JPH0253482A - Bacterium having indole and skatole decomposing ability and microbiological decomposition of indole and skatole - Google Patents
Bacterium having indole and skatole decomposing ability and microbiological decomposition of indole and skatoleInfo
- Publication number
- JPH0253482A JPH0253482A JP63202860A JP20286088A JPH0253482A JP H0253482 A JPH0253482 A JP H0253482A JP 63202860 A JP63202860 A JP 63202860A JP 20286088 A JP20286088 A JP 20286088A JP H0253482 A JPH0253482 A JP H0253482A
- Authority
- JP
- Japan
- Prior art keywords
- skatole
- indole
- acinetobacter
- bacterium
- calcoacetecus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Chemical compound C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 title claims abstract description 66
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 title claims abstract description 64
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 title claims abstract description 33
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 229940074386 skatole Drugs 0.000 title claims abstract description 33
- 241000894006 Bacteria Species 0.000 title abstract description 7
- 238000000354 decomposition reaction Methods 0.000 title description 5
- 125000001041 indolyl group Chemical group 0.000 title description 2
- 230000002906 microbiologic effect Effects 0.000 title 1
- 241000589291 Acinetobacter Species 0.000 claims abstract description 29
- 244000005700 microbiome Species 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 11
- 235000015097 nutrients Nutrition 0.000 abstract description 10
- 210000003608 fece Anatomy 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 241000588624 Acinetobacter calcoaceticus Species 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 7
- 230000001877 deodorizing effect Effects 0.000 description 6
- 230000009965 odorless effect Effects 0.000 description 6
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 description 3
- 229940052299 calcium chloride dihydrate Drugs 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 3
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 3
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 3
- 229940099596 manganese sulfate Drugs 0.000 description 3
- 239000011702 manganese sulphate Substances 0.000 description 3
- 235000007079 manganese sulphate Nutrition 0.000 description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- DXXYTBCIXZGERI-UHFFFAOYSA-N O.O.O.O.O.O.O.[Fe] Chemical compound O.O.O.O.O.O.O.[Fe] DXXYTBCIXZGERI-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000004332 deodorization Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 210000001822 immobilized cell Anatomy 0.000 description 2
- 229910000358 iron sulfate Inorganic materials 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010022998 Irritability Diseases 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 241000187654 Nocardia Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241000605118 Thiobacillus Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 210000001179 synovial fluid Anatomy 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Indole Compounds (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、新規な微生物と、その微生物を使用した脱臭
方法に関する。さらに詳しくは、インドールおよびスカ
トール分解能を有する微生物並びにインドールおよびス
カトールの微生物的分解方法に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a novel microorganism and a deodorizing method using the microorganism. More specifically, the present invention relates to microorganisms capable of decomposing indole and skatole and a method for microbial decomposition of indole and skatole.
［従来の技術］
インドールおよびスカトールは特有の強い糞臭および不
快な臭気があり、悪臭の原因物質の一つである。[Prior Art] Indole and skatole have a unique strong fecal odor and an unpleasant odor, and are one of the substances that cause malodor.
従来、インドールおよびスカトールの脱臭方法として、
たとえば、インドールおよびスカトール分解能を有する
微生物、具体的には、ラクトバチルス属（Ｌａｃｔｏｂ
ａｃｉｌｌｕｓ） （特公昭５７−４９１９３号）、
チオバチルス属（Ｔｈｉｏｂａｃｉｌｌｕｓ） （特開
昭５５−４８３８６号）、アルカリ土類金属（＾Ｉｃａ
ｌ ｉｇｅｎｅｓ）（特開昭６１−４６２４０ ＠）
、セラチア属（Ｓｅｒｒａｔ １ａ）（特開昭６１−４
６２４０号）、シュードモナス属（ＰＳｅｕｄＯｍＯｎ
ａＳ） （特開昭５５−４８３８６ ＠）またはノカ
ルデア属（ＮＯＣａｒｄｉａ） ［ラントヴイルトシャ
フトリヒエ・フォルシュング・ゾンダーヘフト（Ｌａｎ
ｄｗｉ ｒｔｓｃｈ、 Ｆｏｒｓｃｈ、 ５ｏｎｄｅｒ
ｈ、 ）第３７巻、第５４１〜５５０頁、１９８０年］
などを使用した脱臭方法が知られている。Conventionally, as a method for deodorizing indole and skatole,
For example, microorganisms that have the ability to degrade indole and skatole, specifically Lactobacillus spp.
