JPH04281789A - Prepared substance containing immobilized microbial cell and its production - Google Patents

Abstract

PURPOSE:To obtain the subject immobilized substance having high chemical stability; safety, strength and durability by including and immobilizing microbial cells in a natural polymeric gel, etc., forming a microorganism-containing gel layer, enclosing the aforementioned gel layer with a composition of a synthetic polymer, etc., and curing the composition. CONSTITUTION:Microbial cells are cultured at 37 deg.C temperature by shaking culture to provide a culture solution, which is then centrifuged to collect the microbial cells. The collected microbial cells are subsequently suspended in a physiological saline solution to prepare a microbial cell suspension. The resultant microbial cell suspension in an amount of 10% based on an aqueous solution of 2% sodium alginate is added and mixed therewith, extended to 2mm thickness in the form of a flat plate, dipped in a 0.05M aqueous solution of CaCl2 and gelatinized. Thereby, a microorganism- containing gel layer (B) consisting essentially of a natural polymeric gel is formed and then enclosed with a composition consisting essentially of a synthetic polymer (e.g. polymethyl methacrylate). The aforementioned composition consisting essentially of the synthetic polymer is subsequently cured to form cured enclosing layers (A). As a result, the objective immobilized microbial cells are obtained.

Description

[Detailed description of the invention]

0001

[Industrial Application Field] The present invention is directed to the decomposition of environmental pollutants,
The present invention relates to preparations containing immobilized microbial cells that are used for material conversion in processing and production processes, and methods for producing the same.

0002

[Prior Art] Conventionally, a method using a so-called biofilter has been used as a method for deodorizing and removing organic exhaust gases and organic waste liquids discharged as industrial waste, such as alcohols, carboxylic acids, aldehydes, ketones, and esters. (
For example, “VT-Biofilter, BHL J
J W”, Ing. Breau Van Tong
Environ Technol, pp.
．． 358-360, 1987) are known.

[0003] As described above, in order to utilize microorganisms for material conversion in decomposition, treatment, and production processes of environmental pollutants, it has been conventionally possible to immobilize microorganisms by using natural or synthetic polymers. Most of the methods were based on the so-called entrapping immobilization method, in which microorganisms are embedded in gel (
For example, Ichiro Chibata, "Immobilized Biocatalyst", pp. 6
9-79, Kodansha, 1986, etc.).

[0004] In this method, various immobilization carriers and immobilization operations have been studied in order to prevent the activity of microorganisms from decreasing during the process of immobilizing microorganisms, subsequent storage, and use, and the selection is made depending on the purpose of use. has been done. In other words, from the viewpoint of the difficulty of the immobilization operation, the price of the immobilization carrier, and the fact that harsh conditions are not favorable for the microorganisms to be immobilized, the strength and lifespan of the immobilization carrier are important. ,
Various studies have been conducted on the permeability of reaction substrates, growth of microorganisms within the carrier, and activity of immobilized carriers.

For example, natural polymers such as calcium alginate and carrageenan are known as carriers that immobilize microorganisms and maintain long-term performance, and are reported to have excellent effects. I. A. Veliky, R. E
．． Williams, “Biotechnol.
Lett. ”, vol. 3, pp. 275,
In 1981, a method was reported in which microorganisms were immobilized on calcium alginate by mixing a bacterial cell suspension and an aqueous sodium alginate solution and then contacting the mixture with an aqueous CaCl2 solution. Also, T. Tosa et al., “Biotechn
ol. Bioeng. ”, vol. 21, pp.
．． 133, 1979, a method is reported in which microorganisms are immobilized on carrageenan by mixing a bacterial cell suspension and an aqueous κ-carrageenan solution and then cooling the mixture to 30 to 60°C.

[0006] Furthermore, methods using synthetic polymers such as polyacrylamide and polyvinyl alcohol are generally used. For example, I. Ch
Ibata et al., “Microbiol.”, vol.
27, pp. 878, 1974, reported a method for immobilizing microorganisms on polyacrylamide by adding a polymerization accelerator and an initiator to a mixture of a bacterial cell suspension, an acrylamide monomer, and an acrylic acid derivative crosslinking agent and mixing them. . Furthermore, in JP-A-61-139385, after mixing a bacterial cell suspension and a polyvinyl alcohol aqueous solution, -20
A method is disclosed in which microorganisms are immobilized on polyvinyl alcohol by leaving it under low temperature conditions of ~-80°C.

