CN208949315U - A kind of stem cell culture proliferation device - Google Patents

A kind of stem cell culture proliferation device Download PDF

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Publication number
CN208949315U
CN208949315U CN201821642408.9U CN201821642408U CN208949315U CN 208949315 U CN208949315 U CN 208949315U CN 201821642408 U CN201821642408 U CN 201821642408U CN 208949315 U CN208949315 U CN 208949315U
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China
Prior art keywords
box
reserve tank
liquid
stem cell
culture
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CN201821642408.9U
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Chinese (zh)
Inventor
周勇
王全立
陈海旭
耿培亮
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Xiamen Shenyan Regenerative Medicine Technology Co.,Ltd.
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Zhejiang Shenyan Precision Medical Technology Co Ltd
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Priority to CN201821642408.9U priority Critical patent/CN208949315U/en
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Abstract

The utility model discloses a kind of stem cells to cultivate proliferation device, including shell, liquid reserve tank, cultivate box, exhausted bath box, the enclosure interior is provided with the liquid reserve tank, the liquid reserve tank is internally provided with the first liquid level sensor, the liquid reserve tank side is provided with controller, the first solenoid valve is provided with below the liquid reserve tank, perfusion tube is provided with below first solenoid valve, the perfusion tube lower end is provided with the culture box, the culture box is internally provided with separation layer, temperature sensor is provided with above the separation layer, carbon dioxide sensor is provided with above the temperature sensor, heating plate is provided with below the culture box.Beneficial effect is: the utility model can not only carry out more changing jobs for culture solution automatically, easy to operate, easy to use, moreover it is possible to remind staff in time in culture solution deficiency and more waste liquid, ensure that stem cell cultivates value-added stability.

