JPH05184667A - Blood component separation bag and separation process for blood component - Google Patents
Blood component separation bag and separation process for blood componentInfo
- Publication number
- JPH05184667A JPH05184667A JP4023433A JP2343392A JPH05184667A JP H05184667 A JPH05184667 A JP H05184667A JP 4023433 A JP4023433 A JP 4023433A JP 2343392 A JP2343392 A JP 2343392A JP H05184667 A JPH05184667 A JP H05184667A
- Authority
- JP
- Japan
- Prior art keywords
- bag
- separation bag
- blood
- layer
- separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000926 separation method Methods 0.000 title claims abstract description 98
- 239000012503 blood component Substances 0.000 title claims description 26
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 45
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 43
- 210000004369 blood Anatomy 0.000 claims abstract description 37
- 239000008280 blood Substances 0.000 claims abstract description 37
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 30
- 239000003761 preservation solution Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 11
- 238000004555 blood preservation Methods 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- 210000002381 plasma Anatomy 0.000 abstract description 13
- 230000003582 thrombocytopenic effect Effects 0.000 abstract 1
- 239000000306 component Substances 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 5
- 210000000601 blood cell Anatomy 0.000 description 4
- 239000003755 preservative agent Substances 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 102000014150 Interferons Human genes 0.000 description 2
- 108010050904 Interferons Proteins 0.000 description 2
- 229940079322 interferon Drugs 0.000 description 2
- 230000001012 protector Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
Landscapes
- Medical Preparation Storing Or Oral Administration Devices (AREA)
- External Artificial Organs (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、供血者により採血した
血液を、高純度で赤血球、血小板、血漿等の各成分に分
画する血液成分分離バッグ及びこれを用いた血液成分の
分離方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood component separation bag for fractionating blood collected by a donor into high purity components such as red blood cells, platelets and plasma, and a method for separating blood components using the same. ..
【０００２】[0002]
【従来の技術】図４は、現在使用されている血液成分分
離バッグ６１の概略図である。血液成分分離バッグ６１
は、採血用バッグ６２とこれに接続される複数の分離用
バッグ６３、６４、６５とから構成される。2. Description of the Related Art FIG. 4 is a schematic view of a blood component separation bag 61 currently in use. Blood component separation bag 61
Is composed of a blood collection bag 62 and a plurality of separation bags 63, 64, 65 connected thereto.
【０００３】使用に際しては、供血者より血液を採血針
６７、採血チュ−ブ６８を経て採血用バッグ６２内に採
血し、強遠心分離処理して乏血小板血漿層（以下「ＰＰ
Ｐ」）、バフィ−コ−ト層、赤血球層（以下「ＣＲ
Ｃ」）に分離し、次の操作手順で血液の各成分を分離採
取していた。ＰＰＰを連結チュ−ブ７３、７５、７８
を経て分離用バッグ６５内に分取する工程、バフィ−
コ−ト層を連結チュ−ブ７３、７９を経て分離用バッグ
６３内に移す工程、In use, blood is collected from a donor through a blood collecting needle 67 and a blood collecting tube 68 into a blood collecting bag 62 and subjected to a strong centrifugal separation process to obtain a platelet poor plasma layer (hereinafter referred to as "PP").
P "), buffy coat layer, red blood cell layer (hereinafter" CR "
C ”), and each component of blood was separately collected by the following operation procedure. PPP connecting tubes 73, 75, 78
Buffing step of separating into the separation bag 65 via
Transferring the coat layer through the connecting tubes 73, 79 into the separating bag 63,
【０００４】分離用バッグ６４中に封入された赤血球
保存液を連結チュ−ブ７７、７５、７３を経て採血用バ
ッグ６２内に残った赤血球に加える工程、分離用バッ
グ６３内のバフィ−コ−ト層を軽遠心分離処理し、血小
板層（上層）と白血球・赤血球（下層）を分離する工
程、前記血小板を連結チュ−ブ７９、７５、７７を経
て分離用バッグ６４内に分取する工程、以上の各工程に
より、採血用バッグ６２内には赤血球保存液加赤血球、
分離用バッグ６４内には血小板、分離用バッグ６５内に
はＰＰＰ、分離用バッグ６３内には白血球・赤血球が分
取される。分離用バッグ６３内の白血球・赤血球、微少
の血漿は、更に操作を加えてインタ−フェロン等の調製
に用いられていた。The step of adding the red blood cell preservation solution enclosed in the separation bag 64 to the red blood cells remaining in the blood collection bag 62 through the connecting tubes 77, 75, 73, and the buffy coat in the separation bag 63. And a step of separating the platelet layer (upper layer) from the white blood cells / red blood cells (lower layer) by a light centrifugation process, and a step of separating the platelets into the separation bag 64 via the connecting tubes 79, 75, 77. By the above steps, the red blood cell preservation solution-added red blood cells in the blood collection bag 62,
Platelets are collected in the separation bag 64, PPP is collected in the separation bag 65, and white blood cells and red blood cells are collected in the separation bag 63. The white blood cells / red blood cells and minute plasma in the separation bag 63 were further manipulated and used to prepare interferon and the like.
