JPH0469569A - Method and device for dispensing sample for liquid chromatography - Google Patents
Method and device for dispensing sample for liquid chromatographyInfo
- Publication number
- JPH0469569A JPH0469569A JP2180565A JP18056590A JPH0469569A JP H0469569 A JPH0469569 A JP H0469569A JP 2180565 A JP2180565 A JP 2180565A JP 18056590 A JP18056590 A JP 18056590A JP H0469569 A JPH0469569 A JP H0469569A
- Authority
- JP
- Japan
- Prior art keywords
- column
- injector
- pump
- preheater
- eluent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 11
- 238000004811 liquid chromatography Methods 0.000 title claims description 9
- 239000003480 eluent Substances 0.000 claims description 26
- 238000004064 recycling Methods 0.000 claims description 17
- 238000000926 separation method Methods 0.000 claims description 15
- 238000004891 communication Methods 0.000 description 6
- 238000010828 elution Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- YXWWHNCQZBVZPV-UHFFFAOYSA-N 2'-methylacetophenone Chemical compound CC(=O)C1=CC=CC=C1C YXWWHNCQZBVZPV-UHFFFAOYSA-N 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- FSPSELPMWGWDRY-UHFFFAOYSA-N m-Methylacetophenone Chemical compound CC(=O)C1=CC=CC(C)=C1 FSPSELPMWGWDRY-UHFFFAOYSA-N 0.000 description 2
- 238000004262 preparative liquid chromatography Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Description
【発明の詳細な説明】
イ、産業上の利用分野
本発明は、液体クロマトグラフィーの試料分取方法及び
装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION A. Field of Industrial Application The present invention relates to a sample collection method and apparatus for liquid chromatography.
口、従来の技術
液体クロマトグラフィーに於て1分析の再現性を良くす
るために、カラムをエアーオープンの中に入れ、温度を
一定にして分析を行う方法がある。BACKGROUND OF THE INVENTION In liquid chromatography, in order to improve the reproducibility of analysis, there is a method in which the column is placed in an air-open environment and the temperature is kept constant.
更に、最近ではこの方法が分取液体クロマトグラフィー
にも応用されるようになってきた。Furthermore, recently this method has been applied to preparative liquid chromatography.
従来の液体クロマトグラフィーの試料分取装置就中リサ
イクル分取装置としては第七図に示すように、リサイク
ルバルブにポンプと検知器とを連結し、ポンプにはプレ
ヒーター、インジェクターカラムを順次連結し、該プレ
ヒーター、インジェクター、カラムをエアーオープン内
に収納してあり、エアーオープンと検知器を連結した装
置が使用されている。As shown in Figure 7, a conventional liquid chromatography sample separation device, especially a recycling separation device, connects a pump and a detector to a recycle valve, and connects a preheater and an injector column to the pump in sequence. A device is used in which the preheater, injector, and column are housed in an air vent, and the air vent is connected to a detector.
ハ、解決すべき問題点
前記の分取液体クロマトグラフィーで用いるカラムは内
径が太いために、エアーオープンではカラムの中心部ま
で温めることが出来ずに、カラム内で周辺部と中心部の
間に温度勾配が出来てしまう。その為溶出ピークがブロ
ードになったり、ピーク割れを起こすことがある。この
ため分取カラムをエアーオープンで温度制御する場合は
、溶離液をカラムと同じ温度に余熱してから(プレヒー
ト)カラムに導入する必要がある。C. Problems to be solved Because the columns used in the preparative liquid chromatography mentioned above have a large inner diameter, it is not possible to heat the center of the column with air opening, and there is a gap between the periphery and the center within the column. This creates a temperature gradient. Therefore, the elution peak may become broad or peak cracking may occur. For this reason, when controlling the temperature of a preparative column with air open, it is necessary to preheat the eluent to the same temperature as the column (preheat) before introducing it into the column.
一方リサイクル分取に於て効率良く分取を行うには、リ
サイクル系内(ポンプ→カラム→検知器→ポンプ→・・
・・・)の装置及び配管のデッドボリュームをなるべく
小さくしてカラム外の拡散を最小にする必要がある。On the other hand, in order to perform efficient preparative separation in recycling, it is necessary to
...) It is necessary to minimize the dead volume of the equipment and piping to minimize diffusion outside the column.
