JPH05170634A - Antiphotooxidizing skin medicine for external use - Google Patents

Abstract

PURPOSE:To obtain an antiphotooxidizing skin medicine for external use, containing an antiphotooxidizing substance prepared by culturing a sesame cell as an active ingredient, excellent in safety and usability and capable of exhibiting remarkable function to prevent the skin from deteriorating. CONSTITUTION:An antiphotooxidizing skin medicine for external use containing an eluate of a culture with a 40-60% alcohol obtained by inducing a callus of a sesame cell using a sesame sprouting aseptically germinated from a sesame seed as a material, providing a high-temperature cultured cell capable of stably carrying out subculture, culturing the cell in a culture medium and eluting the resultant culture with the alcohol. The alcohol eluate is contained in an amount of >=0.01wt.%, preferably 0.01-0.1wt.%, especially 0.5-1wt.%. This antiphotooxidizing skin medicine for external use is used as a milky lotion, a cosmetic base, a foundation, a hair tonic (alopecia and graying of hair are suppressed), etc.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin external preparation, and more particularly to an anti-photooxidative skin external preparation which prevents the effects of photooxidation on the skin.

[0002]

2. Description of the Related Art In recent years, acceleration of deterioration of the skin by light, especially ultraviolet rays has become a problem, and external preparations for skin have been developed in which various ultraviolet absorbers and the like are blended. However, these ultraviolet absorbers have a problem before the ultraviolet rays reach the skin, and it is extremely difficult to completely shield the ultraviolet rays. Therefore, a method for preventing the photodeterioration of the skin itself is being sought. Regarding the deterioration of the skin by light, recent studies have considered that the promotion of oxidation by light is one of the major causes. Therefore, it is possible to add an antioxidant to the external preparation for skin.

[0003]

Needless to say, various antioxidants have been conventionally blended in external preparations for skin such as cosmetics.
These are usually intended to prevent the oxidation of the components contained in the external preparation for skin itself. Then, as the inventors of the present invention proceeded with the study, it was found that even an antioxidant that is extremely effective in preventing the oxidation of the external preparation for skin itself cannot always obtain a sufficient effect regarding the photooxidation of the skin. It was revealed. That is, although synthetic antioxidants such as BHA and BHT are excellent in general antioxidative ability, the purpose of use and the amount used are markedly limited from the viewpoint of safety, and in particular, the effect of preventing skin deterioration is sufficient. Addition to the point of obtaining is not preferable. On the other hand, antioxidants such as α-tocopherol, which is less problematic in terms of safety, can be expected to have general antioxidative activity to some extent, but has extremely low antioxidative activity. The present invention has been made in view of the above problems of the prior art,
An object thereof is to provide an anti-photooxidation external preparation for skin which can directly prevent photooxidation of the skin.

[0004]

Means for Solving the Problems As a result of intensive studies by the present inventors in order to achieve the above-mentioned object, they found that sesame cell cultures have excellent antioxidative activity, and completed the present invention. Came to. That is, the anti-photooxidative external preparation for skin according to the present invention is prepared by culturing proliferating cells derived from plant bodies of sesame (Sesamun indicum L) in a medium,
-60% alcohol eluate.

The anti-oxidative skin external preparation according to the present invention preferably contains an alcohol eluate in an amount of 0.01% by weight or more. Further, when the external preparation according to the present invention is made non-oily, it is preferable that the alcohol eluate is contained in an amount of 0.01 to 0.1% by weight. Further, when the external preparation according to the present invention is oily, it is preferable that the alcohol eluate contains 0.5 to 1% by weight. Hereinafter, the configuration of the present invention will be described in more detail. The active ingredient in the present invention is sesame (S
Proliferative cells derived from the plant body of esamun indicum L) are cultured in a medium, and 40 to 60% alcohol eluate is obtained from the culture.

[0006] In order to breed sesame proliferating cells containing this useful component, sesame seedlings germinated aseptically from sesame seeds are used as a material to induce callus of sesame cells for stable subculture. Possible high temperature cultured cells were obtained. First, buds, roots, or seeds are used as sesame. Then, seedlings are prepared under aseptic conditions, and shoot, stem, leaf and / or root sections are cultured in a solid and / or liquid medium to induce callus cells. The resulting proliferative callus is subcultured to grow into a large callus. Then, this is allowed to stand and / or culture with stirring in solid and / or liquid culture to grow callus cells. As the medium,
Various media can be used. Then, an anti-photooxidative substance can be obtained by elution of 40-60% alcohol from the culture.