acillus) (Special Publication No. 57-49193),
Thiobacillus (Japanese Unexamined Patent Publication No. 55-48386), alkaline earth metals (^Ica
l igenes) (JP-A-61-46240 @)
, Serratia 1a (JP-A-61-4
6240), Pseudomonas sp.
aS) (JP 55-48386 @) or Nocardia genus [Landwirtschaftlichie Forschung Sonderheft (Lan
dwi rtsch, Forsch, 5onder
h, ) Vol. 37, pp. 541-550, 1980]
Deodorizing methods using, etc., are known.
［発明が解決しようとする課題］
前述の如く、微生物を使用する脱臭方法は、いくつか知
られており、経済性、効果などの点で優れた方法でおる
が、アシネトバクタ−属（Ａｃｉｎｅｔｏｂａｃｔｅｒ
）を使用した脱臭方法に関シテハ知られていない。また
、概して、インドールおよびスカトールの脱臭に関して
は、十分な効果を発揮しているとは言えないのが現状で
ある。[Problems to be Solved by the Invention] As mentioned above, there are several known deodorizing methods that use microorganisms, and these methods are excellent in terms of economy and effectiveness.
) is not known about the deodorizing method. Furthermore, in general, it cannot be said that the deodorization of indole and skatole is sufficiently effective.
［課題を解決するための手段］
本発明者らは、微生物を用いてインドールおよびスカト
ールを分解する方法に関して鋭意研究を重ねた結果、ア
シネトバクタ−属（Ａｃｉｎｅｔｏｂａｃｔｅｒ）に属
する微生物がインドールおよびスカトールを効率よく分
解し、インドールおよびスカトールの脱臭に有用である
ことを見出し、本発明を完成した。[Means for Solving the Problems] As a result of extensive research into methods for degrading indole and skatole using microorganisms, the present inventors found that microorganisms belonging to the genus Acinetobacter can efficiently degrade indole and skatole. The present invention was completed based on the discovery that it is useful for deodorizing indole and skatole.
本発明の目的は、新規な微生物と、その微生物を使用し
た優れた脱臭方法を提供することにおる。An object of the present invention is to provide a novel microorganism and an excellent deodorization method using the microorganism.
本発明のアシネトバクタ−属（Ａｃｉｎｅｔｏｂａｃｔ
ｅｒ）に属する微生物の具体例とその菌学的性質を表１
に示す。The Acinetobacter genus (Acinetobacter) of the present invention
Table 1 shows specific examples of microorganisms belonging to er) and their mycological properties.
Shown below.
表−１（続き）
以上の諸性状をバーシーズ・マニュアル・オブ・ディタ
ミネイティブ・バタテリオロジー（Ｂｅｒｇｅｙ’ｓ
Ｍａｎｕａｌ ｏｆ ＤｅｔｅｒｍｉｎａｔｉｖｅＢａ
ｃｔｅｒｉｏｌｏｇｙ）第８版（１９７４年）に照して
検討したところ、本菌株の性状がアシネトバクター・カ
ルコアセテカス（ＡＣｉｎｅｔＯｂａＣｔｅｒ Ｃａ１
ＣＯａＣｅｔｉＣＬＩＳ）の性状に酷似することから、
本菌株は、アシネトバクター・カルコアセテカス（Ａｃ
＋ ｎｅｔｏｂａｃｔｅｒｃａｔｃｏａｃｅｔｉｃｕ
ｓ）に属する菌として同定し、本菌株をそれぞれアシネ
トバクター・カルコアセテカス（Ａｃｉｎｅｔｏｂａｃ
ｔｅｒ ｃａｌｃｏａｃｅｔｉｃｕｓ） Ｉ Ｄ ６
（微工研菌寄第１００８５号）、ｌ［）１１２（微工研
菌寄第１００８６号）およびＪ［）４２１（微工研菌奇
第１００８７号）と命名した。Table 1 (Continued) The above properties are summarized in Bergey's Manual of Determinative Batteriology (Bergey's Manual of Determinative Batteriology).
Manual of DeterminativeBa
cteriology) 8th edition (1974), the characteristics of this strain were found to be that of Acinetobacter calcoacetecus (ACinetobacter calcoacetecus).
Because the properties are very similar to those of COaCetiCLIS),
This strain is Acinetobacter calcoacetecus (Ac
+ netobactercatcoaceticu
These strains were identified as belonging to Acinetobacter s), respectively.
ter calcoaceticus) I D 6
(Feikoku Kenkyoku No. 10085), l[)112 (Feikoku Kenkyoku No. 10086), and J[)421 (Feikoku Kenkyoku No. 10087).
なお、本菌株は、動植物に対する病原性はない。Note that this strain is not pathogenic to animals and plants.
本発明において、前記菌株のほかその自然変異菌株およ
び人工変異菌株は勿論のこと、アシネトバクタ−属（Ａ
ｃ １ｎｅｔｏｂａｃｔｅｒ）に属し、インドールおよ
びスカトール分解能を有する菌ならすべて使用すること
ができる。In the present invention, in addition to the above-mentioned strains, as well as natural and artificial mutant strains thereof, the genus Acinetobacter (A
Any bacterium that belongs to the genus C. 1 netobacter and has the ability to degrade indole and skatole can be used.
つぎに、本発明の新規なアシネトバクタ−属（Ａｃ １
ｎｅｔＯｂａｃｔｅｒ）に属する微生物の培養法を説明
する。Next, the novel Acinetobacter genus (Ac 1
A method for culturing microorganisms belonging to the genus netObacter will be explained.
本発明のアシネトバクタ−属（Ａｃ １ｎｅｔｏｂａｃ
ｔｅｒ）に属する微生物は、通常知られている微生物の
公知の培養法、たとえば、有機栄養源および無機栄養源
を含有する水性栄養培地中で好気性条件下に培養するこ
とができる。The Acinetobacter genus (Ac 1netobacter) of the present invention
Microorganisms belonging to the group ter) can be cultured according to commonly known culture methods for microorganisms, for example, under aerobic conditions in an aqueous nutrient medium containing organic and inorganic nutrients.
そのような培地は、醗酵技術において通常使用される多
数の栄養培地のいずれであってもよく、たとえば、肉汁
、酵母エキス、ペプトン、牛脳および心臓抽出物などの
有機栄養源；ナトリウム、カリウム、マグネシウム、カ
ルシウム、マンガン、亜鉛、鉄、塩素、炭酸、硫酸、硝
酸、リン酸などのイオンを生成しうる可溶性塩からなる
無機栄養源などを適宜含有する培地が好ましい。更に必
要に応じて微生物の生育に必要な各種の有機栄養源、無
機栄養源などを培地に添加することができる。Such a medium may be any of a number of nutrient media commonly used in fermentation technology, including organic nutrients such as meat broth, yeast extract, peptone, bovine brain and heart extract; sodium, potassium, A medium containing an appropriate inorganic nutrient source consisting of a soluble salt capable of producing ions such as magnesium, calcium, manganese, zinc, iron, chlorine, carbonic acid, sulfuric acid, nitric acid, and phosphoric acid is preferred. Furthermore, various organic nutrient sources, inorganic nutrient sources, etc. necessary for the growth of microorganisms can be added to the medium as necessary.
本菌株は、前記のような液体培地に直接菌体を接種して
培養すればよい。The present bacterial strain may be cultured by directly inoculating the bacterial cells into a liquid medium as described above.
培養温度は、微生物が発育しうる範囲内で適宜設定され
るが、通常２０〜４５°Ｃ１好ましくは２５〜３８℃で
ある。The culture temperature is appropriately set within a range in which microorganisms can grow, and is usually 20 to 45°C, preferably 25 to 38°C.
また、培地のｐＨは、通常ａｔ（５〜８、好ましくは１
）Ｈ６〜７，５で調整される。In addition, the pH of the medium is usually at (5 to 8, preferably 1
) Adjusted in H6-7,5.
培養期間は、使用する培地の種類により異なるが、通常
１〜２日程度である。The culture period varies depending on the type of medium used, but is usually about 1 to 2 days.
本発明のアシネトバクタ−属（八Ｃ１ｎｅｔＯｂａＣ１
ｅｒ’）に属する微生物は、たとえば、インドールおよ
びスカトールを、予め培地に添加接触させた場合、また
は培養途中でインドールおよびスカトールを、培地に添
加接触させた場合、いずれの場合にも、インドールおよ
びスカトールを効果的に分解させる。The Acinetobacter genus (8C1netObaC1) of the present invention
For example, microorganisms belonging to the microorganisms belonging to the group er') can be exposed to indole and skatole in cases where indole and skatole are added to the culture medium in advance or when indole and skatole are added to the culture medium during culture. effectively decompose.
このように、本発明は、たとえば、インドールおよびス
カトール分解能を有するアシネトバクタ−属（八ｃｉｎ
ｅｔｏｂａｃｔｅｒ）に属する微生物の培養液または該
培養液より遠心ン濾過などの通常の分離方法で採集した
菌体あるいはそれらの固定化菌体を、糞便を含有する廃
液またはインドールおよびスカトールを含有する大気な
どに接触させることにより、インドールおよびスカトー
ルを効果的に分解させることができる。Thus, the present invention provides, for example, Acinetobacter (octocin) having indole and skatole degrading ability
A culture solution of microorganisms belonging to the genus Etobacter, or cells collected from the culture solution by a conventional separation method such as centrifugation or filtration, or their immobilized cells are added to waste liquid containing feces or air containing indole and skatole, etc. Indole and skatole can be effectively decomposed by contacting with.
［発明の効果］
本発明のアシネトバクタ−属（八Ｃ１ｎｅｔＯＩｌ）ａ
ｃｔｅｌ’）に属する菌体またはそれらの固定化菌体は
、たとえば、産業用または家庭用の脱臭剤として利用す
ることができる。[Effect of the invention] Acinetobacter genus (8C1netOIl)a of the present invention
Ctel') or their immobilized cells can be used, for example, as an industrial or household deodorizer.
［実施例］ つぎに実施例によって本発明を具体的に説明する。[Example] Next, the present invention will be specifically explained with reference to Examples.
なお、実施例中、パーセントはすべて重量パーセントで
ある。In addition, all percentages in the examples are weight percentages.
実施例１
０．１％酵母エキス、０．３％リン酸水索二カリウム、
０．０２％硫酸マグネシウム・７水塩、０．０００５％
塩化カルシウム・２水塩、０．００７％硫酸マンガン・
４〜６水塩、０．００１％硫酸亜鉛・７水塩およびｏ、
ｏｏｉ％硫酸鉄・７水塩からなる液体培地１０７！に
インドール５０ｔｓ／ｍｌを加え、ついでアシネトバク
ター・カルコアセテカス（Ａｃ ｉ ｎｅｔｏｂａｃｔ
ｅｒｃａｌｃｏａｃｅｔｉｃｕｓ） Ｉ Ｄ ６を接種
し、２７°Ｃ，１）８７．２で一夜静置して、前培養液
とした。この前培養液を、新鮮な前記と同じ成分からな
る液体培地２５０威に接種し、２７°Ｃ，ｐｌ（７，２
で６時間振盪培養した。こうして得られた本培養液を採
取し、３０００ｐｐｍで１０分間遠心濾過して、得られ
た上滑液１．５戒にファン・ウルツ（Ｖａｎ Ｕｒｋ
）試薬１．５ｄを加え、１０分後に５６５ｎｍで比色定
量した結果、インドールは検出されず、無臭であった。Example 1 0.1% yeast extract, 0.3% dipotassium phosphate,
0.02% magnesium sulfate heptahydrate, 0.0005%
Calcium chloride dihydrate, 0.007% manganese sulfate.
4-6 hydrate, 0.001% zinc sulfate heptahydrate and o,
Liquid medium 107 consisting of ooi% iron sulfate and heptahydrate! 50ts/ml of indole was added to the solution, and then Acinetobacter calcoacetecus (Acinetobacter calcoacetecus) was added.
ercalcoaceticus) ID 6 was inoculated and allowed to stand overnight at 27°C, 1) 87.2°C to prepare a preculture solution. This pre-culture solution was inoculated into 250 volumes of a fresh liquid medium consisting of the same components as above, heated at 27°C, pl (7,2
The cells were cultured with shaking for 6 hours. The main culture fluid obtained in this way was collected and centrifugally filtered at 3000 ppm for 10 minutes, and 1.5 liters of the obtained synovial fluid was added to Van Urk.
) Addition of 1.5 d of reagent and colorimetric determination at 565 nm after 10 minutes revealed that indole was not detected and was odorless.
実施例２
アシネトバクター・カルコアセテカス
（Ａｃｉｎｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉ
ｃｕｓ） Ｉ Ｄ １１２を用い、実施例１と同様に
してインドール分解能を比色定量した結果、培養後６時
間でインドールは検出されず、無臭であった。Example 2 Acinetobacter calcoaceti
As a result of colorimetric determination of indole decomposition ability in the same manner as in Example 1 using C.cus) ID 112, indole was not detected 6 hours after culturing and was odorless.
実施例３
アシネトバクター・カルコアセテカス
（Ａｃｉｎｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉ
ｃｕｓ） ｌ ［） ４２１を用い、実施例１と同様
にしてインドール分解能を比色定量した結果、培養後６
時間でインドールは検出されず、無臭であった。Example 3 Acinetobacter calcoaceti
As a result of colorimetric determination of indole decomposition ability in the same manner as in Example 1 using
No indole was detected and was odorless.
実施例４
０．１％酵母エキス、０．３％リン酸水素二カリウム、
０．０２％硫酸マグネシウム・７水塩、０．０００５％
塩化カルシウム・２水塩、０．００７％硫酸マンガン・
４〜６水塩、０．００１％硫酸亜鉛・γ水塩および０、
００１％硫酸鉄・７水塩からなる液体培地１０ｍ１にス
カトール５０ｔｔ３／ｍ（ｌを加え、ついでアシネトバ
クター・カルコアセテカス（ＡＣｉｒｌｅｔＯｂａＣｔ
ｅｒｃａｌｃｏａｃｅｔｉｃｕｓ） Ｉ Ｄ １１２を
接種し、２７°Ｃ，ｐＨ７，２で一夜静置して、前培養
液とした。この前培養液を、新鮮な前記と同じ成分から
なる液体培地２５０ｄに接種し、２７°Ｃ，ＤＨ７，２
で８時間振盪培養した。Example 4 0.1% yeast extract, 0.3% dipotassium hydrogen phosphate,
0.02% magnesium sulfate heptahydrate, 0.0005%
Calcium chloride dihydrate, 0.007% manganese sulfate.
4-6 hydrate salt, 0.001% zinc sulfate/gamma hydrate salt and 0,
50 tt3/ml (l) of skatole was added to 10 ml of a liquid medium consisting of 001% iron sulfate/heptahydrate, and then Acinetobacter calcoacetecus (ACirletObaCt
ercalcoaceticus) ID 112 and left standing overnight at 27°C and pH 7.2 to prepare a preculture solution. This pre-culture solution was inoculated into 250d of fresh liquid medium consisting of the same ingredients as above, and the temperature was 27°C, DH7, 2.
