CN208949315U - A kind of stem cell culture proliferation device - Google Patents
A kind of stem cell culture proliferation device Download PDFInfo
- Publication number
- CN208949315U CN208949315U CN201821642408.9U CN201821642408U CN208949315U CN 208949315 U CN208949315 U CN 208949315U CN 201821642408 U CN201821642408 U CN 201821642408U CN 208949315 U CN208949315 U CN 208949315U
- Authority
- CN
- China
- Prior art keywords
- box
- reserve tank
- liquid
- stem cell
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 26
- 230000035755 proliferation Effects 0.000 title claims abstract description 19
- 238000004113 cell culture Methods 0.000 title description 8
- 239000007788 liquid Substances 0.000 claims abstract description 79
- CURLTUGMZLYLDI-UHFFFAOYSA-N carbon dioxide Chemical compound data:image/svg+xml;base64,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 data:image/svg+xml;base64,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 O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 15
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims abstract description 10
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 23
- 239000001301 oxygen Substances 0.000 claims description 23
- MYMOFIZGZYHOMD-UHFFFAOYSA-N oxygen Chemical compound data:image/svg+xml;base64,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 data:image/svg+xml;base64,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 O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 23
- 230000000694 effects Effects 0.000 abstract description 11
- 239000002699 waste material Substances 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 description 4
- 229910004682 ON-OFF Inorganic materials 0.000 description 3
- 210000000282 Nails Anatomy 0.000 description 2
- 210000001178 Neural Stem Cells Anatomy 0.000 description 2
- 210000004027 cells Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000001963 growth media Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000006651 lactation Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 102100001249 ALB Human genes 0.000 description 1
- 101710027066 ALB Proteins 0.000 description 1
- 210000003491 Skin Anatomy 0.000 description 1
- 229940050528 albumin Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organs Anatomy 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Abstract
The utility model discloses a kind of stem cells to cultivate proliferation device, including shell, liquid reserve tank, cultivate box, exhausted bath box, the enclosure interior is provided with the liquid reserve tank, the liquid reserve tank is internally provided with the first liquid level sensor, the liquid reserve tank side is provided with controller, the first solenoid valve is provided with below the liquid reserve tank, perfusion tube is provided with below first solenoid valve, the perfusion tube lower end is provided with the culture box, the culture box is internally provided with separation layer, temperature sensor is provided with above the separation layer, carbon dioxide sensor is provided with above the temperature sensor, heating plate is provided with below the culture box.Beneficial effect is: the utility model can not only carry out more changing jobs for culture solution automatically, easy to operate, easy to use, moreover it is possible to remind staff in time in culture solution deficiency and more waste liquid, ensure that stem cell cultivates value-added stability.
Description
Technical field
The utility model relates to field of biotechnology, cultivate proliferation device more particularly to a kind of stem cell.
Background technique
Stem cell is the initial cell of lactation biology, has multi-lineage potential and the of self-replication capacity, and stem cell can increase
Value develops into the skin of lactation biology, internal organ etc., just has great medical meaning, biological meaning etc. to the research of stem cell.
Application No. is 201721041909.7 Chinese patents for comparison, disclose a kind of Culture of neural stem cells device, relate to
And cell biology, the Culture of neural stem cells device include that can found culture vessel, container cover outward turning or are buckled in and can stand outside
The bottle mouth position of culture vessel, culture vessel inner wall ecto-entad are disposed with culture medium layer and albumin coated layer, culture medium
The pit that the bottom surface of layer is provided with；Through-hole is offered at the top of container cover, through-hole is operated bottle cap and passed through spiral shell by operation bottle cap sealing
Line or buckle or shaft are connect with container cover.
Above-mentioned patent cannot replace culture solution automatically, and staff is needed to carry out the replacement of culture solution manually, cumbersome,
It is inconvenient to use, it is therefore desirable to design a kind of stem cell culture proliferation device, the utility model can not only carry out culture solution automatically
More change jobs, it is easy to operate, it is easy to use, moreover it is possible to remind staff in time in culture solution deficiency and more waste liquid, protect
It has demonstrate,proved stem cell and has cultivated value-added stability.
Utility model content
In order to overcome the above problem, the purpose of the utility model is to provide a kind of stem cells to cultivate proliferation device, excellent
Point is to carry out more changing jobs for culture solution automatically, easy to operate, easy to use, moreover it is possible to guarantee that stem cell cultivates value-added stabilization
Property.
