JPH05219940A - Process for culturing photosynthetic microorganism and unit therefor - Google Patents
Process for culturing photosynthetic microorganism and unit thereforInfo
- Publication number
- JPH05219940A JPH05219940A JP4777992A JP4777992A JPH05219940A JP H05219940 A JPH05219940 A JP H05219940A JP 4777992 A JP4777992 A JP 4777992A JP 4777992 A JP4777992 A JP 4777992A JP H05219940 A JPH05219940 A JP H05219940A
- Authority
- JP
- Japan
- Prior art keywords
- light
- culture
- tank
- culturing
- ultraviolet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012258 culturing Methods 0.000 title claims abstract description 23
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 23
- 244000005700 microbiome Species 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000001954 sterilising effect Effects 0.000 claims abstract description 18
- 239000013307 optical fiber Substances 0.000 claims abstract description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 14
- 230000003287 optical effect Effects 0.000 claims description 8
- 239000012780 transparent material Substances 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 7
- 241000894006 Bacteria Species 0.000 abstract description 5
- 241000195493 Cryptophyta Species 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 description 16
- 239000000126 substance Substances 0.000 description 13
- 239000000243 solution Substances 0.000 description 11
- 239000007789 gas Substances 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、光合成微生物の培養方
法及び装置に係り、特に光合成細菌及び／又は微細藻類
などの光合成微生物を高速、高効率に培養する方法及び
装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method and apparatus for culturing photosynthetic microorganisms, and more particularly to a method and apparatus for culturing photosynthetic microorganisms such as photosynthetic bacteria and / or microalgae at high speed and with high efficiency.
【０００２】[0002]
【従来の技術】従来、光合成微生物の培養を目的とする
培養装置は、そのほとんどが装置の外壁から棒状の蛍光
燈等により光を槽内液に供給するか、あるいは装置内部
の中心に棒状の蛍光燈等を取付け、槽内液に光を供給す
るものが主流となっていた。しかしながら、最近では各
種の技術が研究開発され、その代表的な技術として太陽
光及び／又は人工光を光ファイバーで伝送し、伝送され
た光をさらに特殊な面発光ファイバーによって槽内液に
拡散するか、又は樹脂性の棒状ロッドに長さ方向に散乱
溝を設け槽内に縦方向に、かつ線状に光を拡散して光合
成微生物を培養する装置が開発されている。以上のよう
に、種々の光合成微生物の培養装置が存在するが、従来
の培養装置には次に列挙するような技術的問題点があ
る。2. Description of the Related Art Conventionally, most of the culturing devices intended for culturing photosynthetic microorganisms either supply light from the outer wall of the device to a liquid in a tank with a rod-shaped fluorescent lamp or a rod-shaped center at the center of the device. The mainstream method is to attach a fluorescent lamp to supply light to the liquid in the tank. However, recently, various technologies have been researched and developed, and as a typical technology, sunlight and / or artificial light is transmitted by an optical fiber, and the transmitted light is diffused into a liquid in a tank by a special surface emitting fiber. Alternatively, a device for culturing a photosynthetic microorganism by providing a scattering groove in a longitudinal direction on a resin rod-shaped rod and diffusing light linearly and linearly in a tank has been developed. As described above, there are various culturing devices for photosynthetic microorganisms, but the conventional culturing devices have the following technical problems.
