JPH044820A - Plant contained in a container for admiration - Google Patents

Abstract

PURPOSE:To obtain the subject plant, composed of an observable container having a lid unit capable of free passage of oxygen gas and gaseous carbon dioxide without invasion of microorganisms, a germicide-containing culture medium and a plant for admiration and capable of completely preventing molds from growing or diseases from occurring. CONSTITUTION:The objective plant is composed of (A) a container (e.g. composed of glass), provided with a lid unit (e.g. composed of a synthetic resin), preventing microorganisms from invading and enabling free passage of oxygen gas and gaseous carbon dioxide and capable of transmitting at least light required for living of the plant and observing the interior thereof, (B) a germicide-containing culture medium in an amount of <=50% based on the volume of the aforementioned container and (C) the plant for admiration transplanted to the above-mentioned plant.

Description

[Detailed description of the invention] [Industrial application field] The present invention relates to containerized ornamental plants.

[Conventional technology]

Conventionally, containers have been provided with a lid that prevents microorganisms from entering the container, allows oxygen gas and carbon dioxide gas related to the metabolic physiology of plants to freely enter and exit the container, and transmits at least the light necessary for plants to inhabit. It is known that plants can be grown using containers that allow the inside to be inspected, but the above-mentioned lid is just an ideal, and in reality, microorganisms can invade over time and mold can grow. It was not possible to completely prevent the occurrence of this disease and the plants from contracting the disease.

〔the purpose〕

Therefore, an object of the present invention is to completely prevent the above-mentioned growth of mold and disease.

〔composition〕

The present invention provides (a) a lid body that prevents microorganisms from entering the container and allows oxygen gas and carbon dioxide gas related to the metabolic physiology of plants to freely enter the container; a container that transmits necessary light and allows the inside to be observed; (b) a preferably sterilized medium containing a sterilizing agent stored in the bottom of the container; (c) a preferably sterile medium The present invention relates to an ornamental plant in a container, which is characterized by being comprised of an ornamental plant that has been transplanted.

As the fungicide in the present invention, all known fungicides can be used, but in particular, benzimidazole fungicides (according to the Agricultural Handbook, benomyl agents, thiabendazole agents, carpendazole agents, and thiophanate compounds having a benzimidazole skeleton) are used. is a general term for thiophanate agents and thiophanate methyl agents.) is preferred. Specific compounds include 2'-methoxycarbonylaminobenzimidazole-
4'1-dodecylpenzole sulfonate, 2-benzimidazole.

(Left below) Other than benzimidazole fungicides, 3,3'-ethylenebis(tetra)hydro-4,6-dimethyl-2H-1,3,5-thiadiazine-2-thione, zinc ethylenebisdithiocarbamate, ethylene Manganese bisdithiocarbamate, bis-dimethyldithiocarbamoyl tinc ethylene bisdithiocarbamate, bis(dimethylthiocarbamoyl) disulfide [tetramethylthiuram disulfide], N-tetrachloroethylthio-4-cyclohexene-1,2-dicarboxyimide, N-) -lichloromethylthio 4-cyclohexene-1,2-dicarboxyimide, 5,6-cyhydro-2-methyl-1,4-oxatine-3-carboxyanilide-4,4-dioxide, tetrachloroisophthalonitrile , 2-(1-methylheptyl)-4,6-sinitrophenylcrotonate, 2,4-dinitro-6-
Secondary butylphenyl dimethyl acrylate, 5-ethoxy-3-trichloromethyl-1,2,4-thiadiazole, 2,4-dichloro-6-(0-chloroanilino)
-1,3,5-)-riazine, 2,3-dicyano-1,
Various fungicides include 4-dithioanthraquinone, tincpropylene bis(dithiocarbamate), and tinc(dimethyldithiocarbamoyl)ethylenediamine.

The culture medium that can be used in the present invention includes 80 to 99.5% water by weight,
A sugar content of 0 to 20% by weight and agar of 0 to 2% by weight are suitable. Typical media that correspond to this include Murashige & Skoog.
Medium, Linsmaier-Skoog
& Skoog medium, kano medium, white medium and Nitsch medium.
) media can be used, as well as modified media of these media.

The medium can be colored, but pale purple is particularly preferred.

The amount of medium is suitably 50% or less of the volume of the container. The container that can be used in the present invention is made of glass, synthetic resin, or the like. Examples of the synthetic resin include polycarbonate, polyester, polyethylene, polypropylene, and polyvinyl chloride.

The shape and structure of the container are not particularly limited, and are determined by taking into account the installation method for viewing, workability and aesthetics when transplanting tissue-cultured plants. For example, when viewing while standing, it is preferable that the bottom be flat and comfortable to sit on.

Specifically, examples include a container shaped like a normal wide-mouth bottle, a container shaped like a sphere with one part flattened, and a container shaped like a conventional animal or vehicle. . In addition, only the part of the container where the plant is present is transparent.

Other parts may not be transparent.

The lid body may be of any material, shape, or structure as long as it has the function of sealing the container and can withstand sterilization by autoclaving at approximately 120° C. for approximately 15 minutes. The material of the lid may be different from that of the container, may not be transparent, and may be a flexible material. For example, synthetic resin (polyethylene, polypropylene, polyurethane, etc.), metal (stainless steel, aluminum foil, etc.), glass, cotton, etc. can be used.

The shape and structure of the lid vary depending on the sealing method and material. For example, it can be sealed using the flexibility of synthetic resin, covered with aluminum foil, or sealed with a filter.

Further, screw threads can be formed in corresponding parts of the lid and the container so that the two can be screwed together. At this time, in either case, the container is not completely sealed with a lid, but it is necessary to prevent microorganisms such as bacteria and mold from in the environment from entering the container, and to prevent oxygen and carbon dioxide gases, which are related to the metabolic physiology of plants, from entering the container. The structure shall be such that it is sealed to the extent that it is possible to enter and exit the container naturally.

To make the medium sterile, fill the medium into a container and hold it at a temperature of 105-130 °C for 5-20 minutes, or
This can be carried out by aseptically dispensing a medium that has been heat sterilized, a medium that has been autoclaved, or a medium that has been sterilized by ultrafiltration into a sterilized container.

In the sealing operation described below, in the case of plastic containers, it is performed in a sterile environment such as a clean room or clean bench, and in the case of glass containers such as ampoules, it is melt-sealed with a gas flame. Bacteria contamination can be prevented.

The plants to which the present invention is applied are as follows.

fly trap Venus flytrap genus Venus flytrap Dionaea muscipula Ellis.

Coleus Coleus blumei Benth.

Anthrilum spp. Anehuriumandraeanum Lind.

Cymbidium Begonia tuberhybrida Voss
.

1ibiscus rasa-sinensis L
.

5aintpaulia 1onantha Wend
l.

Dendrobium moniliforme (L
,)Sw Dro
sera anglica Hudson.

Coleus Anthrilum Cymbidium Regal Begonia Hibiscus Centpaulia Serracoccus Ficus Ficus Malbind Rubber Ficus elastica Roxb+'Deeo
ra' Ficus Ficus 1yrata Warb.

[Examples and effects]

(1) Medium used Table-1 (S-2 medium) (2) Anti-fungal agent used ・Courtside QSP6g manufactured by Takeda Pharmaceutical Co., Ltd.

(Active ingredients: benzimidazole compounds and nitrogen-containing sulfur compounds) ・Suraoff [F] 620 manufactured by Takeda Pharmaceutical Co., Ltd. (Active ingredients: halogenated nitrogen-sulfur compounds) (3) Experimental method 1) Courtside 5P68 and Suraoff 620 was added to S-2 medium at 0.01 vol 1%, 0.1 vol 1% and 1 vol.
It was added to a concentration of 1% by volume and sterilized at 121°C for 20 minutes.

2) Added Courtside 5P68, Slaoff 6
Experiments were conducted to determine the inhibitory effect on mold and bacteria using S-2 medium containing S-20 and as a control, respectively, with the cap open and with the cap closed.

3) After 10 days, put caps on the ones that had been left open and see if the growth of mold and bacteria can be suppressed.
I checked R.

(The reason for capping the medium is that water evaporation of the medium is significant and it is difficult to maintain the shape of the medium if left open for more than 10 days.) Results 1) S-2 medium supplemented with Courtside 5P68 and Suraoff 620 was capped. When the cells were left open for one week, no mold, bacteria, etc. were observed in the culture medium at any concentration. However, mold grew on the additive-free S-2 medium 4 days after the cap was opened. There is no mold or bacteria on the ones with caps. Details are in the table.
9 shown in 2 2) 10 days after the cap is opened.

No mold or bacteria were generated in the culture medium tested with the cap attached. From the above experiments, court side 5P68,
It was confirmed that Suraoff 620 can be expected to have antifungal and antibacterial effects.

Table 2 Evaluation method: Visually 1 to 4 weeks+: Mold and bacteria generated: Heat generated by mold and bacteria Example method Courtside 5P68 and Suraoff 620 were added in the method shown in the experiment section, and heated to 121°C. After sterilization for 20 minutes, add Coleus and African S to the medium at each concentration.
I planted undew. S-Z without anti-mold and anti-bacterial treatment
Coleus and African Sundew were also planted in the medium and the growth of the plants was compared.

Results 1) Sujioff 620 concentration 0.01v as shown in Table-3
The medium to which O1% was added was contaminated with various bacteria.
ation) was not observed, and the growth of both plants was good without much difference compared to the S-2 medium. Furthermore, the medium remained solid and did not become water-soluble like Courtside 5P68 and S-2 medium.

2) On the other hand, at a concentration of 0.1 vol. 1% for both Coalside 5P68 and Slaoff 620, rooting of both plants was not observed, and at a concentration of 1 vol. 1%, Coleus completely withered.

3) No bacterial contamination was observed in the medium to which Courtside 5P68 and Suraoff 620 were added.

(Margin below) Table-3 Evaluation method 1)+ 2)+ Δ naked eye Occurrence of mold and bacteria Fever caused by mold and bacteria Medium condition: liquid Condition of medium: Solid Rooted No rooting

Claims (1)

[Scope of Claims] 1. (a) Provided with a lid that prevents microorganisms from entering the container and allows oxygen gas and carbon dioxide gas related to the metabolic physiology of plants to freely enter and exit the container, and at least plants A container that transmits the light necessary for the plant to survive and allows the inside to be observed; (b) a fungicide-containing medium stored at the bottom of the container; and (c) an ornamental plant transplanted into the medium. Characteristic ornamental plants in containers.