The utility model achieves the above object through the following technical schemes:
A kind of stem cell culture proliferation device, including shell, liquid reserve tank, culture box, exhausted bath box, the enclosure interior setting
There is the liquid reserve tank, the liquid reserve tank is internally provided with the first liquid level sensor, the first liquid level sensor model LM31-
0000F-001PG, the liquid reserve tank side are provided with controller, the controller model KY02S, and the liquid reserve tank lower section is set
It is equipped with the first solenoid valve, is provided with perfusion tube below first solenoid valve, the perfusion tube lower end is provided with the culture box,
The culture box is internally provided with separation layer, is provided with temperature sensor, the temperature sensor model above the separation layer
For TSEV01, the temperature sensor top is provided with carbon dioxide sensor, the carbon dioxide sensor model MH-
Z14, the culture box lower section are provided with heating plate, and the perfusion tube side is provided with oxygen intake pipe, the oxygen intake pipe
On be provided with second solenoid valve, the oxygen intake pipe is provided with exhaust pipe far from the perfusion tube side, and the exhaust pipe is remote
The oxygen intake pipe side is provided with drain pipe, the drain pipe is provided with the waste liquid far from described culture box one end
Case, the exhausted bath box are internally provided with the second liquid level sensor, the second liquid level sensor model LM31-0000F-
001PG, the shell front are provided with control panel, and touch screen is provided on the control panel, is set below the touch screen
It is equipped with loudspeaker, the loudspeaker, the touch screen, the temperature sensor, the heating plate, first solenoid valve, institute
State second solenoid valve, first liquid level sensor, second liquid level sensor and the carbon dioxide sensor with it is described
Controller electrical connection.
So set, can be by the on-off of perfusion tube described in first solenoid valve control, that is, control to the training
It supports and injects culture solution in box, and waste liquid can be entered in the exhausted bath box by the drain pipe in the culture box, realize training
The automatic replacement of nutrient solution, it is simple, convenient.
In above structure, the controller controls the work of the heating plate according to the detection data of the temperature sensor
Make, stablizes the temperature in the culture box in set temperature, the controller is according to the inspection of the carbon dioxide sensor
Measured data controls the work of the second solenoid valve, that is, controls whether to convey oxygen into the culture box, makes the training
The gas concentration lwevel in box to be supported to stablize in carbon dioxide setting concentration, when replacing culture solution, first solenoid valve is opened,
Culture solution in the liquid reserve tank is entered in the culture box by the perfusion tube, while the controller carries out timeworker
Make, waste liquid under the action of pressure difference in the culture box is entered in the exhausted bath box by the drain pipe, when described first
When level value is lower than the feed liquor prompting level value set in the liquid reserve tank that liquid level sensor detects, the loudspeaker hair
Liquid feeding prompting sound out reminds the timely liquid feeding of staff, when liquid in the exhausted bath box that second liquid level sensor detects
When place value is higher than the drain prompting level value set, the loudspeaker issues drain prompting sound, reminds staff clear in time
Manage the exhausted bath box.
As a kind of optimal technical scheme of the utility model, the controller and the shell connect by the way that screw is fixed
It connects, the control panel is fixedly connected with the shell by screw, and the loudspeaker and the control panel are solid by screw
Fixed connection.
So set, the enclosure interior can be fixed on the controller, can will be fixed with described in the loudspeaker
Control panel is stably fixed on the shell.
As a kind of optimal technical scheme of the utility model, the culture box and the shell connect by the way that card slot is fixed
It connects.
So set, ensure that the connective stability of the culture box and the shell.
As a kind of optimal technical scheme of the utility model, the oxygen intake pipe and the culture box are solid by screw thread
Fixed connection, the exhaust pipe are threadably secured with the culture box and connect.
So set, ensure that the connective stability of the oxygen intake pipe and the exhaust pipe and the culture box.
As a kind of optimal technical scheme of the utility model, the culture box and the exhausted bath box pass through the drain pipe
Connection.
So set, being entered in the exhausted bath box convenient for the waste liquid in the culture box by the drain pipe.
As a kind of optimal technical scheme of the utility model, the liquid reserve tank and first liquid level sensor pass through spiral shell
Nail is fixedly connected.
So set, first liquid level sensor can be stably fixed in the liquid reserve tank, convenient for detecting the storage
Liquid level variation in liquid case.
