JP2562347B2 - Method for degrading thiodipropionic acid by microorganisms - Google Patents
Method for degrading thiodipropionic acid by microorganismsInfo
- Publication number
- JP2562347B2 JP2562347B2 JP8824288A JP8824288A JP2562347B2 JP 2562347 B2 JP2562347 B2 JP 2562347B2 JP 8824288 A JP8824288 A JP 8824288A JP 8824288 A JP8824288 A JP 8824288A JP 2562347 B2 JP2562347 B2 JP 2562347B2
- Authority
- JP
- Japan
- Prior art keywords
- thiodipropionic acid
- microorganisms
- genus
- degrading
- tdpa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【発明の詳細な説明】 本発明は微生物によるチオジプロピオン酸の分解方法
に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for degrading thiodipropionic acid by a microorganism.
チオジプロピオン酸はアクリロニトリルと硫化ソーダ
を反応させ、これを加水分解して工業的に合成され、プ
ラスチック関係の酸化防止剤の原料として広い用途を有
している。Thiodipropionic acid is industrially synthesized by reacting acrylonitrile with sodium sulfide and hydrolyzing this, and has a wide range of uses as a raw material for antioxidants related to plastics.
チオジプロピオン酸製造においては、その製造工程で
生ずる工業廃棄物中に、チオジプロピオン酸が含まれて
おり、新たな環境汚染源として憂慮されているところで
ある。しかも、チオジプロピオン酸は自然界には存在し
ない化合物であり、廃棄しても分解されることなく、い
つまでも残っているために、広範囲の微生物に対して安
定であると考えられてきた。In the production of thiodipropionic acid, thiodipropionic acid is contained in the industrial waste produced in the production process, and it is feared as a new source of environmental pollution. Moreover, thiodipropionic acid is a compound that does not exist in nature, and it has been considered stable against a wide range of microorganisms because it does not decompose even if it is discarded and remains forever.
本発明者らは、このように環境汚染源となるチオジプ
ロピオン酸を分解し、環境浄化に役立つ微生物が生存す
るのではないかとの想定のもとに、自然界よりチオジプ
ロピオン酸を分解する微生物のスクリーニングを行っ
た。The present inventors decomposed thiodipropionic acid, which is a source of environmental pollution, in this way, and based on the assumption that microorganisms useful for environmental purification may survive, microorganisms that decompose thiodipropionic acid from the natural world. Was screened.
その結果、表−１〜６に示す菌株が、唯一炭素源とし
てチオジプロピオン酸を含有する培地で生育し、チオジ
プロピオン酸を分解することが確認された。As a result, it was confirmed that the strains shown in Tables 1 to 6 grew in a medium containing thiodipropionic acid as the sole carbon source and decomposed thiodipropionic acid.
これら菌株の菌学的性質を示すと次の通りである。 The mycological properties of these strains are as follows.
以上の菌学的性質をバージーの細菌分類書〔Bergey's
Manual of Systmatic Bacteriology vol.1（1984）,vo
l.2（1986）およびBergey's Manual of Determinative
Bacteriology 8th Ed.（1974）〕に基づいて検索し、表
−１のＮ−8701菌部はミクロバクテリウム（Microbacte
rium）属、表−２のＮ−8700、Ｎ−8702およびＮ−8707
菌株はシュードモナス（Pseudomonas）属、表−３のＮ
−8703およびＮ−8708菌株はバチルス（Bacillus）属、
表−４のＮ−8704菌株はアルカリゲネス（Alcaligene
s）属、表−５のＮ−8705菌株はシトロバクター（Citro
bacter）属および表−６のＮ−8706菌株はフラボバクテ
リウム（Flavobacterium）属に属する細菌と同定され
た。 The above-mentioned mycological properties are based on the Burj's bacterial classification document [Bergey's
Manual of Systmatic Bacteriology vol.1 (1984), vo
l.2 (1986) and Bergey's Manual of Determinative
Bacteriology 8th Ed. (1974)], and the N-8701 bacterial part in Table 1 was identified as Microbacterium (Microbacte
Rium), N-8700, N-8702 and N-8707 of Table-2
Strains are Pseudomonas spp., N in Table-3
-8703 and N-8708 strains are of the genus Bacillus,
The N-8704 strains in Table 4 are Alcaligene (Alcaligene
s) genus, N-5870 strain of Table-5 is Citrobacterium (Citro
Bacterium) and the N-8706 strain of Table 6 were identified as bacteria belonging to the genus Flavobacterium.