[0007]

[Problems to be Solved by the Invention] When incorporating immobilized microorganisms into a device, there are many issues such as pressure resistance in the flow path, stability against a wide range of chemicals, and safety against contaminating microorganisms. It is essential to satisfy the requirements.

However, in the above-mentioned conventional method of immobilizing microorganisms using natural polymers such as calcium alginate and carrageenan, the carrier is a polysaccharide or a gel that has the property of being hydrolyzed by acids. Because there is
It has insufficient acid resistance and mechanical strength, and cannot be used depending on the amount and type of chemical substances to be treated.
It could hardly be said that it could withstand repeated and long-term use.

On the other hand, with the method of immobilizing microorganisms using synthetic polymers such as polyacrylamide and polyvinyl alcohol, toxicity becomes a problem depending on the usage environment, and furthermore, these synthetic polymers cannot be heated. Generally, processing is performed by adding plasticizers, solvents, etc., but this processing method kills microorganisms and reduces activity, so it cannot be used, and it is difficult to mold into the desired shape, making it difficult to use. It was difficult to create a form that suited the purpose.

[0010] In addition, other conventional entrapping immobilization carriers have the same problems as these. Furthermore, in all the conventional microorganism immobilization methods mentioned above, since they are gels, they lack mechanical strength and microbial cells easily leak out of the gel in a short period of time, resulting in microbial contamination and immobilized materials. This causes problems such as a limit on the number of times of use, and furthermore, microorganisms proliferate within the gel, causing the gel to disintegrate and become unusable.

[0011]

[Means for Solving the Problems] The present invention has been made in view of the above-mentioned problems of the prior art, and aims to improve stability against a wide range of chemical substances, etc.
A preparation containing immobilized microorganisms that is safe against contaminating microorganisms, has sufficient mechanical strength, has no toxicity, can be easily prepared, and can withstand repeated and long-term use, and its preparations. The purpose is to provide a manufacturing method.

In order to achieve this object, the preparation containing immobilized microbial cells of the present invention comprises a microorganism-containing gel layer mainly composed of a natural polymer gel containing immobilized microbial cells and a synthetic polymer gel layer. The composition is composed of a hardened enveloping layer which envelops and hardens the microorganism-containing gel layer, and the cured enveloping layer envelops the entire microorganism-containing gel layer to form the gel. The structure is characterized in that there are no exposed parts of the layer.

[0013] Further, according to the present invention, such a preparation containing immobilized microbial cells comprises (1) containing and immobilizing microbial cells in a composition containing a natural polymer gel as a main component; (2) preparing a gel layer containing microorganisms;
The microorganism-containing gel layer is encapsulated with a composition whose main component is a synthetic polymer, and the composition whose main component is a synthetic polymer is cured to form a cured encapsulation layer. be able to.

[0014]

[Example] A. Preparation method of microbial cell immobilization gel As the immobilization gel for immobilizing microbial cells used in the present invention, any well-known natural polymer that can immobilize microorganisms can be used. Calcium alginate, carrageenan, agar, gelatin, etc. can be used, to name a few.

[0015] In addition, the immobilization method should be used after considering its concentration, adjuvants to be added, etc. as appropriate depending on the type of natural polymer used. Depending on the physiological properties of the microorganisms to be immobilized, microbiological considerations such as temperature, pH, medium components, and stirring/mixing method should be satisfied during immobilization, so that the immobilized microorganisms retain their activity. It is essential to do so.

Example 1 Preparation of gel film with immobilized microorganism Brevibacterium sp. having acetone decomposition ability.
MEOH-4 Bacteria (Brevibacterium)
sp. MEOH-4) (Bacteria deposited at the Institute of Microbial Technology, Agency of Industrial Science and Technology (FER)
M P-11047)) was cultured with shaking at 37°C in nutrient broth (manufactured by Difco). The resulting culture solution was centrifuged at 5,000 rpm for 5 minutes to collect the bacterial cells, and the cells were added to physiological saline. to prepare a bacterial cell suspension. 10% of this bacterial cell suspension was added to a 2% aqueous sodium alginate solution and mixed. After spreading this on a flat plate to a thickness of 2 mm,
It was immersed in a container containing an aqueous solution of M Cacl2 for 5 minutes to form a gel. Thereafter, it was cut into disks with a diameter of 2.5 cm and mixed with nutrient broth (manufactured by Difco).
The cells were immersed in water and cultured for 5 days (static culture at 37°C) to fix the growth.

