JPH0430834B2 - - Google Patents
Info
- Publication number
- JPH0430834B2 JPH0430834B2 JP62096072A JP9607287A JPH0430834B2 JP H0430834 B2 JPH0430834 B2 JP H0430834B2 JP 62096072 A JP62096072 A JP 62096072A JP 9607287 A JP9607287 A JP 9607287A JP H0430834 B2 JPH0430834 B2 JP H0430834B2
- Authority
- JP
- Japan
- Prior art keywords
- gpo
- solution
- added
- freeze
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000203 mixture Substances 0.000 claims description 12
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 claims description 5
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 claims description 5
- 239000011714 flavin adenine dinucleotide Substances 0.000 claims description 5
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 claims description 5
- 108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 claims description 4
- 102000000587 Glycerolphosphate Dehydrogenase Human genes 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 239000006172 buffering agent Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- GNGACRATGGDKBX-UHFFFAOYSA-N dihydroxyacetone phosphate Chemical compound OCC(=O)COP(O)(O)=O GNGACRATGGDKBX-UHFFFAOYSA-N 0.000 description 2
- 108010054790 glycerol-3-phosphate oxidase Proteins 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- AWUCVROLDVIAJX-VKHMYHEASA-N sn-glycerol 1-phosphate Chemical compound OC[C@H](O)COP(O)(O)=O AWUCVROLDVIAJX-VKHMYHEASA-N 0.000 description 1
- HFQQZARZPUDIFP-UHFFFAOYSA-M sodium;2-dodecylbenzenesulfonate Chemical compound [Na+].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O HFQQZARZPUDIFP-UHFFFAOYSA-M 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9607287A JPS63263082A (ja) | 1987-04-17 | 1987-04-17 | 安定化されたグリセロリン酸オキシダ−ゼ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9607287A JPS63263082A (ja) | 1987-04-17 | 1987-04-17 | 安定化されたグリセロリン酸オキシダ−ゼ |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14588180A Division JPS5768788A (en) | 1980-10-17 | 1980-10-17 | Stablized glycerophosphate oxidase composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63263082A JPS63263082A (ja) | 1988-10-31 |
| JPH0430834B2 true JPH0430834B2 (no) | 1992-05-22 |
Family
ID=14155205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9607287A Granted JPS63263082A (ja) | 1987-04-17 | 1987-04-17 | 安定化されたグリセロリン酸オキシダ−ゼ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63263082A (no) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5768788A (en) * | 1980-10-17 | 1982-04-27 | Toyobo Co Ltd | Stablized glycerophosphate oxidase composition |
-
1987
- 1987-04-17 JP JP9607287A patent/JPS63263082A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5768788A (en) * | 1980-10-17 | 1982-04-27 | Toyobo Co Ltd | Stablized glycerophosphate oxidase composition |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63263082A (ja) | 1988-10-31 |
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