そして、Ｎ−8701、Ｎ−8702、Ｎ−8703、Ｎ−8704、
Ｎ−8705よびＮ−8706菌株は、微生物工業技術研究所
に、それぞれミクロバクテリウムsp.N−8701〔微工研菌
寄第9949号（FERM P−9949）〕、シュードモナスsp.N−
8702〔微工研菌寄第9950号（FERM P−9950）〕、バチル
スsp.N−8703〔微工研菌寄第9951号（FERM P−995
1）〕、アルカリゲネスsp.N−8704〔微工研菌寄第9952
号（FERM P−9952）〕、シトロバクターsp.N−8705〔微
工研菌寄第9953号（FERM P−9953）〕およびフラボバク
テリウムsp.N−8706〔微工研菌寄第9954号（FERM P−99
54）〕として寄託されている。And N-8701, N-8702, N-8703, N-8704,
The N-8705 and N-8706 strains were obtained from the Institute of Microbial Science and Technology, respectively, at Microbacterium sp. N-8701 [Microtechnology Research Institute, Microbiology No. 9949 (FERM P-9949)], Pseudomonas sp.
8702 [Microtechnical Research Institute 9950 (FERM P-9950)], Bacillus sp. N-8703 [Microtechnical Research Institute 9991 (FERM P-995)
1)], Alcaligenes sp. N-8704 [Microtechnology Research Institute
No. (FERM P-9952)], Citrobacter sp. N-8705 (Microtechnological Research Institute No. 9953 (FERM P-9953)) and Flavobacterium sp. N-8706 (Microtechnical Research Institute No. 9954). (FERM P-99
54)] has been deposited.
従って、本発明は、ミクロバクテリウム（Microbacte
rium）属、シュードモナス（Pseudomonas）属、バチル
ス（Bacillus）属、アルカリゲネス（Alcaligenes）
属、シトロバクター（Citrobacter）属またはフラボバ
クテリウム（Flavobacterium）属に属し、チオジプロピ
オン酸を分解する能力を有する微生物の中から選ばれた
一種または二種以上の微生物を培養液、分離生菌体、も
しくはこれらの処理物とチオジプロピオン酸とを好気的
に接触せしめて、チオジプロピオン酸を分解することを
特徴とする微生物によるチオジプロピオン酸の分解方法
である。Therefore, the present invention provides a Microbacte
rium), Pseudomonas, Bacillus, Alcaligenes
A culture broth or a viable isolate of one or more microorganisms belonging to the genus, Citrobacter genus or Flavobacterium genus and having the ability to decompose thiodipropionic acid A method for decomposing thiodipropionic acid by a microorganism, which comprises decomposing thiodipropionic acid by aerobically contacting the body or a treated product thereof with thiodipropionic acid.
本発明において分解の対象となるのは、チオジプロピ
オン酸（以下、TDPAと略す）であるが、この酸は、アル
カリ金属塩、アンモニウム塩、有機アミン塩等の塩の形
になっているものでもよい。In the present invention, the target of decomposition is thiodipropionic acid (hereinafter abbreviated as TDPA), but this acid is in the form of a salt such as an alkali metal salt, an ammonium salt, or an organic amine salt. But it's okay.
本発明で使用する培地は、これらの菌が属する細菌の
培養に用いることができる培地であればよく、格別の制
限はない。また、本発明の微生物はそれぞれ単独使用あ
るいは混合使用のいずれであってもよい。The medium used in the present invention is not particularly limited as long as it can be used for culturing bacteria to which these bacteria belong. Further, the microorganisms of the present invention may be used individually or as a mixture.