Further, when the external preparation according to the present invention is made non-oily, if the alcohol eluate is less than 0.01% by weight, the anti-photooxidation property cannot be sufficiently exhibited, and 0.1 Even if the amount is more than 10% by weight, the effect is not enhanced so much. Here, the non-oil external preparation for skin refers to an oily component of 5% by weight or less. On the other hand, when the external preparation according to the present invention is oily, if the alcohol eluate is less than 0.5% by weight, the photo-oxidation resistance cannot be sufficiently exhibited. Further, even if the amount is more than 1% by weight, the effect is not enhanced.

The external preparation for skin of the present invention contains, in addition to the above-mentioned active ingredients, various components generally used for external preparations for skin within the range that does not impair the effects of the present invention, that is, an aqueous component, a powder component, Oil, surfactant, moisturizer, thickener,
Preservatives, antioxidants, fragrances, dyes and the like can be added. Further, the dosage form of the external preparation for skin of the present invention is optional,
For example, it can be in the form of a solubilizing system such as lotion, an emulsifying system such as milky lotion or cream, or a formulation such as foundation or dispersion.

[0009]

EXAMPLES The constitution of the present invention will be described below in more detail based on examples. The present invention is not limited to the embodiments. In addition, as a general rule, the blending amount is expressed in% by weight.

Elution of Antiphotooxidizing Substance As described above, buds, roots or seeds are used as sesame. Then, seedlings are prepared under aseptic conditions, and shoot, stem, leaf and / or root sections are induced with a solid and / or liquid medium. Various media can be used as the media for the proliferating cells. As the carbon source, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, as well as polysaccharides such as oligos and starch can be used. As the nitrogen source, ammonium nitrate, nitrate nitrogen such as potassium nitrate, ammonium sulfate, other ammonium nitrogen such as ammonium tartrate, casamino acids, amino acids, peptone, corn steep liquor, yeast cells, yeast extract,
Malt extract etc. can be used. In addition, vitamins such as nicotinic acid, nicotinic acid amide, thiamine, folic acid, and biotin, nucleic acid-related substances such as inositol, adenylic acid, guanylic acid, cytidylic acid, thymidylic acid, and cyclic AMP, iron, manganese, zinc, boron, Iodine,
Minerals such as potassium, cobalt, magnesium, molybdenum, phosphorus and copper can also be used.

The basic medium is shown below. Ammonium sulfate 1650 mg Potassium nitrate 1900 Calcium chloride 440 Magnesium sulfate 370 Primary potassium phosphate 170 Boric acid 6.2 Manganese sulfate 22.3 Zinc sulfate 8.6 Iodine potassium 0.83 Sodium molybdate 0.25 Cobalt chloride 0.025 Copper sulfate 0.025 Ethylenediamine tetraacetate 37.3 Ferrous sulfate 27.8 Myo-inositol 100 glycine 2 pyridoxine hydrochloride 0.5 nicotinic acid 0.5 thiamine hydrochloride 0.1 sucrose 30 water 1000 ml pH 5.7 It is preferable to add auxin and cytokinin to the basic medium. As auxins, indole acetic acid, naphthalene acetic acid, 2,4 dichlorophenoxy acetic acid, etc. can be used. Used as appropriate. As the cytokinin, benzyladenine, kinetin, etc. can be used. These plant hormones and cytokinins can be used alone, but are preferably used in combination.

For the culture of proliferative callus, the above medium may be used, but in order to further improve the proliferative property, coconut milk, casein hydrolyzate, potato extract, corn steep liquor, yeast extract, malt extract It is preferable to add a natural organic nutrient source such as a liquid.

The culture can be performed at a culture temperature of 28 to 37 ° C, preferably 33 to 36 ° C. The pH of the culture solution is preferably weakly acidic (pH 5.6 to 6.0) for growth. In order to grow cells that can stably grow at high temperature from the sesame callus cells thus obtained, transplant a small mass of cells into a solid plate medium with gellan gum or agar, and grow the cells under the growth conditions described below. Incubate for 2 weeks. The culture temperature at this time is preferably maintained at 33 to 36 ° C.

