JPH058385B2 - - Google Patents
Info
- Publication number
- JPH058385B2 JPH058385B2 JP59062082A JP6208284A JPH058385B2 JP H058385 B2 JPH058385 B2 JP H058385B2 JP 59062082 A JP59062082 A JP 59062082A JP 6208284 A JP6208284 A JP 6208284A JP H058385 B2 JPH058385 B2 JP H058385B2
- Authority
- JP
- Japan
- Prior art keywords
- different
- dyes
- optical stimulation
- region
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000004027 cell Anatomy 0.000 claims description 73
- 239000000975 dye Substances 0.000 claims description 66
- 230000003287 optical effect Effects 0.000 claims description 43
- 230000005284 excitation Effects 0.000 claims description 30
- 230000000638 stimulation Effects 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 21
- 238000002372 labelling Methods 0.000 claims description 14
- 239000000427 antigen Substances 0.000 claims description 13
- 102000036639 antigens Human genes 0.000 claims description 13
- 108091007433 antigens Proteins 0.000 claims description 13
- 238000001514 detection method Methods 0.000 claims description 13
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 11
- 239000007850 fluorescent dye Substances 0.000 claims description 11
- 238000000295 emission spectrum Methods 0.000 claims description 9
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 7
- 229910052786 argon Inorganic materials 0.000 claims description 7
- -1 argon ion Chemical class 0.000 claims description 7
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 7
- 230000003595 spectral effect Effects 0.000 claims description 7
- 108010004729 Phycoerythrin Proteins 0.000 claims description 6
- CPBQJMYROZQQJC-UHFFFAOYSA-N helium neon Chemical compound [He].[Ne] CPBQJMYROZQQJC-UHFFFAOYSA-N 0.000 claims description 2
- 108010004469 allophycocyanin Proteins 0.000 claims 3
- 230000002596 correlated effect Effects 0.000 claims 2
- 239000000049 pigment Substances 0.000 claims 2
- 101710160107 Outer membrane protein A Proteins 0.000 claims 1
- 108010053210 Phycocyanin Proteins 0.000 claims 1
- 239000002245 particle Substances 0.000 description 61
- 238000000684 flow cytometry Methods 0.000 description 23
- 239000000523 sample Substances 0.000 description 22
- 238000000149 argon plasma sintering Methods 0.000 description 14
- 239000003795 chemical substances by application Substances 0.000 description 8
- 210000000265 leukocyte Anatomy 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 7
- 230000004888 barrier function Effects 0.000 description 5
- 238000010166 immunofluorescence Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000011002 quantification Methods 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 230000000890 antigenic effect Effects 0.000 description 4
- 238000004163 cytometry Methods 0.000 description 4
- 238000000695 excitation spectrum Methods 0.000 description 4
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 3
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 3
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- 108090001008 Avidin Proteins 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 230000001427 coherent effect Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 210000001616 monocyte Anatomy 0.000 description 2
- 210000002433 mononuclear leukocyte Anatomy 0.000 description 2
- 238000007447 staining method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 108060006184 phycobiliprotein Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000012950 reanalysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000001429 visible spectrum Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Landscapes
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
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Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US48234583A | 1983-04-05 | 1983-04-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59184862A JPS59184862A (ja) | 1984-10-20 |
| JPH058385B2 true JPH058385B2 (ja) | 1993-02-02 |
Family
ID=23915688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59062082A Granted JPS59184862A (ja) | 1983-04-05 | 1984-03-29 | è©Šæå 现èã®è€æ°å¯æ¬¡éå£ãèå¥ããæ¹æ³ãšè£ 眮 |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0121262B1 (ja) |