【０００３】（１）培養槽の槽外に光源を配置して光エ
ネルギーを供給する培養装置は、槽を高温・高圧で滅菌
することができるが、伝達できる光エネルギー量が小さ
く、光合成微生物の大量培養には不利である。 （２）槽内に光ファイバーで光エネルギーを伝送する方
式は、伝送光エネルギー量が槽外方式よりもはるかに大
きく光合成微生物の培養に適しているが、従来の合成高
分子素材の光拡散体は耐熱性がないため直接蒸気滅菌が
できない。このため培養槽内にガラス製の保護管を設け
光拡散体が槽内液に直接接触することを防いでいるが、
光拡散体と槽内液との間に複数の界面ができるため光の
伝達効率が悪化する。また多数のガラス製保護管が培養
槽内に挿入されているため、維持管理が極めて煩雑であ
る。 （３）ガラス製保護管なしで合成高分子素材の光拡散体
を直接槽内液に挿入する場合は、薬液滅菌をする必要が
あるが、薬液が残留すると微生物の生育に悪影響を及ぼ
すためこれを洗浄するのに大量の滅菌水を要する。 （４）蒸気滅菌を行う場合は、蒸気発生装置を必要と
し、さらに培養槽及び配管も耐熱・耐圧構造にする必要
がある。(1) A culture device for supplying light energy by arranging a light source outside the culture tank can sterilize the tank at high temperature and high pressure, but the amount of light energy that can be transmitted is small, so that the photosynthetic microorganisms can be cultivated. It is disadvantageous for large-scale culture. (2) The method of transmitting light energy to the inside of the tank with an optical fiber is much larger than the method outside the tank and is suitable for culturing photosynthetic microorganisms. Direct steam sterilization is not possible due to lack of heat resistance. For this reason, a glass protective tube is provided in the culture tank to prevent the light diffuser from directly contacting the liquid in the tank.
Since a plurality of interfaces are formed between the light diffuser and the liquid in the tank, the light transmission efficiency is deteriorated. Moreover, since many glass protective tubes are inserted in the culture tank, maintenance is extremely complicated. (3) When the synthetic polymer light diffuser is directly inserted into the liquid in the tank without a glass protective tube, it is necessary to sterilize the liquid chemical, but if the liquid chemical remains, it adversely affects the growth of microorganisms. A large amount of sterilized water is required to wash the. (4) When performing steam sterilization, a steam generator is required, and the culture tank and piping must also have a heat-resistant and pressure-resistant structure.
【０００４】[0004]
【発明が解決しようとする課題】以上に述べたように、
光ファイバー方式は槽外方式と比較して伝達できる光エ
ネルギー量は大きいが、光拡散体の素材によっては、無
菌培養を行うときの運転管理が極めて煩雑である。本発
明は、従来装置のこれらの欠陥を改善し、無菌培養が省
エネルギー的で運転操作も著しく容易な新規な光合成微
生物の培養装置を提供することを課題とするものであ
る。[Problems to be Solved by the Invention] As described above,
The optical fiber method can transmit a large amount of light energy as compared with the method outside the tank, but depending on the material of the light diffuser, operation management during aseptic culture is extremely complicated. It is an object of the present invention to provide a novel photosynthetic microorganism culturing apparatus that improves these deficiencies of the conventional apparatus and that is capable of energy-saving aseptic culturing and remarkably easy operation.
【０００５】[0005]
【課題を解決するための手段】上記課題を解決するため
に、本発明では、光ファイバーにより伝送された光線を
光拡散体により培養槽内に放出して行う光合成微生物の
培養方法において、前記光拡散体が紫外線透過性を有
し、該光拡散体を介して槽内に随時紫外線を放出させて
滅菌することとしたものである。In order to solve the above-mentioned problems, the present invention provides a method for culturing a photosynthetic microorganism, wherein the light transmitted by an optical fiber is emitted into a culture tank by a light diffuser. The body is transparent to ultraviolet rays, and ultraviolet rays are occasionally emitted into the tank through the light diffuser to sterilize it.
【０００６】また、本発明では、光源と、光線を伝送す
る光ファイバーと、伝送された光線を培養槽内に放出す
る光拡散体とを有する光合成微生物の培養装置におい
て、前記光源として培養用光源と滅菌用紫外線源とを備
え、それらの光線のいずれかを光ファイバーを通して光
拡散体に導通させるための光路切換手段を有すると共
に、該光路切換手段、光ファイバー及び光拡散体をいず
れも紫外線透過性の素材により構成したものである。Further, according to the present invention, in a culturing apparatus for a photosynthetic microorganism comprising a light source, an optical fiber for transmitting a light beam, and a light diffuser for emitting the transmitted light beam into a culture tank, a light source for culturing is used as the light source. A sterilizing ultraviolet ray source, and an optical path switching means for conducting any one of the light rays to the light diffuser through the optical fiber, and the optical path switching means, the optical fiber and the light diffuser are all materials that are ultraviolet transparent. It is configured by.