Description

A kind of stem cell culture proliferation device
Technical field
The utility model relates to field of biotechnology, cultivate proliferation device more particularly to a kind of stem cell.
Background technique
Stem cell is the initial cell of lactation biology, has multi-lineage potential and the of self-replication capacity, and stem cell can increase Value develops into the skin of lactation biology, internal organ etc., just has great medical meaning, biological meaning etc. to the research of stem cell.
Application No. is 201721041909.7 Chinese patents for comparison, disclose a kind of Culture of neural stem cells device, relate to And cell biology, the Culture of neural stem cells device include that can found culture vessel, container cover outward turning or are buckled in and can stand outside The bottle mouth position of culture vessel, culture vessel inner wall ecto-entad are disposed with culture medium layer and albumin coated layer, culture medium The pit that the bottom surface of layer is provided with;Through-hole is offered at the top of container cover, through-hole is operated bottle cap and passed through spiral shell by operation bottle cap sealing Line or buckle or shaft are connect with container cover.
Above-mentioned patent cannot replace culture solution automatically, and staff is needed to carry out the replacement of culture solution manually, cumbersome, It is inconvenient to use, it is therefore desirable to design a kind of stem cell culture proliferation device, the utility model can not only carry out culture solution automatically More change jobs, it is easy to operate, it is easy to use, moreover it is possible to remind staff in time in culture solution deficiency and more waste liquid, protect It has demonstrate,proved stem cell and has cultivated value-added stability.
Utility model content
In order to overcome the above problem, the purpose of the utility model is to provide a kind of stem cells to cultivate proliferation device, excellent Point is to carry out more changing jobs for culture solution automatically, easy to operate, easy to use, moreover it is possible to guarantee that stem cell cultivates value-added stabilization Property.
The utility model achieves the above object through the following technical schemes:
A kind of stem cell culture proliferation device, including shell, liquid reserve tank, culture box, exhausted bath box, the enclosure interior setting There is the liquid reserve tank, the liquid reserve tank is internally provided with the first liquid level sensor, the first liquid level sensor model LM31- 0000F-001PG, the liquid reserve tank side are provided with controller, the controller model KY02S, and the liquid reserve tank lower section is set It is equipped with the first solenoid valve, is provided with perfusion tube below first solenoid valve, the perfusion tube lower end is provided with the culture box, The culture box is internally provided with separation layer, is provided with temperature sensor, the temperature sensor model above the separation layer For TSEV01, the temperature sensor top is provided with carbon dioxide sensor, the carbon dioxide sensor model MH- Z14, the culture box lower section are provided with heating plate, and the perfusion tube side is provided with oxygen intake pipe, the oxygen intake pipe On be provided with second solenoid valve, the oxygen intake pipe is provided with exhaust pipe far from the perfusion tube side, and the exhaust pipe is remote The oxygen intake pipe side is provided with drain pipe, the drain pipe is provided with the waste liquid far from described culture box one end Case, the exhausted bath box are internally provided with the second liquid level sensor, the second liquid level sensor model LM31-0000F- 001PG, the shell front are provided with control panel, and touch screen is provided on the control panel, is set below the touch screen It is equipped with loudspeaker, the loudspeaker, the touch screen, the temperature sensor, the heating plate, first solenoid valve, institute State second solenoid valve, first liquid level sensor, second liquid level sensor and the carbon dioxide sensor with it is described Controller electrical connection.
So set, can be by the on-off of perfusion tube described in first solenoid valve control, that is, control to the training It supports and injects culture solution in box, and waste liquid can be entered in the exhausted bath box by the drain pipe in the culture box, realize training The automatic replacement of nutrient solution, it is simple, convenient.
In above structure, the controller controls the work of the heating plate according to the detection data of the temperature sensor Make, stablizes the temperature in the culture box in set temperature, the controller is according to the inspection of the carbon dioxide sensor Measured data controls the work of the second solenoid valve, that is, controls whether to convey oxygen into the culture box, makes the training The gas concentration lwevel in box to be supported to stablize in carbon dioxide setting concentration, when replacing culture solution, first solenoid valve is opened, Culture solution in the liquid reserve tank is entered in the culture box by the perfusion tube, while the controller carries out timeworker Make, waste liquid under the action of pressure difference in the culture box is entered in the exhausted bath box by the drain pipe, when described first When level value is lower than the feed liquor prompting level value set in the liquid reserve tank that liquid level sensor detects, the loudspeaker hair Liquid feeding prompting sound out reminds the timely liquid feeding of staff, when liquid in the exhausted bath box that second liquid level sensor detects When place value is higher than the drain prompting level value set, the loudspeaker issues drain prompting sound, reminds staff clear in time Manage the exhausted bath box.
As a kind of optimal technical scheme of the utility model, the controller and the shell connect by the way that screw is fixed It connects, the control panel is fixedly connected with the shell by screw, and the loudspeaker and the control panel are solid by screw Fixed connection.
So set, the enclosure interior can be fixed on the controller, can will be fixed with described in the loudspeaker Control panel is stably fixed on the shell.
As a kind of optimal technical scheme of the utility model, the culture box and the shell connect by the way that card slot is fixed It connects.
So set, ensure that the connective stability of the culture box and the shell.
As a kind of optimal technical scheme of the utility model, the oxygen intake pipe and the culture box are solid by screw thread Fixed connection, the exhaust pipe are threadably secured with the culture box and connect.
So set, ensure that the connective stability of the oxygen intake pipe and the exhaust pipe and the culture box.
As a kind of optimal technical scheme of the utility model, the culture box and the exhausted bath box pass through the drain pipe Connection.
So set, being entered in the exhausted bath box convenient for the waste liquid in the culture box by the drain pipe.
As a kind of optimal technical scheme of the utility model, the liquid reserve tank and first liquid level sensor pass through spiral shell Nail is fixedly connected.
So set, first liquid level sensor can be stably fixed in the liquid reserve tank, convenient for detecting the storage Liquid level variation in liquid case.
As a kind of optimal technical scheme of the utility model, the exhausted bath box and second liquid level sensor pass through spiral shell Nail is fixedly connected.
So set, second liquid level sensor can be stably fixed in the exhausted bath box, it is described useless convenient for detecting Liquid level variation in liquid case.
The utility model beneficial effect achieved is: the utility model can not only carry out the replacement work of culture solution automatically Make, it is easy to operate, it is easy to use, moreover it is possible to remind staff in time in culture solution deficiency and more waste liquid, ensure that dry thin Born of the same parents cultivate value-added stability.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only Some embodiments of the utility model, for those of ordinary skill in the art, in the premise of not making the creative labor property Under, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is a kind of schematic diagram of internal structure of stem cell culture proliferation device described in the utility model;