| JP (1) | JPS59184862A (ja) |
| DE (1) | DE3485290D1 (ja) |
| MY (1) | MY102914A (ja) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4727020A (en) * | 1985-02-25 | 1988-02-23 | Becton, Dickinson And Company | Method for analysis of subpopulations of blood cells |
| JPS61225656A (ja) * | 1985-03-29 | 1986-10-07 | Toshiba Corp | æ€äœæ€æ»è£ 眮 |
| US5206143A (en) * | 1985-11-01 | 1993-04-27 | Smithkline Beecham Corporation | Method and reagents for performing subset analysis using quantitative differences in fluorescence intensity |
| US4710635A (en) * | 1986-04-14 | 1987-12-01 | Becton, Dickinson And Company | Dual laser excitation from single laser source |
| US4704891A (en) * | 1986-08-29 | 1987-11-10 | Becton, Dickinson And Company | Method and materials for calibrating flow cytometers and other analysis instruments |
| JPH076979B2 (ja) * | 1986-09-10 | 1995-01-30 | æ±äºå»çšé»åæ ªåŒäŒç€Ÿ | ããâãµã€ãã¡ããªâã«ããçœè¡çåé¡ã«äœ¿çšãããè©Šè¬ |
| EP0266881A3 (en) * | 1986-09-30 | 1990-04-04 | Astromed Limited | Method and apparatus for multiple optical assaying |
| US4882284A (en) * | 1987-04-13 | 1989-11-21 | Ortho Pharmaceutical Corporation | Method for quantitating and differentiating white blood cells |
| SE458968B (sv) * | 1987-06-16 | 1989-05-22 | Wallac Oy | Biospecifikt analysfoerfarande foer flera analyter i vilket ingaar partikelraekning och maerkning med fluorescerande maerksubstanser |
| US5057413A (en) * | 1988-06-13 | 1991-10-15 | Becton, Dickinson And Company | Method for discriminating between intact and damaged cells in a sample |
| FI82144C (fi) * | 1989-03-22 | 1991-01-10 | Wallac Oy | Foerfarande foer samtidig bestaemning av flera ligander. |
| ES2112211B1 (es) * | 1996-03-26 | 1998-12-16 | Univ Salamanca | Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas. |
| WO2003083112A1 (fr) * | 2002-04-01 | 2003-10-09 | Japan Science And Technology Agency | Population de cellules pourvues de codes d'identification et procedes pour le criblage de population de cellules |
| JP3891925B2 (ja) | 2002-12-03 | 2007-03-14 | ãã€ãã€ãªãµã€ãšã³ã¹æ ªåŒäŒç€Ÿ | çç©åŠçç²åã®æ å ±ãåŸãè£ çœ® |
| US7326573B2 (en) * | 2003-01-10 | 2008-02-05 | Beckman Coulter, Inc. | Assay procedures and apparatus |
| JP4304120B2 (ja) | 2004-04-30 | 2009-07-29 | ãã€ãã€ãªãµã€ãšã³ã¹æ ªåŒäŒç€Ÿ | çç©åŠçç²åããœãŒãã£ã³ã°ããè£ çœ®åã³æ¹æ³ |
| US8436999B2 (en) | 2007-09-28 | 2013-05-07 | Illumina, Inc. | Fluorescence excitation and detection system and method |
| CN110308127A (zh) * | 2019-08-15 | 2019-10-08 | åå·æŽæŸå»çç§ææéå ¬åž | å¯å€è§å ä¿¡å·åæ¶æ£æµçå åŠåæè£ çœ® |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3864571A (en) * | 1971-02-10 | 1975-02-04 | Wheeler International Inc | Method and Apparatus for Automatically, Identifying and Counting Various Cells in Body Fluids |
| US3916205A (en) * | 1973-05-31 | 1975-10-28 | Block Engineering | Differential counting of leukocytes and other cells |
| US3916197A (en) * | 1973-11-28 | 1975-10-28 | Particle Technology Inc | Method and apparatus for classifying biological cells |
| US4284412A (en) * | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
-
1984
- 1984-03-29 JP JP59062082A patent/JPS59184862A/ja active Granted
- 1984-03-31 DE DE8484103603T patent/DE3485290D1/de not_active Expired - Fee Related
- 1984-03-31 EP EP84103603A patent/EP0121262B1/en not_active Expired - Lifetime
-
1987
- 1987-09-29 MY MYPI87002128A patent/MY102914A/en unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3864571A (en) * | 1971-02-10 | 1975-02-04 | Wheeler International Inc | Method and Apparatus for Automatically, Identifying and Counting Various Cells in Body Fluids |
| US3916205A (en) * | 1973-05-31 | 1975-10-28 | Block Engineering | Differential counting of leukocytes and other cells |
| US3916197A (en) * | 1973-11-28 | 1975-10-28 | Particle Technology Inc | Method and apparatus for classifying biological cells |
| US4284412A (en) * | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0121262A2 (en) | 1984-10-10 |
| JPS59184862A (ja) | 1984-10-20 |
| EP0121262B1 (en) | 1991-11-27 |
| DE3485290D1 (de) | 1992-01-09 |
| MY102914A (en) | 1993-03-31 |
| EP0121262A3 (en) | 1985-11-06 |
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