As a kind of optimal technical scheme of the utility model, the exhausted bath box and second liquid level sensor pass through spiral shell
Nail is fixedly connected.
So set, second liquid level sensor can be stably fixed in the exhausted bath box, it is described useless convenient for detecting
Liquid level variation in liquid case.
The utility model beneficial effect achieved is: the utility model can not only carry out the replacement work of culture solution automatically
Make, it is easy to operate, it is easy to use, moreover it is possible to remind staff in time in culture solution deficiency and more waste liquid, ensure that dry thin
Born of the same parents cultivate value-added stability.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
Some embodiments of the utility model, for those of ordinary skill in the art, in the premise of not making the creative labor property
Under, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is a kind of schematic diagram of internal structure of stem cell culture proliferation device described in the utility model；
Fig. 2 is a kind of main view of stem cell culture proliferation device described in the utility model；
Fig. 3 is a kind of right view of stem cell culture proliferation device described in the utility model；
Fig. 4 is a kind of circuit structure flow diagram of stem cell culture proliferation device described in the utility model.
The reference numerals are as follows:
1, the second liquid level sensor；2, box is cultivated；3, perfusion tube；4, separation layer；5, temperature sensor；6, carbon dioxide passes
Sensor；7, the first solenoid valve；8, the first liquid level sensor；9, liquid reserve tank；10, heating plate；11, controller；12, the second electromagnetism
Valve；13, oxygen intake pipe；14, exhaust pipe；15, drain pipe；16, shell；17, exhausted bath box；18, loudspeaker；19, control panel；
20, touch screen.
Specific embodiment
In the description of the present invention, it should be understood that term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of describing the present invention and simplifying the description, rather than indicate
Or imply that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot understand
For limitations of the present invention.In addition, term " first ", " second " etc. are used for description purposes only, and should not be understood as indicating
Or it implies relative importance or implicitly indicates the quantity of indicated technical characteristic." first ", " second " etc. are defined as a result,
Feature can explicitly or implicitly include one or more of the features.It is in the description of the present invention, unless another
It is described, the meaning of " plurality " is two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, on being understood by concrete condition
State the concrete meaning of term in the present invention.
The utility model is described in further detail with reference to the accompanying drawing.
First embodiment:
As Figure 1-Figure 4, a kind of stem cell cultivates proliferation device, including shell 16, liquid reserve tank 9, culture box 2, waste liquid
Case 17, shell 16 are internally provided with liquid reserve tank 9, and liquid reserve tank 9 is internally provided with the first liquid level sensor 8, the first liquid level sensor 8
Model LM31-0000F-001PG, effect are the liquid levels for detecting culture solution in liquid reserve tank 9, and 9 side of liquid reserve tank is provided with
Controller 11,11 model KY02S of controller, effect are to control the work of equipment, are provided with the first solenoid valve below liquid reserve tank 9
7, the effect of the first solenoid valve 7 is to control the on-off of perfusion tube 3, is provided with perfusion tube 3 below the first solenoid valve 7, under perfusion tube 3
End is provided with culture box 2, and culture box 2 is internally provided with separation layer 4, and the effect of separation layer 4 is that stem cell is avoided to be discharged, isolation
4 top of layer is provided with temperature sensor 5,5 model TSEV01 of temperature sensor, and effect is that the temperature in detection culture box 2 becomes
Change, carbon dioxide sensor 6,6 model MH-Z14 of carbon dioxide sensor are provided with above temperature sensor 5, effect is inspection
The gas concentration lwevel in culture box 2 is surveyed, cultivates and is provided with heating plate 10 below box 2,3 side of perfusion tube is provided with oxygen air inlet
Pipe 13, second solenoid valve 12 is provided on oxygen intake pipe 13, and the effect of second solenoid valve 12 is control oxygen intake pipe 13
On-off, oxygen intake pipe 13 are provided with exhaust pipe 14 far from 3 side of perfusion tube, and exhaust pipe 14 is set far from 13 side of oxygen intake pipe
It is equipped with drain pipe 15, the waste liquid that the effect of drain pipe 15 is easy in culture box 2 is discharged in exhausted bath box 17, and drain pipe 15 is separate
Culture 2 one end of box is provided with exhausted bath box 17, and exhausted bath box 17 is internally provided with the second liquid level sensor 1,1 type of the second liquid level sensor
Number be LM31-0000F-001PG, effect be detect exhausted bath box 17 in liquid level variation, 16 front of shell is provided with control panel
19, touch screen 20 is provided on control panel 19, and loudspeaker 18, loudspeaker 18, touch screen 20, temperature are provided with below touch screen 20
Spend sensor 5, heating plate 10, the first solenoid valve 7, second solenoid valve 12, the first liquid level sensor 8,1 and of the second liquid level sensor
Carbon dioxide sensor 6 is electrically connected with controller 11.
