JPH06125771A - Lactic acid proliferating agent and its production - Google Patents

Abstract

PURPOSE:To obtain an inexpensive and excellent lactic acid proliferating agent having proliferation promoting effect, free from characteristic taste accompanied by unpleasant odor, excellent in safety to human and capable of promoting proliferation of lactic acid bacteria such as general lactic acid bacterium and Bifidobacterium. CONSTITUTION:This lactic acid bacterium-proliferating agent contains an extract extracted from leaves of a plant of the genus Coffee as a main ingredient.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a lactic acid bacterium proliferating agent that promotes the growth of general lactic acid bacteria and lactic acid bacteria such as bifidobacteria which are useful in the intestines of humans or used for food production and the like, and a method for producing the same. Is.

[0002]

2. Description of the Related Art General lactic acid bacteria such as lactobacillus and lactococcus and lactic acid bacteria such as bifidobacteria are used as a means for producing foods such as yogurt and pharmaceuticals such as intestinal regulators or components thereof, and their usefulness is a health boom in recent years. With the rise of The lactic acid bacteria belong to the heterotrophic bacteria and exhibit complex nutritional requirements. That is, examples of auxotrophic nutrients in the case of general lactic acid bacteria include nitrogen compounds, carbon compounds, inorganic salts, vitamins, amino acids, peptides and the like, and in the case of Bifidobacterium, monosaccharides and oligosaccharides in addition to the above auxotrophic nutrients. (Oligosaccharides) can be mentioned. Therefore, in order to promote the growth of lactic acid bacteria such as the general lactic acid bacterium and bifidobacteria, in a medium based on the auxotrophic nutrient, the composition of the auxotrophic nutrient is examined, and a medium effective for the growth of the lactic acid bacterium is prepared. Is being done. In addition, lactic acid bacteria growth agents such as yeast extract, peptone, and cystine have been added to a medium based on the above required nutrients.

[0003]

However, the above-mentioned yeast extract, peptone, cystine and other lactic acid bacteria proliferating agents are
It is not approved as an additive to general foods.
In addition, the above-mentioned required nutrients and lactic acid bacteria proliferating agents and media containing these have a unique odor and taste, and some have an unpleasant sensation, and thus have the problem of poor versatility in use in foods. Have. Furthermore, the above-mentioned lactic acid bacteria proliferating agent is expensive and has a cost problem.

The present invention has been made in view of such circumstances, has an excellent effect of promoting proliferation at low cost, does not have a unique flavor accompanied by discomfort, and is directly applied not only to the field of pharmaceuticals but also to foods. It is an object of the present invention to provide a lactic acid bacterium growth agent that can be added and is excellent in human safety and that promotes the growth of general lactic acid bacteria and lactic acid bacteria such as bifidobacteria.

[0005]

In order to achieve the above-mentioned object, the present invention has as its first gist a lactic acid bacterium-proliferating agent whose main component is an extract extracted from leaves of a coffee plant, and leaves of a coffee plant. The second gist is a method for producing a lactic acid bacterium proliferating agent for extracting water with water, and the third gist is a method for producing a lactic acid bacterium proliferating agent for concentrating an extract obtained by water-extracting leaves of a coffee tree plant into a paste. Extraction obtained by water-extracting leaves of a coffee tree plant is defined as the fourth gist of the method for producing a lactic acid bacterium-growing agent by adding an emulsifier and oil and fat to an extract obtained by water-extracting leaves of a coffee tree plant. A fifth method is a method for producing a lactic acid bacterium-growing agent for drying a liquid, and a sixth method is a method for producing a lactic acid bacterium-growing agent, which comprises removing an oil-soluble component of an extract obtained by water-extracting leaves of a coffee tree plant and concentrating and solidifying the extract. The summary of

[0006]