Fig. 2 is a kind of main view of stem cell culture proliferation device described in the utility model;
Fig. 3 is a kind of right view of stem cell culture proliferation device described in the utility model;
Fig. 4 is a kind of circuit structure flow diagram of stem cell culture proliferation device described in the utility model.
The reference numerals are as follows:
1, the second liquid level sensor;2, box is cultivated;3, perfusion tube;4, separation layer;5, temperature sensor;6, carbon dioxide passes Sensor;7, the first solenoid valve;8, the first liquid level sensor;9, liquid reserve tank;10, heating plate;11, controller;12, the second electromagnetism Valve;13, oxygen intake pipe;14, exhaust pipe;15, drain pipe;16, shell;17, exhausted bath box;18, loudspeaker;19, control panel; 20, touch screen.
Specific embodiment
In the description of the present invention, it should be understood that term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower", The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of describing the present invention and simplifying the description, rather than indicate Or imply that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot understand For limitations of the present invention.In addition, term " first ", " second " etc. are used for description purposes only, and should not be understood as indicating Or it implies relative importance or implicitly indicates the quantity of indicated technical characteristic." first ", " second " etc. are defined as a result, Feature can explicitly or implicitly include one or more of the features.It is in the description of the present invention, unless another It is described, the meaning of " plurality " is two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally Connection;It can be mechanical connection, be also possible to be electrically connected;Can be directly connected, can also indirectly connected through an intermediary, It can be the connection inside two elements.For the ordinary skill in the art, on being understood by concrete condition State the concrete meaning of term in the present invention.
The utility model is described in further detail with reference to the accompanying drawing.
First embodiment:
As Figure 1-Figure 4, a kind of stem cell cultivates proliferation device, including shell 16, liquid reserve tank 9, culture box 2, waste liquid Case 17, shell 16 are internally provided with liquid reserve tank 9, and liquid reserve tank 9 is internally provided with the first liquid level sensor 8, the first liquid level sensor 8 Model LM31-0000F-001PG, effect are the liquid levels for detecting culture solution in liquid reserve tank 9, and 9 side of liquid reserve tank is provided with Controller 11,11 model KY02S of controller, effect are to control the work of equipment, are provided with the first solenoid valve below liquid reserve tank 9 7, the effect of the first solenoid valve 7 is to control the on-off of perfusion tube 3, is provided with perfusion tube 3 below the first solenoid valve 7, under perfusion tube 3 End is provided with culture box 2, and culture box 2 is internally provided with separation layer 4, and the effect of separation layer 4 is that stem cell is avoided to be discharged, isolation 4 top of layer is provided with temperature sensor 5,5 model TSEV01 of temperature sensor, and effect is that the temperature in detection culture box 2 becomes Change, carbon dioxide sensor 6,6 model MH-Z14 of carbon dioxide sensor are provided with above temperature sensor 5, effect is inspection The gas concentration lwevel in culture box 2 is surveyed, cultivates and is provided with heating plate 10 below box 2,3 side of perfusion tube is provided with oxygen air inlet Pipe 13, second solenoid valve 12 is provided on oxygen intake pipe 13, and the effect of second solenoid valve 12 is control oxygen intake pipe 13 On-off, oxygen intake pipe 13 are provided with exhaust pipe 14 far from 3 side of perfusion tube, and exhaust pipe 14 is set far from 13 side of oxygen intake pipe It is equipped with drain pipe 15, the waste liquid that the effect of drain pipe 15 is easy in culture box 2 is discharged in exhausted bath box 17, and drain pipe 15 is separate Culture 2 one end of box is provided with exhausted bath box 17, and exhausted bath box 17 is internally provided with the second liquid level sensor 1,1 type of the second liquid level sensor Number be LM31-0000F-001PG, effect be detect exhausted bath box 17 in liquid level variation, 16 front of shell is provided with control panel 19, touch screen 20 is provided on control panel 19, and loudspeaker 18, loudspeaker 18, touch screen 20, temperature are provided with below touch screen 20 Spend sensor 5, heating plate 10, the first solenoid valve 7, second solenoid valve 12, the first liquid level sensor 8,1 and of the second liquid level sensor Carbon dioxide sensor 6 is electrically connected with controller 11.
Second embodiment:
Second embodiment is with the difference of first embodiment: controller 11 is fixedly connected with shell 16 by screw, control Panel 19 processed is fixedly connected with shell 16 by screw, and loudspeaker 18 is fixedly connected with control panel 19 by screw.
Specifically, controller 11 is fixedly connected with shell 16 by screw, ensure that the connection of controller 11 Yu shell 16 Stability, control panel 19 are fixedly connected with shell 16 by screw, ensure that the stable connection of control panel 19 Yu shell 16 Property, loudspeaker 18 are fixedly connected with control panel 19 by screw, ensure that the stable connection of control panel 19 Yu loudspeaker 18 Property.
3rd embodiment:
The difference of 3rd embodiment and first embodiment is: oxygen intake pipe 13 and culture 2 company of being threadably secured of box It connects, exhaust pipe 14 is threadably secured with culture box 2 and connect.
Specifically, oxygen intake pipe 13 is threadably secured with culture box 2 and connect, and ensure that oxygen intake pipe 13 and culture The connective stability of box 2, convenient for the installation and removal of oxygen intake pipe 13, exhaust pipe 14 and culture 2 company of being threadably secured of box It connects, ensure that exhaust pipe 14 and cultivates the connective stability of box 2, convenient for the installation and removal of exhaust pipe 14.
When the utility model is used, controller 11 controls the work of heating plate 10 according to the detection data of temperature sensor 5 Make, makes to cultivate the temperature in box 2 and stablize in set temperature, controller 11 is according to the detection data control of carbon dioxide sensor 6 The work of second solenoid valve 12 processed, that is, control whether to convey oxygen into culture box 2, make to cultivate the carbon dioxide in box 2 Concentration is stablized in carbon dioxide setting concentration, and when replacing culture solution, the first solenoid valve 7 is opened, and the culture solution in liquid reserve tank 9 is logical It crosses perfusion tube 3 to enter in culture box 2, while controller 11 carries out timework, the waste liquid in box 2 is cultivated under the action of pressure difference Entered in exhausted bath box 17 by drain pipe 15, when in the liquid reserve tank 9 that the first liquid level sensor 8 detects level value lower than setting Feed liquor remind level value when, loudspeaker 18 issue liquid feeding prompting sound, remind the timely liquid feeding of staff, when the second level sensing When level value is higher than the drain prompting level value set in the exhausted bath box 17 that device 1 detects, loudspeaker 18 issues drain prompt Sound reminds staff to clear up exhausted bath box 17 in time.
The basic principles and main features and advantage of the utility model have been shown and described above.The technical staff of the industry It should be appreciated that the present utility model is not limited to the above embodiments, the above embodiments and description only describe this The principle of utility model, on the premise of not departing from the spirit and scope of the utility model, the utility model also has various change And improvement, these various changes and improvements fall within the scope of the claimed invention.