【０００５】[0005]
【発明が解決しようとする課題】強遠心後、ＰＰＰ、バ
フィ−コ−ト層は、分離用バッグ６５、６３へ移される
が、そのとき連結チュ−ブ７５、７８内には、ＰＰＰ、
連結チュ−ブ７３、７９内には、バフィ−コ−ト層（Ｃ
ＲＣの場合もある）が満たされた状態になっている。連
結チュ−ブ７９内のバフィ−コ−ト層は、分離用バッグ
６５より適量のＰＰＰで分離用バッグ６３に洗い込まれ
るが、そのとき、連結チュ−ブ７９内には、バフィ−コ
−ト層の白血球、赤血球、血小板等の血球成分が残る。
残った血球成分は、分離用バッグ６３のバフィ−コ−ト
層を軽遠心した後、血小板を分離用バッグ６４に移す
際、連結チュ−ブ７５または７７内の赤血球保存液と共
に移されることになり、分離用バッグ６４に調製される
血小板中には、不純物として赤血球、白血球等の血球成
分と赤血球保存液が含まれることになる。本発明は、血
小板中に血液成分及び赤血球保存液が混入するのを防ぐ
方法及び血液成分分離バッグを提供することを目的とす
る。After the strong centrifugation, the PPP and the buffy coat layer are transferred to the separation bags 65 and 63. At that time, in the connecting tubes 75 and 78, the PPP,
In the connecting tubes 73 and 79, a buffy coat layer (C
(It may be RC) is being satisfied. The buffy coat layer in the connecting tube 79 is washed into the separating bag 63 with an appropriate amount of PPP than in the separating bag 65. At that time, the buffy coat is put in the connecting tube 79. Blood cell components such as white blood cells, erythrocytes, and platelets in the gut layer remain.
The remaining blood cell components are transferred together with the erythrocyte preservation solution in the connecting tube 75 or 77 when the platelets are transferred to the separation bag 64 after lightly centrifuging the buffy coat layer of the separation bag 63. Therefore, the platelets prepared in the separation bag 64 will contain blood cell components such as red blood cells and white blood cells and the red blood cell preservation solution as impurities. An object of the present invention is to provide a method for preventing blood components and a red blood cell preservation solution from being mixed in platelets, and a blood component separation bag.
【０００６】[0006]
【課題を解決するための手段】本発明は、血液保存液の
封入された採血用バッグ２（３２）に、連結チュ−ブ１
３（３３）、１９（３９）、接続管１４（３４）を介し
て、バフィ−コ−トの分離用バッグ３（３３）が接続さ
れ、該分離用バッグ３（３３）に連結チュ−ブ２１（５
１）を介して白血球等の分離用バッグ６または血小板の
分離用バッグ３６が接続され、前記接続管１４（４４）
に、連結チュ−ブ１５（４５）、１７（４７）と接続管
１６（４６）を介して赤血球保存液の封入された分離用
バッグ４（３４）が接続されるとともに、連結チュ−ブ
１８（４８）を介して血漿の分離用バッグ５（３５）が
接続された血液成分分離バッグ１（３１）を提供するも
のである。According to the present invention, a connecting tube 1 is attached to a blood collecting bag 2 (32) in which a blood preservation solution is enclosed.
3 (33), 19 (39) and the connecting pipe 14 (34) are connected to the bag 3 (33) for separating the buffy coat, and the connecting tube 3 (33) is connected to the separating bag 3 (33). 21 (5
The bag 6 for separating white blood cells or the like or the bag 36 for separating platelets is connected via 1), and the connecting pipe 14 (44)
Is connected to the separation tubes 4 (34) containing the erythrocyte preservation solution via the connection tubes 15 (45) and 17 (47) and the connection tube 16 (46), and the connection tube 18 is connected. The blood component separation bag 1 (31) to which the plasma separation bag 5 (35) is connected via (48) is provided.