Furthermore, by selecting a culture line with high proliferative ability from a large number of high-density cultured cell culture lines, extracting a large number of small clumps of cells from the cell population, and transplanting these into a new medium. , Make more culture lines. Subculture of such cell culture lines for 5-10
By repeating the operation once at a high temperature of 33 to 36 ° C., sesame cultured cells that can stably grow at a high temperature are grown. To elute anti-photooxidative substances from proliferating cells obtained by culturing,
The cells are disrupted by biological treatment using cellulase or lysozyme, chemical treatment, mechanical or ultrasonic treatment, or a combination thereof, and the cells are eluted with 40 to 60% methyl alcohol.

A specific manufacturing example will be described below. Preparation of aseptically grown sesame seedlings Sesame seeds are thoroughly washed with water and then immersed in a 75% ethanol aqueous solution for several seconds. Wash this with sterile water prepared separately,
Then, it is immersed in a 0.1% benzalkonium chloride (commercial fungicide) solution for 2 to 5 minutes to sterilize the microorganisms attached to the seeds. After thoroughly washing this seed with sterilized water,
With a disinfectant solution of 1% sodium hypochlorite (manufactured by Wako Pure Chemical Industries, Ltd.) (containing 0.1% of surfactant Tween 20),
The sesame seeds are completely sterilized by treating for 30 minutes.

On the other hand, a sterilized wide-mouthed container with a lid is prepared. A basal medium having the above composition (however, sucrose was not added, and 0.8 to 1.5% of agar was added as a solidifying agent and 0.2 to 0.3% of duran gum was added as a solidifying agent) was autoclaved separately. The product is poured into the wide-mouthed container and solidified to obtain a solid medium for seeding. Alternatively, sterile water and sterilized gauze may be aseptically placed in a wide-mouthed container to form a seeding bed. Sterilized sesame seeds are sown by aseptic operation in such a seeding medium or bed. 28-3
When kept in a thermostatic chamber at 0 ° C under the light of a fluorescent lamp, sterilized sesame seeds germinate without dying and sesame seedlings having a length of 3 to 5 cm can be prepared in about 10 days. This sesame seedling is completely sterile and is used for breeding proliferating cells.

Induction culture of sesame-derived proliferating cell mass In the basic medium having the above composition, naphthalene acetic acid (10 ⁻⁸ to 10 ⁻⁵ M) or 2,4 dichlorophenoxyacetic acid (10 ⁻⁸ to 10 ⁻⁵ ) was added as an auxin. M), benzyladenine (10 ⁻⁶ to 10 ⁻⁴ M) as cytokinin, or kinetin (10 ⁻⁶ to 10 ⁻⁴ M) in combination is added to prepare various media. Gellan gum 0.2% or agar 0.8% is added to this as a solidifying agent to adjust the pH to 5.7, and then it is dispensed into a Petri dish commonly used for culturing microorganisms and solidified. The sesame seedling fragments prepared aseptically as described above were transplanted into the wells at a temperature of 28-3.
When culturing is performed in a dark room at 0 ° C. in a constant temperature chamber or a constant temperature box, cells grow from the cut end of a sesame seedling cut piece after 2-3 weeks of culturing to form a callus as a mass. A large callus can be grown by subculturing this proliferative callus in a medium having the same composition.

The above-mentioned medium composition (Murashige-Skoog's medium) was used as the basal medium for artificially inducing callus, but any medium commonly used for plant cell culture can be used (plant cell culture). Manual, Kodansha 19
84). Propagation of callus cells Propagation of cultured cells Proliferative cell mass (callus) derived from sesame seedlings
A medium of the above composition, naphthalene acetic acid (1-5 × 10
^-5 M), benzyladenine (1-5 × 10 ^-5 M) and other auxins and cytokinins added, and further added with duran gum 0.2% or agar 0.8% as a solidifying agent to sterilize and solidify. The medium is used to grow stable growing cells.

Sesame cells proliferate well in the dark, but in the light, the proliferation is more active. That is, 3,000
~ 30,000 lux, preferably 8,000-15,
It is observed to grow well in the light of 000 lux. The temperature is preferably 30 to 37 ° C, preferably 33 to 36 ° C. In order to grow cells that can grow stably at a high temperature in sesame callus, a small cell mass is transplanted to a solid plate medium of duran gum or agar, and the cells are cultured under the above-mentioned growth conditions for 1 to 2 weeks. The culture temperature at this time is higher than the temperature used for induction culture of callus cells. That is, the culture temperature can be maintained at 33 to 36 [deg.] C. to concentrate the cells proliferating vigorously.