B. Preparation of a hardened encapsulant layer for enclosing a microorganism-containing gel layer Various resins are blended into the polymer composition used for the cured encapsulant layer for enclosing the microorganism-immobilized gel prepared as described above. Is possible. However, if we take into account all of the intended objectives, such as maintaining hydrophilicity (water absorption), maintaining strength, retaining and propagating bacteria, and maintaining activity, we prefer those having an acroyl group, e.g. Methyl (meth)acrylate, ethyl (meth)acrylate, etc. can be used, but a mixture of N-methoxymethylated nylon and/or urethane acrylate and 2-hydroxyethyl (meth)acrylate is preferred. In this case, from the viewpoint of strength and hydrophilicity (water absorption) of the final preparation containing immobilized microbial cells, N-methoxymethylated nylon and/or urethane acrylate and 2-hydroxyethyl (meth) ) The total weight of acrylate resin is
It is desirable that the content be 50% by weight or more of the total weight of the preparation.

[0018] Furthermore, when dissolving and blending N-methoxymethylated nylon and/or urethane acrylate with 2-hydroxyethyl (meth)acrylate, since these resins have acrylic activity, The temperature must be below 100°C, preferably 80°C. Furthermore, in this case, considering the solubility, viscosity during use, gelation, etc. of N-methoxymethylated nylon and/or urethane acrylate,
- A practically suitable concentration is such that the content of methoxymethylated nylon and/or urethane acrylate is 30% or less, preferably 3 to 30% by weight, of the weight of the cured encapsulation layer.

On the other hand, when flexibility and water resistance of the final preparation are required, a group consisting of 2-hydroxy-3-phenoxypropyl acrylate, phenoxy acrylate, dimethylaminoethyl acrylate, polyethylene glycol methacrylate, and epoxy acrylate may be used. It is also possible to appropriately add one or more resins selected from the following.

[0020] In addition, natural polymer acrylates such as cellulose acrylate and sucrose acrylate may be appropriately blended as required.

Furthermore, in order to facilitate mixing of the resins,
It is also possible to add water, alcohols, oils and fats, inorganic substances, dyes, pigments, etc.

To the mixture thus prepared is added a commonly used polymerization initiator such as benzoin ethyl ether in an amount of about 1%. Then, in order to encapsulate the microbial cell immobilization gel, this synthetic polymer composition is applied and cast on the surface of the gel body, and the acrylic resin is photocured and immobilized by irradiation with active light. Alternatively, the microbial cell-immobilized gel is embedded in a resin of a synthetic polymer composition and then photocured. In this case, various conditions such as photocuring time and wavelength depend on the type of resin and blending ratio, but the important thing is to ensure that the temperature of the gel body with immobilized microorganisms does not exceed 50°C. It is necessary to choose carefully.

Specifically, in addition to the method of casting on a glass plate and fixing it by photocuring (Fig. 2), in place of the glass plate, in a mold such as a semicircular sphere or a spiral shape, By pouring the synthetic polymer composition and photocuring it, it is possible to prepare a preparation containing the immobilized microbial cells of the present invention in any desired shape, such as a spherical shape (FIG. 3).

Example 2 Preparation of a preparation containing immobilized microbial cells (formation of a hardened encapsulant layer enclosing the microorganism-containing gel layer) 10 g of N-methoxymethylated nylon was
g of 2-hydroxyethyl (meth)acrylate was dissolved in advance at 80°C, 50 g of dimethylaminoethyl acrylate was added and mixed, and 1.5 g of dimethylaminoethyl acrylate was added as a polymerization initiator.
A photosensitive resin was prepared by adding and mixing g of benzoin ethyl ether. This resin was cast onto a 2 mm thick glass plate to a thickness of 0.2 mm, and 10 mm was cast from the top and bottom.
It was irradiated with near ultraviolet light (center wavelength: 350 nm) for a minute to polymerize and solidify.