培地の炭素源として用いられるものは、菌が資化し得
るものであれば、いかなるものでもよく、例えば、グル
コース、シュクロース、澱粉などが用いられる。また、
窒素源として用いられるものは、菌が資化し得るもので
あれば、いかなるものでもよく、例えば、リン酸アンモ
ニウム、硫酸アンモニウム、酵母エキス、肉エキスなど
が用いられる。さらに必要に応じて、無機塩類としてリ
ン酸一カリウム、リン酸二ナトリウム、硫酸マグネシウ
ム、硫酸第一鉄、塩化カルシウム、硫酸亜鉛、硫酸マン
ガンなど、微量栄養物質としてビタミン、アミノ酸、核
酸なども適宜添加される。また、必要に応じて界面活性
剤や消泡剤を添加してもよい。What is used as a carbon source of the medium may be any as long as it can be assimilated by the bacterium, and for example, glucose, sucrose, starch and the like are used. Also,
Any nitrogen source may be used as long as it can be assimilated by the bacterium, and examples thereof include ammonium phosphate, ammonium sulfate, yeast extract, and meat extract. In addition, if necessary, inorganic potassium salts such as monopotassium phosphate, disodium phosphate, magnesium sulfate, ferrous sulfate, calcium chloride, zinc sulfate, and manganese sulfate are added as vitamins, amino acids, nucleic acids, etc. as trace nutrients. To be done. Moreover, you may add a surfactant and a defoaming agent as needed.
培養温度は通常15〜50℃の範囲、pHは通常５〜11の範
囲、培養時間は通常１〜30日の範囲であるが、各種微生
物について高い該分解活性が得られるよう、各種条件を
設定するのが好ましい。The culturing temperature is usually in the range of 15 to 50 ° C., the pH is usually in the range of 5 to 11, and the culturing time is usually in the range of 1 to 30 days, but various conditions are set so that the high decomposition activity of various microorganisms can be obtained. Preferably.
TDPAの分解は、TDPAを、培養中の培養液に存在させる
か、または培養液の培養物、分離生菌体、もしくはこれ
らの処理物と接触させることにより行われる。Degradation of TDPA is carried out by allowing TDPA to be present in the culture medium during the culture, or by contacting with the culture of the culture medium, living viable cells, or a treated product thereof.
培養中の場合は、培養開始時もしくは途中からTDPAを
添加して好気的に分解菌を培養しながら、 TDPAの分解を行わせる。また、分解菌を十分に生育させ
た培養物中にTDPAを添加して、好気的に分解してもよ
く、更に培養物から生菌体を回収し、菌体の高濃度懸濁
液として、これとTDPAを好気的に接触させて分解しても
よい。In the case of culturing, TDPA is decomposed aerobically by adding TDPA at the start of the culture or during the culture. In addition, TDPA may be added aerobically to the culture in which the degrading bacteria have been sufficiently grown, and the viable cells may be recovered from the culture to obtain a high-concentration suspension of the cells. , And TDPA may be aerobically contacted and decomposed.
次に、本発明を実施例により、さらに詳細に説明す
る。Next, the present invention will be described in more detail with reference to examples.
実施例１ TDPA 5g、NH4H2PO4 3g、KH2PO4 1.5g、NaHPO41.5g、M
gSO4・7H2O 0.1g、FeSO4・7H2O 0.01g、MnSO4・4H2O 0.
002g、酵母エキス0.2gを水道水１に溶解した培地をpH
7.2に調整したのち、その10mlを各試験管に入れ、120℃
で15分間蒸気殺菌し、表−７の自然界より単離した菌株
を接種し、30℃で１日振とう培養を行った。Example 1 TDPA 5 g, NH 4 H 2 PO 4 3 g, KH 2 PO 4 1.5 g, NaHPO 4 1.5 g, M
gSO 4 / 7H 2 O 0.1g, FeSO 4 / 7H 2 O 0.01g, MnSO 4 / 4H 2 O 0.