Second embodiment:
Second embodiment is with the difference of first embodiment: controller 11 is fixedly connected with shell 16 by screw, control
Panel 19 processed is fixedly connected with shell 16 by screw, and loudspeaker 18 is fixedly connected with control panel 19 by screw.
Specifically, controller 11 is fixedly connected with shell 16 by screw, ensure that the connection of controller 11 Yu shell 16
Stability, control panel 19 are fixedly connected with shell 16 by screw, ensure that the stable connection of control panel 19 Yu shell 16
Property, loudspeaker 18 are fixedly connected with control panel 19 by screw, ensure that the stable connection of control panel 19 Yu loudspeaker 18
Property.
3rd embodiment:
The difference of 3rd embodiment and first embodiment is: oxygen intake pipe 13 and culture 2 company of being threadably secured of box
It connects, exhaust pipe 14 is threadably secured with culture box 2 and connect.
Specifically, oxygen intake pipe 13 is threadably secured with culture box 2 and connect, and ensure that oxygen intake pipe 13 and culture
The connective stability of box 2, convenient for the installation and removal of oxygen intake pipe 13, exhaust pipe 14 and culture 2 company of being threadably secured of box
It connects, ensure that exhaust pipe 14 and cultivates the connective stability of box 2, convenient for the installation and removal of exhaust pipe 14.
When the utility model is used, controller 11 controls the work of heating plate 10 according to the detection data of temperature sensor 5
Make, makes to cultivate the temperature in box 2 and stablize in set temperature, controller 11 is according to the detection data control of carbon dioxide sensor 6
The work of second solenoid valve 12 processed, that is, control whether to convey oxygen into culture box 2, make to cultivate the carbon dioxide in box 2
Concentration is stablized in carbon dioxide setting concentration, and when replacing culture solution, the first solenoid valve 7 is opened, and the culture solution in liquid reserve tank 9 is logical
It crosses perfusion tube 3 to enter in culture box 2, while controller 11 carries out timework, the waste liquid in box 2 is cultivated under the action of pressure difference
Entered in exhausted bath box 17 by drain pipe 15, when in the liquid reserve tank 9 that the first liquid level sensor 8 detects level value lower than setting
Feed liquor remind level value when, loudspeaker 18 issue liquid feeding prompting sound, remind the timely liquid feeding of staff, when the second level sensing
When level value is higher than the drain prompting level value set in the exhausted bath box 17 that device 1 detects, loudspeaker 18 issues drain prompt
Sound reminds staff to clear up exhausted bath box 17 in time.
The basic principles and main features and advantage of the utility model have been shown and described above.The technical staff of the industry
It should be appreciated that the present utility model is not limited to the above embodiments, the above embodiments and description only describe this
The principle of utility model, on the premise of not departing from the spirit and scope of the utility model, the utility model also has various change
And improvement, these various changes and improvements fall within the scope of the claimed invention.
Claims (7)
1. a kind of stem cell cultivates proliferation device, including shell, liquid reserve tank, culture box, exhausted bath box, it is characterised in that: the shell
Body is internally provided with the liquid reserve tank, and the liquid reserve tank is internally provided with the first liquid level sensor, the liquid reserve tank side setting
There is controller, the first solenoid valve is provided with below the liquid reserve tank, is provided with perfusion tube below first solenoid valve, it is described defeated
Liquid pipe lower end is provided with the culture box, and the culture box is internally provided with separation layer, is provided with temperature above the separation layer
Sensor, the temperature sensor top is provided with carbon dioxide sensor, is provided with heating plate below the culture box, described
Perfusion tube side is provided with oxygen intake pipe, and second solenoid valve is provided on the oxygen intake pipe, and the oxygen intake pipe is remote
The perfusion tube side is provided with exhaust pipe, the exhaust pipe is provided with drain pipe far from the oxygen intake pipe side, institute
It states drain pipe and described culture box one end is provided with the exhausted bath box, the exhausted bath box is internally provided with the second level sensing
Device, the shell front are provided with control panel, and touch screen is provided on the control panel, is provided with below the touch screen
Loudspeaker, the loudspeaker, the touch screen, the temperature sensor, the heating plate, first solenoid valve, described
Two solenoid valves, first liquid level sensor, second liquid level sensor and the carbon dioxide sensor and the control
Device electrical connection.
2. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the controller and the shell
Body is fixedly connected by screw, and the control panel is fixedly connected with the shell by screw, the loudspeaker and the control
Panel processed is fixedly connected by screw.
3. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the culture box and the shell
Body is fixedly connected by card slot.
4. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the oxygen intake pipe and institute
It states culture box and is threadably secured connection, the exhaust pipe is threadably secured with the culture box and connect.
5. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the culture box gives up with described
Liquid case is connected by the drain pipe.
6. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the liquid reserve tank and described the
One liquid level sensor is fixedly connected by screw.
7. a kind of stem cell according to claim 1 cultivates proliferation device, it is characterised in that: the exhausted bath box and described the
Two liquid level sensors are fixedly connected by screw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821642408.9U CN208949315U (en) | 2018-10-10 | 2018-10-10 | A kind of stem cell culture proliferation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821642408.9U CN208949315U (en) | 2018-10-10 | 2018-10-10 | A kind of stem cell culture proliferation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208949315U true CN208949315U (en) | 2019-06-07 |
Family
ID=66741060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821642408.9U Active CN208949315U (en) | 2018-10-10 | 2018-10-10 | A kind of stem cell culture proliferation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208949315U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114181828A (en) * | 2021-12-03 | 2022-03-15 | 深圳市新一仑生物科技有限公司 | Stem cell automatic amplification culture equipment based on modularization |
-
2018
- 2018-10-10 CN CN201821642408.9U patent/CN208949315U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114181828A (en) * | 2021-12-03 | 2022-03-15 | 深圳市新一仑生物科技有限公司 | Stem cell automatic amplification culture equipment based on modularization |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208949315U (en) | A kind of stem cell culture proliferation device | |
| CN201962180U (en) | Folding marsh gas collector of drift | |
| CN101221064B (en) | Low liquid level detecting device | |
| CN106479889A (en) | A kind of cell culture blood circulation | |
| CN106497784B (en) | A kind of control pressurer system and its compress control method for realizing culture solution and waste liquid exchange | |
| CN206799638U (en) | A kind of molecular biosciences experiment culture dish | |
| CN207552465U (en) | Molecular hydrogen generator and combination water tank | |
| CN208721451U (en) | Environmental pollution analyte detection sampling bottle | |
| CN101501493A (en) | Apparatus for measuring variations of extra-cellular membrane potential with microelectrodes | |
| CN208250338U (en) | A kind of Bacteria Culture device assembly | |
| CN203404595U (en) | Stainless steel wet type gas holder provided with inner guide rod | |
| CN206835942U (en) | Fish-feeding device and fish culture apparatus | |
| CN206347979U (en) | Battery separator maximum diameter of hole detection means | |
| CN203049334U (en) | Electric iron with water level reminding function | |
| CN206472834U (en) | A kind of food fermentation device of intelligent temperature control | |
| CN209546360U (en) | A kind of storage device for agri-foodstuffs | |
| CN104031833A (en) | Clutch gravity type fermentation and extraction integrated tank and extraction method thereof | |
| CN204355563U (en) | A kind of nylon salt aqueous system storage device | |
| CN211227125U (en) | Methane tank with improved structure | |
| CN203537922U (en) | Edible mushroom liquid fermentation tank | |
| CN201974406U (en) | Determination of small biological respiration device | |
| CN201971825U (en) | Culture fluid storage tank | |
| CN207803085U (en) | A kind of Kiwifruit Tissue Culture seedling rooting special box | |
| CN205993418U (en) | A kind of aquarium water level control device | |
| CN208472112U (en) | A kind of cell container for being beneficial to observe and cultivate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210531 Address after: 11 / F, building B13, Xiamen biomedical industrial park, 2074 wengjiao West Road, Haicang District, Xiamen City, Fujian Province, 361000 Patentee after: Xiamen Shenyan Regenerative Medicine Technology Co.,Ltd. Address before: No.555-2, juding square, Taizhou Development Zone, 317300, Zhejiang Province Patentee before: ZHEJIANG SHENYAN PRECISION MEDICAL TECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right |