[0025] At this time, polymerization is hindered on the surface that comes into contact with air, and the surface layer remains viscous without solidifying, so the microorganism-immobilized gel film prepared in Example 1 above is placed on top of this. , and then apply the aforementioned photosensitive resin on top of this.
After casting to a thickness of 1 mm, a transparent polyester film (0.1 mm thick) was placed on the resin surface, and near-UV irradiation (center wavelength 350 nm) was applied from the top and bottom for 10 minutes.
nm) to polymerize and solidify to complete photocuring.

Example 3 Activity of a preparation containing immobilized microbial cells Next, the preparation containing immobilized microbial cells prepared in Example 2 above was tested so that the microorganism-immobilized gel film would not be exposed through the cut. For this, use 5 discs cut out with a diameter of 5 cm, and add 0.5% acetone and 0.005% acetone.
% of yeast extract (manufactured by Difco) (pH 6.5) and incubated at 25°C.
Shaking culture for days (92 times/min, amplitude 6cm)
Then, the disappearance of acetone and changes in the properties of the immobilized product were measured. The results are shown in Table 1 below.

[0027]

[Table 1]

As is clear from Table 1, acetone completely disappeared over time. In addition, the immobilized product did not show any peeling, collapse, or other changes before and after use, and could be repeatedly reused, with almost no decrease in activity observed.

Example 4 20 g of urethane acrylate, 30 g of 2-hydroxyethyl (meth)acrylate, and 50 g of dimethylaminoethyl acrylate were dissolved and mixed with stirring at 80°C, and 1 g of 2-hydroxyethyl acrylate was added as a polymerization initiator. Hydroxy-2-ethyl-1-phenyl-1-one was added and mixed to prepare a photosensitive resin liquid. This resin liquid was cast onto a 0.1 mm thick polyester film to a thickness of 0.2 mm, placed on a glass plate, and irradiated with near ultraviolet light (from the top and bottom) for 5 minutes. (center wavelength 350 nm), and the polyester side was solidified to polymerize and solidify.

The microorganism-immobilized gel film prepared in Example 1 above was placed on top of this, and the photosensitive resin described above was cast onto this to a thickness of 1 mm.
Transparent polyester film (0.1 mm) on the resin surface
(thickness of 350 nm) and irradiated with near ultraviolet rays (center wavelength 350 nm) from the top and bottom for 10 minutes to polymerize and solidify, completing photocuring.

[0031] Next, in order to prevent the microorganism-immobilized gel film from being exposed from the cut end, five disk-shaped sheets with a diameter of 5 cm were used, and acetone was added at 0.5% and 0.0%.
Pour into 100 ml of an aqueous solution containing 0.5% yeast extract (manufactured by Difco) (pH 6.5) and store at 25°C.
Shaking culture for 7 days (92 times/min, amplitude 6c)
m) The disappearance of acetone and changes in the properties of the immobilized product were measured. The results are shown in Table 2 below.

[0032]

[Table 2]

As is clear from Table 2, acetone completely disappeared with the passage of time. In addition, the immobilized product did not show any peeling, collapse, or other changes before and after use, and could be repeatedly reused, with almost no decrease in activity observed.

Example 5 20 g of urethane acrylate, 30 g of 2-hydroxyethyl (meth)acrylate, and 50 g of dimethylaminoethyl acrylate were dissolved and mixed with stirring at 80°C, and 1 g of 2-hydroxyethyl acrylate was added as a polymerization initiator. Hydroxy-2-ethyl-1-phenyl-1-one was added and mixed to prepare a photosensitive resin liquid. This resin liquid was cast onto a 0.1 mm thick polyester film to a thickness of 0.2 mm, placed on a glass plate, and irradiated with near ultraviolet rays from the top and bottom for 5 minutes. (center wavelength 350 nm) to solidify the polyester surface side and polymerize and solidify.

The microorganism-immobilized gel film prepared in Example 1 above was placed on top of this, and the photosensitive resin described above was cast onto it to a thickness of 1 mm.
Transparent polyester film (0.1 mm) on the resin surface
(thickness of 350 nm) and irradiated with near ultraviolet rays (center wavelength 350 nm) from the top and bottom for 10 minutes to polymerize and solidify, completing photocuring.