従ってリサイクル分取にエアーオープンを使おうとした
場合、プレヒートの部分が大きなデッドボリュームにな
ってしまいリサイクル効率が悪くなってしまう。Therefore, when attempting to use air open for recycling separation, the preheating portion becomes a large dead volume, resulting in poor recycling efficiency.
ニ5問題点を解決するだめの手段
そこで本発明においては、分取カラムをエアーオープン
で温度制御し、溶離液をカラムと同し温度にプレヒート
してカラムに導入し溶出ピークを狭く且ピークをシャー
プにすることが出来ると共に、一方ではプレ上−1〜部
分はリサイクル系から遮断し、リサイクル効率を極めて
良くぜんとする矛盾した目的を解決するための方法及び
装置を提案するもので、カラムをエアーオープン内で温
度制御し、溶離液をブレヒートして導入する方法に於て
、リサイクル系に試料を注入してプレヒーターならびに
インジェクターを通して溶離液を流す工程と目的成分が
溶出し始めた段階でプレヒーターならびにインジェクタ
ーをリサイクル系から外して試料溶離液を流す工程とよ
り成る液体クロマトグラフィーの試料分取方法、及びリ
サイクルバルブを介してポンプと検知器を連結させ、ポ
ンプと検知器間にプレヒーター、インジェクター、カラ
ムを連結し、且エアーオープン内に収納させた試料分取
装置に於て、一端をポンプと他をプレヒーター、インジ
ェクター、カラムと夫々連結した切替バルブを設け、ポ
ンプとプレヒーター、インジェクター、カラムを順次結
ぶ回路とポンプとカラムを結ぶ回路とに切替自在と為し
たことを特徴とする液体クロマトグラフィーの試料分取
装置を提案するものである。5. Means to Solve Problems Therefore, in the present invention, the temperature of the preparative column is controlled with air open, and the eluent is preheated to the same temperature as the column and introduced into the column, thereby narrowing the elution peak and suppressing the peak. This paper proposes a method and apparatus for solving the contradictory objectives of sharpening the column, while at the same time isolating the upper part of the column from the recycling system and achieving extremely high recycling efficiency. In the method of introducing the eluent by controlling the temperature in an air open and preheating the eluent, there is a process in which the sample is injected into the recycling system and the eluent is flowed through a preheater and an injector, and a preheating process is performed at the stage when the target component begins to elute. A liquid chromatography sample separation method comprising a step of removing a heater and an injector from a recycling system and flowing a sample eluent, and connecting a pump and a detector via a recycle valve, and a preheater and a preheater between the pump and the detector. In a sample separation device in which an injector and a column are connected and housed in an air opening, a switching valve is provided that connects the pump at one end and the preheater, injector, and column at the other end, and connects the pump, preheater, and injector. The present invention proposes a sample separation device for liquid chromatography, which is characterized in that it can be freely switched between a circuit that connects columns sequentially and a circuit that connects a pump and a column.
ホ、実施例 以下図に示す一実施例により本発明の詳細な説明する。E, Example The present invention will be explained in detail below with reference to an embodiment shown in the drawings.
上はリサイクルバルブで、溶離液9の吸入口1g、ポン
プ2との連通孔12、検知器7との連通孔17及び排出
孔1□を備えている。ポンプ2は突出口2工を切替バル
ブ3の接続口3.に接続しである。該切替バルブ3の接
続口3□にはプレヒーターとしてのプレヒートチューブ
4一端を連通させ、その他端はインジェクター5の切替
口5.に接続しである。該インジェクター5にはループ
52を接続し切替自在に為してあり、その一端は切替口
5□を介して切替バルブ3の接続口3.に接続しである
。6はカラムで一端を切替バルブ3の接続口34と接続
して、その他端は検知器7に接続し、検知fM7はその
一端をリサイクルバルブ1の連通孔17と接続しである
。The upper part is a recycle valve, which has an inlet 1g for the eluent 9, a communication hole 12 with the pump 2, a communication hole 17 with the detector 7, and a discharge hole 1□. The pump 2 has two protruding ports and a connecting port 3 of the switching valve 3. It is connected to. One end of a preheat tube 4 as a preheater is communicated with the connection port 3□ of the switching valve 3, and the other end is connected to the switching port 5 of the injector 5. It is connected to. A loop 52 is connected to the injector 5 so that it can be switched freely, and one end of the loop 52 is connected to the connection port 3. of the switching valve 3 via the switching port 5□. It is connected to. A column 6 has one end connected to the connection port 34 of the switching valve 3, the other end connected to the detector 7, and the detection fM7 has one end connected to the communication hole 17 of the recycle valve 1.