【０００７】次に、本発明の一例を図１によって詳細に
説明する。光合成微生物の培養を行う場合は、太陽光及
び／又は人工光１は光ファイバー４を通じて培養槽５内
の培養液に挿入された側面より光を放出する光拡散体６
に導入され、槽内液に均等に拡散される。培養に必要な
ガス、例えば空気、二酸化炭素富化空気又は二酸化炭素
９は散気管１０から槽内液に拡散されるが、ガス供給方
法としてはこれに限定されるものではなく、効率的に必
要なガスが培養液に溶解されるものであれば、どのよう
な方法でもよく、培養槽外にガス溶解槽を設ける方法等
によっても良い。槽内液と培養菌体あるいは藻体を均一
に混合し、かつ槽内液に対する二酸化炭素、酸素などの
ガス成分の溶解効率を上げるために、培養槽底部に攪拌
装置１２を設けているが、液の混合方法としてはこれに
限定されるものではなく、槽外に設置した循環ポンプに
より培養液を循環させる方法等によっても良い。Next, an example of the present invention will be described in detail with reference to FIG. When culturing a photosynthetic microorganism, sunlight and / or artificial light 1 emits light from the side surface inserted into the culture solution in the culture tank 5 through the optical fiber 4 into a light diffuser 6
And is evenly dispersed in the liquid in the tank. The gas required for the culture, for example, air, carbon dioxide-enriched air or carbon dioxide 9 is diffused from the air diffuser 10 into the in-tank liquid, but the gas supply method is not limited to this and is required efficiently. Any method may be used as long as such a gas can be dissolved in the culture solution, and a method of providing a gas dissolution tank outside the culture tank may be used. A stirrer 12 is provided at the bottom of the culture tank in order to uniformly mix the liquid in the tank with the cultured cells or algae and to increase the efficiency of dissolving gas components such as carbon dioxide and oxygen in the liquid in the tank. The method of mixing the liquid is not limited to this, and a method of circulating the culture liquid by a circulation pump installed outside the tank may be used.
【０００８】また、これらの構成については、先に本発
明者らが特願平３−２０６１９９号に開示した装置を適
用するとより効果的な培養が行える。培養槽の滅菌を行
う場合は光路切り換え装置３を切り換えて紫外線ランプ
２の光が光拡散体６に導入される。光拡散体６は紫外線
を透過する素材であれば無機あるいは有機素材のどのよ
うな素材でもよい。例えば、光通信用に使用されている
石英ファイバーを加工したもの等が適用できる。滅菌は
培養槽内に培養液を満たした状態で行っても、引き抜い
た状態で行ってもよい。通常水中では紫外線の透過率が
著しく減少するが、本装置は光拡散体を用いて液体中に
効率良く光を伝送する構造を有しているため、培養液を
満たした状態でも優れた滅菌効果を発揮する。従って培
養前はもちろん培養終了時の藻体収穫にあたっても確実
な滅菌が行える。Further, with respect to these constitutions, more effective culture can be carried out by applying the device previously disclosed in Japanese Patent Application No. 3-206199 by the present inventors. When sterilizing the culture tank, the light path switching device 3 is switched to introduce the light from the ultraviolet lamp 2 into the light diffuser 6. The light diffuser 6 may be any material such as an inorganic material or an organic material as long as it is a material that transmits ultraviolet rays. For example, a processed quartz fiber used for optical communication can be applied. The sterilization may be performed with the culture solution filled in the culture tank or withdrawn. Normally, the transmittance of ultraviolet rays is significantly reduced in water, but since this device has a structure that efficiently transmits light in a liquid using a light diffuser, it has an excellent sterilization effect even when it is filled with a culture solution. Exert. Therefore, reliable sterilization can be performed not only before culturing but also when harvesting algal cells at the end of culturing.
【０００９】[0009]
【作用】本発明は紫外線を透過する素材でできた側面よ
り光を放出する光拡散体を用いているため、光合成微生
物の生育に必要な可視光と、滅菌に有効な紫外線のいず
れも効率良く培養槽内に拡散することができる。さらに
本発明は光路切り換え装置を有し、同一の光拡散体を培
養と滅菌両方に使用できるため可視光と紫外線の切り換
えが容易に行え、無菌的な培養に必要な装置滅菌が容易
且つ効率的に行える。Since the present invention uses a light diffuser that emits light from the side made of a material that transmits ultraviolet rays, both visible light necessary for growth of photosynthetic microorganisms and ultraviolet rays effective for sterilization are efficiently used. It can diffuse into the culture tank. Furthermore, since the present invention has an optical path switching device and the same light diffuser can be used for both culture and sterilization, switching between visible light and ultraviolet light can be easily performed, and device sterilization required for aseptic culture is easy and efficient. You can do it.
【００１０】[0010]
【実施例】以下、実施例により本発明を具体的に説明す
るが、本発明はこれに限定されるものではない。 実施例１ 図１に示すような総容量３リットル有効容積２リットル
の藻類培養装置を用いて、別に培養した大腸菌を１mlあ
たり１０００個になるように添加し、滅菌実験を行い、
引き続き藻類培養実験を実施した。実験には比較例とし
て従来例の図２〜４の組合せのものも行った。図２は光
拡散体と外部紫外線照射の組合せであり、図３は光拡散
体と薬洗の組合せ、図４は外部照射と薬洗の組合せであ
る。そして、図において、１５は薬液タンク、１６は薬
液注入ポンプであり、また１７は培養用蛍光燈であり、
他の符号は図１と同じ意味である。EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited thereto. Example 1 Using an algae culturing apparatus having a total volume of 3 liters and an effective volume of 2 liters as shown in FIG. 1, 1,000 Escherichia coli cultivated separately were added to 1 ml, and a sterilization experiment was conducted.
Subsequently, an algal culture experiment was carried out. As a comparative example, the combination of the conventional examples shown in FIGS. 2 shows a combination of a light diffuser and external UV irradiation, FIG. 3 shows a combination of a light diffuser and chemical washing, and FIG. 4 shows a combination of external irradiation and chemical washing. In the figure, 15 is a chemical solution tank, 16 is a chemical solution injection pump, 17 is a fluorescent lamp for culture,
Other symbols have the same meanings as in FIG.
【００１１】各装置の設置面積（槽本体）は下記表１に
示す。The installation area (tank body) of each device is shown in Table 1 below.
【表１】 [Table 1]
【００１２】滅菌試験の条件は次のとおりである。 供試細菌：大腸菌・イー．コリ（Ｅ．Coli) Ｋ−１２ 平均紫外線照射量：１０mW・s/cm2 薬洗時の次亜塩素酸ソーダ濃度（Ｃｌ2 として）：３pp
m ポンプ流量：３０リットル／hr 藻類培養条件は次のとおりである。 使用培地：ＭＢＭ培地 供試藻体株：クロレラ エリプソイデア（ Chlorella e
llipsoidea )ＩＡＭＣ−２７ 光条件：平均照度 ６，０００lx 培養温度：２５℃ 培養期間：１週間The conditions of the sterilization test are as follows. Bacteria under test: E. coli E. E. Coli K-12 Average ultraviolet irradiation dose: 10 mW · s / cm 2 Sodium hypochlorite concentration during chemical washing (as Cl 2 ): 3 pp
m Pump flow rate: 30 liters / hr The algal culture conditions are as follows. Medium used: MBM medium Test algal strain: Chlorella elipsoida
llipsoidea) IAMC-27 Light conditions: Average illuminance 6,000 lx Culture temperature: 25 ° C Culture period: 1 week
【００１３】実験の結果は、滅菌処理時間１分での藻体
収量と検出細菌数について表２に、実験に要した時間を
表３に、実験に必要なユーティリティ量を表４に示す。The results of the experiment are shown in Table 2 for the yield of algae and the number of bacteria detected in a sterilization time of 1 minute, in Table 3 for the time required for the experiment, and in Table 4 for the amount of utility required for the experiment.
【表２】 [Table 2]
【００１４】[0014]
【表３】 [Table 3]
【００１５】[0015]
【表４】 [Table 4]
【００１６】本発明の装置によれば、表３に示すように
洗浄・培地供給等の手間もかからず、表２に示すとおり
雑菌による汚染もなく、表４に示すように廉価にまた、
表１に示すとおり省スペースで、高効率に微細藻類クロ
レラが培養できた。これによって、本発明の光合成微生
物培養方法及び装置の総合的優秀性が理解できる。According to the apparatus of the present invention, as shown in Table 3, there is no need for washing and supplying of a medium, there is no contamination by various bacteria as shown in Table 2, and the cost is low as shown in Table 4.
As shown in Table 1, the microalga Chlorella was cultivated with high efficiency and in a small space. From this, the overall superiority of the photosynthetic microorganism culture method and apparatus of the present invention can be understood.
【００１７】[0017]
【発明の効果】本発明によれば、次の様な作用効果を奏
する。 （１）太陽光及び／又は人工光を光ファイバーを伝送媒
体として培養槽内に挿入された複数の光拡散体から槽内
液に拡散させているため、槽外からの光拡散方式に対し
て極めて効率的で培養槽に大量の光エネルギーを供給す
ることができる。 （２）光拡散体は紫外線を透過するため、光路切り換え
装置により紫外線ランプの光を光拡散体に導入し培養槽
内の効率的な滅菌が行える。 （３）光拡散体は培養液内に保護管なしで直接挿入でき
るため、光の利用効率が高まる。 （４）蒸気発生機が不要になり、また培養槽、配管とも
に耐熱・耐圧構造が不要になり、維持管理も容易にな
る。また薬液洗浄が不要になるため維持管理が容易にな
り、すすぎ用滅菌水等も不要になる。従って、スペース
の節減が図れる。 （５）培養した藻体即ち有機物を利用するに際し、容易
かつ確実な生細胞パージが行えるので産業上の安全性が
向上する。According to the present invention, the following operational effects are exhibited. (1) Since sunlight and / or artificial light is diffused into the in-tank liquid from a plurality of light diffusers inserted in the culture tank using an optical fiber as a transmission medium, it is extremely suitable for the light diffusion method from the outside of the tank. It is efficient and can supply a large amount of light energy to the culture tank. (2) Since the light diffuser transmits ultraviolet rays, the light from the ultraviolet lamp can be introduced into the light diffuser by the optical path switching device to efficiently sterilize the culture tank. (3) Since the light diffuser can be directly inserted into the culture solution without a protective tube, the light utilization efficiency is improved. (4) A steam generator is not required, and a heat-resistant and pressure-resistant structure is not required for both the culture tank and the piping, which makes maintenance easy. Further, since cleaning with a chemical solution is unnecessary, maintenance is facilitated, and sterilizing water for rinsing is also unnecessary. Therefore, space can be saved. (5) When utilizing cultured algal cells, that is, organic substances, live cell purging can be performed easily and surely, so that industrial safety is improved.
【図１】本発明の光合成微生物培養装置を示す概略構成
図である。FIG. 1 is a schematic configuration diagram showing a photosynthetic microorganism culture device of the present invention.
【図２】光拡散体と外部紫外線照射を組合せた従来装置
の概略図である。FIG. 2 is a schematic view of a conventional device in which a light diffuser and external ultraviolet irradiation are combined.
【図３】光拡散体と薬洗を組合せた従来装置の概略図で
ある。FIG. 3 is a schematic view of a conventional device in which a light diffuser and a chemical wash are combined.
【図４】外部照射と薬洗を組合せた従来装置の概略図で
ある。FIG. 4 is a schematic view of a conventional device that combines external irradiation and chemical washing.
【符号の説明】 １：太陽光及び／又は人工光源、２：紫外線ランプ、
３：光路切り換え装置、４：光ファイバー、５：培養
槽、６：光拡散体、７：培養液、８：余剰菌体又は藻
体、９：培養用ガス、１０：散気管、１１：排ガス、１
２：攪拌装置、１５：薬液タンク、１６：薬液注入ポン
プ、１７：培養用蛍光燈[Explanation of Codes] 1: Sunlight and / or artificial light source, 2: UV lamp,
3: optical path switching device, 4: optical fiber, 5: culture tank, 6: light diffuser, 7: culture solution, 8: surplus bacterial cells or algae, 9: culture gas, 10: air diffuser, 11: exhaust gas, 1
2: Stirrer, 15: Chemical solution tank, 16: Chemical solution injection pump, 17: Culture fluorescent lamp
───────────────────────────────────────────────────── フロントページの続き (72)発明者 梅田 到 神奈川県藤沢市本藤沢４丁目２番１号 株 式会社荏原総合研究所内 (72)発明者 滝沢 悦子 神奈川県藤沢市本藤沢４丁目２番１号 株 式会社荏原総合研究所内 (72)発明者 宮 晶子 神奈川県藤沢市本藤沢４丁目２番１号 株 式会社荏原総合研究所内 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Toru Umeda 4-2-1 Motofujisawa, Fujisawa-shi, Kanagawa Ebara Research Institute, Inc. (72) Inventor Etsuko Takizawa 4-2-1, Fujisawa, Kanagawa No. 1 Ebara Research Institute, Inc. (72) Inventor Akiko Miya, 4-2-1 Motofujisawa, Fujisawa City, Kanagawa Prefecture Ebara Research Institute, Inc.
Claims (2)
拡散体により培養槽内に放出して行う光合成微生物の培
養方法において、前記光拡散体が紫外線透過性を有し、
該光拡散体を介して槽内に随時紫外線を放出させて滅菌
することを特徴とする光合成微生物培養方法。1. A method of culturing a photosynthetic microorganism, which comprises radiating a light beam transmitted by an optical fiber into a culture tank by means of a light diffuser, wherein the light diffuser has ultraviolet transparency.
A method for culturing a photosynthetic microorganism, which comprises sterilizing by optionally emitting ultraviolet rays into the tank through the light diffuser.
と、伝送された光線を培養槽内に放出する光拡散体とを
有する光合成微生物の培養装置において、前記光源とし
て培養用光源と滅菌用紫外線源とを備え、それらの光線
のいずれかを光ファイバーを通して光拡散体に導通させ
るための光路切換手段を有すると共に、該光路切換手
段、光ファイバー及び光拡散体をいずれも紫外線透過性
の素材により構成したことを特徴とする光合成微生物培
養装置。2. A culturing apparatus for a photosynthetic microorganism, comprising a light source, an optical fiber for transmitting light rays, and a light diffuser for emitting the transmitted light rays into a culture tank, wherein the light source for culturing and the ultraviolet light source for sterilization are used. And an optical path switching means for conducting any one of the light rays to the light diffuser through the optical fiber, and the optical path switching means, the optical fiber and the light diffuser are all made of an ultraviolet-transparent material. A photosynthetic microorganism culture device characterized by the above.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4777992A JPH0710230B2 (en) | 1992-02-05 | 1992-02-05 | Photosynthetic microorganism culture method and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4777992A JPH0710230B2 (en) | 1992-02-05 | 1992-02-05 | Photosynthetic microorganism culture method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05219940A true JPH05219940A (en) | 1993-08-31 |
| JPH0710230B2 JPH0710230B2 (en) | 1995-02-08 |
Family
ID=12784863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4777992A Expired - Lifetime JPH0710230B2 (en) | 1992-02-05 | 1992-02-05 | Photosynthetic microorganism culture method and device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0710230B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011062397A3 (en) * | 2009-11-20 | 2011-11-03 | 한국기초과학지원연구원 | Microorganism culturing device using a plasma |
| WO2013063075A3 (en) * | 2011-10-24 | 2013-07-11 | Heliae Development Llc | Systems and methods for growing photosynthetic organisms |
-
1992
- 1992-02-05 JP JP4777992A patent/JPH0710230B2/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011062397A3 (en) * | 2009-11-20 | 2011-11-03 | 한국기초과학지원연구원 | Microorganism culturing device using a plasma |
| WO2013063075A3 (en) * | 2011-10-24 | 2013-07-11 | Heliae Development Llc | Systems and methods for growing photosynthetic organisms |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0710230B2 (en) | 1995-02-08 |
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