JPH06138086A - Method and device for preparing gel - Google Patents

Abstract

PURPOSE:To reduce the working time and surely seal the edge face of a plate by sealing the edge face by using a frame body having a depth which is the same as the thickness of a double-glass plate without using any adhesive tape. CONSTITUTION:Two U-shaped flexible frame bodies which have strong restoring forces and are expanded with a thin rubber sheet 2 in between are placed on and fixed to a table 1. After a lower glass plate 5 cut in accordance with the inner edge of the upper frame body is put in the upper frame body from the top and spacers 7 corresponding to the desired thickness of gel are put on both sides of the plate 5, an upper glass plate 4 is put on and pressed against the plate 5 so that the thin rubber sheet 2 can closely adhere to the plate 5. The upper surface of the plate 4 is flush with each upper surface of the upper frame body and the gap 9 between the edge end face of the pate 4 and the inner edge of the upper frame body is sealed with an adhesive sheet. Then, the table 1 is tilted so that the opened side of the frame bodies can come to the top side while the entire surface of the plate 5 is evenly heated with a heater 6 and a gel solution is injected into the whole space 11 corresponding to the thickness of the spacers 7 between the plates 4 and 5 through narrow holes formed through the sides of the lower frame body. Thus, a gel layer corresponding to the spacers 7 is formed.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a flat plate electrophoresis apparatus and a flat plate gel making apparatus. Here, the flat gel means that two equal-sized transparent flat glass plates are stacked with a slight gap,
The gel solution was thinly injected into the entire surface of the gap and solidified. During the electrophoretic analysis, the plate-like gel is loaded in an electrophoretic device, and the electrophoretic pattern is to be observed through the transparent glass plate.

[0002]

2. Description of the Related Art Conventionally, this gel has been prepared manually. That is, two spacers for setting the above-mentioned gap are used.
The two glass plates are sandwiched between the rectangular glass plates and along the periphery of the three glass plates, and the two glass plates are crimped by a clip so as not to shift the position of the spacer and the spacer. Next, with one continuous adhesive tape, while removing the clip so as to wrap the edge surfaces of the two stacked glass plates in a U-shape, wind the glass plate around the above three areas,
Rub the tape thoroughly from above so that the tape adheres to the glass plate. The glass plate is tilted so that the edge surface of the remaining one side faces upward, and the gel solution is poured from the edge. After allowing the gel solution to spontaneously solidify, the adhesive tape is peeled off, and the glass plate sandwiching the gel is loaded into an electrophoresis machine for electrophoretic analysis.

[0003]

In this case, since the periphery of the glass plate stack 3 is sealed with an adhesive tape, in principle, this does not leak when the gel solution is injected. -It was often the case that the sealing operation was manual and that the tape could not be completely sealed at the right-angled corners of the glass plate due to poor adhesion of the tape. However, the above adhesive tape is peeled off when the glass plate is loaded into the electrophoretic apparatus, but cannot be reused. The above manual work is time consuming, unreliable and uneconomical. Therefore, the present invention provides a gel making device corresponding to the above jig for making gel, and not only shortens the working time, but also reduces consumables such as an adhesive tape as much as possible to ensure a reliable seal. The goal is to achieve

[0004]

In order to solve the above-mentioned drawbacks of the conventional type, in the present invention, the edge surface of the double glass plate is sealed without using the above-mentioned adhesive tape. Prepare the jig to be used. This jig is basically a frame and is composed of a frame in which the thickness of the double glass plate and the depth of the frame are substantially the same. In order to cover the frame body, a thin plate such as silicon rubber having an extremely high elasticity is used to form a film in the middle portion of the thickness of the frame body. When the above-mentioned two-layered glass plates are fitted from one surface of this frame, the thin plate extends, and the glass plate of the double glass plate, which is one side, is one rubber thin plate and is used as a flat surface and an edge surface. Is completely wrapped. In this case, the rubber thin plate is tightly attached to the edge surface of the bent corner of the glass plate at a right angle. On the other hand, the space gap between the edge surface of the glass plate which is not wrapped with the rubber thin plate and the inner surface of the frame in which the glass plate is fitted is adhered between the peripheral surface of the glass plate and the upper surface of the frame. See
Seal by sticking toe. Since the glass plate is relatively heavy, it is preferable that the frame is placed on a table, the box is used as a bottom plate, and the glass plate is dropped into the frame from above. When injecting the gel solution, the table on which the above glass plate is placed is tilted, and the gel solution is injected into the gap between the two glass plates from the lowered edge thereof. For this reason, pores are opened in the frame portion of this edge, and the gel solution is injected under pressure from here. Further, a heater is provided on the entire surface of the table, which promotes the solidification of the gel solution and keeps the polymerization degree constant without being affected by the ambient temperature.