上記の切替バルブ3、プレヒートチューブ4、インジェ
クター5及びカラム6はエアーオープン8内に収納させ
である。又、切替バルブ3は図面に於ては4方切替バル
ブを用いているが、6方切替バルブその他適宜のものを
使用できること勿論である。The above-described switching valve 3, preheat tube 4, injector 5, and column 6 are housed in the air opening 8. Further, although a four-way switching valve is used as the switching valve 3 in the drawings, it is of course possible to use a six-way switching valve or any other suitable valve.
以下、その使用並びに作動について説明すれば、インジ
ェクター5に常法によりニードルポートを経てシリンジ
により任意量を或はループにループ容量を注入する等に
よりサンプルを注入する。The use and operation thereof will be explained below. A sample is injected into the injector 5 in a conventional manner by injecting an arbitrary amount with a syringe through a needle port or by injecting a loop volume into a loop.
リサイクル目的成分が溶出するまではプレヒーター並び
にインジェクターを通して溶離液を流通させる。即ち、
溶離液9と吸入口1gとを連通させ、連通孔12よりポ
ンプ2の吸引作動により溶離液9を吸引し、突出口2□
より切替バルブ3の接続口31に送り込む。このとき、
切替バルブ3は接続口31と接続口3□は連通しており
、溶離液9は接続口31、同32を介してプレヒートチ
ューブ4に流入してヒーターにより温められる。そして
、インジェクター5に送られる。次いで、切替口53よ
りそのループ5□を経てサンプルと共に切替口51を経
て切替バルブ3の接続口31、同接続口34よりカラム
6内に送入される。この温められた溶離液9の通過によ
り、カラム6は温められる。勿論、インジェクター5、
カラム6等はエアーオープン8により温められているが
、カラム6はオーブンにより中心までは温められないの
で、温められた溶離液9を順次流すことにより、内外差
を無くすまで温めることが出来る。カラム6により流出
せしめられた溶離液9は検知器7を経てリサイクルバル
ブ1に戻り排出孔l工より排出される。The eluent is circulated through the preheater and injector until the target component for recycling is eluted. That is,
The eluent 9 and the suction port 1g are communicated, and the eluent 9 is sucked through the communication hole 12 by the suction operation of the pump 2, and the eluent 9 is drawn into the eluent 2□.
Then feed it into the connection port 31 of the switching valve 3. At this time,
In the switching valve 3, the connection port 31 and the connection port 3□ communicate with each other, and the eluent 9 flows into the preheat tube 4 through the connection ports 31 and 32 and is heated by the heater. Then, it is sent to the injector 5. Next, the sample is sent through the switching port 53 through the loop 5□, through the switching port 51, through the connection port 31 of the switching valve 3, and into the column 6 through the connection port 34. The column 6 is warmed by the passage of the warmed eluent 9. Of course, injector 5,
Although the column 6 and the like are heated by the air open 8, the column 6 cannot be heated to the center by the oven, so by sequentially flowing the heated eluent 9, it can be heated until the difference between the inside and outside is eliminated. The eluent 9 discharged from the column 6 passes through the detector 7, returns to the recycle valve 1, and is discharged from the discharge hole 1.
以上の動作により溶離液9と共に流されるサンプルの目
的成分が溶出し始めた段階で、リサイクルバルブ上を切
替え、連通孔1□と連通孔I7を接続させ、循環系を形
成させる。と同時に切替バルブ3を切替え、接続口31
と接続口34を接続させて、接続口31と接続口3□及
び接続口3.と接続口34とは遮断させる。When the target component of the sample flowing together with the eluent 9 begins to elute through the above operations, the recycle valve is switched to connect the communication hole 1□ and the communication hole I7 to form a circulation system. At the same time, switch the switching valve 3 and connect the connection port 31.
and connection port 34, and connect connection port 31, connection port 3□, and connection port 3. and the connection port 34 are cut off.
この結果、リサイクルバルブ1、ポンプ2、切替バルブ
3、カラム6、検知器7、リサイクルバルブ1のリサイ
クル系が形成される。As a result, a recycle system including the recycle valve 1, the pump 2, the switching valve 3, the column 6, the detector 7, and the recycle valve 1 is formed.
又、他の経路としては、第三図、第四図に示すようにプ
レヒートチュブ4を切替バルブ3の接続口3□、同33
間に接続し、接続口34はインジェクター5の切替口5
□に接続しておく。インジェクター5の他の切替口5.
はカラム6に接続しておく。又。In addition, as another route, as shown in Figures 3 and 4, the preheat tube 4 is connected to the connection port 3□, 33 of the switching valve 3.
The connection port 34 is connected to the switching port 5 of the injector 5.
Connect to □. Other switching ports of injector 5 5.
is connected to column 6. or.
インジェクター5にはその切替口5□、同51間にルー
プ5□を切替自在に設定しである。そして、切替バルブ
3を切替えた時、接続口3□と接続口34は連通し、切
替バルブ3の接続口3□と接続口33とは遮断される。The injector 5 has a switching port 5□ and a loop 5□ between the switching port 5□ and the switching port 51 so as to be freely switchable. When the switching valve 3 is switched, the connection port 3□ and the connection port 34 are communicated with each other, and the connection port 3□ of the switching valve 3 and the connection port 33 are cut off.
さらにインジェクター5に於てもループ5□との接続を
解く。Furthermore, injector 5 is also disconnected from loop 5□.
この場合にも上記同様のリサイクル系が形成される。In this case as well, a recycling system similar to the above is formed.
へ、実施例
カラムInertsil PREP−ODS250 X
20+nm1.D。Example column: Inertsil PREP-ODS250
20+nm1. D.
溶離液 アセトニトリル/水=674
流 速 10m Q /ll1in検 出
UV254nm
温 度 40℃
注入試料
1、、 m−Methyl acetophenone
2、 o−Methyl acetophenone
以上により第一図により実施した結果のクロマトグラム
を第五図に示す。Eluent Acetonitrile/Water = 674 Flow rate 10m Q/ll1in detection
UV254nm Temperature 40℃ Injection sample 1, m-Methyl acetophenone
2. o-Methyl acetophenone
FIG. 5 shows the chromatogram obtained as a result of carrying out the experiment according to FIG. 1 above.
又、第七図により実施した結果のクロマトグラムを第六
図に示す。In addition, the chromatogram of the results obtained in accordance with FIG. 7 is shown in FIG.
この結果、前者では11回のリサイクルによっても、二
つの成分の分離はまだ不完全であるのに対し、後者では
7回のリサイクルによって二つの成分の分離が完成して
いる。As a result, in the former case, the separation of the two components is still incomplete even after 11 times of recycling, whereas in the latter case, the separation of the two components is completed after 7 times of recycling.
ト0発明の効果
上記の如き本発明によれば、カラムをエアーオープン内
で温度制御し、溶離液をプレヒートして導入する方法に
於て、リサイクル系に試料を注入してプレヒーターなら
びにインジェクターを通して溶離液を流す工程と目的成
分が溶出し始めた段階でプレヒーターならびにインジェ
クターをリサイクル系から外して試料溶離液を流す工程
とより成り、及びリサイクルバルブを介してポンプと検
知器を連結させ、ポンプと検知器間にプレヒータ、イン
ジェクター、カラムを連結し、且エアーオープン内に収
納させた試料分取装置に於て、端をポンプと他をプレヒ
ーター、インジェクターカラムと夫々連結した切替バル
ブを設け、ポンプとプレヒーター、インジェクター、カ
ラムを順次結ぶ回路とポンプとカラムを結ぶ回路とに切
替自在と為したので、カラムは溶離液により予め加温さ
れ、溶離液と同じ温度に温められ、目的成分が溶出せし
めた段階にてプレヒーター、インジェクターを除いた循
環系を形成させるため、温度差による溶出ピークのブロ
ード化やピーク割れを防ぎ、ピークを鮮明に出すことが
出来、然もデッドボリュームの遮断によりリサイクル効
果を向上させることが出来る。然も構成も極めて簡単で
廉価である等実用効果著大である。Effects of the Invention According to the present invention as described above, in the method of controlling the temperature of the column in an air-open environment and preheating the eluent before introducing it, the sample is injected into the recycling system and passed through the preheater and injector. The process consists of the step of flowing the eluent, and the step of removing the preheater and injector from the recycling system and flowing the sample eluent at the stage when the target component begins to elute.Then, the pump and detector are connected via the recycle valve, and the pump A preheater, an injector, and a column are connected between the sensor and the detector, and in a sample separation device housed in an air open, a switching valve is installed, with one end connected to the pump and the other end connected to the preheater and the injector column, respectively. The circuit can be switched between a circuit that connects the pump, preheater, injector, and column in sequence, and a circuit that connects the pump and column, so the column is preheated by the eluent and heated to the same temperature as the eluent, allowing the target components to be absorbed. By forming a circulation system excluding the preheater and injector at the stage of elution, it is possible to prevent elution peaks from becoming broad or peak cracking due to temperature differences, and to produce clear peaks, while blocking dead volume. The recycling effect can be improved. Moreover, the structure is extremely simple and inexpensive, and the practical effects are great.
