JPH05271013A - Insect pest controlling agent comprising cyclic depsipeptide substance as active ingredient - Google Patents

Abstract

PURPOSE:To obtain an insect pest controlling agent in the fields of agriculture, horticulture, forestry and environmental sanitation. CONSTITUTION:The objective insect pest controlling agent comprising a substance PF1022 which is a cyclic depsipeptide substance expressed by the formula as an active ingredient. This controlling agent has insecticidal effects on noxious insects and nematodes in the fields of agriculture, horticulture, forestry and environmental sanitation.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a pest control agent containing a PF1022 substance, which is a cyclic depsipeptide substance, as an active ingredient.

[0002]

2. Description of the Related Art The PF1022 substance, which is a cyclic depsipeptide substance according to the present invention, is a substance having the following chemical structural formula (I) and has an anthelmintic action (JP-A-3-35796).

[0003]

[Chemical 1]

The anthelmintic agents in the above publications are animal parasites such as bovine and sheep torsion gastropods, ostertag gastric caterpillars, caterpillars,
Cooper nematode, intestinal tuberculosis, double mouth fluke, Benedin tapeworm,
Lungworms, liver flukes, swine roundworms, whipworms, intestinal tuberculosis, dog roundworms, hookworms, whipworms, filamentous insects, cat roundworms, Manson's cephalopodis, etc. It is stated that an effect is expected on endoparasites such as caecum, human roundworm, pinworm, hookworm, oriental hairy nematode, dung nematode, whipworm and the like. However, there is no description of use for insects or plant parasitic nematodes, which are major pests in the fields of agriculture and horticulture and environmental hygiene.

The anthelmintic effect described in the above publications is due to the worming effect based on the paralytic effect described in the specification, and piperazine and morantel, which are characterized by the similar motor paralytic effect of parasites, are excellent anthelmintic agents. Nonetheless, destomycin, which has no controlling effect on insects, or has an excellent anthelmintic effect on roundworms such as pigs, does not show a controlling effect on P. chinensis. From this, PF102
The discovery of the present invention that the two substances have excellent control effects against these pests is a surprising finding that cannot be easily analogized.

[0006]

In recent years, pests with developed resistance have emerged as a result of the emergence of pests that have developed resistance due to the long-term use of typical insecticides such as organophosphates, carbamates, and pyrethroids. There is a demand for the development of a new pest control agent that exhibits an excellent control effect and has low toxicity and low residue.

[0007]

As a result of intensive studies to solve the above problems, the present inventors have found that the PF1022 substance has an excellent pest control effect, and have completed the present invention. It was. That is, the gist of the present invention relates to a pest control agent containing a PF1022 substance which is a cyclic depsipeptide substance as an active ingredient.

The present invention will be described in detail below. The PF1022 substance which is the active ingredient of the present invention is described in the above-mentioned JP-A-3-35.
796, which is a cyclic depsipeptide, and is mainly a non-sporeless bacterium PF1022 strain (FERM BP-2.
671) is cultivated under aerobic conditions and extracted from the culture,
It can be produced by purification.

In carrying out the pest control agent of the present invention, the PF1022 substance can be used as it is,
Mix with various liquid carriers or solid carriers, add appropriate amount of auxiliary agent such as surfactant, and formulate into wettable powder, emulsion, aqueous liquid, oily liquid, powder, granule, bait or poison bait etc. To use. In that case, the active ingredient is PF102.
Only two substances may be used, but other insecticides, fungicides, herbicides,
It is more effective if mixed with an attractant, synergist, etc.
In addition, the purpose of pest control can be achieved labor-savingly.

The pest control agent of the present invention can be used by spraying it directly to a crop for which pest control is desired or by injecting it into a plant. In addition, it can be applied to the growing environment of crops such as the surface of water or the surface of soil, if necessary, and can also be mixed with soil for use. Alternatively, the pest can be directly ingested as poison bait.