Next, in order to prevent the microorganism-immobilized gel film from being exposed from the cut end, five discs each having a diameter of 5 cm were cut out and treated with 0.5% acetone and 0.5% acetone.
0.005% yeast extract (manufactured by Difco) (pH 6.5), and
Shaking culture at 5℃ for 7 days (92 times/min, amplitude
6 cm) to measure the disappearance of acetone and changes in the properties of the immobilized product. The results are shown in Table 3 below.

[0037]

[Table 3]

As is clear from Table 3, acetone completely disappeared over time. In addition, the immobilized product did not show any peeling, collapse, or other changes before and after use, and could be repeatedly reused, with almost no decrease in activity observed.

Comparative Example 1 Properties of Conventional Preparation Experiment 1 In order to examine the properties of an immobilized product obtained by a conventional general comprehensive immobilization operation, an immobilized product was prepared. That is, 20% N-methoxymethylated nylon and 80% 2-hydroxyethyl (meth)acrylate were used as synthetic polymers.
Resin (A) was prepared by adding and mixing an equivalent amount of 2-hydroxy-3-phenoxypropyl acrylate to a mixture of 1% (dissolved and stirred at 80°C) and 1% of benzoin ethyl ether. Next, this resin (
A) 1.25 times, 2.0 times, 3.5 times, respectively.
Add and mix at a ratio of 0.1M and 8.0x.
After dropping it into a calcium chloride aqueous solution to gel it into a spherical shape, it was immediately irradiated with near ultraviolet light for 10 minutes (center wavelength
Spheres were prepared by photocuring (350 nm). The results are shown in Table 4 below.

[0040]

[Table 4]

As is clear from this table, as the proportion of sodium alginate increases, the interior of the resin (A) becomes non-uniform, and conversely, as the amount of resin (A) increases, it becomes difficult to harden (gel). Ta. Moreover, when immersed in water or culture medium, it collapsed regardless of the mixing ratio.

Comparative Example 2 Characteristic Experiment 2 of Conventional Preparation The procedure was the same as in Example 1 except that no microbial cells were included, and calcium alginate gel (B) was used as the natural polymer.
) was prepared in the form of a sheet and placed on top of the resin (A) which had been photocured into a sheet in the same manner as in Example 2, and the resin (A) was poured over it for 10 minutes. The material was photocured by near-field ultraviolet irradiation (center wavelength 350 nm) to create a sheet consisting of three layers as shown in Figure 1. In order to investigate the properties of this sheet, 0.5% of acetone was added.
% and 0.005% of yeast extract (manufactured by Difco). The results are shown in Table 5 below.

As is clear from this table, when immersed in water or a medium, layer (B) elutes and collapses, and each layer peels off.

Comparative Example 3 Characteristic experiment of the preparation according to the present invention A gel body (B) of calcium alginate as a natural polymer was prepared in the same manner as in Example 1 except that it did not contain microbial cells, and was shaped into a sheet. The above (A)
Resin (A) was formed into a sheet in the same manner as in Example 2, placed on top of the photocured material, poured resin (A) on top to cover it, and irradiated with near ultraviolet light (center wavelength 350 nm) for 10 minutes.
) and photocuring, the cut surface of the sheet (B) is not exposed, that is, the gel body (B) is completely resin (A).
(FIG. 2), and various experiments were conducted in the same manner as in Comparative Example 2 to examine the characteristics. The results are shown in Table 5 below.

[0045]

[Table 5]

As is clear from this table, no elution, deformation, or collapse occurred in layer (B) even when immersed in water or medium.

[0047] As described above, in the case of conventional comprehensive immobilization in Comparative Examples 1 and 2, the layer (B) containing microbial cells and to be immobilized may elute or disintegrate, and peeling of each layer may occur. , which is unacceptable for use, whereas in the immobilized preparation according to the invention, as shown in Comparative Example 3,
Since the (B) layer containing microbial cells and to be immobilized is entirely encapsulated (protected) by the light-cured (A) layer,
It can be seen that the method and preparation of the present invention are effective as a microorganism immobilized product without elution or disintegration.

