JPH0513198Y2 - - Google Patents
Info
- Publication number
- JPH0513198Y2 JPH0513198Y2 JP5174787U JP5174787U JPH0513198Y2 JP H0513198 Y2 JPH0513198 Y2 JP H0513198Y2 JP 5174787 U JP5174787 U JP 5174787U JP 5174787 U JP5174787 U JP 5174787U JP H0513198 Y2 JPH0513198 Y2 JP H0513198Y2
- Authority
- JP
- Japan
- Prior art keywords
- cylindrical body
- lid
- valve
- sample
- presser
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 claims description 17
- 210000004027 cell Anatomy 0.000 description 4
- 230000006378 damage Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Description
【考案の詳細な説明】 イ 産業上の利用分野 本考案は細胞破壊装置に関するものである。[Detailed explanation of the idea] B Industrial application field The present invention relates to a cell destruction device.
ロ 従来の技術
従来に於ても単細胞組織、葉緑体、バクテリア
等の破壊装置は提案されているが、従来の装置は
油圧シリンダーにより油圧ピストンを操作し、試
料筒内の押圧子を押圧する構造のものが提案され
ている。又、モーター駆動方式にて押圧子を押圧
するものもある。B. Conventional technology Devices for destroying single-cell tissues, chloroplasts, bacteria, etc. have been proposed in the past, but the conventional devices operate a hydraulic piston using a hydraulic cylinder to press a presser inside a sample cylinder. structure has been proposed. In addition, there is also one that presses the presser using a motor drive method.
ハ 解決すべき問題点
然るに、上記の如く何れの装置も駆動部と直結
して試料筒の収納部を設ける必要がある構造であ
るため、装置が大型化し設置場所を広く必要とし
且つ装置は極めて高額なものとなつている。C. Problems to be solved However, as mentioned above, each device has a structure that requires a storage section for the sample tube to be directly connected to the drive unit, so the device becomes large and requires a large installation space. It has become expensive.
ニ 問題点を解決するための手段
然して細胞破壊した試料を分析するのに使用す
る液体クロマトグラフに於ては高圧ポンプにて送
液しておりその圧力を利用することにより駆動源
を直接内臓させなくとも細胞破壊装置が出来る点
に着目したもので、本考案は駆動源を別途取り出
す方式にすることにより装置を駆動源と分離して
構成し小型化し、軽量化し極めて廉価に提供でき
ると共に簡単に液体クロマトグラフの圧力液供給
部を着脱でき取扱も簡易なるように為さんとした
もので、筒状体にて試料収納部を形成させ該筒状
体上下に蓋体を設けると共に一方の蓋体に加圧液
の出入孔を出口孔にはバルブを設けて形成させ筒
状体内には筒状体に内接して押圧子を摺動自在に
設ける一方他の蓋体は着脱自在に為し且つバルブ
を備えた試料排出口を設けたことを特徴とする。D. Means to solve the problem However, liquid chromatographs used to analyze cell-disrupted samples use high-pressure pumps to pump liquid, and by utilizing the pressure, the driving source can be built directly into the liquid chromatograph. Focusing on the fact that at least a cell destruction device can be created, the present invention has a method in which the drive source is taken out separately, thereby making the device separate from the drive source, making it compact, lightweight, extremely inexpensive, and easy to provide. The pressure liquid supply section of the liquid chromatograph was designed to be detachable and easy to handle.The sample storage section is formed from a cylindrical body, and lids are provided above and below the cylindrical body. The outlet hole is provided with a valve to form an inlet/outlet hole for the pressurized liquid, and a presser is provided inside the cylindrical body so as to be slidable therein, while the other lid body is made detachable. It is characterized by having a sample outlet equipped with a valve.
ホ 実施例
本考案の一実施例を図により説明すると１は筒
状体で内部は細胞等の試料室２を形成させてあ
る。３は筒状体１の一方端に設けた蓋体で筒状体
に嵌合させてあり、袋ナツト４により筒状体に締
着されている。しかし、該蓋体３は他のネジ等に
より締着させることも又、筒状体２と一体的に形
成することも出来る。E. Embodiment An embodiment of the present invention will be described with reference to the drawings. Reference numeral 1 is a cylindrical body in which a sample chamber 2 for cells, etc. is formed. Reference numeral 3 denotes a lid provided at one end of the cylindrical body 1, which is fitted onto the cylindrical body, and is fastened to the cylindrical body with a cap nut 4. However, the lid 3 can be fastened with other screws or the like, or can be formed integrally with the cylindrical body 2.
