JPH08295629A - Separation of blood component - Google Patents

Abstract

PURPOSE: To obtain a blood preparation free from leukocyte from the blood in a blood bag. CONSTITUTION: The bag equipment for the separation of blood components is provided with a leukocyte-removing filter 5, a blood collection bag 3 connected to the upstream side of the leukocyte-removing filter 5 and at least two bags for the separation of blood components aseptically connected to the downstream side of the leukocyte-removing filter 5 through a tube. An air- containing bag 12 is aseptically connected through a tube to a blood bag 6 for holding the leukocyte-free blood to discharge the air from the blood bag 6.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood component separation method for removing leukocytes from blood in a blood bag.

[0002]

2. Description of the Related Art At present, a component transfusion method for transfusing only components required by a patient, that is, a blood product for transfusion is widely used. There are three types of blood products for transfusion: plasma products processed from whole blood, platelet products, and red blood cell products. Aseptic-controlled blood bag sets are used as containers for blood products for transfusion.

The blood bag set is composed of a blood collection needle, a blood collection bag filled with an anticoagulant, and an empty bag (separation bag for blood components) containing separated blood components. Each bag is aseptically connected by a tube. Has been done. In addition, a bag containing a blood preservation solution that can stably store blood for a long period of time is often incorporated in the blood bag set. Both blood bags are made of a flexible plastic material suitable for storing blood components.

In the processing of blood products for blood transfusion, the blood of healthy volunteers is collected in a blood collection bag, centrifugal load is applied using a centrifugal separator, and the blood components are separated into individual components by utilizing the difference in specific gravity. . As a result, plasma, platelets, white blood cells, and red blood cells are concentrated in this order from the upper layer of the blood collection bag. Next, plasma and platelets are transferred from the blood transfer tube above the blood collection bag to the blood component separation bag, and red blood cells are left in the blood collection bag for storage. At this time, leukocytes in the intermediate layer are mixed with each component, which causes side effects due to blood transfusion. Therefore, it is desired to remove leukocytes.

Known methods for removing leukocytes in blood include the washed red blood cell method, the thawed red blood cell method, and the white blood cell removal filter method. Among them, the filter method, which is a simple operation and has a high leukocyte removal rate, is widely used.

For example, a blood bag system incorporating a leukocyte removal filter is proposed in Japanese Patent Publication No. 6-59305. This is a system in which a leukocyte removal filter is arranged between a blood collection bag and a blood component separation bag to remove the white blood cells in the blood collection bag with a filter and then to separate it into plasma and red blood cells by centrifugation. However, since the bag for separating blood components is processed in a state in which air corresponding to the filter volume is mixed, it is not practical because factors that adversely affect the function of blood components and storage conditions occur.

[0007]

DISCLOSURE OF THE INVENTION The present invention is a method for separating blood components for removing leukocytes in blood, which prevents air from being mixed in a bag for separating blood components and which uses a leukocyte removal filter. The objective is to obtain blood products from whole blood in good yield.

[0008]

A first aspect of the present invention is to connect a blood collection bag upstream of a leukocyte removal filter and at least two blood component separation bags aseptically tubed downstream of the leukocyte removal filter. In the blood bag set connected by, the air containing bag is placed aseptically connected to the blood bag containing the blood after white blood cell removal, and the air in the blood bag containing the blood after white blood cell removal is arranged. A method for separating blood components is characterized in that

In a second aspect of the present invention, a blood collecting bag is connected to the upstream side of the leukocyte removal filter, and at least two blood component separation bags are aseptically connected to the downstream side of the leukocyte removal filter with a tube. In the bag set, the air bag is placed aseptically connected to the blood bag containing the blood after leukocyte removal by a tube, and the leukocyte removal filter downstream and the air bag are aseptically connected by a tube. A method of separating blood components is characterized in that the air is discharged from a blood bag containing the blood after removal of leukocytes and the residual blood at the downstream of the leukocyte removal filter is collected.

[0010]

The present invention is constructed as described above, that is, since the air bag is provided, the air in the blood component separating bag is removed. Further, by sending the air containing bag downstream of the leukocyte removal filter, a blood product can be obtained from whole blood with good yield.

[0011]

As described above, according to the present invention, the air bag is arranged in the blood bag set to which the leukocyte removal filter is connected, and the air in the bag containing the blood after leukocyte removal is reduced to reduce blood. After the preparation is obtained, adverse effects on blood components are reduced and blood remaining downstream of the leukocyte removal filter can be collected.

[0012]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below with reference to the drawings. FIG.
Is an example of the blood component separation method of the present invention.

Blood collected from the blood collection needle 1 is stored in a blood collection bag 3 containing an anticoagulant via a blood collection tube 2 and temporarily stored, and then leukocytes are removed from the blood transfer tube 4 through a leukocyte removal filter 5. The blood is stored in the accommodation bag 6. Next, the air existing in the bag 6 containing the blood from which white blood cells have been removed is expelled from the blood transfer tube 11 to the air containing bag 12. Blood transfer tube 4 '
And 11 are sealed and the leukocyte removal filter 5 and the air bag 12 are separated from the bag 6. Then, the blood in the storage bag 6 is separated into plasma and red blood cells by centrifugation. The plasma is moved to the blood separation bag 8 via the blood transfer tube 7. A preservative solution dedicated to the red blood cells left in the storage bag 6 is added to the red blood cells previously stored in the bag 10 through the blood transfer tube 9.

A clamp and a bent rod may be arranged and used in the middle of the blood transfer tube. FIG. 2 is another example of the method for separating blood components of the present invention, in which air existing in blood after leukocyte removal is expelled to the air containing bag 12 as described above, and then the blood transfer tube 11 is closed to transfer the blood. This is a method in which the blood remaining in the downstream of the leukocyte removal filter 5 is collected in the bag 6 by opening the tube 13.

[Brief description of drawings]

FIG. 1 is a system diagram of an example of a blood component separation method of the present invention.

FIG. 2 is a system diagram of another example of the blood component separation method of the present invention.

[Explanation of symbols]

 1 Blood Collection Needle 2 Blood Collection Tube 3 Blood Collection Bag 4 Blood Transfer Tube 5 Leukocyte Removal Filter 6 Blood Storage Bag 7 Blood Transfer Tube 8 Blood Separation Bag 9 Blood Transfer Tube 10 Blood Preservation Solution Bag 11 Blood Transfer Tube 12 Air Storage Bag

Claims (2)

[Claims] 1. A blood bag set comprising a blood collection bag connected upstream of a leukocyte removal filter and at least two blood component separation bags aseptically connected by a tube downstream of the leukocyte removal filter. Separation of blood components characterized by arranging an air containing bag aseptically connected to a blood bag containing the blood after removal by a tube and discharging the air in the blood bag containing the blood after removal of leukocytes Method. 2. A blood bag set comprising a blood collection bag connected upstream of a leukocyte removal filter and at least two blood component separation bags aseptically connected by a tube downstream of the leukocyte removal filter. The air bag is placed aseptically connected to the blood bag containing the blood after removal by a tube, and the leukocyte removal filter downstream and the air bag are aseptically connected by a tube to remove leukocytes. A method for separating blood components, characterized in that air in a blood bag containing the subsequent blood is discharged and residual blood downstream of the leukocyte removal filter is recovered.