Claims (7)

1. a kind of stem cell cultivates proliferation device, including shell, liquid reserve tank, culture box, exhausted bath box, it is characterised in that: the shell Body is internally provided with the liquid reserve tank, and the liquid reserve tank is internally provided with the first liquid level sensor, the liquid reserve tank side setting There is controller, the first solenoid valve is provided with below the liquid reserve tank, is provided with perfusion tube below first solenoid valve, it is described defeated Liquid pipe lower end is provided with the culture box, and the culture box is internally provided with separation layer, is provided with temperature above the separation layer Sensor, the temperature sensor top is provided with carbon dioxide sensor, is provided with heating plate below the culture box, described Perfusion tube side is provided with oxygen intake pipe, and second solenoid valve is provided on the oxygen intake pipe, and the oxygen intake pipe is remote The perfusion tube side is provided with exhaust pipe, the exhaust pipe is provided with drain pipe far from the oxygen intake pipe side, institute It states drain pipe and described culture box one end is provided with the exhausted bath box, the exhausted bath box is internally provided with the second level sensing Device, the shell front are provided with control panel, and touch screen is provided on the control panel, is provided with below the touch screen Loudspeaker, the loudspeaker, the touch screen, the temperature sensor, the heating plate, first solenoid valve, described Two solenoid valves, first liquid level sensor, second liquid level sensor and the carbon dioxide sensor and the control Device electrical connection.
2. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the controller and the shell Body is fixedly connected by screw, and the control panel is fixedly connected with the shell by screw, the loudspeaker and the control Panel processed is fixedly connected by screw.
3. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the culture box and the shell Body is fixedly connected by card slot.
4. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the oxygen intake pipe and institute It states culture box and is threadably secured connection, the exhaust pipe is threadably secured with the culture box and connect.
5. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the culture box gives up with described Liquid case is connected by the drain pipe.
6. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the liquid reserve tank and described the One liquid level sensor is fixedly connected by screw.
7. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the exhausted bath box and described the Two liquid level sensors are fixedly connected by screw.
CN201821642408.9U 2018-10-10 2018-10-10 A kind of stem cell culture proliferation device Active CN208949315U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821642408.9U CN208949315U (en) 2018-10-10 2018-10-10 A kind of stem cell culture proliferation device

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Application Number Priority Date Filing Date Title
CN201821642408.9U CN208949315U (en) 2018-10-10 2018-10-10 A kind of stem cell culture proliferation device

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114181828A (en) * 2021-12-03 2022-03-15 深圳市新一仑生物科技有限公司 Stem cell automatic amplification culture equipment based on modularization

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114181828A (en) * 2021-12-03 2022-03-15 深圳市新一仑生物科技有限公司 Stem cell automatic amplification culture equipment based on modularization

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Effective date of registration: 20210531

Address after: 11 / F, building B13, Xiamen biomedical industrial park, 2074 wengjiao West Road, Haicang District, Xiamen City, Fujian Province, 361000

Patentee after: Xiamen Shenyan Regenerative Medicine Technology Co.,Ltd.

Address before: No.555-2, juding square, Taizhou Development Zone, 317300, Zhejiang Province

Patentee before: ZHEJIANG SHENYAN PRECISION MEDICAL TECHNOLOGY Co.,Ltd.

TR01 Transfer of patent right