JPH04370046A - Solid medium - Google Patents

Abstract

PURPOSE:To obtain a solid medium having excellent appearance and air- permeability, free from necessity for maintenance work such as sprinkling and fertilization and useful for interior ornamental plant, etc., by adding and dispersing powdery activated carbon in a medium and solidifying the dispersion with a gelling agent. CONSTITUTION:A powdery activated carbon is added to and dispersed in a medium [in an amount of preferably 0.1-1%(W/V) based on the medium] and the dispersion is solidified with a gelling agent selected from agar and gellan gum to obtain the objective solid medium. The amounts of agar and gellan gum are preferably 0.4-4% and 0.1-2% based on the medium, respectively.

Description

[Detailed description of the invention]

0001

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a solid medium for indoor ornamental plants.

0002

BACKGROUND OF THE INVENTION Conventionally, indoor ornamental plants have generally been grown in culture soil, which has been placed in pots made of ceramic or the like. Recently, terrariums, which are made by filling a glass bottle with culture medium made of sand or soil and planting ornamental plants (adult or flowering plants) such as cactus or Saintpaulia, have also become available. However, these potting soils required occasional watering and fertilization. For this reason, over-watering and fertilizing often rots the roots and kills the plants, making maintenance difficult for the average person.

[0003] Therefore, the present inventors developed a gelling agent such as agar, which is commonly used in tissue culture, as an alternative to culture soil and does not require maintenance such as watering or fertilization. He came up with the idea of using a solid medium that had been solidified, and conducted extensive research using plants obtained through tissue culture of mini-varieties of Saintpaulia. However, although we tried various experiments by changing the concentration of nutrients in the medium and the amount of gelling agent added, we ended up with poor root hair development or roots growing along the surface of the medium and sitting down. As a result, good root development could not be expected. Therefore, the growth of stems and leaves was also poor.

0004

SUMMARY OF THE INVENTION An object of the present invention is to provide a solid culture medium for indoor ornamental plants that does not require maintenance such as watering or fertilization.

[0005]

[Means for Solving the Problems] The present invention provides a method for cultivating plants obtained by tissue culture of mini varieties of Saintpaulia, when using a so-called agar medium in which the medium is solidified with agar. This was done based on the knowledge that normal rooting is greatly promoted by adding and dispersing activated carbon powder.

That is, the present invention provides a solid medium characterized in that powdered activated carbon is added to the medium, dispersed therein, and solidified with a gelling agent.

[0007] The culture medium used in the present invention is not specified, but is a general medium determined depending on the target plant. For example, in the case of St.paulia of the Phylophyllaceae family, Prunus grass of the Prunaceae family, or what is called a wildflower, Murashige and Skoog are 1.
A completely synthetic medium for plant tissue culture (hereinafter referred to as MS medium) published in 1962 and B5 medium, a completely synthetic medium for plant cell culture published by Gamborg, are diluted 2 to 5 times and used as a basic medium. It is recommended to use it as a constituent nutrient (hereinafter referred to as the basic medium). In this case, sucrose is used as a carbon source to promote plant growth.
se) may be added in an amount of 0.1 to 5% (w/v). The pH of the medium is also determined by the target plant, but it is preferably slightly acidic.

[0008] As the activated carbon in the present invention, commercially available powdered carbon can be used because it is easily dispersed uniformly and is difficult to precipitate. In addition, the amount of activated carbon added is preferably 0.1 to 1% (w/v) based on the medium, preferably 0.
．． 3 to 0.5% (w/v) is good. Added amount of activated carbon is 0
．． If it is less than 1% (w/v), it will not have the effect of promoting rooting, and if it exceeds 1% (w/v), the binding force of the gelling agent will be weakened and it will not only be difficult to solidify. If an attempt is made to improve the appearance by taking advantage of the near-transparent color tone, which is one of the characteristics of this solid medium, the black color will stand out, which is undesirable.

[0009] Specific examples of the gelling agent in the present invention include agar and gellan gum. A feature of these gelling agents is that not only can the solidified product retain water for a long period of time, but also that the hardness of the medium can be freely adjusted by adjusting the amount added. In addition, the solidified product is nearly transparent, so it looks good as is, but it can be colored to create a variety of colors, so if it is placed in a transparent container such as a glass bottle, it can be adapted to a variety of interior decorations. become able to. For example, when using agar, the amount added is 0.4 to 4% (w/v) to the medium.
), preferably 0.5 to 1% (w/v). If the amount of agar added is less than 0.4% (w/v), the medium will become soft and its shape retention will be weakened, and if it exceeds 4% (w/v), it will become too hard, making it difficult for rooting to take place physically. This is not desirable as it has a negative impact. In addition, when using gellan gum, the amount added is 0.1 to 2% (
w/v), preferably 0.2-0.4% (w/v
) is better. The amount of gellan gum added is 0.1% (w/v)
If it is less than 2% (w/v) or more than 2% (w/v), the same result as in the case of agar will result, which is not preferable.