[0005]

EXAMPLE Two U-shaped plates 3 spread with a thin rubber plate 2 which is flexible and has a large restoring force are placed and fixed on a table 1 on a flat plate. A heater 6 is provided on the lower surface of the base 1 so that the entire surface area surrounded by the U-shaped plate 3 can be heated. The inner edge of the U-shaped plate almost matches the outer shape of the glass plate,
The glass plates 4 and 5 are designed to fit snugly. The lower glass plate 5 is dropped from above the thin rubber plate 2 so as to be aligned with the inner edge of the U-shaped plate 3, and the spacers corresponding to the desired thickness of the gel are formed on the left and right sides of the lower glass plate 5. After placing -7, lower glass plate 5
The upper glass plate 4 is installed so as to be overlapped with. The upper glass plate 4 is pushed toward the surface of the rubber plate 2 to bring the rubber plate 2 and the lower glass plate 5 into close contact with each other. At this time, the upper surfaces of the upper glass plate 4 and the U-shaped plate are flush with each other, and the upper surfaces of the three sides of the U-shaped plate and the upper surface of the upper glass plate 4 are bonded to each other. Sheet 8
The gap between the inner edge of the U-shaped plate and the edge surface of the glass plate is closed. In this state, the gap space 9 between the upper and lower glass plates is sealed by the rubber plate 2 and the adhesive sheet 8. The heater 6 heats the entire surface of the lower glass plate at a constant temperature, and the table is inclined so that the open side of the U-shaped plate faces upward. In this case, a thin hole 10 is provided in the central part of the plate above the two U-shaped plates on the lowered side, and the gel solution is injected from this hole 10 by a pump and is inserted between the upper and lower glass plates. The entire layer 11 corresponding to the thickness of the spacer is filled. In addition, among the above, the adhesive sheet
As a method other than pasting the grate 8, a rubber plate made of the same material is pressed or removed on the same part (a part that straddles the upper surface of the three sides of the U-shaped plate and the upper surface of the upper glass plate 4). It is also possible to provide a mechanism for closing and opening the gap space 9.

[0006]

EFFECTS OF THE INVENTION According to the present invention, it is not necessary to seal the edge surface of the glass plate with an adhesive tape, which is economical and requires no skill to improve workability and reliability. did. Since the gel solution is continuously injected from the lower part of the glass plate, it is possible to prevent bubbles from being mixed in, and it is also possible to provide a continuous gradient of the gel solution composition concentration in the vertical direction between the glass plates. . By controlling the temperature of the gel solution filled between the glass plates with a heater installed on a table, it was possible to obtain the same degree of polymerization every time without being influenced by the surrounding environment. In particular, acrylamide as a gel solution is accompanied by heat generation when it is polymerized to form a polyacrylamide gel, but the calorific value varies depending on the location, and the gel may have a non-uniform degree of polymerization. -By controlling the temperature of the entire surface, it is possible to obtain a homogeneous gel with a uniform degree of polymerization.

[Brief description of drawings]

FIG. 1 is a cross-sectional view of a gel making device according to the present invention.

2A, 2B, and 2C are views for explaining the procedure for preparing a gel according to the present invention. In particular, FIG. 2C is a sectional view taken along the line AA ′ of FIG. 2B.

[Explanation of symbols]

 1: Stand 2: Rubber thin plate 3: U-shaped plate 4: Upper glass plate 5: Lower glass plate 6: Heater 7: Spacer 8: Adhesive sheet 9: Upper glass plate Gap between edge surface and frame 10: Gel solution injection hole

Claims (2)

[Claims] 1. When preparing an electrophoresis gel between two glass plates of equal thickness and size, the thickness of the glass plates and the thickness of the glass plates are set so that these two glass plates can be exactly dropped. A U-shaped frame body having the same depth is prepared, a thin rubber film is spread in the middle of the depth of the frame body, and the frame body is placed on a horizontal stand, and The glass plate is fitted into the frame body, and the gap between the upper surface glass plate and the frame body is sealed with an adhesive tape so that the open side of the U-shaped frame body faces upward. The frame body is inclined, and in this case, the gel solution is pressure-injected into the glass plate from the outside through pores provided on the side of the frame body which is the lower side, and at the same time, the gel solution is heated to a constant temperature and solidified How to make gel. 2. A glass plate having the same thickness and the same size, which is stacked with a slight gap, has the same depth as the thickness of the glass plate so that the two glass plates can be exactly dropped. It is provided on the U-shaped frame body in which the rubber thin film is spread on the part, the stand on which the U-shaped frame body is mounted and fixed, and the frame body on the side facing the open side of the U-shaped frame body. A gel making device comprising: a gel solution injection pore communicating with the glass plate gap; and a heater provided on the fixing table for heating the glass plate at a constant temperature.