When the pest control agent of the present invention is used as a spraying agent, the PF1022 substance is usually added to the spraying solution in an amount of 0.
It is desirable to be contained at a concentration of 1 to 1000 ppm, and when used by being mixed in an injectable or feed, it is desirable to be contained at a concentration of 0.01 to 500 ppm. Further, in the case of spraying in a concentrated small amount, since the PF1022 substance according to the present invention has little toxicity to warm-blooded animals, it can be used effectively and safely at a higher concentration.

[0012]

INDUSTRIAL APPLICABILITY According to the present invention, an excellent pest control agent having low toxicity can be provided.

[0013]

EXAMPLES Next, the effects of the pest control agent of the present invention will be clarified by the following test examples, and formulation examples will also be shown. Test Example 1 Efficacy test against silkworm larvae A third-instar larvae of silkworm was tested for 1 group and 10 larvae, and the insects were released in a plastic cup with a diameter of 9 cm containing an artificial feed mixed so that the PF1022 substance had a predetermined concentration at 25 ° C. It was kept in a constant temperature room. The number of live and dead insects after 6 days was investigated, and the mortality rate was calculated by the following formula. The results are shown in Table 1. Mortality rate (%) = (number of dead insects / number of released insects) x 100

[0014]

[Table 1] Table 1 Reagents Concentration of added mortality rate (PPM) (%) PF1022 substance 100 30 PF1022 substance 500 100 No additive 0 0

Test Example 2 Efficacy test against Larvae of Spodoptera litura, 20 mg of PF1022 substance was dissolved in 5 ml of acetone,
Diluted by adding 10 ml of water. This diluent is 9 cm in diameter
1/4 pieces of the dried artificial feed (Vitasilk, manufactured by Kyodo Feed Co., Ltd.) placed in the plastic cup of No. 1 was added to be sufficiently absorbed. After evaporating acetone at room temperature, 5 third-instar larvae of Spodoptera litura were released and were bred in a thermostatic chamber at 25 ° C. The number of live and dead insects after 5 days was investigated, and the mortality rate was calculated by the following formula. The mortality was 80%. The test is 2
Repeated. Mortality rate (%) = (number of dead insects / number of released insects) x 100

Test Example 3 Efficacy test against pine wood nematode A pine wood nematode cultivated on a Czapek agar medium previously inoculated with Botrytis cinerea was used as a test. The chemicals were prepared by dissolving and diluting a dry-heat sterilized cotton ball having a diameter of 5 mm to a predetermined concentration and treating it with 0.1 ml. Czape
k agar inoculated with Botrytis cinerea and cultured for 4 days Diameter 9
Place a chemically treated cotton ball in the center of a cm plastic dish, and add 0.1 ml (1 ml) of a pinewood nematode suspension.
(500 heads) were inoculated and cultured in the dark at 26 ° C. The pine wood nematodes that survived 5 days were separated by the Bertmann method, the number of surviving worms was counted and examined under a microscope, and the growth inhibition rate was determined by the following formula. The results are shown in Table 2. Growth inhibition rate (%) = (1-number of surviving insects in treated group / number of surviving insects in untreated group) × 100

[0017]

[Table 2] Table 2 Test drug Agent Drug treatment amount Growth inhibition rate (μg / cotton ball) (%) PF1022 substance 5 45 No additive 0 0

Formulation Example 1 1.25 parts by weight of PF1022 substance was dissolved in 50 parts by weight of N-methylpyrrolidone, and then 12.5 parts by weight of polyoxyethylene alkyl ether and 12.5 parts by weight of propylene glycol fatty acid ester were added. Dissolved.
Further, 23.75 parts by weight of water was added and mixed with stirring to prepare an aqueous liquid preparation.

Formulation Example 2 10 parts by weight of PF1022 substance, 10 parts by weight of polyoxyethylene alkylaryl ether, 5 parts by weight of alkylbenzene sulfonate and 75 parts by weight of clay were pulverized and mixed to prepare a wettable powder.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Tadaaki Okada 760 Shimooka-cho, Kohoku-ku, Yokohama-shi, Kanagawa Meiji Confectionery Co., Ltd.

Claims (1)

[Claims] 1. A cyclic depsipeptide substance, PF10.
A pest control agent, which comprises 22 substances as active ingredients.