JPH0586184B2 - - Google Patents

Info

Publication number

JPH0586184B2

JPH0586184B2 JP20515586A JP20515586A JPH0586184B2 JP H0586184 B2 JPH0586184 B2 JP H0586184B2 JP 20515586 A JP20515586 A JP 20515586A JP 20515586 A JP20515586 A JP 20515586A JP H0586184 B2 JPH0586184 B2 JP H0586184B2

Authority

JP

Japan

Prior art keywords

bacillus

protein

strain

strains

proteins

Prior art date

1986-09-02

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Expired - Lifetime

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• 108090000623 proteins and genes Proteins 0.000 claims description 28
• 102000004169 proteins and genes Human genes 0.000 claims description 24
• 102000035195 Peptidases Human genes 0.000 claims description 10
• 108091005804 Peptidases Proteins 0.000 claims description 10
• 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 claims description 9
• 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 8
• 235000018102 proteins Nutrition 0.000 description 23
• 230000001580 bacterial effect Effects 0.000 description 17
• HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
• 241000193830 Bacillus <bacterium> Species 0.000 description 11
• 241000894006 Bacteria Species 0.000 description 11
• 239000002609 medium Substances 0.000 description 9
• 241000193764 Brevibacillus brevis Species 0.000 description 8
• 102000011632 Caseins Human genes 0.000 description 6
• 108010076119 Caseins Proteins 0.000 description 6
• 235000013336 milk Nutrition 0.000 description 6
• 239000008267 milk Substances 0.000 description 6
• 210000004080 milk Anatomy 0.000 description 6
• 241000894007 species Species 0.000 description 6
• 229920001817 Agar Polymers 0.000 description 5
• 239000008272 agar Substances 0.000 description 5
• 239000005018 casein Substances 0.000 description 5
• BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 5
• 235000021240 caseins Nutrition 0.000 description 5
• 244000005700 microbiome Species 0.000 description 5
• 244000063299 Bacillus subtilis Species 0.000 description 4
• 235000014469 Bacillus subtilis Nutrition 0.000 description 4
• 108090000637 alpha-Amylases Proteins 0.000 description 4
• 238000012258 culturing Methods 0.000 description 4
• 239000001963 growth medium Substances 0.000 description 4
• 238000004519 manufacturing process Methods 0.000 description 4
• 239000000463 material Substances 0.000 description 4
• 238000005259 measurement Methods 0.000 description 4
• 239000000047 product Substances 0.000 description 4
• 230000006798 recombination Effects 0.000 description 4
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
• 102000004139 alpha-Amylases Human genes 0.000 description 3
• 229940024171 alpha-amylase Drugs 0.000 description 3
• 229940098773 bovine serum albumin Drugs 0.000 description 3
• 239000002244 precipitate Substances 0.000 description 3
• 102000004196 processed proteins & peptides Human genes 0.000 description 3
• 108090000765 processed proteins & peptides Proteins 0.000 description 3
• 238000012545 processing Methods 0.000 description 3
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
• 238000003756 stirring Methods 0.000 description 3
• YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 3
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
• 241000588724 Escherichia coli Species 0.000 description 2
• 108050001049 Extracellular proteins Proteins 0.000 description 2
• 238000005119 centrifugation Methods 0.000 description 2
• 235000021245 dietary protein Nutrition 0.000 description 2
• 239000000706 filtrate Substances 0.000 description 2
• 235000013305 food Nutrition 0.000 description 2
• 235000009727 food gelling agent Nutrition 0.000 description 2
• 235000010855 food raising agent Nutrition 0.000 description 2
• 239000003349 gelling agent Substances 0.000 description 2
• 238000000227 grinding Methods 0.000 description 2
• 239000012770 industrial material Substances 0.000 description 2
• 239000002649 leather substitute Substances 0.000 description 2
• 238000000034 method Methods 0.000 description 2
• 239000000123 paper Substances 0.000 description 2
• VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
• 239000013612 plasmid Substances 0.000 description 2
• BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
• 239000006228 supernatant Substances 0.000 description 2
• HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
• 241000304886 Bacilli Species 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• 238000003794 Gram staining Methods 0.000 description 1
• 102000014150 Interferons Human genes 0.000 description 1
• 108010050904 Interferons Proteins 0.000 description 1
• 239000001888 Peptone Substances 0.000 description 1
• 108010080698 Peptones Proteins 0.000 description 1
• 239000004365 Protease Substances 0.000 description 1
• 238000005273 aeration Methods 0.000 description 1
• 238000009835 boiling Methods 0.000 description 1
• 229940041514 candida albicans extract Drugs 0.000 description 1
• 238000007796 conventional method Methods 0.000 description 1
• NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
• 239000012228 culture supernatant Substances 0.000 description 1
• 239000003814 drug Substances 0.000 description 1
• 230000000694 effects Effects 0.000 description 1
• 239000000284 extract Substances 0.000 description 1
• 239000008103 glucose Substances 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 229940079322 interferon Drugs 0.000 description 1
• 238000002955 isolation Methods 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 235000013372 meat Nutrition 0.000 description 1
• 230000000813 microbial effect Effects 0.000 description 1
• 230000000877 morphologic effect Effects 0.000 description 1
• 235000019319 peptone Nutrition 0.000 description 1
• 230000001766 physiological effect Effects 0.000 description 1
• 229910052697 platinum Inorganic materials 0.000 description 1
• 239000000843 powder Substances 0.000 description 1
• 239000013587 production medium Substances 0.000 description 1
• 238000002331 protein detection Methods 0.000 description 1
• 230000003248 secreting effect Effects 0.000 description 1
• 230000028327 secretion Effects 0.000 description 1
• 235000020183 skimmed milk Nutrition 0.000 description 1
• 239000002689 soil Substances 0.000 description 1
• 238000010186 staining Methods 0.000 description 1
• 230000001954 sterilising effect Effects 0.000 description 1
• 238000004659 sterilization and disinfection Methods 0.000 description 1
• 238000004381 surface treatment Methods 0.000 description 1
• 238000012360 testing method Methods 0.000 description 1
• 230000014616 translation Effects 0.000 description 1
• 239000012138 yeast extract Substances 0.000 description 1
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1