The cells were cultured with shaking for 8 hours.
こうして得られた本培養液を採取し、３０００ｐｐｍで
１０分間遠心濾過して、得られた上清液１．５ ｒＩＩ
ｌにファン・ウルツ（Ｖａｎ Ｕｒｋ）試薬１．５ｄを
加え、１０分後に５７８ｎｍで比色定量した結果、スカ
トールは検出されず、無臭であった。The main culture solution thus obtained was collected and centrifugally filtered at 3000 ppm for 10 minutes, resulting in a supernatant solution of 1.5 rII
1.5 d of Van Urk reagent was added to the solution, and after 10 minutes, colorimetric determination at 578 nm revealed that skatole was not detected and was odorless.
実施例５
０．１％酵母エキス、０．３％リン酸水素二カリウム、
０．１％リン酸水素アンモニウム、０．１％塩化ナトリ
ウム、０．０２％硫酸マグネシウム・７水塩、０、００
０５％塩化カルシウム・２水塩ζ０．００７％硫酸マン
ガン・４〜６水塩、０．００１％硫酸亜鉛・７水塩およ
び０．００１％硫酸鉄・７水塩からなる液体培地１０ｍ
１にスカトール５０ｉ／ｍｌを加え、ついでアシネトバ
クター・カルコアセテカス（Ａｃｉｎｅｔｏｂａｃｔｅ
ｒ ｃａｌｃｏａｃｅｔｉｃｕｓ） Ｉ Ｄ６を接種
し、２７℃、ｔ）８７．２で一夜静置し、培養した。こ
うして得られた本培養液ｉ、ｓ ｍｌにファン・ウルツ
（ＶａｎＵｒｋ）試薬１．５ ｍｌを加え、１０分後に
５７８ ｎｍで比色定置した結果、スカトールは検出さ
れず、無臭であった。Example 5 0.1% yeast extract, 0.3% dipotassium hydrogen phosphate,
0.1% ammonium hydrogen phosphate, 0.1% sodium chloride, 0.02% magnesium sulfate heptahydrate, 0,00
10 m of liquid medium consisting of 0.05% calcium chloride dihydrate ζ 0.007% manganese sulfate tetra-hexahydrate, 0.001% zinc sulfate heptahydrate and 0.001% iron sulfate heptahydrate
50 i/ml of skatole was added to 1, and then Acinetobacter calcoacetecus (Acinetobacter calcoacetecus) was added.
r calcoaceticus) I D6 was inoculated and cultured at 27°C and t)87.2 overnight. 1.5 ml of VanUrk reagent was added to the main culture solution i, s ml thus obtained, and 10 minutes later, colorimetry was performed at 578 nm. As a result, skatole was not detected and it was odorless.
実施例６
アシネトバクター・カルコアセテカス
（Ａｃｉｎｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉ
ｃｕｓ） Ｉ Ｄ ４２１を用い、実施例５と同様に
してスカトール分解能を比色定置した結果、−夜静置培
養後スカトールは検出されず、無臭であった。Example 6 Acinetobacter calcoaceti
As a result of colorimetric determination of skatole decomposition ability in the same manner as in Example 5 using ID 421, skatole was not detected after overnight culture and was odorless.
以 上Below Up
Claims (7)
ｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉｃｕｓ）Ｉ
Ｄ６およびその変異菌株。(1) Acinetobacter calcoacetecus (Acin)
etobacter calcoaceticus) I
D6 and its mutant strains.
ｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉｃｕｓ）Ｉ
Ｄ６（微工研菌寄第１００８５号）である特許請求の範
囲第（１）項記載の微生物。(2) Acinetobacter calcoacetecus (Acin)
etobacter calcoaceticus) I
The microorganism according to claim (1), which is D6 (Feikoken Bokuyori No. 10085).
ｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉｃｕｓ）Ｉ
Ｄ１１２およびその変異菌株。(3) Acinetobacter calcoacetecus (Acin)
etobacter calcoaceticus) I
D112 and its mutant strains.
ｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉｃｕｓ）Ｉ
Ｄ１１２（微工研菌寄第１００８６号）である特許請求
の範囲第（３）項記載の微生物。(4) Acinetobacter calcoacetecus (Acin)
etobacter calcoaceticus) I
The microorganism according to claim (3), which is D112 (KAIKEN BIYORI NO. 10086).
ｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉｃｕｓ）Ｉ
Ｄ４２１およびその変異菌株。(5) Acinetobacter calcoacetecus (Acin)
etobacter calcoaceticus) I
D421 and its mutant strains.
ｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉｃｕｓ）Ｉ
Ｄ４２１（微工研菌寄第１００８７号）である特許請求
の範囲第（５）項記載の微生物。(6) Acinetobacter calcoacetecus (Acin)
etobacter calcoaceticus) I
The microorganism according to claim (5), which is D421 (Feikoken Bibori No. 10087).
ネトバクター属（Ａｃｉｎｅｔｏｂａｃｔｅｒ）に属す
る微生物とインドールおよびスカトールを接触させるこ
とを特徴とするインドールおよびスカトールの分解方法
。(7) A method for decomposing indole and skatole, which comprises bringing indole and skatole into contact with a microorganism belonging to the genus Acinetobacter that has the ability to decompose indole and skatole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63202860A JP2719703B2 (en) | 1988-08-15 | 1988-08-15 | Microorganisms capable of degrading indole and skatole and methods for microbial degradation of indole and skatole |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63202860A JP2719703B2 (en) | 1988-08-15 | 1988-08-15 | Microorganisms capable of degrading indole and skatole and methods for microbial degradation of indole and skatole |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0253482A true JPH0253482A (en) | 1990-02-22 |
| JP2719703B2 JP2719703B2 (en) | 1998-02-25 |
Family
ID=16464393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63202860A Expired - Lifetime JP2719703B2 (en) | 1988-08-15 | 1988-08-15 | Microorganisms capable of degrading indole and skatole and methods for microbial degradation of indole and skatole |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2719703B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5557429A (en) * | 1992-10-20 | 1996-09-17 | Fuji Xerox Co., Ltd. | Image signal processing apparatus |
| WO2012066749A1 (en) | 2010-11-15 | 2012-05-24 | Toyota Jidosha Kabushiki Kaisha | Microorganism and deodorizer containing the same |
| CN109402008A (en) * | 2018-11-15 | 2019-03-01 | 中国农业科学院饲料研究所 | One plant of acinetobacter calcoaceticus TAT1-6A and its application with indoles degradation capability |
| CN113930360A (en) * | 2021-10-20 | 2022-01-14 | 大连理工大学 | Acinetobacter and method and application thereof for degrading indole |
-
1988
- 1988-08-15 JP JP63202860A patent/JP2719703B2/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5557429A (en) * | 1992-10-20 | 1996-09-17 | Fuji Xerox Co., Ltd. | Image signal processing apparatus |
| WO2012066749A1 (en) | 2010-11-15 | 2012-05-24 | Toyota Jidosha Kabushiki Kaisha | Microorganism and deodorizer containing the same |
| US8741625B2 (en) | 2010-11-15 | 2014-06-03 | Toyota Jidosha Kabushiki Kaisha | Microorganism and deodorizer containing the same |
| CN109402008A (en) * | 2018-11-15 | 2019-03-01 | 中国农业科学院饲料研究所 | One plant of acinetobacter calcoaceticus TAT1-6A and its application with indoles degradation capability |
| CN113930360A (en) * | 2021-10-20 | 2022-01-14 | 大连理工大学 | Acinetobacter and method and application thereof for degrading indole |
| CN113930360B (en) * | 2021-10-20 | 2023-12-01 | 大连理工大学 | Acinetobacter and method for degrading indole by using same and application of acinetobacter |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2719703B2 (en) | 1998-02-25 |
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