PH of a medium prepared by dissolving 002g and yeast extract 0.2g in tap water 1
After adjusting to 7.2, put 10ml into each test tube, 120 ℃
It was sterilized by steam for 15 minutes, inoculated with the strain isolated from the natural environment shown in Table 7, and shake-cultured at 30 ° C. for 1 day.
培地中のTDPA含量は、培養物より遠心分離で菌体を除
いて得た上清液をTotal Organic Carbon Analyzer（Bec
kman model 915）により測定した。また、菌の生育濃度
はOD 610nmの吸光度で測定した。The TDPA content in the medium was determined by removing the bacterial cells from the culture by centrifugation to obtain the supernatant liquid obtained using the Total Organic Carbon Analyzer (Bec
kman model 915). The growth concentration of the bacteria was measured by the absorbance at OD 610 nm.
結果を表−７に示す。 The results are shown in Table-7.
これより、各菌株はTDPAを唯一炭素源とした上記培地
で良好な生育を示し、培地中のTDPAの減少が確認され
た。From these results, it was confirmed that each strain showed good growth in the above medium using TDPA as the sole carbon source, and a decrease in TDPA in the medium was confirmed.
実施例２ 実施例１と同じ培地に複数の菌株を接種し、30℃で１
日振とう培養を行った。 Example 2 The same medium as in Example 1 was inoculated with a plurality of strains and incubated at 30 ° C for 1 hour.
Day shaking culture was performed.
結果を表−８に示す。 The results are shown in Table-8.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｃ１２Ｒ 1:38） (Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:07） (Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:05） (Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:20） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification code Internal reference number FI Technical display area C12R 1:38) (C12N 1/20 C12R 1:07) (C12N 1/20 C12R 1:05) (C12N 1/20 C12R 1:20)
Claims (1)
属、シュードモナス（Pseudomonas）属、バチルス（Bac
illus）属、アルカリゲネス（Alcaligenes）属、シトロ
バクター（Citrobacter）属およびフラボバクテリウム
（Flavobacterium）属に属し、チオジプロピオン酸を分
解する能力を有する微生物の中から選ばれた一種または
二種以上の微生物の培養液、分離生菌体、もしくはこれ
らの処理物とチオジプロピオン酸とを好気的に接触せし
めて、チオジプロピオン酸を分解することを特徴とする
微生物によるチオジプロピオン酸の分解方法。1. A microbacterium.
Genus, Pseudomonas, Bacillus
illus), Alcaligenes genus, Citrobacter genus and Flavobacterium genus, and one or more selected from microorganisms having the ability to decompose thiodipropionic acid Degradation of thiodipropionic acid by aerobically contacting thiodipropionic acid with a microbial culture solution, isolated viable cells, or a treated product of these, to decompose thiodipropionic acid by a microorganism. Method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8824288A JP2562347B2 (en) | 1988-04-12 | 1988-04-12 | Method for degrading thiodipropionic acid by microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8824288A JP2562347B2 (en) | 1988-04-12 | 1988-04-12 | Method for degrading thiodipropionic acid by microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01262997A JPH01262997A (en) | 1989-10-19 |
| JP2562347B2 true JP2562347B2 (en) | 1996-12-11 |
Family
ID=13937393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8824288A Expired - Fee Related JP2562347B2 (en) | 1988-04-12 | 1988-04-12 | Method for degrading thiodipropionic acid by microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2562347B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4219638A1 (en) * | 1992-06-16 | 1993-12-23 | Imre Dr Pascik | Process for the microbial degradation of substituted aromatics |
| CN102424174A (en) * | 2011-07-27 | 2012-04-25 | 河南省南街村（集团）有限公司 | Degradable plastic bag product |
-
1988
- 1988-04-12 JP JP8824288A patent/JP2562347B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01262997A (en) | 1989-10-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |