JPH0441594B2 - - Google Patents
Info
- Publication number
- JPH0441594B2 JPH0441594B2 JP26064788A JP26064788A JPH0441594B2 JP H0441594 B2 JPH0441594 B2 JP H0441594B2 JP 26064788 A JP26064788 A JP 26064788A JP 26064788 A JP26064788 A JP 26064788A JP H0441594 B2 JPH0441594 B2 JP H0441594B2
- Authority
- JP
- Japan
- Prior art keywords
- ethanolamines
- triethanolamine
- bacterium
- negative
- growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 150000002169 ethanolamines Chemical class 0.000 claims description 19
- 241000589580 Flavobacterium aquatile Species 0.000 claims description 7
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 10
- 238000000034 method Methods 0.000 description 8
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 238000000354 decomposition reaction Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000002173 cutting fluid Substances 0.000 description 2
- 239000010730 cutting oil Substances 0.000 description 2
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- KUWPCJHYPSUOFW-YBXAARCKSA-N 2-nitrophenyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-YBXAARCKSA-N 0.000 description 1
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 102100035882 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000006114 decarboxylation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- XHCADAYNFIFUHF-TVKJYDDYSA-N esculin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=C1)O)=CC2=C1OC(=O)C=C2 XHCADAYNFIFUHF-TVKJYDDYSA-N 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 150000002505 iron Chemical class 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000005486 sulfidation Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Description
［産業上の利用分野］
本発明はエタノールアミン類を分解するフラボ
バクテリウム・アクアタイルに関する。
エタノールアミン類は防錆剤として切削油剤に
利用される。切削油剤中におけるエタノールアミ
ン類の含有量は様々であるが、40％に達する場合
もある。
［従来の技術、発明が解決しようとする課題］
切削油廃液を処理する方法として擬集法、活性
汚泥法、活性炭吸着法などが知られているが、こ
れらの方法では該廃液中のエタノールアミン類、
特にトリエタノールアミン、ジエタノールアミン
は離分解性であるため、除去することができな
い。
しかも、エタノールアミン類は生体系への安全
性が疑問視され、水溶性であるため、一旦自然環
境に出ると、拡散も大きく、環境汚染を起こし、
自然浄化は困難である。そのため、未処理のまま
廃棄することには重大な問題がある。
［課題を解決するための手段］
そこで、本発明者らは微生物を利用してエタノ
ールアミン類を分解すべく、微生物の検索を行つ
たところ、横浜市金沢区内の土壌から分離した微
生物がトリエタノールアミンとジエタノールアミ
ンを効率よく分解することを見出し、本発明に到
達した。
すなわち本発明は、エタノールアミン類を分解
するフラボバクテリウム・アクアタイル
（Flavobacterium aquatile）に関する。
本菌は、上記した如く、横浜市金沢区内の土壌
からトリエタノールアミンを単独炭素源とする分
離培地を用いて単離されたものであり、以下のよ
うな菌学的性質を有している。
Ａ 形態学的性質
(1) 細胞の形および大きさ：桿菌 0.6×2.0μ
(2) 細胞の多形性：なし
(3) 運動性：なし
(4) 胞子：形成しない
(5) グラム染色性：陰性
(6) 抗酸性：なし
Ｂ 各培地における生育状態
(1) 標準寒天培地（30℃、５日間）
生育は良好、形状は円形、表面隆起は扁平
上、表面は円滑、色調は黄色
(2) トリプトソイ斜面培地
生育は適度、表面は円滑、色調は淡黄色
(3) トリエタノールアミン寒天培地（トリエタ
ノールアミン１％含有）
生育は適度、形状は円形、表面隆起は扁平
状、表面は円滑、色調は淡黄色
(4) ハートインフユージヨン寒天培地
生育は良好、形状は円形、色調は黄色
Ｃ 生理学的性質
(1) 硝酸塩の還元：還元する
(2) インドールの生成：陰性
(3) 硫化水素の生成：陰性
(4) デンプンの加水分解：陽性
(5) クエン酸の利用：クリステンゼン培地、シ
モンズ培地では利用しない
(6) リジン脱炭酸：陰性
(7) エスクリンの加水分解：陰性
(8) カゼインの加水分解：陽性
(9) 尿素分解：陰性
(10) カタラーゼ：陽性
(11) オキシラーゼ：陽性
(12) OF試験：酸化型
(13) 色素の生成：非水溶性黄色色素を生成
(14) 生育の範囲：温度15〜30℃
(15) 酸素要求性：好気性
(16) 塩化ナトリウム要求性：陰性
(17) β−ガラクトシダーゼ（ONPG）：陰性
(18) 糖類から酸の生成
[Industrial Application Field] The present invention relates to Flavobacterium aquatile which decomposes ethanolamines. Ethanolamines are used in cutting fluids as rust preventive agents. The content of ethanolamines in cutting fluids varies, but can reach up to 40%. [Prior Art and Problems to be Solved by the Invention] Known methods for treating cutting oil waste include the aggregation method, activated sludge method, and activated carbon adsorption method. kind,
In particular, triethanolamine and diethanolamine cannot be removed because they are separable. Moreover, the safety of ethanolamines to biological systems is questionable, and since they are water-soluble, once they are released into the natural environment, they diffuse widely and cause environmental pollution.