【０００７】また本発明は、次の各工程よりなる前記発
明記載の血液成分分離バッグ１（３１）を用いた血液成
分の分離方法を提供するものである。 全血を収容した採血用バッグ２（３２）を遠心分離処
理して、乏血小板血漿層（上層）、バフィ−コ−ト層
（界面）、赤血球層（下層）に分離する工程、 前記乏血小板血漿層を連結チュ−ブ１３（４３）、１
５（４５）、１８（４８）を経て、血漿の分離用バッグ
５（３５）に採取する工程、 前記バフィ−コ−ト層を連結チュ−ブ１３（４３）、
１９（４９）を介して分離用バッグ３（３３）に採取す
る工程、 前記分離用バッグ３（３３）内のバフィ−コ−ト層を
遠心分離処理して、濃縮血小板層（上層）と白血球・赤
血球層（下層）に分離する工程、 前記白血球・赤血球層を連結チュ−ブ２１（５１）を
経て分離用バッグ６に分離し、分離用バッグ３内に血小
板を分取する工程、The present invention also provides a method for separating blood components using the blood component separation bag 1 (31) described in the above invention, which comprises the following steps. A step of centrifuging a blood collection bag 2 (32) containing whole blood to separate it into a platelet poor plasma layer (upper layer), a buffy coat layer (interface) and a red blood cell layer (lower layer); Connect plasma layer to tube 13 (43), 1
5 (45), 18 (48), and collecting into the plasma separation bag 5 (35), the buffy coat layer is connected to the tube 13 (43),
19 (49) to collect in the separation bag 3 (33), centrifuge the buffy coat layer in the separation bag 3 (33) to concentrate platelet layer (upper layer) and white blood cells A step of separating into a red blood cell layer (lower layer), a step of separating the white blood cell / red blood cell layer into a separation bag 6 through a connecting tube 21 (51), and collecting platelets in the separation bag 3.
【０００８】また本発明は、前記発明記載の〜の工
程と、前記発明記載のの工程に代えて、´バフィ−
コ−トの分離用バッグ３３内に、白血球・赤血球層等を
残し、血小板層を連結チュ−ブ５１を介して分離用バッ
グ５６に採取する工程、よりなる血液成分の分離方法を
提供するものである。Further, the present invention is a method of replacing the steps (1) to (3) described above and the steps described above.
A method for separating a blood component, which comprises a step of leaving a white blood cell / red blood cell layer and the like in the coat separation bag 33 and collecting the platelet layer in the separation bag 56 through the connecting tube 51. Is.
【０００９】[0009]
【作用】血小板を分離用バッグ１に分離採取する際、そ
の間の連結チュ−ブ等には、他の血球成分や赤血球保存
液は存在していないので、高純度の血小板を採取するこ
とができる。When the platelets are separated and collected in the separation bag 1, there is no other blood cell component or erythrocyte preservation solution in the connecting tube or the like between them, so that high-purity platelets can be collected. ..
【００１０】[0010]
【実施例】図１は、本発明の血液成分分離バッグ１の概
略図である。血液成分分離バッグ１は、採血用バッグ２
とこれに連結チュ−ブ、接続管を介して接続される複数
の分離用バッグ３、４、５、６とから構成される。1 is a schematic view of a blood component separation bag 1 of the present invention. The blood component separation bag 1 is a blood collection bag 2
And a plurality of separating bags 3, 4, 5, 6 connected to the connecting tube and connecting pipes.
【００１１】採血用バッグ２の上端部には、先端に採血
針７を接続した採血チュ−ブ８とプロテクタ−１０によ
り被冠された輸血口９と連通ピ−ス１１を装填した接続
チュ−ブ１２が接続されている。また採血用バッグ２の
中には、血液保存液が封入されている。At the upper end of the blood collection bag 2, a blood collection tube 8 having a blood collection needle 7 connected to its tip, a blood transfusion port 9 capped by a protector 10 and a connection tube 11 having a communicating piece 11 loaded therein. Cable 12 is connected. A blood preservation solution is enclosed in the blood collection bag 2.