By selecting a proliferative and vigorous culture system from a large number of culture systems thus obtained, further extracting a large number of small clumps of cells from the cell population, and transplanting these into a new medium. , Again prepare multiple culture lines. By repeating the subculture of such a cell culture line 5 to 10 times, sesame cells capable of stable growth at a high temperature of 33 to 36 ° C are grown.

Cultivation of Proliferative Cells The culture medium of the above composition is used for the growth culture of sesame cells that can be stably subcultured, but a culture medium of the composition usually used for culturing plant cells can also be used. .. Naphthalene acetic acid (1-5 × 1
0 ^-5 M) and cytokinin to which benzyladenine (1-5 x 10 ^-5 M) was added are prepared. In the case of liquid culture, it can be used as it is for growth culture, but when it is cultured in a solid medium, it is sterilized and solidified by adding 0.2% of duran gum or 0.8% of agar. Irradiation with light is suitable for the growth of cells. Usually 3,000 to 30,000 lux, preferably 8,000
Irradiation is 0 to 15,000 lux. The temperature is 2
It grows at 8 to 37 ° C, preferably at 33 to 36 ° C. As the liquid culture, a shaking culture method or an aeration culture method used for usual culture of microorganisms can be applied, but it is preferable to operate under mild conditions as compared with the culture of microorganisms. Compared with the culture of microorganisms, the required amount of oxygen may be remarkably small, so it is preferable for agitation that the cells are not agitated at the bottom of the culture solution while slightly aerating the air.

In order to carry out the growth culture more effectively, it is preferable to maintain the pH of the culture solution at 5.6 to 5.8. In a normal proliferation culture, the culture is completed in 1 to 2 weeks, and thus the cells can be recovered from the culture medium by a conventional method such as a centrifugation method. In the case of solid culture,
The proliferated cell mass can be easily recovered. Thus, sesame cells that can be produced on an industrial scale can be cultivated, and a large amount of them can be grown and cultured. It should be noted that, compared with the amount of growth at a temperature of 25 to 28 ° C. which is usually used for culturing plant cells, the amount of growth is about twice or more at 35 to 36 ° C., and the growth rate also becomes faster. It can be effectively used in industrial plant cell culture.

Extraction and Chromatographic Elution The cells grown by the liquid culture method can be easily recovered by the gravity separation method or the centrifugal separation method. Ethyl alcohol is added to the obtained cells so that the final concentration is 80%, and the cells are extracted with a mechanical stirring homogenizer.
The remaining cells, which have been collected by centrifugation to recover the extract, are extracted again with 80% ethyl alcohol. The obtained extract is concentrated under reduced pressure and dried to give a candy-shaped, yellow-brown crude photo-oxidant substance extract.

This is, for example, Amberlite XAD-II
Is adsorbed to the resin by an adsorption chromatography method using, and eluted with 40 to 60% methyl alcohol to obtain a fraction having anti-photooxidation activity. Fractions eluted with 40% to 60% methyl alcohol were collected and concentrated under reduced pressure to obtain anti-photooxidative substances contained in sesame cultured cells (40 to 60% alcohol of the culture). Eluate).

Anti- Photooxidation Test First, prior to the description of specific examples of the external preparation for skin, the anti-oxidation test method thereof will be described. Since the purpose of the present invention is to prevent photooxidation of the skin, it is not appropriate to examine the antiphotooxidation property by a general lipid oxidation experiment or the like. Therefore, the inventors used the following method. First, a liposome suspension of 1.8 mM phosphatidylcholine / 0.1 M NaCl is prepared, and an appropriate amount of a test substance is added thereto. Then, photo-oxidation is performed at 25 ° C. for 10 hours using a Xe fade meter (long-wave ultraviolet UV-A). 1g of this Sun Pension is precisely weighed and 5ml
0.12% TBA (thiobarbituric acid) and 0.5
% TEA (triethylamine) / acetic acid solution,
React at 100 ° C. for 1 hour. Then, the suspension was measured for absorbance at 532 nm.

FIG. 1 shows a control (without addition of a test substance)
The TBA ratio is shown as the degree of deterioration. As is clear from the figure, 40% compared with α-tocopherol, which is the most common antioxidant added to external preparations for skin, etc.
It is understood that the methanol-eluted extract or the 60% methanol-eluted extract shows only about half the deterioration and has an extremely excellent antioxidative effect. Practical use test Phenomena due to skin deterioration include wrinkles, roughness, stains, and pigment abnormalities. Therefore, a skin external preparation containing the anti-photooxidant or α-tocopherol, which is a general antioxidant, is prepared, and the external preparation for 1
For a month, we tried to improve the skin deterioration phenomenon by actually using it on the panel where the skin deterioration phenomenon mentioned above was observed.

