JPH0571757U - Concentrating cell for ultra-trace sample spectrophotometry and its holder - Google Patents

Abstract

(57) [Summary] (Correction) [Purpose] Spectroscopic analysis of ultra-trace amounts of samples is possible, cells can be easily replaced, and more accurate measurements can be made compared to conventional methods. [Structure] In a condenser holder having condenser lenses on the left and right sides, a rectangular condenser cell having a rectangular fine hole penetrating from the upper part to the lower part, and the upper and lower openings of the fine hole are widened toward the center, Condensation cell for ultra-trace sample spectrophotometry and its holder, which is held by a hole in the upper part and a countersink in the lower part.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention relates to a spectrophotometric cell and a light-condensing cell holder suitable for spectrophotometric measurement of a small amount of sample in the fields of medicine and physics and chemistry.

[0002]

[Prior Art]

 Conventionally, a cell has been used for measuring spectrophotometry, but it is unsuitable for measuring a sample that has a large volume and only a small amount can be prepared.

In addition, as a measurement of the spectrophotometric value, after inserting a sample into a circular capillary and condensing light which has been focused to some extent by a focus beam type spectrophotometer through a lens and passing the sample in the circular capillary, There is a circular capillary method that allows the detector (Photomul) to detect the original size of the light through the opposing lens and the focusing lens of the spectroscope.

[0004]

[Problems to be solved by the device]

 However, the current method using the spectrophotometer for the focus beam has a drawback that the measurement accuracy is poor because the spectral beam hits the curved surface and causes surface reflection or scattering because the capillary has a circular shape.

Therefore, there has been a demand for the development of a spectrophotometric cell capable of measuring an extremely small amount of sample with high accuracy and easily exchanging the cell.

[0006]

[Means for Solving the Problems]

 Therefore, as a result of earnest studies to solve these conventional problems, the present inventor used a capillary cell of a specific shape instead of the circular capillary and used this cell as a holder equipped with a condenser lens. The present invention has been completed based on the knowledge that the above problems can be solved by detachably attaching, and spectroscopic measurement of a small amount of sample can be easily and highly accurately performed and the cell can be freely exchanged.

That is, the first invention is a light-collecting cell for ultra-trace sample spectrophotometric measurement, which has pores penetrating from the upper part to the lower part and spreads toward upper and lower openings of the pores.

A second invention is a condenser lens holder in which a central capillary cell is held by a hole in the upper part of the central portion and a counterbore in the lower portion, and condenser lenses are provided on the left and right sides.

Further, in the third invention, a condenser holder having condenser lenses 3 and 3'on the left and right has a fine hole 6 penetrating from the upper part to the lower part, and the upper and lower openings of the fine hole are It is a holder with a condenser cell for ultra-trace sample spectrophotometry, in which the condenser cell diverging toward the openings 7 and 7'is held by a hole in the upper part of the center and a countersink in the lower part.

Hereinafter, the present invention will be described with reference to the drawings, but the present invention is not limited thereto.

The capillary cell 1 of the present invention has a prismatic shape, has pores 6 penetrating from the upper portion to the lower portion, and spreads toward upper and lower openings 7, 7 ′ of the pores 6 ( Funnel processing).

As for the shape of the capillary cell 1, the circular capillary used in the conventional microanalysis has a curved shape, and the surface reflection or scattering on the curved surface is large. It is necessary that the receiving surface is a rectangular shape that is a plane perpendicular to the spectral light.

The pores 6 need to be thin so that the injected sample moves due to a capillary phenomenon, and the diameter varies depending on the sample to be injected, but normally 0.25 to 0.1 mm square is preferable. Further, the shape of the pores is required to be square for the same reason as above.

The upper part of the pores is funneled to facilitate injection of the sample, and the lower part of the sample is filled with capillarity in the pores to stop the sample due to surface tension. Finennel processing is applied to prevent leakage.

As the material of the cell, synthetic quartz glass, fused quartz glass, anhydrous fused quartz glass, optical glass, white plate glass, Pyrex glass, polystyrene material or the like is used.

The condensing cell holder 2 holds the rectangular capillary cell 1 in the central part, and condensing lenses 3 and 3 ′ are provided on the left and right sides of the central part and the object, and between the condensing lens and the cell. It is open. At this time, the distance between the condenser lens and the capillary is appropriately set according to the specifications of the measuring device.

Each of the condenser lenses 3 and 3 ′ is a convex lens whose inner surface is a hemispherical surface, and the outside of the holder is molded flat. Further, the angular capillary cell 1 is inserted by an upper hole provided at the center of the holder and held by a lower countersink 5. The insertion hole and the countersink are appropriately determined depending on the shape of the capillary.

Although the entire material of the light collecting cell holder 2 is made of synthetic quartz glass, it is also a preferable mode to perform masking using black glass except for the right and left collecting lens surfaces.

[0019]

[Action]

 As shown in FIG. 2, the spectroscopic light is focused by a focus lens installed in the apparatus, passes through a condenser lens provided in the cell holder, and is accurately condensed on the sample in the rectangular capillary. After that, the light that has passed through the sample passes through the optical system of the condenser lens and the focus lens, is returned to the beam diameter again, and is detected by the photomultiplier. The focus lens adjusts the focusing distance according to the wavelength.

Such a condenser lens holder is used because the measuring portion of the capillary is a fine hole and its range is small, so that it is necessary to narrow down the focus with higher precision. Since it requires a considerable focal length to narrow down the beam diameter by itself, the use of a holder with a lens integrated with the capillary shortens the focal length and enables highly accurate measurement.

[0021]

【effect】

 A very small amount of sample of a few μl can be measured with high accuracy, and the cell can be easily exchanged. By using a holder with a lens integrated with the capillary, the focal length can be shortened and the sample can be collected accurately. It is exposed to light and enables highly accurate measurement. Further, it becomes possible to perform fluorescence measurement in which 90 ° light is captured and measured.

[Brief description of drawings]

FIG. 1 is a perspective view showing a state in which a rectangular cell is held by a light collecting holder of the present invention.

FIG. 2 is a conceptual diagram showing the action of spectroscopic light passing through a condenser holder of the present invention.

FIG. 3 is a cross-sectional view of a capillary.

[Explanation of symbols]

 1 Capillary 2 Condensing Holder 3, 3'Condensing Lens 4, 4 'Focus Lens 5 Zakuri 6 Pore 7 Opening

Claims (5)

[Scope of utility model registration request] 1. Pore 6 penetrating from the upper part to the lower part,
A condensing cell for measuring an ultratrace amount of spectrophotometric light, which has a divergent shape toward the openings 7 and 7'above and below the pores. 2. The light-collecting cell for ultra-trace sample spectrophotometry according to claim 1, wherein the shape of the cell is square and the shape of the pores inside is also square. 3. A condenser lens holder which holds a rectangular capillary cell 1 by means of a hole in the upper part of the central part and a counterbore in the lower part and is provided with condensing lenses 3, 3'on the left and right. 4. The condenser lens holder according to claim 3, wherein black glass is used in addition to the condenser lens surface. 5. A condenser holder having condenser lenses 3 and 3'on the left and right has a pore 6 penetrating from the upper part to the lower part, and the upper and lower openings of the pore are openings 7 and 7 '. A holder with a condensing cell for ultra-trace sample spectrophotometry, which holds the condensing cell that widens toward the end with a hole in the upper part of the center and a countersink in the lower part.