第一図、第二図は本発明一実施例説明図、第三図、第四
図は本発明化実施例説明図、第五図は従来例により得た
るクロマトグラム、第六図は本発明実施例により得たる
クロマトグラム、第七図はuI!来例説明図である。
、・1・・・リサイクルバルブ、2・・・ポンプ、3・
・・切替バルブ、6・・・カラム、7・・・検知器1゜
事件の表示
平成 2年特許願第180565号
2゜
発明の名称
液体クロマトグラフィーの試料分取方法及び装置3、補
正をする者
事件との関係 特許出願人
住 所 東京都新宿区歌舞伎町2丁目4番10号氏 名
(名称〕 ガスクロ工業株式会社代表者 森 憲
司
4、代理人〒160
住 所 東京都新宿区西新宿7丁目3番10号5゜Figures 1 and 2 are illustrations of an embodiment of the present invention, Figures 3 and 4 are illustrations of an embodiment of the invention, Figure 5 is a chromatogram obtained by the conventional example, and Figure 6 is an illustration of the invention. The chromatogram obtained in the example, Figure 7, shows uI! It is an explanatory diagram of the next example. ,・1... Recycle valve, 2... Pump, 3.
...Switching valve, 6...Column, 7...Detector 1゜Indication of incident 1990 Patent Application No. 180565 2゜Name of invention Liquid chromatography sample separation method and device 3, correction Patent applicant address 2-4-10 Kabukicho, Shinjuku-ku, Tokyo Name Gascro Kogyo Co., Ltd. Representative Kenji Mori 4, agent 160 Address Nishi-Shinjuku, Shinjuku-ku, Tokyo 7-3-10 5゜
Claims (2)
をプレヒートして導入する方法に於て、リサイクル系に
試料を注入してプレヒーターならびにインジェクターを
通して溶離液を流す工程と目的成分が溶出し始めた段階
でプレヒーターならびにインジェクターをリサイクル系
から外して試料溶離液を流す工程とより成る液体クロマ
トグラフィーの試料分取方法。(1) In a method in which the temperature of the column is controlled in an air-open environment and the eluent is preheated and introduced, the sample is injected into the recycling system and the eluent is flowed through the preheater and injector, and the target component is eluted. A sample separation method for liquid chromatography that consists of the step of removing the preheater and injector from the recycling system and flowing the sample eluent at the beginning.
させ、ポンプと検知器間にプレヒーター、インジェクタ
ー、カラムを連結し、且エアーオープン内に収納させた
試料分取装置に於て、一端をポンプと他をプレヒーター
、インジェクター、カラムと夫々連結した切替バルブを
設け、ポンプとプレヒーター、インジェクター、カラム
を順次結ぶ系路とポンプとカラムを結ぶ系路とに切替自
在と為したことを特徴とする液体クロマトグラフィーの
試料分取装置。(2) In a sample separation device in which a pump and a detector are connected via a recycle valve, a preheater, an injector, and a column are connected between the pump and the detector, and the sample separation device is housed in an air opening, one end is It is characterized by a switching valve that connects the pump and others to the preheater, injector, and column, respectively, so that it can be freely switched between a system that sequentially connects the pump, preheater, injector, and column, and a system that connects the pump and column. Liquid chromatography sample separation device.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2180565A JP2573884B2 (en) | 1990-07-10 | 1990-07-10 | Liquid chromatography sample separation method and apparatus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2180565A JP2573884B2 (en) | 1990-07-10 | 1990-07-10 | Liquid chromatography sample separation method and apparatus |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH0469569A true JPH0469569A (en) | 1992-03-04 |
JP2573884B2 JP2573884B2 (en) | 1997-01-22 |
Family
ID=16085504
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2180565A Expired - Lifetime JP2573884B2 (en) | 1990-07-10 | 1990-07-10 | Liquid chromatography sample separation method and apparatus |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2573884B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20220357304A1 (en) * | 2021-05-04 | 2022-11-10 | Waters Technologies Corporation | Compact liquid chromatography system providing thermal management and detection |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59172375U (en) * | 1983-04-30 | 1984-11-17 | 株式会社島津製作所 | Ultra-high performance liquid chromatograph |
JPS6267449A (en) * | 1985-09-20 | 1987-03-27 | Hitachi Ltd | Liquid chromatograph |
JPS62190463A (en) * | 1986-02-17 | 1987-08-20 | Hitachi Ltd | Recycle liquid chromatography and recycle liquid chromatograph |
JPS62199674U (en) * | 1986-06-11 | 1987-12-19 |
-
1990
- 1990-07-10 JP JP2180565A patent/JP2573884B2/en not_active Expired - Lifetime
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59172375U (en) * | 1983-04-30 | 1984-11-17 | 株式会社島津製作所 | Ultra-high performance liquid chromatograph |
JPS6267449A (en) * | 1985-09-20 | 1987-03-27 | Hitachi Ltd | Liquid chromatograph |
JPS62190463A (en) * | 1986-02-17 | 1987-08-20 | Hitachi Ltd | Recycle liquid chromatography and recycle liquid chromatograph |
JPS62199674U (en) * | 1986-06-11 | 1987-12-19 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20220357304A1 (en) * | 2021-05-04 | 2022-11-10 | Waters Technologies Corporation | Compact liquid chromatography system providing thermal management and detection |
Also Published As
Publication number | Publication date |
---|---|
JP2573884B2 (en) | 1997-01-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP3868899B2 (en) | Liquid chromatograph | |
US10955391B2 (en) | Multi-injection mode valve module | |
US7214313B2 (en) | Liquid chromatograph | |
US11573212B2 (en) | Flow channel mechanism and liquid chromatograph including the same | |
JP3719407B2 (en) | Preparative liquid chromatograph | |
EP0051778A2 (en) | A device for vaporization injection in a gas-chromatographic column | |
Deans | An improved technique for back-flushing gas chromatographic columns | |
US6402947B1 (en) | Interphase device for the direct coupling of liquid chromatography and gas chromatography | |
DE69128636T2 (en) | MULTIDIMENSIONAL ON-LINE CHROMATOGRAPHY SYSTEM WITH LARGE INJECTION CAPACITY FOR SUPERCRITICAL FLUID CHROMATOGRAPHY | |
US20060273012A1 (en) | Column with additional fluid introduction | |
KR20210141308A (en) | On-line system for improving detection level of analytes by liquid chromatography and analysis method using the same | |
JPH0469569A (en) | Method and device for dispensing sample for liquid chromatography | |
US20070023639A1 (en) | Liquid chromatographic apparatus | |
WO2018128836A1 (en) | Modifier stream elution of trap column for multidimensional compressible fluid-based chromatography | |
JPS6197567A (en) | Sample preprocessor | |
CN215250532U (en) | Protein chromatography device | |
US4271703A (en) | High temperature sampling system for liquid chromatography | |
US3352089A (en) | Method and apparatus for introducing samples into a gas chromatographic column | |
CN106841404B (en) | A kind of high temperature Comprehensive two-dimensional LC device and its application method | |
Dolan | The importance of temperature | |
JP6672781B2 (en) | Flow path mechanism and liquid chromatograph provided with the same | |
CN112180021B (en) | Liquid Chromatography System | |
EP3396372B1 (en) | Apparatus and method for recirculation of fluids including two 4-port 2-way-valves | |
JP2743124B2 (en) | Liquid chromatograph | |
JP3693358B2 (en) | Solvent mixing device |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081024 Year of fee payment: 12 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20091024 Year of fee payment: 13 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20091024 Year of fee payment: 13 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20101024 Year of fee payment: 14 |
|
EXPY | Cancellation because of completion of term | ||
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20101024 Year of fee payment: 14 |