The evaluation was carried out as follows. +5: A remarkable improvement effect is seen. +3 ... Improving effect. +0 ... No particular change. -5 ... It got worse. The average of the respective evaluation points was obtained by 5 persons in each test section. A lotion having the following composition was used as a non-oil external preparation for skin.

[0028] Lotion basic composition alcoholic phase wt% 95% ethyl alcohol 25.0 lithospermi radix extract 0.5 Polyoxyethylene (60 mol) hardened castor oil ether 2.0 Perfume Appropriate amount aqueous phase glycerol phosphate disodium 0.05 Glycerin 5.0 Sodium hexametaphosphate Appropriate amount UV absorber Adequate amount Ion-exchanged water Residual <Production method> The aqueous phase and alcohol phase are prepared and solubilized.

The antiphotooxidizing substance or α-tocopherol according to the present invention was added in a predetermined amount to the alcohol phase so that the concentration shown in Table 1 was obtained. Then, Table 2 shows the results of the actual use test.

[Table 1]

[Table 2] As is clear from the above results, it was revealed that α-tocopherol was effective in improving the preservability of the cosmetic product itself, but had little effect on the anti-photooxidation property.

On the other hand, when an antiphotooxidizing substance is added,
If it is less than 0.01%, no clear skin deterioration preventing effect is observed, but if it is 0.01 to 0.1%, an excellent skin deterioration preventing effect is recognized. It should be noted that no significant difference was observed in the effect even if the content was more than 0.1%.

Next, an emulsion having the following composition was produced as an oily external preparation for skin. Stearic acid 2.5 Cetyl alcohol 1.5 Vaseline 7.0 Liquid paraffin 15.0 Polyoxyethylene (10 mol) monooleate 2.0 Polyethylene glycol 1500 3.0 Triethanolamine 1.0 Ascorbic acid 5.0 Glycero-3-phosphocholine 0.1 Perfume, appropriate amount, ion-exchanged water, residual <Production method> Ion-exchanged water with polyethylene glycol
0, triethanolamine, ascorbic acid, and glycerophosphocholine are added, and the mixture is heated and melted and kept at 70 ° C (aqueous phase). On the other hand, the other components are mixed, dissolved by heating and kept at 70 ° C. Add the oil phase to the water phase, emulsify evenly with a homomixer while performing pre-emulsification, and after stirring well, stir 30
Cool to ° C.

The anti-photooxidizing substance or α-tocopherol was added to the aqueous phase in a predetermined amount so that the concentration was as shown in Table 3. Then, Table 4 shows the results of the same actual use test as described above.

[Table 3]

[Table 4] From the above results, it was revealed that α-tocopherol had a small effect on the anti-photooxidation property similarly to the above-mentioned aqueous cosmetics. On the other hand, when an anti-photo-oxidative substance is added, it is 0.
If it is less than 5%, no clear improvement of the skin deterioration preventing effect is recognized, but if it is 0.5 to 1.0%, an excellent skin deterioration preventing effect is recognized. It should be noted that no significant difference was observed in the effect even if the content was more than 1.0%.

The optimum addition amount of the anti-photooxidative substance depends on whether or not the oil-based component is contained in a large amount, and thus the anti-photooxidant substance also has an antioxidant effect on the oil component as a cosmetic base. Therefore, it is considered that the cosmetic itself is consumed for antioxidant. Summarizing the above results, in the case of a non-oil skin external preparation containing only 5% or less of an oily component, the anti-photooxidizing substance according to the present invention is added in an amount of 0.01 to
It is preferable to contain 0.1%. Further, in the case of an oily external preparation for skin containing an oily component in an amount of more than 5%, it is understood that the optimum addition amount of the anti-photooxidation substance is 0.5 to 1.0%.

The anti-photooxidizing substance used in the present invention had good solubility and was completely dissolved in a non-oil or oily external preparation for skin. Next, specific examples of the anti-oxidative external preparation for skin according to the present invention will be described. It should be noted that the external preparations for skin according to each example also have an excellent effect of preventing skin deterioration.