５は蓋体３に形成させた加圧液の流入孔で、加
圧管の螺入部６を形成させ連通させてある。７は
加圧液の出口孔で排出管の所望箇処にバルブ９を
設けておく。１０はＯリングで蓋体３の周囲に設
け筒状体１に接せしめてある。１１は押圧子で筒
状体１内に収納し、Ｏリング１２，１３により筒
状体に摺動可能にしてある。１４は必要に応じて
設ける引出棒で一端は押圧子１１に固定し蓋体３
に設けた通孔１５を介して外側に突出させてあ
る。１６は筒状体１の他方端に設けた蓋体でＯリ
ング１７を介して筒状体１に嵌合させ袋ナツト１
８を以つて筒状体に着脱自在に為してある。１９
は蓋体１６に穿設した試料排出口で、排出管の螺
入部２０を形成連通し、排出管にはバルブ２１を
設けて流出量を調整するようにしてある。 Reference numeral 5 denotes an inflow hole for pressurized liquid formed in the lid 3, and a threaded portion 6 of a pressurizing tube is formed to communicate with the inflow hole. Reference numeral 7 indicates an outlet hole for the pressurized liquid, and a valve 9 is provided at a desired location on the discharge pipe. 10 is an O-ring provided around the lid 3 and in contact with the cylindrical body 1. Reference numeral 11 denotes a presser which is housed within the cylindrical body 1 and is made slidable onto the cylindrical body by means of O-rings 12 and 13. Reference numeral 14 denotes a pull-out rod provided as necessary, one end of which is fixed to the presser 11 and is attached to the lid body 3.
It is made to protrude outside through a through hole 15 provided in. Reference numeral 16 denotes a lid provided on the other end of the cylindrical body 1, which is fitted onto the cylindrical body 1 via an O-ring 17 and then screwed into the cap nut 1.
8 so that it can be attached to and detached from the cylindrical body. 19
A sample discharge port is formed in the lid body 16 and communicates with a threaded portion 20 of a discharge pipe.The discharge pipe is provided with a valve 21 to adjust the outflow amount.
次いでその作動について説明すれば蓋体１６を
袋ナツト１８の螺脱により外し、試料室２に試料
を収納し後逆操作にて蓋体１６を筒状体１に固定
する。 Next, to explain its operation, the lid 16 is removed by unscrewing the cap nut 18, a sample is stored in the sample chamber 2, and the lid 16 is fixed to the cylindrical body 1 by the reverse operation.
液体クロマトグラフの液送ポンプに連結した加
圧管を蓋体３の螺入部６に螺通固定し、排出管を
螺入部８に螺通固定する。バルブ９は閉じておき
加圧液を送入させる。 A pressurizing tube connected to a liquid feed pump of a liquid chromatograph is screwed and fixed to the screw portion 6 of the cover 3, and a discharge tube is screwed and fixed to the screw portion 8. The valve 9 is kept closed to feed the pressurized liquid.
通常この圧力は20〜450Kg／cm2を使用するが600
Kg／cm2を使用することも可能である。この加圧液
の流入により、押圧子１１は試料室２内を移動せ
しめられ、試料室２内の試料は加圧され排出管１
９を介し調整したバルブ２１より流出する。その
細胞は高圧でバルブ２１を通過することにより外
皮が破壊される。押圧子１１が前進し蓋体１６に
当ることにより全ての試料の処理が終わる。加圧
ポンプを止めた後ネジ１８をゆるめ蓋体１６を筒
状体１よりはずし、バルブ９を開放にし引出棒１
４を引き押圧子１１を蓋体３に引きよせる。その
後、筒状体１の内部を洗浄し再び試料を入れ再使
用する。 Normally this pressure is 20 to 450Kg/ cm2 , but 600Kg/cm2 is used.
It is also possible to use Kg/ cm2 . Due to the inflow of this pressurized liquid, the pusher 11 is moved within the sample chamber 2, and the sample within the sample chamber 2 is pressurized and the discharge pipe 1
It flows out from the regulated valve 21 via the valve 9. The cells are passed through valve 21 under high pressure, thereby destroying their outer skin. When the presser 11 moves forward and hits the lid 16, processing of all samples is completed. After stopping the pressure pump, loosen the screw 18, remove the lid body 16 from the cylindrical body 1, open the valve 9, and remove the pull-out rod 1.
4 to pull the pusher 11 towards the lid body 3. Thereafter, the inside of the cylindrical body 1 is cleaned, and a sample is again put into the cylindrical body 1 for reuse.
引出棒１４を設けない場合は、試料収納時等に
旧位置に復帰させる。 If the pull-out rod 14 is not provided, it is returned to the old position when storing the sample.
不使用時には加圧液の加圧管は螺脱して外して
おくことも出来る。 When not in use, the pressure tube for pressurized fluid can be unscrewed and removed.
