JPH0585156B2 - - Google Patents
Info
- Publication number
- JPH0585156B2 JPH0585156B2 JP60258269A JP25826985A JPH0585156B2 JP H0585156 B2 JPH0585156 B2 JP H0585156B2 JP 60258269 A JP60258269 A JP 60258269A JP 25826985 A JP25826985 A JP 25826985A JP H0585156 B2 JPH0585156 B2 JP H0585156B2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- medium
- cells
- culture dish
- metal filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002609 medium Substances 0.000 claims description 27
- 239000001963 growth medium Substances 0.000 claims description 21
- 239000002184 metal Substances 0.000 claims description 19
- 238000012258 culturing Methods 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 description 25
- 238000000034 method Methods 0.000 description 19
- 239000007789 gas Substances 0.000 description 7
- 239000011521 glass Substances 0.000 description 7
- 239000012510 hollow fiber Substances 0.000 description 7
- 239000011324 bead Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000001464 adherent effect Effects 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 4
- 239000000919 ceramic Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000011325 microbead Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60258269A JPS62118878A (ja) | 1985-11-18 | 1985-11-18 | 細胞培養装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60258269A JPS62118878A (ja) | 1985-11-18 | 1985-11-18 | 細胞培養装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62118878A JPS62118878A (ja) | 1987-05-30 |
| JPH0585156B2 true JPH0585156B2 (ko) | 1993-12-06 |
Family
ID=17317890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60258269A Granted JPS62118878A (ja) | 1985-11-18 | 1985-11-18 | 細胞培養装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62118878A (ko) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5443985A (en) * | 1993-07-22 | 1995-08-22 | Alberta Research Council | Cell culture bioreactor |
| JPWO2005059091A1 (ja) * | 2003-12-18 | 2007-12-13 | 株式会社日立メディコ | 細胞培養装置 |
| JP4970823B2 (ja) * | 2006-03-30 | 2012-07-11 | 株式会社 ジャパン・ティッシュ・エンジニアリング | 培養組織用トレイ |
-
1985
- 1985-11-18 JP JP60258269A patent/JPS62118878A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62118878A (ja) | 1987-05-30 |
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