JPH0428352B2 - - Google Patents
Info
- Publication number
- JPH0428352B2 JPH0428352B2 JP18019386A JP18019386A JPH0428352B2 JP H0428352 B2 JPH0428352 B2 JP H0428352B2 JP 18019386 A JP18019386 A JP 18019386A JP 18019386 A JP18019386 A JP 18019386A JP H0428352 B2 JPH0428352 B2 JP H0428352B2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- settling zone
- cells
- zone
- suspension
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000004113 cell culture Methods 0.000 claims description 26
- 238000004114 suspension culture Methods 0.000 claims description 26
- 238000005192 partition Methods 0.000 claims description 24
- 230000010412 perfusion Effects 0.000 claims description 5
- 210000004027 cell Anatomy 0.000 description 50
- 239000000243 solution Substances 0.000 description 39
- 239000001963 growth medium Substances 0.000 description 23
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 21
- 229910052760 oxygen Inorganic materials 0.000 description 21
- 239000001301 oxygen Substances 0.000 description 21
- 239000000725 suspension Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 13
- 239000007788 liquid Substances 0.000 description 11
- 238000012258 culturing Methods 0.000 description 8
- 230000005484 gravity Effects 0.000 description 8
- 238000003756 stirring Methods 0.000 description 7
- 238000004062 sedimentation Methods 0.000 description 6
- NBVXSUQYWXRMNV-UHFFFAOYSA-N fluoromethane Chemical compound FC NBVXSUQYWXRMNV-UHFFFAOYSA-N 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 239000012531 culture fluid Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000007667 floating Methods 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000012736 aqueous medium Substances 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000010907 mechanical stirring Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 210000000628 antibody-producing cell Anatomy 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000002473 artificial blood Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18019386A JPS6336774A (ja) | 1986-08-01 | 1986-08-01 | 細胞培養装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18019386A JPS6336774A (ja) | 1986-08-01 | 1986-08-01 | 細胞培養装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6336774A JPS6336774A (ja) | 1988-02-17 |
| JPH0428352B2 true JPH0428352B2 (fr) | 1992-05-14 |
Family
ID=16079022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18019386A Granted JPS6336774A (ja) | 1986-08-01 | 1986-08-01 | 細胞培養装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6336774A (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9131902B2 (en) | 2004-02-05 | 2015-09-15 | Earlysense Ltd. | Prediction and monitoring of clinical episodes |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0398572A (ja) * | 1989-09-13 | 1991-04-24 | Teijin Ltd | 細胞培養装置および方法 |
| US6376445B1 (en) | 1997-08-14 | 2002-04-23 | Procter & Gamble Company | Detergent compositions comprising a mannanase and a protease |
| GB201015672D0 (en) | 2010-09-20 | 2010-10-27 | Unilever Plc | Improvements relating to fabric treatment compositions comprising targeted benefit agents |
-
1986
- 1986-08-01 JP JP18019386A patent/JPS6336774A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9131902B2 (en) | 2004-02-05 | 2015-09-15 | Earlysense Ltd. | Prediction and monitoring of clinical episodes |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6336774A (ja) | 1988-02-17 |
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