【００１２】前記採血用バッグ２には、連結チュ−ブ１
３、接続管１４、連結チュ−ブ１９を介して、バフィ−
コ−トの分離用バッグ３が接続されている。分離用バッ
グ３の下部は、漏斗状に形成され、その最下端部には、
連通ピ−ス２０を装填した排出チュ−ブ２４が接続され
ている。さらに分離用バッグ３には連結チュ−ブ２１を
介して白血球等の分離用バッグ６が接続されている。分
離用バッグ６は白血球等の不要な微少成分を分離採取す
るだけであるから、容量は小さくても良い。The blood collecting bag 2 includes a connecting tube 1
3, the connecting pipe 14, and the connecting tube 19
The coat separating bag 3 is connected. The lower part of the separation bag 3 is formed in a funnel shape, and the lowermost part thereof has
A discharge tube 24 loaded with the communication piece 20 is connected. Further, a separation bag 6 for separating white blood cells and the like is connected to the separation bag 3 via a connecting tube 21. Since the separation bag 6 only separates and collects unnecessary minute components such as white blood cells, it may have a small capacity.
【００１３】さらに、分離用バッグ３よりも下流に赤血
球保存液の封入された分離用バッグ４と血漿の分離用バ
ッグ５が接続されている。分離用バッグ４は、前記接続
管１４に連結チュ−ブ１５、接続管１６、連結チュ−ブ
１７、連通ピ−ス２３の装填された接続チュ−ブ２２を
介して接続されている。前記分離用バッグ５は、前記接
続管１６に連結チュ−ブ１８を介して接続されている。Further, a separation bag 4 in which a red blood cell preservation solution is sealed and a separation bag 5 for plasma are connected downstream of the separation bag 3. The separation bag 4 is connected to the connecting pipe 14 through a connecting tube 15, a connecting pipe 16, a connecting tube 17, and a connecting tube 22 in which a communicating piece 23 is loaded. The separating bag 5 is connected to the connecting pipe 16 via a connecting tube 18.
【００１４】次に、血液成分分離バッグ１の使用方法
（血液成分の分離方法）について説明する。供血者に採
血針７を穿刺して、血液を採血チュ−ブ８を経て採血用
バッグ２内に採血する。採血した血液を強遠心分離処理
して、ＰＰＰ（上層）バフィ−コ−ト層（白血球、血小
板を含む界面層）、ＣＲＣ（下層）に分離する。Next, a method of using the blood component separation bag 1 (a method of separating blood components) will be described. The donor is punctured with the blood collection needle 7, and blood is collected in the blood collection bag 2 through the blood collection tube 8. The collected blood is subjected to a strong centrifugation treatment to separate into a PPP (upper layer) buffy-coat layer (interface layer containing white blood cells and platelets) and CRC (lower layer).
【００１５】連通ピ−ス１２を切断して、上層のＰＰＰ
を接続チュ−ブ１２、連結チュ−ブ１３、１５、１８を
経て分離用バッグ５内に分取する。続いてバフィ−コ−
ト層を連結チュ−ブ１３、１９を経て分離用バッグ３内
に分取する。The communication piece 12 is cut to remove the upper layer PPP.
Is separated into the separating bag 5 through the connecting tube 12 and the connecting tubes 13, 15, and 18. Then Buffy
The sheet layer is separated into the separation bag 3 through the connecting tubes 13 and 19.
【００１６】連結チュ−ブ１９をシ−ルして分離用バッ
グ３、６を採血用バッグ２、分離用バッグ４、５から切
り離す。しかる後、分離用バッグ３内のバフィ−コ−ト
層を、軽遠心分離処理して血小板（上層）と白血球・赤
血球層（下層）に分離する。続いて連通ピ−ス２０を切
断して、白血球・赤血球層を排出チュ−ブ２４を経て分
離用バッグ６内に分離採取する。血小板（上層）と白血
球・赤血球（下層）の間の界面層は細径の排出チュ−ブ
内で長くなるので、分離が容易となる。最終的に、分離
用バッグ３内には、赤血球保存液が混入せずかつ赤血球
・白血球が完全に除かれた高純度の血小板を分取するこ
とができる。The connecting tube 19 is sealed to separate the separating bags 3 and 6 from the blood collecting bag 2 and the separating bags 4 and 5. Thereafter, the buffy coat layer in the separation bag 3 is subjected to a light centrifugation treatment to separate it into platelets (upper layer) and white blood cells / red blood cell layers (lower layer). Then, the communication piece 20 is cut, and the white blood cell / red blood cell layer is separated and collected in the separation bag 6 through the discharge tube 24. Since the interface layer between platelets (upper layer) and white blood cells / red blood cells (lower layer) becomes longer in the small-diameter draining tube, separation is facilitated. Finally, high-purity platelets in which the red blood cell preservative solution is not mixed and the red blood cells and white blood cells are completely removed can be collected in the separation bag 3.
【００１７】また、分離用バッグ３と分離用バッグ６の
間に設けられた排出チュ−ブ２４内には、連通ピ−ス２
０は必ずしも配置する必要はない。この場合、バフィ−
コ−トの軽遠心分離処理を行う際、図２に示すような分
離用バッグ３、６が固定可能な専用の固定部材２６を装
填した遠心分離用バケット２５を用いて、遠心処理と同
時に血小板と白血球・赤血球を分離する。遠心分離処理
後、分離用バッグ６内に血小板（上層）と白血球・赤血
球（下層）が分画され、血小板を連結チュ−ブ２１を経
て分離用バッグ３内に分取する。分離用バッグ３内に
は、赤血球保存液が混入せず赤血球・白血球が完全に除
かれた高純度の血小板を分取することができる。In the discharge tube 24 provided between the separation bag 3 and the separation bag 6, the communication piece 2 is placed.
0 does not necessarily have to be arranged. In this case,
At the time of performing the light centrifuge treatment of the coat, the platelets at the same time as the centrifuge treatment are carried out by using the centrifuge bucket 25 loaded with the exclusive fixing member 26 capable of fixing the separation bags 3 and 6 as shown in FIG. And white blood cells / red blood cells are separated. After the centrifugal separation treatment, platelets (upper layer) and white blood cells / red blood cells (lower layer) are fractionated in the separation bag 6, and the platelets are separated into the separation bag 3 via the connecting tube 21. High-purity platelets free of red blood cells and white blood cells can be collected in the separation bag 3 without being mixed with the red blood cell preservative solution.
【００１８】図３は、その他の血液成分分離バッグ３１
の概略図である。血液成分分離バッグ３１は、バフィ−
コ−トの分離用バッグ３３の上端に血小板の排出チュ−
ブ５４を設け連結チュ−ブ５１を介して分離用バッグ５
６を接続したものである。FIG. 3 shows another blood component separating bag 31.
FIG. The blood component separation bag 31 is a buffer
At the upper end of the coat separation bag 33, a platelet discharge tube is attached.
The separation bag 5 is provided with a tube 54 through the connecting tube 51.
6 is connected.
【００１９】使用に際しては、分離用バッグ３３内に採
取したバフィ−コ−トを軽遠心分離処理により血小板
（上層）と白血球・赤血球（下層）に分離し、血小板を
排出チュ−ブ５４、連結チュ−ブ５１を経て分離用バッ
グ３６内に分取する。分離用バッグ３６内には、赤血球
保存液が混入せずかつ白血球・赤血球が完全に除かれた
高純度の血小板を分取することができる。In use, the buffy coat collected in the separation bag 33 is separated into platelets (upper layer) and white blood cells / erythrocytes (lower layer) by a light centrifugation process, and the platelets are connected to the discharge tube 54. It is separated into the separation bag 36 through the tube 51. In the separation bag 36, high-purity platelets free of the red blood cell preservative solution and completely free of white blood cells and red blood cells can be collected.
【００２０】分離用バッグ５（３５）内に採取されたＰ
ＰＰは、連結チュ−ブ１８（３８）をシ−ル切断して個
別に分離でき、採血用バッグ２（３２）内に残ったＣＲ
Ｃは、分離用バッグ４（３４）の赤血球保存液を加え、
連結チュ−ブ１３（３３）をシ−ル切断して個別に分離
できる。他方分離用バッグ６または、バフィ−コ−トの
分離用バッグ３３内に残った白血球・赤血球は更に操作
を加えてインタ−フェロン等の調整に用いられる。P collected in the separation bag 5 (35)
PP can be separated by cutting the connecting tube 18 (38) by sealing, and the CR remaining in the blood collection bag 2 (32) can be separated.
C added the red blood cell preservation solution in the separation bag 4 (34),
The connecting tubes 13 (33) can be sealed and individually separated. On the other hand, the white blood cells / red blood cells remaining in the separation bag 6 or the separation bag 33 of the buffy coat are further manipulated and used for adjusting interferon and the like.
【００２１】[0021]
【発明の効果】以上説明したように本発明では、乏血小
板血漿、血小板、赤血球を効率良く分離採取することが
でき、特に血小板は高純度のものを分離採取することが
できる。INDUSTRIAL APPLICABILITY As described above, according to the present invention, platelet poor plasma, platelets and red blood cells can be efficiently separated and collected, and in particular, highly pure platelets can be separated and collected.
【図１】本発明の血液成分分離バッグの概略図FIG. 1 is a schematic view of a blood component separation bag of the present invention.
【図２】血小板の分離方法の一例を示す概略図FIG. 2 is a schematic diagram showing an example of a method for separating platelets.
【図３】血液成分分離バッグのその他の実施例を示す概
略図FIG. 3 is a schematic view showing another embodiment of the blood component separation bag.
【図４】従来の血液成分分離バッグの概略図FIG. 4 is a schematic view of a conventional blood component separation bag.
１、３１ 血液成分分離バッグ ２、３２ 採血用バッグ ３、３３ バフィ−コ−トの分離用バッグ ４、３４ 赤血球保存液の封入された分離用バッグ ５、３５ 血漿の分離用バッグ ６ 白血球等の分離用バッグ ７、３７ 採血針 ８、３８ 採血チュ−ブ ９、３９ 輸血口 １０、４０ プロテクタ− １１、４１ 接続チュ−ブ １２、４２ 連通ピ−ス １３、４３ 連結チュ−ブ １４、４４ 接続管 １５、４５ 連結チュ−ブ １６、４６ 接続管 １７、４７ 連結チュ−ブ １８、４８ 連結チュ−ブ １９、４９ 連結チュ−ブ ２０、５０ 連通ピ−ス ２１、５１ 連結チュ−ブ ２２、５２ 接続チュ−ブ ２３、５３ 連通ピ−ス ２４、５４ 排出チュ−ブ ２５ 遠心分離用バケット ３６ 血小板の分離用バッグ 1, 31 Blood Component Separation Bag 2, 32 Blood Collection Bag 3, 33 Buffy-Coat Separation Bag 4, 34 Separation Bag Enclosed with Red Blood Cell Preservation Solution 5, 35 Plasma Separation Bag 6 Leukocyte etc. Separation bag 7,37 Blood collection needle 8,38 Blood collection tube 9,39 Blood transfusion port 10,40 Protector 11,41 Connection tube 12,42 Communication piece 13,43 Connection tube 14,44 Connection Pipes 15, 45 Connection tubes 16, 46 Connection pipes 17, 47 Connection tubes 18, 48 Connection tubes 19, 49 Connection tubes 20, 50 Communication pieces 21, 51 Connection tubes 22, 52 connection tube 23, 53 communication piece 24, 54 discharge tube 25 centrifuge bucket 36 platelet separation bag
Claims (3)
（３２）に、連結チュ−ブ１３（３３）、１９（３
９）、接続管１４（３４）を介して、バフィ−コ−トの
分離用バッグ３（３３）が接続され、該分離用バッグ３
（３３）に連結チュ−ブ２１（５１）を介して白血球等
の分離用バッグ６または血小板の分離用バッグ３６が接
続され、前記接続管１４（４４）に、連結チュ−ブ１５
（４５）、１７（４７）と接続管１６（４６）を介して
赤血球保存液の封入された分離用バッグ４（３４）が接
続されるとともに、連結チュ−ブ１８（４８）を介して
血漿の分離用バッグ５（３５）が接続されたことを特徴
とする血液成分分離バッグ１（３１）。1. A blood collection bag 2 in which a blood preservation solution is enclosed.
(32), connecting tubes 13 (33), 19 (3
9), the buffy coat separating bag 3 (33) is connected via the connecting pipe 14 (34), and the separating bag 3
The separation bag 6 for separating white blood cells or the like or the separation bag 36 for platelets is connected to (33) through the connection tube 21 (51), and the connection tube 15 (15) is connected to the connection pipe 14 (44).
(45), 17 (47) and the connecting bag 16 (46) are connected to the separation bag 4 (34) in which the erythrocyte preservation solution is sealed, and plasma is connected through the connecting tube 18 (48). The blood component separation bag 1 (31), to which the separation bag 5 (35) is connected.