ヘ 考案の作用、効果
上記の如き本考案によれば筒状体にて試料収納
部を形成させ該筒状体上下に蓋体を設けると共
に、一方の蓋体に加圧液の出入孔を出口孔にはバ
ルブを設けて形成させ、筒状体内には筒状体に内
接して押圧子を摺動自在に設ける一方他の蓋体は
着脱自在に為し且つバルブを備えた試料排出口を
設けたので液体クロマトグラフの送液部と着脱自
在と為すことが出来、装置自体に駆動部を構成す
る必要がなく、送液部を動力源とするので極めて
簡単で小型軽量の装置とすることが出来る。この
結果、装置全体を例えば氷にて冷却する操作も簡
単に行うことが出来極めて便利である。又低廉な
価格にて提供できる他、収納設置場所も狭くて済
み設置コストが低廉である等実用効果著大であ
る。F. Functions and Effects of the Device According to the invention as described above, a sample storage section is formed by a cylindrical body, lids are provided on the top and bottom of the cylindrical body, and an inlet and an outlet for pressurized liquid are provided in one of the lids. A valve is provided in the hole, and a presser is provided in the cylindrical body so as to be slidable therein, while the other lid is detachable and has a sample discharge port equipped with a valve. Because of this, it can be attached and detached from the liquid feeding part of the liquid chromatograph, and there is no need to configure a driving part in the device itself, and since the liquid feeding part is the power source, the device is extremely simple, small and lightweight. I can do it. As a result, the operation of cooling the entire apparatus with ice, for example, can be easily performed, which is extremely convenient. In addition, it can be provided at a low price, and has great practical effects such as the storage space is small and the installation cost is low.
第１図は、本考案一実施例縦断側面図である。
１……筒状体、２……試料室、３……蓋体、４
……袋ナツト、５……流入孔、６……加圧管の螺
入部、７……出口孔、８……排出管の螺入部、９
……バルブ、１０……Ｏリング、１１……押圧
子、１２……Ｏリング、１３……Ｏリング、１４
……引出棒、１５……通孔、１６……蓋体、１７
……Ｏリング、１８……袋ナツト、１９……試料
排出口、２０……排出管の螺入部、２１……バル
ブ、２２……Ｏリング。
FIG. 1 is a longitudinal sectional side view of one embodiment of the present invention. 1... Cylindrical body, 2... Sample chamber, 3... Lid, 4
... Cap nut, 5 ... Inflow hole, 6 ... Threaded part of pressure pipe, 7 ... Outlet hole, 8 ... Threaded part of discharge pipe, 9
... Valve, 10 ... O-ring, 11 ... Presser, 12 ... O-ring, 13 ... O-ring, 14
...Drawer rod, 15...Through hole, 16...Lid body, 17
... O-ring, 18 ... Cap nut, 19 ... Sample discharge port, 20 ... Threaded part of discharge pipe, 21 ... Valve, 22 ... O-ring.
Claims (1)
に蓋体を設けると共に、一方の蓋体に加圧液の出
入孔を出口孔にはバルブを設けて形成させ、筒状
体内には筒状体に内接して押圧子を摺動自在に設
ける一方他の蓋体は着脱自在に為し且つバルブを
備えた試料排出口を設けたことを特徴とする細胞
破壊装置。 A sample storage section is formed from a cylindrical body, lids are provided on the top and bottom of the cylindrical body, and one lid is formed with an inlet/outlet hole for the pressurized liquid and an outlet hole is provided with a valve. 1. A cell disruption device, characterized in that a presser is slidably provided inscribed in a cylindrical body, while the other lid is detachable and is provided with a sample outlet equipped with a valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5174787U JPH0513198Y2 (en) | 1987-04-06 | 1987-04-06 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5174787U JPH0513198Y2 (en) | 1987-04-06 | 1987-04-06 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63163460U JPS63163460U (en) | 1988-10-25 |
| JPH0513198Y2 true JPH0513198Y2 (en) | 1993-04-07 |
Family
ID=30876293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5174787U Expired - Lifetime JPH0513198Y2 (en) | 1987-04-06 | 1987-04-06 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0513198Y2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195038B (en) * | 2014-09-11 | 2016-06-15 | 广州聚能纳米生物科技股份有限公司 | The double; two high pressure cylinder homogenization integrated device of coaxial line |
| EP3162885B1 (en) * | 2014-09-11 | 2018-07-04 | Guang Zhou Jueng Nano&Bio Technology Co., Ltd | Ultrahigh-pressure homogenizing integrated device and cell disruptor |
-
1987
- 1987-04-06 JP JP5174787U patent/JPH0513198Y2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63163460U (en) | 1988-10-25 |
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