Example 1 Nutritional emulsion oil Phase beeswax 1.0 Vaseline 2.0 Deodorized lanolin 1.5 Evening primrose oil 6.0 Cetyl isooctanoate 4.0 Polyoxyethylene (2 mol) oleyl ether 2.0 Ethyl Paraben 0.2 Butylparaben 0.1 Perfume 0.3 Water phase Carboxyvinyl polymer 0.2 Glycerol phosphate 0.1 Dipropylene glycol 2.0 L-Arginine 0.2 Purified water Residual 40% Methyl alcohol eluate 0 .7 <Production Method> The oil phase part and the water phase part are separately heated and dissolved by stirring. The oil phase is added to the water phase, and the mixture is emulsified and cooled to obtain a nutritional emulsion.

Example 2 Foundation Oil Phase Decamethylcyclopentasiloxane 21.6 Dimethylpolysiloxane (n = 5-20) 5.0 Trimethylsiloxysilicate 5.0 Squalane 5.0 Perfume 0.2 Polyoxyalkylene-modified Organopoly Siloxane 4.0 Dextrin fatty acid ester-treated powder 35.0 Aqueous phase ion-exchanged water Residual ethyl alcohol 10.0 Polyethylene glycol 3.0 Sodium L-glutamate 1.2 Sodium citrate 0.1 Sodium chondroitin sulfate 0.1 60% Methyl alcohol eluate 1.0 <Production method> The oil phase is stirred and mixed, and the aqueous phase is mixed and dissolved.
And both were mixed and the foundation was obtained.

Example 3 Toner Water Citric Acid 0.1 Zinc Sulfocarbonate 0.2 Glycerin 5.0 Polyoxyethylene (20 mol) Oleyl Alcohol Ether 1.0 Ethyl Alcohol 20.0 Purified Water Residual Fragrance 0.2 40 % Methyl alcohol eluate 0.05 <Production method> Citric acid, zinc sulfocarbonate, and glycerin are dissolved in purified water (aqueous phase). Polyoxyethylene oleyl alcohol ether in ethyl alcohol, fragrance <40%
Dissolve the methyl alcohol eluate (alcohol phase).
The alcohol phase is added to the aqueous phase to solubilize it, and the mixture is filtered.

Example 4 Hair Tonic Alcohol Phase 95% Ethyl Alcohol 10.0 Polyoxyethylene hydrogenated castor oil 2.0 Propylene glycol 4.0 Oleyl alcohol 0.1 L-menthol 0.5 40% Methyl alcohol eluate. 1 Water phase Ion-exchanged water Residual UV absorber Appropriate amount Glycerin 5.0 <Production method> After preparing the water phase and the alcohol phase respectively, solubilize them. According to the hair tonic according to the present embodiment,
Hair loss and graying due to scalp deterioration are suppressed.

[0039]

Industrial Applicability As described above, the anti-photooxidative external preparation for skin according to the present invention uses an anti-photooxidative substance obtained by culturing sesame cells as an active ingredient, and therefore has safety and usability. Excellent and has a remarkable effect of preventing skin deterioration.

[Brief description of drawings]

FIG. 1 is an explanatory view of the anti-photooxidative property of the active ingredient used in the external preparation for skin according to the present invention.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification number Office reference number FI technical display location (C12N 5/04 C12R 1:91) 7804-4B (72) Inventor Kiyotaka Kojima Ginza, Chuo-ku, Tokyo 7-5-5 Shiseido Co., Ltd. (72) Inventor Seio Mimura 453-7 Miyashita, Fuji City, Shizuoka Prefecture (72) Inventor Keiichi Takebayashi 2-18-5 Kasuga, Tsukuba City, Ibaraki Prefecture (72) Inventor Takahara Yoshimasa 5-29-8 Yatsu, Narashino City, Chiba Prefecture (72) Inventor Toshihiko Osawa 7-9-8 Oshizawadai, Kasugai City, Aichi Prefecture

Claims (4)

[Claims] 1. Proliferating cells derived from an adult plant of sesame (Sesamun indicum L) are cultured in a medium and 4
An anti-photooxidative external preparation for skin comprising 0 to 60% alcohol eluate. 2. The alcohol eluate according to claim 1,
An anti-photooxidative external preparation for skin, characterized by containing at least 01% by weight. 3. The alcohol eluate according to claim 1,
A non-oily anti-photooxidative external preparation for skin, characterized by containing 0.1 to 0.1% by weight. 4. The alcohol eluate according to claim 1
An oil-based anti-photooxidative external preparation for skin, characterized by containing 5 to 1% by weight.