分分離バッグ１（３１）を用いた血液成分の分離方法。 全血を収容した採血用バッグ２（３２）を遠心分離処
理して、乏血小板血漿層（上層）、バフィ−コ−ト層
（界面）、赤血球層（下層）に分離する工程、 前記乏血小板血漿層を連結チュ−ブ１３（４３）、１
５（４５）、１８（４８）を経て、血漿の分離用バッグ
５（３５）に採取する工程、 前記バフィ−コ−ト層を連結チュ−ブ１３（４３）、
１９（４９）を介して分離用バッグ３（３３）に採取す
る工程、 前記分離用バッグ３（３３）内のバフィ−コ−ト層を
遠心分離処理して、濃縮血小板層（上層）と白血球・赤
血球層（下層）に分離する工程、 前記白血球・赤血球層を連結チュ−ブ２１（５１）を
経て分離用バッグ６に分離し、分離用バッグ３内に血小
板を分取する工程、2. A method for separating blood components using the blood component separation bag 1 (31) according to claim 1, which comprises the following steps. A step of centrifuging a blood collection bag 2 (32) containing whole blood to separate it into a platelet poor plasma layer (upper layer), a buffy coat layer (interface) and a red blood cell layer (lower layer); Connect plasma layer to tube 13 (43), 1
5 (45), 18 (48), and collecting into the plasma separation bag 5 (35), the buffy coat layer is connected to the tube 13 (43),
19 (49) to collect in the separation bag 3 (33), centrifuge the buffy coat layer in the separation bag 3 (33) to concentrate platelet layer (upper layer) and white blood cells A step of separating into a red blood cell layer (lower layer), a step of separating the white blood cell / red blood cell layer into a separation bag 6 through a connecting tube 21 (51), and collecting platelets in the separation bag 3.
記載のの工程に代えて´バフィ−コ−トの分離用バ
ッグ３３内に、白血球・赤血球層等を残し、血小板層を
連結チュ−ブ５１を介して分離用バッグ５６に採取する
工程、よりなることを特徴とする血液成分の分離方法。3. The steps (1) to (3) according to claim 2;
In place of the described step, a step of leaving the white blood cell / erythrocyte layer and the like in the separation bag 33 of the buffy coat and collecting the platelet layer in the separation bag 56 via the connecting tube 51, A method for separating blood components, which comprises:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4023433A JPH0741066B2 (en) | 1992-01-14 | 1992-01-14 | Blood component separation bag and blood component separation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4023433A JPH0741066B2 (en) | 1992-01-14 | 1992-01-14 | Blood component separation bag and blood component separation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05184667A true JPH05184667A (en) | 1993-07-27 |
| JPH0741066B2 JPH0741066B2 (en) | 1995-05-10 |
Family
ID=12110371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4023433A Expired - Fee Related JPH0741066B2 (en) | 1992-01-14 | 1992-01-14 | Blood component separation bag and blood component separation method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0741066B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020184020A1 (en) | 2019-03-14 | 2020-09-17 | テルモ株式会社 | Blood bag system |
| WO2020184019A1 (en) | 2019-03-14 | 2020-09-17 | テルモ株式会社 | Blood bag system |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102009022793A1 (en) * | 2009-05-27 | 2010-12-02 | Justus-Liebig-Universität Giessen | Apparatus and method for the production of blood products |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS633867A (en) * | 1986-06-24 | 1988-01-08 | 日本赤十字社 | Medical bag |
| JPS636876A (en) * | 1986-06-27 | 1988-01-12 | Hitachi Ltd | Semiconductor device |
| JP3094235U (en) * | 2001-12-17 | 2003-06-13 | 貞 植 尹 | Belt exchange type automatic telescopic device |
-
1992
- 1992-01-14 JP JP4023433A patent/JPH0741066B2/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS633867A (en) * | 1986-06-24 | 1988-01-08 | 日本赤十字社 | Medical bag |
| JPS636876A (en) * | 1986-06-27 | 1988-01-12 | Hitachi Ltd | Semiconductor device |
| JP3094235U (en) * | 2001-12-17 | 2003-06-13 | 貞 植 尹 | Belt exchange type automatic telescopic device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020184020A1 (en) | 2019-03-14 | 2020-09-17 | テルモ株式会社 | Blood bag system |
| WO2020184019A1 (en) | 2019-03-14 | 2020-09-17 | テルモ株式会社 | Blood bag system |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0741066B2 (en) | 1995-05-10 |
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