[Effects] That is, the present inventors have found that lactic acid bacteria such as general lactic acid bacteria and bifidobacteria, which have an excellent growth promoting effect at low cost, have no unique flavor accompanied by discomfort, and have excellent safety for humans, etc. A series of studies were conducted in order to obtain a lactic acid bacteria growth agent that promotes growth. In the process of that research,
The idea was to use the leaves of the genus Coffea, which is widely cultivated in tropical and subtropical regions, as a raw material for coffee. Since the coffee tree collects beans as a coffee raw material, waste leaves are cut down to increase the yield of beans, and the cut down waste leaves are discarded. Therefore, the felled waste leaves have an advantage that the raw material cost is almost unnecessary. Further, it has been found that there is no problem in safety even when ingested by humans as a food or a medicine, and it does not have a unique flavor accompanied by discomfort. Therefore, as a result of further studies for utilizing the leaves of the coffee plant as a lactic acid bacterium proliferating agent for lactic acid bacteria, the lactic acid bacterium proliferating agent mainly comprising the extract of the leaves of the coffee genus plant is a general lactic acid bacterium and bifidobacteria. In the growth of lactic acid bacteria such as the above, the present invention was found to exhibit a remarkable growth promoting effect as compared with the conventional lactic acid bacteria growth agents.

Next, the present invention will be described in detail.

The lactic acid bacteria-proliferating agent of the present invention can be obtained, for example, by using an extract obtained by water-extracting leaves of a coffee plant (hereinafter referred to as "coffee leaves") as a lactic acid bacteria-proliferating agent. .

The coffee leaf is not particularly limited as long as it is a leaf of a plant of the genus Coffea, but generally, it is a cultivated species that is easily available, such as Arabica and Robusta, alone or 2 Used in combination with more than one type. As the above-mentioned coffee leaves, new shoots or young leaves that are cut off as waste leaves during coffee cultivation are suitable in terms of cost.

The above-mentioned coffee leaves may be used as they are, but it is preferred that they are washed with water and air-dried at room temperature for 1 to 2 weeks. That is, by removing the water to some extent, the coffee leaves will not rot and the storability will be improved.

The coffee leaf may be fermented. That is, it is also possible to use the one which is subjected to withering with direct sunlight for 5 to 8 hours after the collection and then fermented while occasionally stirring, and then dried with hot air at 50 to 80 ° C. for about 1 hour.

As a method of obtaining the extract from the coffee leaves, it is preferable to carry out water extraction, and particularly hot water extraction is most suitable. That is, the coffee leaf is immersed in water and boiled at 90 to 100 ° C. for a predetermined time to extract the components in the coffee leaf into water. Then, by filtering the obtained extract to remove coffee leaves,
An extract can be obtained. It is preferable to use distilled water as the water used for the hot water extraction. The concentration of the extract is preferably set within a range of 15 to 23% by weight (hereinafter, abbreviated as "%") as a solid content concentration calculated back from the solid content when the obtained extract is dried. Is.

The extract obtained from the above coffee leaves is
Besides being used in liquid form, the extract may be concentrated and used as a paste-like concentrate. It is preferable that the paste-like concentrate has a fluidity of about 50 to 3000 cps at room temperature.

It is also possible to use the extract obtained from the coffee leaves as a dried product having a water content of 1% or less by freeze-drying, spray-drying or the like.

Furthermore, the extract obtained from the leaves of the coffee, extracts the oil soluble components to with a solvent such as chloroform, was removed, then extracted water component added ethyl acetate, anhydrous Na ₂ Add SO ₄ etc. to adsorb water,
After filtering to remove anhydrous Na ₂ SO ₄ etc. adsorbing water, the mixture was distilled and concentrated to remove the above ethyl acetate etc. to obtain a purified solid product of coffee leaf extract. It is also possible to use.

Then, an emulsifier such as glycerin fatty acid ester and an oil and fat are added to the extract obtained from the coffee leaf to emulsify it, and this emulsion of the extract can also be used as a lactic acid bacterium growth agent. The addition amount of the emulsifier and the fat and oil is preferably set within the range of 0.002 to 0.05% of the total extract. It is also possible to use a concentrated paste-like concentrate or a dried product obtained by subjecting the extract emulsion to a lactic acid bacterium growth agent.

Further, it is also possible to use the above-mentioned non-fermented or fermented coffee leaves by finely cutting them into powder without performing operations such as extraction of the coffee leaves. That is, since the action of growth factors of lactic acid bacteria such as general lactic acid bacteria and bifidobacteria contained in coffee leaves is strong, even if the coffee leaf powder is used without performing an extraction operation such as the water extraction. This is because it exerts a growth promoting effect on lactic acid bacteria.

As described above, the lactic acid bacterium growth agent of the present invention can be used in various forms. That is, the growth factors that promote the growth of general lactic acid bacteria and lactic acid bacteria such as bifidobacteria contained in coffee leaves are chemically and physically stable, and even if they are subjected to treatments such as heating, concentration, drying and emulsification. This is because the effect is not lost.

The coffee leaf extract, pasty concentrate, dried product, purified solid matter, extract emulsion, etc. may be used alone or together with live bacteria of general lactic acid bacteria and bifidobacteria, or in addition to the above. It can be processed into tablets, powders, liquids and the like together with the medicinal components of (1) above to provide an intestinal regulating agent having a proliferative effect on useful intestinal bacteria such as general lactic acid bacteria and bifidobacteria. In addition, a functional food having an intestinal regulating function can be prepared by adding the above-mentioned coffee leaf extract or the like to an ordinary food. further,
In the production of foods and pharmaceuticals using lactic acid bacteria such as yogurt as a processing means, the above-mentioned coffee leaf extract or the like can be added and used as a production auxiliary agent.

[0020]

INDUSTRIAL APPLICABILITY As described above, since the lactic acid bacteria proliferating agent of the present invention can contain as a main component an extract extracted from leaves of a coffee genus plant that is to be disposed of in large quantities in a coffee farm, it is stable. This enables low cost manufacturing. In addition, the leaves of the above-mentioned coffee genus plant have no peculiar flavor accompanied by discomfort, and there is no problem in safety for humans. Therefore, it can be used for products directly ingested by humans, such as foods and pharmaceuticals. Furthermore, since the growth factors of general lactic acid bacteria and lactic acid bacteria such as Bifidobacterium in the lactic acid bacterium growth agent of the present invention are physically and chemically stable, it is sufficient to use the lactic acid bacterium growth agent of the present invention as a raw material together with other components. It is possible to easily produce a medium, food, drug, etc. having the above-mentioned lactic acid bacterium growth effect. further,
Since the lactic acid bacterium proliferating agent of the present invention has an excellent effect of promoting the growth of lactic acid bacteria such as general lactic acid bacteria and bifidobacteria, if this is used as a processing aid, the period required for the production of foods and pharmaceuticals using the lactic acid bacterium as a processing means Can be shortened, and manufacturing efficiency can be improved.
Since the lactic acid bacteria proliferating agent of the present invention can be used in various forms such as a liquid substance, a paste-like concentrate, a dried substance, an extract emulsion, etc. It becomes possible to apply it widely to etc. Moreover, since the method for producing the lactic acid bacterium-growing agent of the present invention is simple, it is possible to produce the lactic acid bacterium-growing agent by a conventionally known simple apparatus and method without using a special apparatus and method.

Next, examples will be described.

[0022]

Example 1 After rinsing with water, leaves of the coffee genus Arabica coffee species collected at a coffee growing farm in Kona, Hawaii, USA, were spread on a cloth, air-dried at room temperature with occasional stirring for 10 days, Non-fermented coffee leaves were obtained. The water content of the obtained non-fermented coffee leaves was 9.3%. Next, the above non-fermented coffee leaves 1
Distilled water (2850 parts) was added to 50 parts by weight (hereinafter abbreviated as "part") to make a total of 3000 parts, and hot water extraction was performed at 90 to 100 ° C for 2 hours, followed by filtering to remove coffee leaves to obtain an extract. It was The solid concentration of the above extract was 1.2%.

[0023]

Example 2 The extract obtained in Example 1 was concentrated using an evaporator at an upper limit temperature of 45 ° C. until the volume became 1/10 to obtain a paste-like concentrate. The solid content concentration of the paste-like concentrate was 13%.

[0024]

Example 3 The paste-like concentrate obtained in Example 2 was dried using a freeze dryer to obtain a dried product. The water content of the dried product was 0.4%.

[0025]

Example 4 To the extract obtained in Example 1, glycerin fatty acid ester as an emulsifier and edible oil as an oil and fat were added at a ratio of 0.05% of the whole extract, and mixed by stirring to obtain an emulsion of the extract. Got

[0026]

Example 5 The emulsion of the extract obtained in Example 4 was concentrated using an evaporator at an upper limit temperature of 45 ° C. until the volume became 1/10 to obtain a paste-like concentrate. The solid content of the pasty concentrate was 12%.

[0027]

[Example 6] The extract emulsion obtained in Example 4 was dried using a freeze dryer to obtain a dried product. The water content of the dried product was 0.4%.

[0028]

[Example 7] Leaves of the same coffee genus Arabica coffee species as in Example 1 were withered by irradiation with sunlight for 6 hours, and 60
Fermented coffee leaves were obtained by hot-air drying at ℃ for 1 hour. The water content of the fermented coffee leaves was 10%. Next, the same hot water extraction as in Example 1 was performed to obtain an extract having a solid content concentration of 1.3%.

[0029]

Example 8 The extract obtained in Example 7 was concentrated using an evaporator at an upper limit temperature of 45 ° C. until the volume became 1/10 to obtain a paste-like concentrate. The solid content of the paste-like concentrate was 14%.

[0030]

Example 9 The paste-like concentrate obtained in Example 8 was dried using a freeze dryer to obtain a dried product. The water content of the dried product was 0.4%.

[0031]

Example 10 To the extract obtained in Example 7, glycerin fatty acid ester as an emulsifier and edible oil as an oil and fat were added in a proportion of 0.05% of the whole extract,
The mixture was stirred and mixed to obtain an extract liquid emulsion.

[0032]

[Example 11] The extract obtained in Example 10 was concentrated using an evaporator at an upper limit temperature of 45 ° C until the volume became 1/10 to obtain a paste-like concentrate. The solid content concentration of the paste-like concentrate was 13%.

[0033]

Example 12 The paste-like concentrate obtained in Example 11 was dried using a freeze dryer to obtain a dried product. The water content of the dried product was 0.4%.

Example products 1 to 12 thus obtained
For, the growth promoting effect of general lactic acid bacteria and bifidobacteria was evaluated. The results are shown in Tables 1 to 3 below. The growth promoting effect was evaluated according to the following method.

First, general lactic acid bacteria are Lactobacillus (L
Lactobacillus belonging to the genus actobacillus)
L. Casei, Lactobacillus bulgaricus, Lactobacillus
L. Plantaram, Lactobacillus faecalis, Lactobacillus acidophilus
s), Leuconostoc dextraninome (Leu.
Dextranicum), leuconostoc citrovolum (Leu. Citrovorum), Streptococcus thermophilus (St. Thermomo) belonging to the genus Streptococcus
philus) was used.

Bifidobacterium is a bacterium belonging to the genus Bifidobacterium, Bifidobacterium addresscentis (Bif.Ad).
olecentis), Bifidobacterium longum (Bif. Longum), and Bifidobacterium bifidum (Bif. Bifidum) were used.

Before evaluating the growth promoting effect, the above-mentioned general lactic acid bacteria and bifidobacteria were previously liquid-cultured and activated.

Cultivation of general lactic acid bacteria was performed according to the method described below. That is, the lactic acid bacteria proliferating agents of Example products 1 to 12 were mixed in a sterilized GYP liquid medium at the ratios shown in Tables 1 to 3 below, and 0.5% of the pre-activated general lactic acid bacteria was added.
The above-mentioned GYP liquid medium was inoculated separately for each strain at a concentration. The GYP liquid medium inoculated with the above-mentioned general lactic acid bacteria was cultured at 37 ° C. for 24 hours under aerobic conditions. What was cultured in this manner was used as an Example group. A control group was prepared by culturing the above-mentioned general lactic acid bacterium under the same conditions and operations using a sterilized GYP liquid medium containing no lactic acid bacterium-proliferating agent as an example product.

Cultivation of bifidobacteria was performed according to the method described below. That is, the lactic acid bacteria proliferating agents of Example products 1 to 12 were mixed in a sterilized BHI liquid medium at the ratios shown in Tables 1 to 3 below, and the pre-activated bifidobacteria were added to the sterilized BHI liquid medium at 0.
The above BHI liquid medium was inoculated separately at 5% concentration for each strain. The BHI liquid medium inoculated with the above Bifidobacterium was cultured under anaerobic conditions at 37 ° C. for 24 hours. What was cultured in this manner was used as an Example group. A control group was prepared by culturing the above-mentioned Bifidobacteria under the same conditions and operations using a sterilized GYP liquid medium containing no lactic acid bacterium-proliferating agent as an example product.

Next, the growth characteristics of the above-mentioned cultivated general lactic acid bacteria and bifidobacteria were determined by the following method for measuring the number of bacteria.

That is, in the case of the general lactic acid bacteria, the general lactic acid bacteria of the above-mentioned cultivated Example group and control group were BC so that the number of colonies in the petri dish was 200 to 500, respectively.
Appropriately diluted to P agar medium, transferred and solidified with the above BCP agar medium. Then, the cells were cultured at 37 ° C. for 3 days under aerobic conditions.

In the case of Bifidobacteria, the Bifidobacteria of the above-mentioned cultivated Example group and control group were subjected to BL so that the number of colonies in the petri dish was 200 to 500, respectively.
It was appropriately diluted and transferred to an agar medium and solidified with the BL agar medium. Then, the cells were cultured at 37 ° C. for 3 days under anaerobic conditions.

The growth characteristics were measured by measuring the number of colonies in the petri dish of the cultured general lactic acid bacteria and bifidobacteria in the BCP agar medium and the BL agar medium, and the number of colonies in the control group was 100. Colonies of ~ 200 +, ~ 500 ++, ~
1000 was ++++, 5,000 was ++++, and those exceeding 5000 were ++++. The number of colonies corresponds to the number of bacteria.

[0044]

[Table 1]

[0045]

[Table 2]

[0046]

[Table 3]

From the results shown in Tables 1 to 3 above, it is understood that the lactic acid bacteria-proliferating agents of all the Examples have a remarkable growth promoting effect on general lactic acid bacteria and bifidobacteria regardless of the bacterial species.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification number Office reference number FI technical display location // (C12N 1/20 C12R 1: 225) (C12N 1/20 C12R 1:46) (C12N 1 / 20 C12R 1:01) (72) Inventor Akira Nii 2-3-10, Hashiuchi, Ibaraki City, Osaka Prefecture (72) Inventor ▲ Haruhito Mura 14-28-3 Nakamura Town, Ibaraki City, Osaka Prefecture

Claims (13)

[Claims] 1. A lactic acid bacterium-proliferating agent comprising an extract extracted from leaves of a coffee genus plant as a main component. 2. The lactic acid bacterium growth agent according to claim 1, wherein the extract is dissolved in an extraction medium and is in a liquid or paste form. 3. The extract, which is in the form of liquid or paste,
The emulsifier is emulsified by adding an emulsifier and oil.
The lactic acid bacterium growth agent described. 4. The lactic acid bacterium growing agent according to claim 1, wherein the lactic acid bacterium growing agent has a water content of 1% by weight or less based on the whole lactic acid bacterium growing agent. 5. The lactic acid bacteria proliferating agent according to any one of claims 1 to 4, wherein the leaves of the coffee plant are non-fermented leaves. 6. The lactic acid bacteria proliferating agent according to claim 1, wherein the leaves of the plant of the genus Coffea are fermented leaves. 7. A method for producing a lactic acid bacterium growing agent, which comprises extracting leaves of a coffee tree plant with water. 8. A method for producing a lactic acid bacterium growing agent, which comprises concentrating an extract obtained by water-extracting leaves of a coffee plant of the genus Coffea to form a paste. 9. A process for producing a lactic acid bacterium-growing agent, which comprises adding an emulsifier and an oil and fat to an extract obtained by water-extracting leaves of a coffee genus plant and emulsifying the extract. 10. A process for producing a lactic acid bacterium growing agent, which comprises drying an extract obtained by extracting leaves of a coffee tree plant with water with water. 11. A method for producing a lactic acid bacterium growing agent, which comprises removing oil-soluble components of an extract obtained by water-extracting leaves of a coffee plant, and concentrating and solidifying the extract. 12. The method for producing a lactic acid bacterium growing agent according to claim 7, wherein non-fermented leaves are used as leaves of the plant of the genus Coffea. 13. The method for producing a lactic acid bacterium proliferating agent according to claim 7, wherein fermented leaves are used as leaves of the plant of the genus Coffee.