Natural purification is difficult. Therefore, there is a serious problem in disposing of it untreated. [Means for Solving the Problems] Therefore, the present inventors conducted a search for microorganisms in order to decompose ethanolamines using microorganisms, and found that microorganisms isolated from soil in Kanazawa Ward, Yokohama City were found to be trivial. The present invention was achieved by discovering that ethanolamine and diethanolamine can be efficiently decomposed. That is, the present invention relates to Flavobacterium aquatile that decomposes ethanolamines. As mentioned above, this bacterium was isolated from soil in Kanazawa Ward, Yokohama City using an isolation medium containing triethanolamine as the sole carbon source, and has the following mycological properties. There is. A Morphological properties (1) Cell shape and size: Bacillus 0.6×2.0μ (2) Cell pleomorphism: None (3) Motility: None (4) Spores: Not formed (5) Gram staining : Negative (6) Anti-acidity: None B Growth status in each medium (1) Standard agar medium (30℃, 5 days) Good growth, circular shape, flat surface ridges, smooth surface, yellow color ( 2) Trypto soy slant medium Growth is moderate, surface is smooth, color is pale yellow (3) Triethanolamine agar medium (contains 1% triethanolamine) Growth is moderate, shape is circular, surface ridges are flat, surface is smooth , pale yellow in color (4) Heart infusion agar medium Good growth, circular in shape, yellow in color C Physiological properties (1) Reduction of nitrate: Reducing (2) Formation of indole: Negative (3) Sulfidation Hydrogen generation: Negative (4) Starch hydrolysis: Positive (5) Citric acid usage: Not used in Christensen medium or Simmons medium (6) Lysine decarboxylation: Negative (7) Aesculin hydrolysis: Negative (8 ) Casein hydrolysis: Positive (9) Urea decomposition: Negative (10) Catalase: Positive (11) Oxirase: Positive (12) OF test: Oxidized type (13) Pigment production: Produces water-insoluble yellow pigment (14 ) Growth range: Temperature 15-30℃ (15) Oxygen requirement: Aerobic (16) Sodium chloride requirement: Negative (17) β-galactosidase (ONPG): Negative (18) Production of acid from sugars
【表】
以上の諸性質をバージーズ・マニユアル・オ
ブ・システマテイツク・バクテリオロジー、第１
版（1984年）に基づいて検索したところ、本菌は
フラボバクテリウム・アクアタイルであると確認
された。しかし、既知の菌株にはエタノールアミ
ン類を分解する能力を有するものは知られていな
い。
本菌はフラボバクテリウム・アクアタイルKK
−111と命名され、工業技術院微生物工業技術研
究所にFERM Ｐ−10335として寄託されている。
本発明においては、本菌を自然にもしくは人工的
手段によつて変異させて得られる変異株であつて
もエタノールアミン類を分解する能力を有するも
のはすべて包含される。
次に、本菌によるエタノールアミン類の分解方
法としては、本菌をエタノールアミン類と接触さ
せればよく、特別な条件を必要としないが、通常
はエタノールアミン類のほかに硫酸アンモニウ
ム、リン酸カリウム、マグネシウム塩、鉄塩など
の窒素源、無機塩類などを含む系で接触させる。
培養は好気的条件下、PH6.0〜8.0、温度15〜35
℃、好ましくは25〜30℃でエタノールアミン類が
十分に資化されるまで行う。エタノールアミン類
の初濃度は0.01〜３％、好ましくは0.05〜１％と
すべきであり、このような条件下では５〜10日程
度の培養によつてエタノールアミン類を完全に分
解することができる。
なお、本菌によるエタノールアミン類、特にト
リエタノールアミンおよびジエタノールアミンの
分解の評価法として、エタノールアミン類を単独
炭素源とし、硫酸アンモニウム、リン酸二水素カ
リウム、硫酸マグネシウムおよび塩化第二鉄を含
む培地（PH6.0〜8.0）に本菌を接種し、25〜30℃
で振とう培養を行い、本菌の増殖状態、エタノー
ルアミン類の濃度および溶存総有機炭素量
（TOC）を経時的に測定する方法がある。トリエ
タノールアミンおよびジエタノールアミンについ
ては、初濃度を１％以下とすれば、比較的短期間
に効率よく分解することができる。
［実施例］
次に、本発明を実施例によつて詳しく説明す
る。
実施例 １
200ml容フラスコにトリエタノールアミン0.1
％、硫酸アンモニウム0.1％、リン酸二水素カリ
ウム0.2％、硫酸マグネシウム40ppmおよび塩化
第二鉄40ppmを含む培地（PH7.0）100mlを入れ、
常法により滅菌後、フラボバクテリウム・アクア
タイルKK−111（FERM Ｐ−10335）を植菌し、
30℃で振とう培養を行い、その増殖状態、トリエ
タノールアミンおよび溶存TOCの残存量を経時
的に測定した。結果を第１図に示す。
図から明らかなように、本菌の増殖に伴なつて
トリエタノールアミンと溶存TOCの減少が認め
られ、培養９日目にはトリエタノールアミンの分
解率は99.9％以上となり、また溶存TOCの除去
率も92％以上であつた。
実施例 ２
実施例１において、トリエタノールアミンの代
りにジエタノールアミンを用いたこと以外は実施
例１と同様に行つた。その結果、培養６日目でジ
エタノールアミンの分解率は95％であつた。
［発明の効果］
本発明の微生物はエタノールアミン類を効率よ
く分解するので、該エタノールアミン類を含有す
る切削油廃液等を処理する場合に利用することが
できる。[Table] The above properties are summarized in Birdsey's Manual of Systematic Bacteriology, Volume 1.
(1984), this bacterium was confirmed to be Flavobacterium aquatile. However, none of the known bacterial strains is known to have the ability to degrade ethanolamines. This bacterium is Flavobacterium aquatile KK
-111, and has been deposited as FERM P-10335 at the Institute of Microbial Technology, Agency of Industrial Science and Technology.
The present invention includes all mutant strains that have the ability to decompose ethanolamines, even if they are mutant strains obtained by mutating this bacterium naturally or by artificial means. Next, the method for decomposing ethanolamines by this bacterium is to bring the bacterium into contact with ethanolamines, and no special conditions are required. , a nitrogen source such as magnesium salt, iron salt, and inorganic salts. Cultivation is carried out under aerobic conditions, pH 6.0-8.0, temperature 15-35.
C., preferably 25 to 30.degree. C., until the ethanolamines are fully assimilated. The initial concentration of ethanolamines should be 0.01 to 3%, preferably 0.05 to 1%, and under these conditions, ethanolamines can be completely decomposed by culturing for about 5 to 10 days. can. In addition, as a method for evaluating the decomposition of ethanolamines, particularly triethanolamine and diethanolamine, by this bacterium, we used a medium containing ethanolamines as the sole carbon source and containing ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, and ferric chloride ( Inoculate this bacteria at 25-30℃ (PH6.0-8.0).
There is a method in which the growth state of this bacterium, the concentration of ethanolamines, and the total amount of dissolved organic carbon (TOC) are measured over time by culturing with shaking. Triethanolamine and diethanolamine can be efficiently decomposed in a relatively short period of time if the initial concentration is 1% or less. [Example] Next, the present invention will be explained in detail with reference to an example. Example 1 Triethanolamine 0.1 in a 200ml flask
%, ammonium sulfate 0.1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 40ppm and ferric chloride 40ppm (PH7.0) 100ml.
After sterilization using a conventional method, Flavobacterium aquatile KK-111 (FERM P-10335) was inoculated.
A shaking culture was performed at 30°C, and the growth state, residual amount of triethanolamine and dissolved TOC were measured over time. The results are shown in Figure 1. As is clear from the figure, a decrease in triethanolamine and dissolved TOC was observed as the bacteria multiplied, and on the 9th day of culture, the decomposition rate of triethanolamine was over 99.9%, and the removal of dissolved TOC. The rate was also over 92%. Example 2 The same procedure as in Example 1 was carried out except that diethanolamine was used instead of triethanolamine. As a result, the decomposition rate of diethanolamine was 95% on the 6th day of culture. [Effects of the Invention] Since the microorganism of the present invention efficiently decomposes ethanolamines, it can be used to treat cutting oil waste fluid containing the ethanolamines.
第１図は、フラボバクテリウム・アクアタイル
KK−111の増殖状態とトリエタノールアミンお
よび溶存TOC濃度との関係を経時的に調べたグ
ラフである。
Figure 1 shows Flavobacterium aquatile
It is a graph showing the relationship between the proliferation state of KK-111 and the concentration of triethanolamine and dissolved TOC over time.
Claims (1)
リウム・アクアタイル。1 Flavobacterium aquatile decomposes ethanolamines.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26064788A JPH02109975A (en) | 1988-10-18 | 1988-10-18 | Novel bacterium to decompose ethanolamines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26064788A JPH02109975A (en) | 1988-10-18 | 1988-10-18 | Novel bacterium to decompose ethanolamines |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02109975A JPH02109975A (en) | 1990-04-23 |
| JPH0441594B2 true JPH0441594B2 (en) | 1992-07-08 |
Family
ID=17350821
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP26064788A Granted JPH02109975A (en) | 1988-10-18 | 1988-10-18 | Novel bacterium to decompose ethanolamines |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02109975A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63239103A (en) * | 1987-03-27 | 1988-10-05 | Ulvac Corp | Cubic boron nitride coated body and production thereof |
| US5686291A (en) * | 1995-10-04 | 1997-11-11 | Kabushiki Kaisha Neos | Corynebacterium SP. N having decomposability for ethanolamines |
-
1988
- 1988-10-18 JP JP26064788A patent/JPH02109975A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02109975A (en) | 1990-04-23 |
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