JPH0477415A - Dental calcus preventing composition - Google Patents

Abstract

PURPOSE:To obtain dental calcus-preventing composition having excellent dental calcus formation-preventing action and high safety even in continuous use for a long time containing casein phosphopeptide as an active ingredient. CONSTITUTION:Casein phosphopeptide used in an amount of >=0.01wt.%, preferably 0.1-5wt.% to total of the composition and other well-known excipient, auxiliary, flavor, antibacterial agent or antiseptic agent, etc., are suitably mixed, then prepared to afford the objective composition. Said composition can be used in adding to dentifrice, mouth wash, a troche, a gargle, an ointment or chewing gum, etc. Casein phosphopeptide suppresses precipitation of calcium phosphate regarded as a cause of dental calcus formation, suppresses the transformation from amorphous calcium phosphate to hydroxyapatite and further effectively suppresses the crystal growth of hydroxyapatite.

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to an anti-tartar composition, and more particularly, to an anti-tartar composition that can be added to oral compositions such as toothpaste, mouthwash, troches, etc. to suppress the formation of tartar. The present invention relates to an anti-tartar composition.

<Prior Art> Due to its rough surface, dental calculus is known to be a mechanical irritating factor for the gums and to be a cause of gingivitis and periodontitis. Although the details of tartar formation are not necessarily clear, calcium and phosphorus supplied from saliva and exudate are deposited on organic substrates such as bacteria and salivary glycoproteins that make up plaque, and this crystallizes. It can be understood as a calcification phenomenon of plaque. On the other hand, the concentration of calcium and phosphorus present in saliva and serum is supersaturated with respect to calcium phosphate, which is the main component of teeth and bones. It is thought that this is because it exists. Therefore, in order to suppress tartar formation, we conducted research on the inhibitory effects of sodium polyphosphate on tartar formation using sodium pyrophosphate (PP) and sodium tripolyphosphate (TPP), for example, in ran vitro and animal experiments.'' (Mikio Miyake) , Fumio Osato, Journal of Periodontology Vol. 30, No. 3, p. 860-867), etc. have been proposed.

Furthermore, casein phosphopeptides have been conventionally known as calcium absorption promoting factors, and for example, peptides derived from β-casein and peptides derived from α-casein are known. However, at present, it is not known that the casein phosphopeptide has an effect of preventing dental calculus formation.

<Problems to be Solved by the Invention> Therefore, an object of the present invention is to provide an anti-tartar composition that has an excellent effect of preventing tartar formation and is highly safe even during continuous use over a long period of time.

<Means for Solving the Problems> According to the present invention, there is provided an anti-tartar composition characterized by containing casein phosphopeptide as an active ingredient.

The present invention will be explained in more detail below.

In the present invention, casein phosphopeptide is used as an active ingredient.
e) is a component that suppresses the precipitation of calcium phosphate that occurs at the initial stage of tartar formation, suppresses the transition of hydroxyapatite from the primary amorphous calcium phosphate, and further effectively suppresses the crystal growth of hydroxyapatite, This inhibition of calcium phosphate precipitation is thought to be due to casein phosphopeptide chelating with Ca'' ions and suppressing the formation of calcium phosphate. It adsorbs and blocks crystal growth points, suppressing crystal growth.

Furthermore, casein phosphopeptide is a peptide made from casein and is an extremely safe substance that does not cause any irritation to the oral cavity and does not stimulate bone demineralization like tripolyphosphate and pyrophosphate. It is.

The casein phosphopeptide used in the present invention can be obtained by a known method, for example, by hydrolyzing β-casein with crystalline trypsin and purifying it [R, F, Peterson.

L, V, Nouman and T, L, McMeek
in, Journal of theA+++eric
an Che+*1cal 5ociety 8
0 9595-99 (195 or R, Na1to an
d H, 5uzuki, Agricultural Bi
Logical Chemistry 38 1
543-1545 (1974)], various acid caseins,
A method in which sodium caseinate, calcium caseinate, etc. are used as raw materials, treated with trypsin or an enzyme containing trypsin, and then fractionated (Japanese Patent Laid-Open No. 159792
In addition, for example, the product name "Meiji CPP-I" can be prepared according to
J (CPP content 12% by weight or more), [Meiji cpp-2
J (CPP content 12% by weight or more), r Meiji CPP
Commercially available products such as -31 (CPP content: 85% by weight or more) (manufactured by Meiji Seika Co., Ltd.) can also be used as they are.

In the tartar prevention composition of the present invention, the content of casein phosphopeptide used as an active ingredient is not particularly limited as long as it is at least an amount that can prevent tartar formation.
0.01% by weight or more based on the entire composition, preferably 0
．． 1 to 5% by weight is sufficient6 In the present invention, components that can be included other than casein phosphopeptide include, for example, known excipients, adjuvants, fragrances, antibacterial agents, preservatives, etc. can.

The mode of action of the anti-tartar composition of the present invention is, for example, known oral preparations, specifically dentifrices, mouthwashes,
It can be used as a lozenge, gargle solution, chewing gum, etc.

<Effects of the Invention> Since the tartar prevention composition of the present invention contains casein phosphopeptide as an active ingredient, it can effectively prevent tartar formation, which is known as the cause of gingivitis.Moreover, casein phosphopeptide is a natural product. Since it is extremely safe, it is extremely useful as oral hygiene products and food additives.

<Examples> The present invention will be explained in more detail below using test examples and examples, but the present invention is not limited thereto.

In order to investigate the inhibitory effects of calcium phosphate precipitate formation, the transition from amorphous calcium phosphate to hydroxyapatite, and the crystal growth of hydroxyapatite, which are considered to be the causes of dental calculus formation, casein phosphopeptide was used. Commercially available product name “Meiji CPP-I”
J, rMeiji CPP-2", "Meiji CPP-34' (
The above was carried out as follows using Meiji Seika Co., Ltd.).

(a) Calcium phosphate precipitation inhibition test First, 1 of 16
0 - In a test tube, each casein phosphopeptide and, as a comparative test, tripolyphosphoric acid, which is conventionally known as an active ingredient for preventing tartar formation, were added to the entire test solution.
．． 05wt%, O, 1wt%, 0.25wt%, 0,
Calcium phosphate solution (p
After mixing with a mixer, 0.25 ml of calcium chloride solution was added and mixed to make the entire test solution 5 mQ. Each of the obtained solutions was
-550n using 1100J (Hitachi, Ltd.)
The absorbance at m was measured.

Also during this measurement, each solution was stirred with a mixer, and the measurement was carried out in a state in which the generated precipitate was uniformly dispersed. The relationship between the obtained absorbance and each active ingredient is shown in Figure 1.
In the figure, the higher the absorbance, the higher the effect of suppressing precipitation of calcium phosphate. Therefore, from the results in Figure 1,
Casein phosphopeptide was found to be more effective in inhibiting calcium phosphate precipitation than conventional tripolyphosphate at a trace amount.

(b) Hydroxyapatite formation inhibition test 2 mQ of O, 1M potassium phosphate aqueous solution and the product name "
Meiji CPP-IJ (casein phosphopeptide content: 12% by weight), “Meiji CPP-2J (casein phosphopeptide content: 12% by weight).

"Meiji CPP-34 (casein phosphopeptide content 8
5% by weight) and for comparison, tripolyphosphoric acid (TPP) was 0.02.0% relative to the entire sample solution (22mQ).
．． 04. After mixing to 0.08% by weight, 2m
Q 0.1M aqueous calcium chloride solution was added. For the reaction, the pH was measured from the time the calcium chloride was added using a product named "rHM-7E PH Tsume" (manufactured by Toa Denpa Co., Ltd.), and the time for hydroxyapatite formation was measured. The results are shown in Table 1. In addition, if casein phosphopeptide or tripolyphosphate is not added, 1
Hydroxyapatite was formed in 6 minutes.

From the results in Table 1, it can be estimated that casein phosphopeptide has the same or greater effect of suppressing hydroxyapatite formation than tripolyphosphate. In addition, the product name "Meiji CPP-IJ" and "Meiji CPP-24"
The formation time of hydroxyapatite is considerably faster than that of tripolyphosphate, but this is because the concentration of the active ingredient, casein phosphopeptide, is 12% by weight, which is approximately 1/8 of the concentration of tripolyphosphate; That is, it can be seen that even greater effects can be obtained when Meiji CPP-3J is used.

Table 1 (c) Hydroxyapatite crystal growth inhibition test containing 0.05M common salt and 1.06mM calcium chloride.
．． 63mM potassium phosphate buffer (pH 7,4) 20-
The product name "Meiji CPP-IJ", "Meiji CPP-2J"
Add rMeiji CPP-3J or TPP to a concentration of 0.5 to 5 PPM, and further use the product name rN as a seed crystal.
251 kg of hydroxyapatite oH-0252°Type J (manufactured by Sigma Corporation) was added under stirring. The phosphorus concentration was measured by collecting each supernatant every hour and quantifying it by molybdenum blue colorimetric method. The results are shown in Figures 2a''-d, respectively.

From the results shown in Figures 2a to 2d, it was found that casein phosphopeptide has the same effect of inhibiting hydroapatite crystal growth as tripolyphosphate.

[11L2] - Examples in which each of the casein phosphopeptides that showed calcium phosphate inhibitory activity in the test examples were blended are shown below.

(Margin below) (Example 1) Muj4 cupcake (Example 4) Chi' 22 (Example 2) Mouthwash (Example 5) drum (Example 3) 上 o -f 赳

[Brief explanation of drawings]

FIG. 1 is a graph showing the concentration and transmittance of casein phosphopeptide and tripolyphosphoric acid conducted in test examples;
The figure is a graph showing the time and phosphorus concentration at each concentration using the product name "Meiji CPP-1" in the test example,
Figure b is a graph showing time and phosphorus concentration at each concentration using Meiji CPP-2J, also under the trade name, and Figure 2C is a graph showing time and phosphorus concentration at each concentration using Meiji CPP-3J, also under the trade name. Figure 2d is a graph showing the time and phosphorus concentration at each concentration using tripolyphosphoric acid.

Claims (1)

[Claims] An anti-tartar composition characterized by containing casein phosphopeptide as an active ingredient.