JP3325619B2 - Production method of useful substance by tissue culture of Akane - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for efficiently producing useful substances in edible and medicinal madder.

[0002]

2. Description of the Related Art Akane has been used for a long time in both the east and west of the ocean. The method of use is to dig out the root part from October to November and dry it, and it is used for antitussive, expectorant, blood purification, menstruation, hemostasis and the like. On the other hand, it is widely used as a dye.

[0003] The genus Rubiaceae includes Rubia Akane (Rubian akane) and Rubia Cinctorum (Rubia tinctorum).
There are two main types. Akane from Honshu to Kyushu, Korea,
It is a perennial plant found in Taiwan, China, the Himalayas, Afghanistan, southern Europe along the Mediterranean coast, and western Asia, found in hills, forest margins, and mountains.

[0004] The active ingredients (useful substances) contained in madder include alizarin, purpurin, rubertophosphoric acid, rubian, rubiazine, xanthopurpurin, pseudopurpurin and the like. However, since they are not cultivated, they have no choice but to look for native ones, and the quality is not constant.

[0005]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a method for obtaining useful substances in madder more efficiently.

[0006]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, by culturing tissue, it has been possible to obtain an active ingredient of Akane having a high growth rate and constant quality. The present inventors have found that the intended purpose can be achieved and completed the present invention.

That is, the present invention is as follows.

[0008] (1) By the cultivation of madder tissue culture, alizarin, purpurin, rubertophosphate,
Rubian, rubiazine, xanthopurpurin, a method for producing useful substances such as pseudopurpurin, without adding plant hormones, seeds of rape seeds are cultured in a solid medium to induce callus, A method for producing a useful substance by tissue culture of madder, which comprises subculturing the callus in a solid medium without adding a plant hormone to redifferentiate the adventitious root and producing the adventitious substance in the adventitious root. .

(2) Alizarin, purpurin, rubertophosphate contained in madder,
Rubian, rubiazine, xanthopurpurin, a method for producing useful substances such as pseudopurpurin, without adding plant hormones, seeds of rape seeds are cultured in a solid medium to induce callus, The callus is cultured without adding a plant hormone, and is subcultured in a solid medium to regenerate and grow the seedlings. Production method of useful substances.

[0010] As the madder, seeds can be used. This is sterilized by a conventional method. In the present invention, the obtained adventitious roots and seedlings have almost no change in the content and the like of the produced pigment, but when arranged in descending order of the growth rate, the adventitious roots and seedlings are in the order.

[0011] The solid medium used for this purpose is not particularly limited, but may be Murashige a Skoog.
nd Skog's medium, White's medium, Gamborg et al.'s medium, Nitsch's medium, Heller's medium, Schenk Hildebrand's medium
Solid media such as d Hildebrandt's medium, Nitsch Nitsch's medium, Knop's medium, and Linsmeier and Skoog's medium can be used.

In the present invention, as shown in the embodiments described below,
Without adding cytokinin and auxin, which are known as plant growth regulators (plant hormones), useful substances can be produced by tissue culture of madder.

When saccharose is added, 2-4% by weight, pH 5.5-6.0, and the gelling agent may be agar or gellan gum.

[0014] If necessary, other medium-forming agents can also be added. In addition, the culture is performed at a temperature of 20 to 25 ° C.

[0015]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

(Example 1) (1) Rubia tinctor
um) seeds in 10% benzalkonium chloride 3-4
The sample was immersed in 70% ethanol for 1 to 2 seconds and 5% sodium hypochlorite for 1 to 5 minutes. After that, it was performed aseptically. (2) The seeds were inoculated on the following solid medium. The medium was adjusted to Murashige and Skoog's medium to 3% saccharose, 0.9% agar, and pH 5.8. (3) This was irradiated with light at 23 ° C. for 8 hours per day for 4 weeks and cultured. (4) Calli were taken out of the culture, subcultured to a medium of the same composition every month, and cultured for 4 months in a dark place.
The adventitious roots were redifferentiated, and the adventitious roots produced useful substances.

(Example 2) The same operation was performed for (1) to (3) of Example 1. From the culture, remove the callus and place it in a medium of the same composition.
The cells were subcultured every six months, cultured for 6 months under light irradiation, the plants were regenerated, and useful substances were produced in the roots.

[0018]

According to the present invention, it is possible to increase the growth rate of madder, and to efficiently produce madder of a certain quality such as alizarin without using plant hormones. And a method for producing a useful substance by tissue culture.

────────────────────────────────────────────────── ─── Continued on the front page (58) Fields investigated (Int. Cl. ⁷ , DB name) C12P 19/00-19/64 C12N 5/04 A01H 4/00 BIOSIS / MEDLINE / WPID S (STN) JICST file (JOIS)

Claims (2)

(57) [Claims] 1. A method for producing a useful substance such as alizarin, purpurin, rubertophosphate, rubian, rubiazine, xanthopurpurin, pseudopurpurin, etc., contained in madder by tissue culture of madder. Is cultured in a solid medium without adding a plant hormone to induce callus, and further, the callus is subcultured in a solid medium without the addition of a plant hormone to redifferentiate adventitious roots. A method for producing a useful substance by tissue culture of madder, which comprises producing a useful substance of madder. 2. A method for producing useful substances such as alizarin, purpurin, rubertophosphate, rubian, rubiazine, xanthopurpurine, pseudopurpurin, etc., contained in madder by tissue culture of madder. Is cultured in a solid medium without the addition of plant hormones to induce callus, and further, the callus is subcultured in a solid medium without the addition of plant hormones to regenerate young plants and grow. A method for producing a useful substance by cultivating a tissue of a madder, comprising causing the root to produce a useful substance of a madder after the letting.