteria.
【0029】実験例2−1 鶏ヒナの発育試験 試料: 本発明品:実施例2−3の製剤(菌数:8.7×1010
個/g) 対照品 :参考例の栄養体死菌末(菌数:5×1011
個/g) 実験手順:アーバーエーカー系のブロイラー雛(体
重44g)を1区当り20羽(雄10+雌10)×3反
復で60羽を用い、上記試料を菌体濃度(4.4〜4.
5)×105 個/gとなるように添加したブロイラー肥
育前期用飼料(抗生物質、抗菌剤無添加)を投与し、4
週間の発育試験を行なった。なお、上記試料を添加しな
い飼料を投与した区をブランクとした。 結果を下表に示す。Experimental Example 2-1 Developmental test of chicken chick Sample: Product of the present invention: Preparation of Example 2-3 (Number of bacteria: 8.7 × 10 10)
/ G) Control product: trophozoite dead end of reference example (bacteria number: 5 × 10 11
Number / g) Experimental procedure: Arbor-acre broiler chicks (body weight 44 g) were used in 20 groups (10 males + 10 females) x 3 replicates per group, and 60 cells were used in the above samples to obtain cell concentration (4.4-4). .
5) Administer the broiler feed for pre-fabrication (without addition of antibiotics and antibacterial agents) added so as to be 10 5 cells / g, and
A weekly growth test was conducted. In addition, the section to which the feed to which the above sample was not added was administered was used as a blank. The results are shown in the table below.
【0030】[0030]
【表6】 表6 ブロイラー発育試験 ────────────────────────────────── 試験区 増体量 飼料要求率 ────────────────────────────────── 無添加区 845g(100) 1.85(100) 対照品区 864g(102) 1.85(100) 本発明品区 884g(105) 1.81( 98) ────────────────────────────────── 上記実験例1−1〜1−4から明らかなようにC.bu
tyricum MIYAIRYAIRIの死菌体は家
畜、家禽、養殖水産動物の発育促進効果を有し、安全性
の高い畜肉及び養殖魚の生産に有効である。また、飼料
製造に当たり、生菌剤のような制限はなく、特に養魚飼
料に添加してもその劣化を促進することはないという生
菌剤にはないすぐれた効果を発揮する。又、上記実験例
2−1から明らかなように、C.butyricum
MIYAIRIの栄養体死菌末と糖及び/又は澱粉から
なる本発明の粉末製剤は、C.butyricum M
IYAIRIの栄養体死菌末のみよりも成長促進効果が
優れ、畜産物、水産物の生産性向上に有効である。[Table 6] Table 6 Broiler growth test ─────────────────────────────────── Test area Weight gain Dietary requirement Rate ────────────────────────────────── Additive-free group 845g (100) 1.85 (100) Control product Group 864g (102) 1.85 (100) Product of the present invention 884g (105) 1.81 (98) ────────────────────────── ───────── As is clear from the above Experimental Examples 1-1 to 1-4, C.I. bu
The dead bacterial cells of tyricum MIYAIRYAIRI have the effect of promoting the growth of livestock, poultry, and cultured aquatic animals, and are effective in the production of highly safe meat and cultured fish. In addition, in producing feed, there is no limitation like a probiotic agent, and even if it is added to a fish feed, it does not accelerate its deterioration, and it exhibits an excellent effect that a probiotic agent does not. Further, as is clear from Experimental Example 2-1, the C. butyricum
The powder formulation of the present invention comprising vegetatively killed MIYAIRI powder and sugar and / or starch is C.I. butyricum M
The growth promoting effect of IYAAIR is superior to that of vegetative dead bacteria powder alone, and it is effective in improving the productivity of livestock products and marine products.
【0031】[0031]
【実施例】以下に実施例により本発明を更に詳細に説明
する。 実施例1−1 散剤の製造 米糠油かす 96 g 大豆油 3 g ミヤイリ菌体末(死菌体1010/g) 1 g 上記の成分を混合し、散剤とする。The present invention will be described in more detail with reference to the following examples. Example 1-1 Production of powders Rice bran oil dregs 96 g Soybean oil 3 g Miyairi cell powder (dead cells 10 10 / g) 1 g The above components are mixed to give a powder.
【0032】実施例1−2 ペレットの製造 米糠油かす 70 g 小麦粉 28.9g ミヤイリ菌体末(死菌体1010/g) 1 g ポリアクリル酸ナトリウム 0.1g 上記の成分を混合し、ペレットマシンによりペレットす
る。Example 1-2 Production of Pellets Rice bran oil residue 70 g Wheat flour 28.9 g Miyairi cell powder (dead cells 10 10 / g) 1 g Sodium polyacrylate 0.1 g The above ingredients were mixed and pelletized. Pellet by machine.
【0033】実施例1−3 錠剤の製造 ミヤイリ菌体末(死菌体1010/g)1部に、乳糖80
部、コーンスターチ20部、低置換度ヒドロキシプロピ
ルセルロース20部及びポリビニルピロリドン2部を加
え十分混合後、造粒し、ステアリン酸マグネシウム0.
5部を加え混合後、錠剤する。Example 1-3 Production of tablets 1 part of Miyairi cell powder (dead cell body 10 10 / g) and lactose 80 parts
Part, corn starch 20 parts, low-substituted hydroxypropylcellulose 20 parts and polyvinylpyrrolidone 2 parts were added and mixed well, then granulated, and magnesium stearate 0.
Add 5 parts and mix, then tablet.
【0034】実施例1−4 細粒剤の製造 ミヤイリ菌体末(死菌体1010/g)1部に、マンニト
ール85部にカルボキシメチルセルロース15部及びヒ
ドロキシプロピルセルロース2.5部を加え、十分混合
し、造粒して細粒剤を得る。 実施例2−1Clostridium butyricum MIY
AIRI 588の保存株(FERM BP−278
9)を次の培地を用い、37°で培養し、16時間後、
培養液を遠心分離して栄養体期の菌体を得る。 ペプトン 1% 酵母エキス 1% ブドウ糖 1% 得られた菌体100g、コーンスターチ110g、乳糖
50g、炭酸カルシウム8gを水1Lに懸濁し、懸濁液
を噴霧乾燥機で熱風の入口温度140〜160℃、出口
温度70°〜95°で、瞬間的に乾燥粉末とし、本発明
の製剤を得る。Example 1-4 Production of fine granules To 1 part of Myria spp. Powder (dead cells 10 10 / g), mannitol 85 parts, carboxymethyl cellulose 15 parts and hydroxypropyl cellulose 2.5 parts were added, and the mixture was thoroughly mixed. Mix and granulate to obtain a fine granule. Example 2-1 Clostridium butyricum MIY
Stock strain of AIRI 588 (FERM BP-278
9) was cultivated at 37 ° using the following medium, and after 16 hours,
The culture solution is centrifuged to obtain vegetative cells. Peptone 1% Yeast extract 1% Glucose 1% 100 g of the obtained bacterial cells, 110 g of corn starch, 50 g of lactose, 8 g of calcium carbonate were suspended in 1 L of water, and the suspension was spray-dried at a hot air inlet temperature of 140 to 160 ° C. The powder of the present invention is obtained by instantaneously making a dry powder at an outlet temperature of 70 ° to 95 °.
【0035】実施例2−2 ミヤイリ菌の栄養体死菌末300g、乳糖100g、と
うもろこし澱粉600gをv型混合基で混合して粉末状
の本発明の製剤を得る。 実施例2−3 ミヤイリ菌の栄養体死菌末5.15kg、乳糖2kg、
とうもろこし澱粉13kg、炭酸カルシウム1kgに水
を加えて乳化し、更に水を加えて100Lとし、スプレ
ードライヤーにて噴霧乾燥し粉末状の本発明の製剤を得
た。 実施例2−4 ミヤイリ菌の栄養体死菌末2.5kg、乳糖0.5gk
g、とうもろこしの澱粉7kg、ビール酵母90kgを
ドラム型混合機で混合して粉末状の本発明の製剤を得
る。Example 2-2 300 g of vegetatively killed Mycobacterium leprae bacillus powder, 100 g of lactose, and 600 g of corn starch were mixed with a v-type mixing group to obtain a powdery preparation of the present invention. Example 2-3 5.15 kg of vegetatively killed vegetative cells of Mycobacterium leprae, 2 kg of lactose,
Water was added to 13 kg of corn starch and 1 kg of calcium carbonate to emulsify, and water was further added to make 100 L, and the mixture was spray-dried with a spray dryer to obtain a powdery preparation of the present invention. Example 2-4 2.5 kg of killed vegetative body of Myria spp., 0.5 gk of lactose
g, 7 kg of corn starch, and 90 kg of brewer's yeast are mixed in a drum mixer to obtain a powdery preparation of the present invention.
【0036】実施例2−5 実施例1の噴霧乾燥品5g、ビール酵母85g、脱脂米
ぬか10gを均一に混合して、本発明の製剤を得る。 参考例Clostridium butyricum MIY
AIRI 588の保存株(FERM BP−278
9)を次の培地を用い、37°で培養し、16時間後、
培養液を大気中で遠心分離して栄養体期の菌体を得た。 ペプトン 1% 酵母エキス 1% ブドウ糖 1% 得られた菌体100gを水350mlに懸濁し、この懸
濁液を、予め−30℃で予備凍結を行った後、常法によ
り凍結乾燥を行って、乾燥粉末とし、栄養体死菌体末を
得た。Example 2-5 5 g of the spray-dried product of Example 1, 85 g of brewer's yeast and 10 g of defatted rice bran were uniformly mixed to obtain the preparation of the present invention. Reference example Clostridium butyricum MIY
Stock strain of AIRI 588 (FERM BP-278
9) was cultivated at 37 ° using the following medium, and after 16 hours,
The culture was centrifuged in the atmosphere to obtain vegetative cells. Peptone 1% Yeast extract 1% Glucose 1% 100 g of the obtained bacterial cells was suspended in 350 ml of water, and this suspension was preliminarily frozen at -30 ° C and then freeze-dried by a conventional method. Dry powder was obtained to obtain vegetative dead bacterial cell powder.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 水落 一雄 埼玉県鴻巣市赤見台3−26−17 (72)発明者 浅野 猛 群馬県藤岡市立石871−5 (72)発明者 村山 隆一 群馬県高崎市岩鼻町239 日化AP F− 34 (72)発明者 北城 俊男 長野県長野市若穂川田3274−3 (72)発明者 田中 守 長野県埴科郡戸倉町大字戸倉2440 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kazuo Mizuochi 3-26-17 Akamidai, Konosu City, Saitama Prefecture (72) Inventor Takeshi Asano 871-5 Tateishi, Fujioka City, Gunma Prefecture (72) Inventor Ryuichi Murayama Takasaki, Gunma Prefecture 239 Iwahana-cho, Ichihana Nikka AP F-34 (72) Inventor Toshio Kitagi 3274-3 Wakaho Kawada, Nagano City, Nagano Prefecture (72) Inventor Mamoru Tanaka 2440 Tokura, Tokura Town, Hanashina-gun, Nagano Prefecture
Claims (26)
菌体を動物に経口投与することを特徴とする動物の成長
促進方法。1. A method for promoting growth of an animal, which comprises orally administering to the animal killed cells of a microorganism belonging to the genus Clostridium.
ロストリジウム・ブチリカム種に属する微生物である請
求項1の方法。2. The method according to claim 1, wherein the microorganism belonging to the genus Clostridium is a microorganism belonging to the species Clostridium butyricum.
る微生物がクロストリジウム・ブチリカム・ミヤイリで
ある請求項2の方法。3. The method according to claim 2, wherein the microorganism belonging to the species Clostridium butyricum is Clostridium butyricum miyairi.
方法。4. The method according to claim 1, wherein the dead cells are vegetative dead cells.
請求項1の方法。5. The method according to claim 1, wherein the dead cells are added to the feed and administered to the animal.
個/g以上である請求項5の方法。6. The amount of dead cells in the feed is 10 4 as the number of cells.
6. The method according to claim 5, wherein the number is not less than 1 piece / g.
求項1の方法。7. The method of claim 1, wherein the animal is livestock, poultry or aquatic animals.
養体死菌体と糖及び/又は澱粉を必須成分とするクロス
トリジウム属菌死菌体粉末製剤。8. A powdered preparation of dead Clostridium spp. Cells containing, as essential components, vegetative dead cells of a microorganism belonging to the genus Clostridium and sugar and / or starch.
ロストリジウム・ブチリカム種に属するものである請求
項8の製剤。9. The preparation according to claim 8, wherein the microorganism belonging to the genus Clostridium belongs to the species Clostridium butyricum.
する微生物がクロストリジウム・ブチリカム・ミヤイリ
である請求項9の製剤。10. The preparation according to claim 9, wherein the microorganism belonging to the species Clostridium butyricum is Clostridium butyricum Miyairi.
6 個以上である請求項8の製剤。11. The amount of killed vegetative cells is 10 per 1 g of the preparation.
The formulation according to claim 8, which is 6 or more.
6 個〜1011個である請求項8の製剤。12. The amount of dead vegetative cells is 10 per 1 g of the preparation.
The formulation of claim 8, which is 6 to 10 11 .
糖又は乳糖であり、澱粉が小麦澱粉、馬鈴薯澱粉、トウ
モロコシ澱粉である請求項8の製剤。13. The preparation according to claim 8, wherein the sugar is glucose, fructose, sucrose, oligosaccharide or lactose, and the starch is wheat starch, potato starch or corn starch.
が、クロストリジウム属に属する微生物の栄養死菌体1
重量部に対し、糖が0.04〜700重量部であり、澱
粉が0.2〜700重量部である請求項8の製剤。14. A vegetatively killed microbial cell 1 of a microorganism belonging to the genus Clostridium, wherein the sugar and / or starch content in the preparation is 1.
The formulation according to claim 8, wherein the sugar is 0.04 to 700 parts by weight and the starch is 0.2 to 700 parts by weight based on parts by weight.
・ミヤイリの栄養体死菌体0.00005〜80w/w
%; (2)糖類 0.001〜50w/w%; (3)澱粉 0.015〜90w/w%; (4)水難溶性無機物 0.0005〜33w/w%;
及び (5)賦形剤 1〜99.9w/w% を含有し、栄養体死菌体の菌体数が製剤1g当り、10
6 個以上である請求項8の製剤。15. (1) Clostridium butyricum miyairi vegetatively dead microbial cells 0.00005 to 80 w / w
%; (2) Sugar 0.001 to 50 w / w%; (3) Starch 0.015 to 90 w / w%; (4) Poorly water-soluble inorganic substance 0.0005 to 33 w / w%;
And (5) Excipients 1 to 99.9 w / w% are contained, and the number of vegetative cells is 10 per 1 g of the preparation.
The formulation according to claim 8, which is 6 or more.
炭酸カルシウムである請求項15の製剤。16. The preparation according to claim 15, wherein the saccharide is lactose and the poorly water-soluble inorganic substance is calcium carbonate.
栄養体死菌体と糖及び/又は澱粉を水に懸濁後、噴霧乾
燥することを特徴とするクロストリジウム属菌死菌体粉
末製剤の製法。17. A method for producing a powdered preparation of killed Clostridium bacteria, which comprises suspending vegetatively killed bacteria cells of a microorganism belonging to the genus Clostridium and sugar and / or starch in water and then spray-drying.
死菌体を有効成分とする動物の成長促進剤。18. An animal growth promoter comprising, as an active ingredient, dead cells of a microorganism belonging to the genus Clostridium.
18の成長促進剤。19. The growth promoting agent according to claim 18, wherein the dead cells are vegetative dead cells.
クロストリジウム・ブチリカム種である請求項18の成
長促進剤。20. The growth promoting agent according to claim 18, wherein the microorganism belonging to the genus Clostridium is Clostridium butyricum species.
クロストリジウムブチリカム・ミヤイリである請求項1
8の成長促進剤。21. The microorganism belonging to the genus Clostridium is Clostridium butyricum miyairi.
8 growth promoters.
死菌体粉末0.00005〜80%、残部が担体である
クロストリジウム属死菌体組成物。22. A composition of killed bacterium belonging to the genus Clostridium, wherein 0.00005 to 80% of killed cell powder of a microorganism belonging to the genus Clostridium, and the rest being a carrier.
求項22の組成物。23. The composition according to claim 22, wherein the dead cell powder is a vegetative dead cell.
死菌体粉末を0.01〜50%含む請求項21の組成
物。24. The composition according to claim 21, which contains 0.01 to 50% of dead cell powder of a microorganism belonging to the genus Clostridium.
クロストリジウム・ブチリカム種である請求項22の組
成物。25. The composition of claim 22, wherein the microorganism belonging to the genus Clostridium is Clostridium butyricum sp.
クロストリジウム・ブチリカム・ミヤイリである請求項
22の組成物。26. The composition according to claim 22, wherein the microorganism belonging to the genus Clostridium is Clostridium butyricum miyairi.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4330508A JP2799273B2 (en) | 1991-12-11 | 1992-12-10 | Method for promoting animal growth and powdered preparation of killed cells of Clostridium sp. |
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP32770791 | 1991-12-11 | ||
JP4-285834 | 1992-10-23 | ||
JP28583492 | 1992-10-23 | ||
JP3-327707 | 1992-10-23 | ||
JP4330508A JP2799273B2 (en) | 1991-12-11 | 1992-12-10 | Method for promoting animal growth and powdered preparation of killed cells of Clostridium sp. |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10107716A Division JP2987359B2 (en) | 1991-12-11 | 1998-04-17 | Method for promoting animal growth and powdered preparation of killed cells of Clostridium sp. |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH06181696A true JPH06181696A (en) | 1994-07-05 |
JP2799273B2 JP2799273B2 (en) | 1998-09-17 |
Family
ID=27337195
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP4330508A Expired - Lifetime JP2799273B2 (en) | 1991-12-11 | 1992-12-10 | Method for promoting animal growth and powdered preparation of killed cells of Clostridium sp. |
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JP (1) | JP2799273B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2018516567A (en) * | 2015-05-21 | 2018-06-28 | ランザテク・ニュージーランド・リミテッド | Gas fermentation for the production of protein or feed |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63146825A (en) * | 1986-12-11 | 1988-06-18 | Tadashi Arai | Drug for controlling intestinal function |
JPH01153645A (en) * | 1987-12-10 | 1989-06-15 | Nippo Kagaku Kk | Preventive and treating agent for toxinogenic escherichia coli infection |
-
1992
- 1992-12-10 JP JP4330508A patent/JP2799273B2/en not_active Expired - Lifetime
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63146825A (en) * | 1986-12-11 | 1988-06-18 | Tadashi Arai | Drug for controlling intestinal function |
JPH01153645A (en) * | 1987-12-10 | 1989-06-15 | Nippo Kagaku Kk | Preventive and treating agent for toxinogenic escherichia coli infection |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2018516567A (en) * | 2015-05-21 | 2018-06-28 | ランザテク・ニュージーランド・リミテッド | Gas fermentation for the production of protein or feed |
Also Published As
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JP2799273B2 (en) | 1998-09-17 |
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