[0010] The method for producing the solid medium in the present invention is not particularly limited, and for example, a method for producing a solid medium used in tissue culture can be used, and examples include the following methods. First, necessary culture medium components are dissolved in water in a beaker or the like to a constant volume, and then the pH is adjusted to 5.5 to 6.0 using an aqueous potassium hydroxide solution, diluted hydrochloric acid, or the like. Next, after adding and mixing the gelling agent, the solution is heated in a microwave oven, a double boiler, etc., while periodically stirring with a glass rod, until the solution becomes transparent. The product obtained in this manner is dispensed into bottles in which powdered activated carbon has been previously filled, the bottles are tightly capped, and the bottles are sterilized by heating in an autoclave or the like. After sterilization, take out the bottle and shake it until the precipitated activated carbon is evenly dispersed, then leave it at room temperature to cool and solidify.

[0011]

[Example 1] Partially modified MS with addition of 10 g of sucrose
2-fold dilution of medium (hereinafter referred to as MS modified medium) 100
0ml was placed in a 2-liter beaker, and the pH was adjusted to 5.8 with 0.1N aqueous potassium hydroxide solution and 0.1N diluted hydrochloric acid. Next, after adding and mixing 7 g of agar, the beaker was placed in a microwave oven and heated while periodically stirring with a glass rod until the solution became transparent. The material obtained in this way was placed in 30 200 ml bottles each containing 1.5 g of powdered activated carbon.
After dispensing 1 liter each and sealing it, place it in an autoclave.
Heat sterilization was performed at 20°C for 15 minutes. After sterilization, the bottle was taken out from the autoclave, shaken until the precipitated activated carbon was uniformly dispersed, and then left at room temperature to cool and solidify. The composition of the MS modified medium is shown below. (Composition of MS modified medium (mg/l) Inorganic salts NH4NO3 1,650.0
KNO3 1,900.0
CaCl2・2H2O 440.0Mg
SO4・7H2O 370.0KH2P
O4 170.0Mn
SO4・4H2O 22.3H3B
O3 6
．． 2ZnSO4・7H2O 8
．． 6KI
0.83Na2MoO4・2H2O
0.25CuSO4・5H2O
0.025CoCl2・6H2O
0.025Na2EDTA
37.3FeSO4・7H2O
27.8 Organic compound myo-inositol 100.0 Glycine 2
．． 0 nicotinic acid
1.0 Pyridoxine Hydrochloride
0.5thiamine hydrochloric acid
0.5 biotin
0.05 folic acid
0.5

[0012] Sterile plants (with a grass span of approximately 3 cm) obtained by tissue culture of Little Sapphire, a mini variety of Saintpaulia, were placed in the solid medium in the bottle obtained as described above. After planting and sealing in a clean bench, the plants were cultured at 25°C for 40 days under 16-hour photoperiod and approximately 4000 lux lighting. as a result,
In all the media, good roots with well-developed root hairs were elongated into the media.

As a comparative example, cultivation was carried out under the same conditions as in the above Example except that activated carbon was omitted. As a result, in all the media, root hairs hardly developed, roots were discolored, and roots grew along the surface of the media and became uncomfortable.

[0014]

[Example 2] 2 of MS modified medium supplemented with 10 g of sucrose
1000 ml of the diluted solution was placed in a 2 liter beaker, and the pH was adjusted to 5.8 with 0.1 N aqueous potassium hydroxide solution and 0.1 N diluted hydrochloric acid. Next, after adding and mixing 2 g of gellan gum, the beaker was placed in a microwave oven and heated while periodically stirring with a glass rod until the solution became transparent. The following steps were carried out in the same manner as in Example 1.

[0015] A sterile plant (with a grass span of about 3 cm) obtained by tissue culture of Little Sapphire, a mini variety of Saintpaulia, was placed in the solid medium in the bottle obtained as described above. After planting and sealing in a clean bench, the plants were cultured at 25°C for 40 days under 16-hour photoperiod and approximately 4000 lux lighting. As a result, good roots with well-developed root hairs were elongated in all the media.

As a comparative example, cultivation was carried out under the same conditions as in the above Example except that activated carbon was omitted. As a result, in all the media, root hairs hardly developed, roots were discolored, and roots grew along the surface of the media and became uncomfortable.

[0017]

[Effects of the Invention] According to the present invention, it is possible to provide a solid culture medium for indoor ornamental plants that does not require maintenance such as watering or fertilization. Furthermore, when this solid medium is placed in a transparent container such as a glass bottle, it not only looks good due to its near-transparent color, but also can be colored to adapt to various interiors. moreover,
Since there is no need for watering or fertilization maintenance, it can be used in a variety of container shapes, even those with small openings such as bottle ships, and can also be used with a stopper that has good ventilation and does not allow germs to pass through. By maintaining the inside of the container in a sterile state, it can be completely maintenance-free without worrying about the invasion of mold or the like.

Claims (5)

[Claims] 1. A solid medium, characterized in that powdered activated carbon is added to the medium, dispersed therein, and solidified with a gelling agent. Claim 2: The amount of activated carbon added is 0.1 to the medium.
The solid medium according to claim 1, characterized in that the content is ~1% (w/v). 3. The solid medium according to claim 1, wherein the gelling agent is agar or gellan gum. Claim 4: The amount of agar added is 0.4 to 0.4 to the medium.
The solid medium according to claim 3, characterized in that the concentration is 4% (w/v). 5. The solid medium according to claim 3, wherein the amount of gellan gum added is 0.1 to 2% (w/v) based on the medium.