JPH0521699U - Incubator - Google Patents

Abstract

(57) [Summary] [Purpose] To prevent the air permeability of the vent holes from being impaired by depositing and depositing the culture on the air-permeable porous gas supply tube in the culture vessel. [Structure] A stirring member 3 for stirring the culture solution 5 is inserted into a container 1 containing the culture solution 5. In addition, an air-permeable porous gas supply pipe 6 for directly dissolving gas without generating bubbles in the culture solution is inserted into the container 1. On the way of the gas supply pipe 6, the gas supply pipe 6 is bent so as to form a plurality of upper and lower corrugations, and is arranged around the stirring member 6. The plurality of corrugated portions 6b, 6b of the gas supply pipe 6 are connected by the support member 7 to prevent the corrugated portion 6b from being deformed.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention is an apparatus for culturing a culture of cells, microorganisms, etc., which comprises an air-permeable porous gas supply pipe for directly dissolving the gas required for culturing in the culture solution without generating bubbles. It is about the improvement of what was done.

[0002]

[Prior Art]

 An example of a conventional culture device is shown in FIG. Reference numeral 11 is a culture container, and 12 is a lid thereof. A stirring member 13 is inserted into the container 11 through the lid 12 and has a stirring blade 13a at its lower end, which is rotated by a drive source (not shown). Reference numeral 14 is a dissolved oxygen amount detection device, and reference numeral 15 is a culture solution contained in the container 11. The gas supply pipe 16 is for dissolving a gas such as oxygen necessary for culture in the culture solution 15, and both ends thereof are connected to a gas supply device (not shown). The gas supply pipe 16 extends vertically downward through the lid 12 from the gas inlet side 16a, reaches the lower portion in the container 11, and is wound around the coil 16b and extends upward, and the gas outlet side 16c extends. It extends through 12. The coil-shaped portion 16b of the gas supply pipe 16 is made of a synthetic resin having a myriad of fine vent holes capable of directly dissolving the gas in the culture liquid 15 without generating bubbles, and the stirring member 13 Surrounding.

[0003]

[Problems to be solved by the device]

 In the above-mentioned conventional culture device, when the operation of the stirring member 13 is stopped for some reason and the stirring of the culture solution 15 is stopped, the cells and microorganisms in the culture solution 15 are deposited on the upper surface of the coiled portion 16b of the gas supply pipe 16. The cultures such as settled and adhered, blocking the ventilation holes of the gas supply pipe 16 and obstructing the ventilation. In addition, the culture grows in a state of adhering to the surface of the gas supply pipe 16 and is strongly bonded, so that there is a problem that it is difficult to separate the culture even if stirring is restarted. Therefore, it is an object of the present invention to provide a culture device in which the culture medium does not easily adhere to the gas supply pipe.

[0004]

[Means for Solving the Problems]

 In order to solve the above-mentioned problems, in the present invention, a container 1 containing a culture solution 5 for culturing a culture such as cells and microorganisms, and a culture solution 5 for agitating the culture solution 5 were inserted. A stirring member 3 and a gas-permeable pipe 6 of a gas permeable porous material for directly dissolving the gas in the culture medium 5 without generating bubbles are provided, and the gas supply pipe 6 is provided with a plurality of upper and lower waveforms. The culturing device was constructed by bending so as to form a fold and arranging it around the stirring member 3. Further, in order to prevent the deformation of the corrugated portion 6b, a large number of corrugated portions 6b of the gas supply pipe 6 were connected by a supporting member 7 to form a culture device.

[0005]

[Action]

 In the culture device of the present invention, the gas supply pipe 6 is bent up and down to form a large number of corrugations, so that most of the gas supply pipe 6 is inclined with respect to the horizontal. Therefore, even if the culture solution 5 stops stirring and the culture medium precipitates, it is difficult for the culture medium to adhere to the gas supply pipe 6, and the vent hole of the gas supply pipe 6 is not blocked. Further, when the corrugated portions 6b, 6b of the gas supply pipe 6 are connected by the support member 7, the relatively weak corrugated portion 6b is reinforced.

[0006]

【Example】

 An embodiment of the present invention will be described with reference to the drawings. In FIG. 1, reference numeral 1 is a culture container having a lid 2 on the top. A stirring member 3 penetrates through the lid 2 and is inserted into the container 1. The stirring member 3 has a stirring blade 3a at the lower end. The stirring member 3 is rotated by a drive source (not shown) so that the stirring blade 3a stirs the culture solution 5. Reference numeral 4 is a dissolved oxygen amount detection device, and 5 is a culture solution contained in the container 1. The gas supply pipe 6 is for dissolving a gas such as oxygen necessary for culture in the culture solution 5, and both ends thereof are connected to a gas supply device (not shown). The gas supply pipe 6 extends vertically downward through the lid 2 from the gas inlet side 6a, reaches the lower portion in the container 1, makes a round so as to surround the stirring member 3, and extends upward to extend the lid 2 To reach the gas delivery side 6c. A portion of the gas supply pipe 6 that surrounds the stirring member 3 forms a corrugated portion 6b that is bent vertically. The corrugated portion 6b is provided with innumerable fine vent holes capable of directly dissolving the gas in the culture liquid 5 without generating bubbles, and is made of, for example, a synthetic resin. The bent portions of the corrugated portion 6b are connected by a linear support member 7. The support member 7 is for preventing the corrugated portion 6b from being deformed or damaged.

In this culture apparatus, when the operation of the stirring member 3 is stopped for some reason, the culture medium in the culture solution 5 begins to settle, but since the upper surface of the corrugated portion 6b of the gas supply pipe 6 is inclined. It is difficult to adhere to this part, and the vent hole is not blocked. Further, the relatively weak corrugated portion 6b is reinforced by the support member 7 between the corrugated portions 6b, 6b, and durability is secured.

[0009]

[Effect of the device]

 As described above, according to the present invention, the container 1 for containing the culture solution 5 for culturing the culture such as cells and microorganisms, and the stirring member inserted into the culture solution 5 for stirring the culture solution 5 are provided. 3 and an air-permeable porous gas supply pipe 6 for directly dissolving a gas in the culture solution 5 without generating bubbles, and the gas supply pipe 6 is formed with a number of upper and lower corrugations. The culture device was constructed by bending and arranging it around the stirring member 3. Moreover, in order to prevent the deformation of the corrugated portion 6b of the gas supply pipe 6, a large number of corrugated portions 6b are connected by a supporting member 7 to form a culture device. Therefore, even if the culture solution 5 stops stirring for some reason and the culture medium precipitates, it does not adhere to the gas supply pipe 6 and the ventilation of the ventilation hole is not obstructed, so that the gas required for the culture is stored in the container. It can be supplied continuously. Further, the support member 7 between the corrugated portions 6b and 6b has an effect that the relatively weak corrugated portion 6b can be reinforced.

[Brief description of drawings]

FIG. 1 is a vertical cross-sectional view of a culture device of the present invention.

FIG. 2 is a vertical cross-sectional view of a conventional culture device.

[Explanation of symbols]

 1 Container 3 Stirring Member 5 Culture Liquid 6 Gas Supply Pipe 6b Corrugated Section 7 Supporting Member

Claims (2)

[Scope of utility model registration request] 1. A container for containing a culture solution for culturing a culture of cells, microorganisms, etc., a stirring member inserted in the culture solution for stirring the culture solution, and generation of bubbles in the culture solution. A gas supply pipe of a gas permeable porous material for directly dissolving the gas without the use of the gas supply pipe, the gas supply pipe is bent so as to form a plurality of upper and lower corrugations, and is provided around the stirring member. A culture device characterized by being arranged. 2. The culture device according to claim 1, wherein a plurality of corrugated portions of the gas supply pipe are connected by a supporting member to prevent deformation of the gas supply pipe.