JPH06102604B2 - Disinfectant - Google Patents

Description

TECHNICAL FIELD The present invention relates to an alkylbenzyldimethylammonium salt,
The present invention relates to a bactericidal disinfectant containing a cetyltrimethylammonium halide or a cetylpyridinium halide in which adsorption of an alkylbenzyldimethylammonium salt on a polymer support is suppressed. This agent effectively exhibits sterilizing power even when it is brought into contact with an object in the presence of a polymer support.

2. Description of the Related Art Alkylbenzyldimethylammonium, such as chloride (also called benzalkonium chloride, hereinafter referred to as “ABDA
C) is an aqueous solution that is colorless and odorless, and has antibacterial properties not only against Gram-positive and negative bacteria but also against fungi, so it is useful as a germicidal disinfectant (Japan Pharmacopoeia, 10th revision, C-343, 1981, Hirokawa Shoten). In addition, cetyltrimethylammonium halide (eg, cetyltrimethylammonium bromide) and cetylpyridinium halide (eg, cetylpyridinium chloride) are also known as compounds having a bactericidal action (Kyosuke Tsuda, Hisashi Nogami; formulation design) Law (2), Jishin Shokan (Showa 46), page 429).

Problems to be Solved by the Invention Alkylbenzyldimethylammonium salts such as AB
Although the DAC aqueous solution is widely used as a sterilizing agent, when the cellulosic support such as absorbent cotton and gauze is soaked and sterilized by wiping, the sterilizing and disinfecting ability is reduced. A similar decrease in sterilization and disinfection ability is also observed in the case of a holder prepared from a synthetic polymer material such as rayon, polyurethane, silicone, etc. in addition to wool. Therefore, it is desired to develop a method for effectively exerting the bactericidal and disinfecting power of ABDAC even when the above-mentioned polymer support is used.

Means for Solving the Problems In view of the situation as described above, the present inventors have conducted various studies on the cause of the decrease in the above-mentioned sterilizing / disinfecting power, and ABDAC is adsorbed on the polymer material, and the sterilizing / disinfecting solution contains It was found that it was based on the decrease in the amount of ABDAC educt.

Therefore, the present inventors next conducted various studies on methods for suppressing the adsorption of the polymer carrier of ABDAC, and as a result, the polymer retention was carried out by allowing coexistence of cetyltrimethylammonium bromide or cetylpyridinium chloride in the ABDAC aqueous solution. It was found that the amount of ABDAC educts on contact with the body was increased, and further research was conducted to complete the present invention.

That is, the present invention comprises an alkylbenzyldimethylammonium salt and cetyltrimethylammonium halide or cetylpyridinium halide,
It is a germicidal disinfectant in which the adsorption of alkylbenzyldimethylammonium salt on a polymer support is suppressed.

In the germicidal disinfectant of the present invention, the alkylbenzyl dimethyl ammonium salt obtained by a conventional method is used, and chloride (ABDAC) is particularly advantageously used. Examples of the alkyl group constituting ABDAC include those having 8 to 18 carbon atoms.
These are all adsorbed on the polymer support, but in the present invention, among them, particularly effective for inhibiting adsorption of components having an alkyl group having a carbon number of 12 or 14 having strong sterilizing / disinfecting power, or for desorption. obtain.

Next, examples of the cetyltrimethylammonium halide include bromide and chloride, and bromide is particularly preferable. Further, as cetylpyridinium halide,
Examples thereof include chloride and bromide, and chloride is particularly preferably used.

The germicidal disinfectant of the present invention is about 0.3 parts by weight or more, preferably about 3 parts by weight or more, more preferably about 10 parts by weight of cetyltrimethylammonium halide or cetylpyridinium halide per 100 parts by weight of alkylbenzyldimethylammonium salt. It is prepared by containing the above components in a proportion of at least 1 part. By setting such an amount ratio,
It is effective in suppressing the adsorption of alkylbenzyldimethylammonium salt on the polymer support. When the amount of cetyltrimethylammonium halide or cetylpyridinium halide is greater than the above amount, the adsorption suppression effect becomes stronger, but usually the maximum state is reached at about 4,000 parts by weight, so this level is generally regarded as the maximum amount. Good.

The dosage form of the disinfectant of the present invention is not particularly limited, and may be prepared into a solid agent, a liquid agent, a spray agent or the like by a conventionally known method. In the case of a liquid agent or a spray agent, water, alcohol or the like can be used as a solvent.

Next, when using this germicidal disinfectant, the concentration of alkylbenzyldimethylammonium salt should be about 0.005-1%.
(Weight / volume) the concentration of cetyltrimethylammonium halide or cetylpyridinium halide is about
It may be brought into contact with an object to be sterilized in a solution state such that the concentration becomes 0.003 to 0.4% (weight / volume). As the solvent at this time, water, alcohol (eg, ethanol), an aqueous alcohol solution, or the like can be used, and may be appropriately selected in consideration of the type of the sterilization object.

The method of contacting the sterilization target can be a conventionally known method, for example, dipping, spraying, sprinkling,
It can be carried out by washing, cleaning and wiping. As the object, it can be widely applied to various uses for the purpose of eliminating microbial contamination. For example, medical treatment of affected parts of humans and animals or various medical instruments / facility, disinfection of fingers, barber / beauty salon, public facilities such as schools, houses, vehicles, tableware, food manufacturing factories or their manufacturing facilities. Can be used for.

In particular, the disinfectant of the present invention can be particularly advantageously used for an application method through a polymeric support such as a cellulosic material, polyethylene, polyester, polyurethane or silicone synthetic resin. As a specific example of the present application method, gauze, purified absorbent cotton, non-woven fabric, etc. are soaked in the sterilizing and disinfecting solution and used as a medicine for sterilizing or disinfecting fingers, disinfecting an affected area, sterilizing skin or mucous membrane, etc. , Absorbent cotton, purified absorbent cotton, non-woven fabric, towel, paper cotton,
As a quasi-drug for cleaning and cleaning the baby's skin and mouth, or cleaning and cleaning the nipple breast when breast-feeding and cleaning the eyes, local area and anus by impregnating a paper cloth etc. with this sterilizing solution The method used is given.

EXAMPLES The present invention will be described more specifically below with reference to experimental examples and examples.

Experimental Example 1 C ₁₂ H ₂₅ ABDAC, C ₁₄ H ₂₉ AB DAC or C ₁₂ H ₂₅ AB DAC and C ₁₄ H ₂₉ AB
In each aqueous solution containing 0.01% of the mixture of DAC (molar ratio of C ₁₂ H ₂₅ form and C ₁₄ H ₂₉ form 1: 0.54), purified absorbent cotton was added in g /
When soaked to 10 ml and left at 25 ° C. for 1 hour, the amount of these ABDAC adsorbed on the purified absorbent cotton reached an equilibrium. Next, cetyltrimethylammonium bromide (C ₁₆ TMAB) was added thereto at various concentrations as shown in FIG. 1, and the concentration of ABDAC in the aqueous solution was quantified by high performance liquid chromatography (HPLC). This quantitative method is based on Mayer [Journal of Pharmaceutical Sciences (J.Ph
arm.Sci. 69 ), 1148 (1980)] and improved Radial
Pak 5CN column ・ Detection wavelength λ254nm, 0.1M, pH5.0 Acetate buffer-acetonitrim (45:55), flow rate 2ml / min
By the method of quantifying ABDAC.

The relationship between the amount of C ₁₆ TMAB added and the amount of free form of each ABDAC is shown in FIG. As a result, the concentration of free ABCAC was already increased by the addition of 0.1 mmol / C ₁₆ TMAB, and the concentration of ABDAC was 95% by the addition of 5 mmol / AB.
Was released.

Experimental Example 2 C ₁₆ TMAB was added to an aqueous solution containing C ₁₂ H ₂₅ or C ₁₄ H ₂₉ ABDAC in an amount of 0.005 to 0.1% so that the concentration of C ₁₆ TMAB was 0 to 1 mmol / ml, to prepare an aqueous solution containing these at various ratios. Purified absorbent cotton was dipped in this aqueous solution so as to have a concentration of g / 10 ml, and the amount of the free form of each ABDAC after adsorption equilibrium was measured by the same method as in Experimental Example 1.

The results are shown in Fig. 2 for the case of C ₁₄ H ₂₉ AB DAC.

As is clear from FIG. 2, the higher the concentration of C ₁₆ TMAB, the higher the concentration of free ABDAC. For example, C ₁₆ TMAB 1 mmol /
Free C ₁₄ H ₂₉ ABDAC 45-at ABDAC concentration 0.005-0.1%
It showed 59%.

Experimental Example 3 C ₁₂ H ₂₅ ABDAC, C ₁₄ H ₂₉ AB DAC or C ₁₂ H ₂₅ AB DAC and C ₁₄ H ₂₉ AB
Cetylpyridinium chloride (C ₁₆ PC) was added to an aqueous solution containing 0.01% of a mixture of DAC (molar ratio of C ₁₂ H ₂₅ form and C ₁₄ H ₂₉ form of 1: 0.54) at 0 to 1 mmol /, Aqueous solutions containing these in various ratios were prepared. Purified absorbent cotton was dipped in this aqueous solution so as to have a concentration of g / 10 ml, and the amount of the free form of ABDAC after adsorption equilibrium was measured by the same method as in Experimental Example 1. The results are shown in FIG. As is clear from FIG. 3, the higher the C ₁₆ PC concentration, the higher the concentration of free ABDAC,
For example, with C ₁₆ PC 1 mmol /, at a C ₁₂ H ₂₅ ABDAC concentration of 0.01%, free C ₁₂ H ₂₅ ABDAC was 70%, free C ₁₄ H ₂₉ ABDAC was 37%, and the total amount of both educts was 59%.

Example 1 About 0.7 g of paper cotton (cloth) having a length of 10 cm and a width of 15 cm, ABDAC (C ₁₂ H ₂₅ AB
DAC: 67%, C ₁₄ H ₂₉ ABDAC: 29%, C ₁₆ H ₃₃ ABDAC: 5%) 0.1%, C
About 1 sterilizing and disinfecting aqueous solution containing ₁₆ TMAB 0.5 mmol /.
Impregnated with 6g, sealed with aluminum foil, sterilized for fingers,
Make a disinfectant.

Example 2 ABDAC (same composition as in Example 1) 0.02%, C ₁₆ PC 1 mmol
A sterilizing and disinfecting aqueous solution containing / is filled in a manual spray container to make a spray agent, and about 10.8 g of this liquid is added to a vertical 7.5c
After spraying about 2.7 g of absorbent cotton with m and width of 8 cm, put it in a packaging laminate film (polyethylene / polyester composite film) and heat seal. This is sterilized by high-pressure steam for 20 minutes at 121 ° C to produce a skin cleansing cotton.

Example 3 ABDAC on an approximately 4.2 g embossed paper towel measuring 17 cm in length and 28.5 cm in width
(Same composition as that of Example 1) 0.05%, C ₁₆ TMAB 0.2 mmol /
Approximately 7.1 g of a sterilizing and disinfecting aqueous solution containing is impregnated and sealed with a cellophane membrane to make a sterilizing and disinfecting paper napkin.

Effect of the Invention The sterilizing agent of the present invention can be widely applied to sterilizing various objects. In particular, even when it is used for sterilization through a polymer support, the sterilization power of the alkylbenzyldimethylammonium salt can be effectively exerted. That is, the coexistence of cetyltrimethylammonium halide or cetylpyridinium halide can prevent the alkylbenzyldimethylammonium salt from being adsorbed on the polymer support, whereby the alkylbenzyldimethylammonium salt in the solution can be suppressed. The amount of free substance is increased, and the purpose of sterilizing power can be fully achieved.

[Brief description of drawings]

Fig. 1 shows the amount of free form of ABDAC when C ₁₆ TMAB was added to the aqueous solution of ABDAC containing absorbent cotton. In the figure,-●-represents C
_In the case of ₁₂ H ₂₅ ABDAC, − ○ − is C − in the case of ₁₄ H ₂₉ ABDAC − ▲
− Indicates the case of the mixture of C ₁₂ H ₂₅ ABDAC and C ₁₄ H ₂₉ ABDAC, respectively. Fig. 2 shows the amount of each ABDAC in the free form when absorbent cotton was immersed in a coexisting aqueous solution of C ₁₄ H ₂₉ ABDAC and C ₁₆ TMAB.
In the figure, the curves indicated by the numbers 1, 2, 3, 4 and 5 are C
Shown is the case where ₁₆ TMAB was added at concentrations of 0, 0.1, 0.2, 0.5 and 1 mmol /, respectively. Figure 3 shows the amount of ABDAC in the free form when absorbent cotton was dipped in a coexisting aqueous solution of ABDAC and C ₁₆ PC. In the figure,-●-is C ₁₂ H
_In the case of ₂₅ ABDAC, − ○ − is C ₁₄ H _{29 In} the case of ABDAC, − ▲ − is
Shows the case of C ₁₂ H ₂₅ ABDAC and C ₁₄ H ₂₉ mixture of ABDAC respectively.

Claims (1)

[Claims] 1. A bactericidal disinfectant containing an alkyldimethylammonium salt and cetyltrimethylammonium halide or cetylpyridinium halide, in which adsorption of the alkyldimethylammonium salt to a polymer support is suppressed.