[0048]

[Operation/Effect] According to the preparation containing immobilized microbial cells and the method for producing the same according to the present invention, it has stability against a wide range of chemical substances, safety against contaminating microorganisms, sufficient mechanical strength, etc. Moreover, it is possible to provide a preparation containing immobilized microbial cells that does not pose a problem of toxicity, can be easily prepared, and can withstand repeated and long-term use, and a method for producing the same. Specifically, (1) the microorganisms inside the gel are protected by the synthetic polymer that encapsulates the gel, so it will last for a long time even under harsh conditions when installed in a processing device. It is possible to maintain activity,
It is extremely effective for industrial applications such as the treatment of environmental pollutants and the conversion of substances used as biocatalysts.

(2) Since the synthetic polymer used for encapsulation has an acroyl group, it can be photocured in a short time, with almost no temperature change, and there is no effect on the microorganisms to be immobilized.

(3) Immobilization of microbial cells Since N-methoxymethylated nylon is contained as a synthetic polymer material for encapsulating the gel layer, it is possible to encapsulate the gel without compressing it. The microorganisms in the gel are stable, contaminants and raw materials to be treated can easily seep in from the outside of the immobilized material, and the products after reaction can quickly leach out of the gel and move out of the system. It is valid.

(4) Furthermore, the specific gravity can be adjusted by appropriately selecting and adding a synthetic polymer composition that is encapsulated and hardened, making it possible to incorporate it into the equipment and adapt it to the processing environment. . This is an extremely excellent invention that has numerous functions and effects such as.

[Brief explanation of the drawing]

FIG. 1 is an enlarged cross-sectional view of a conventional immobilized product.

FIG. 2 is an enlarged cross-sectional view of one embodiment of the immobilized product of the present invention.

FIG. 3 is an enlarged sectional view of another embodiment of the immobilized product of the present invention.

[Explanation of symbols]

A...cured coating layer B...Gel layer with immobilized microorganisms

Claims (13)

[Claims] Claim 1: A microorganism-containing gel layer consisting of a natural polymer gel containing immobilized microbial cells as a main component, and a composition containing a synthetic polymer as a main component, which encapsulates and hardens the microorganism-containing gel layer. An immobilized microorganism, characterized in that the hardened enveloping layer is configured to enclose the entire microorganism-containing gel layer so that no exposed portion of the gel layer is present. Preparation containing bacterial cells. 2. The preparation containing immobilized microbial cells according to claim 1, wherein the synthetic polymer is mainly a synthetic polymer having an acroyl group. 3. The synthetic polymer comprises N-methoxymethylated nylon and/or urethane acrylate;
A preparation containing immobilized microbial cells according to claim 1 or 2, characterized in that it contains a mixture of -hydroxyethyl (meth)acrylate. 4. Immobilization according to claim 3, characterized in that the content of the N-methoxymethylated nylon and/or urethane acrylate is 3 to 30% by weight of the weight of the cured encapsulation layer. A preparation containing microbial cells. 5. The synthetic polymer further comprises one or more selected from the group consisting of 2-hydroxy-3-phenoxypropyl acrylate, phenoxy acrylate, dimethylaminoethyl acrylate, polyethylene glycol methacrylate, and epoxy acrylate. The preparation containing immobilized microbial cells according to claim 3 or 4, characterized in that it contains a synthetic resin. 6. The composition according to claim 1, wherein the composition containing a synthetic polymer as a main component further contains a natural polymer such as cellulose acrylate or sucrose acrylate. A preparation containing immobilized microbial cells. 7. A method for producing a preparation containing immobilized microbial cells, comprising the following steps: (1) containing microbial cells in a composition containing a natural polymer gel as a main component; Step of immobilizing and preparing a microorganism-containing gel layer (
2) By using a composition whose main component is a synthetic polymer,
A preparation containing immobilized microorganism cells, comprising the steps of encapsulating the microorganism-containing gel layer and curing the composition containing the synthetic polymer as a main component to form a cured encapsulation layer. How things are manufactured. 8. The method for producing a preparation containing immobilized microbial cells according to claim 7, wherein the synthetic polymer is mainly a synthetic polymer having an acroyl group. 9. The synthetic polymer comprises N-methoxymethylated nylon and/or urethane acrylate;
A method for producing a preparation containing immobilized microbial cells according to claim 7 or 8, characterized in that it contains a mixture of -hydroxyethyl (meth)acrylate. 10. Immobilization according to claim 9, characterized in that the content of the N-methoxymethylated nylon and/or urethane acrylate is 3 to 30% by weight of the weight of the cured encapsulation layer. A method for producing a preparation containing microbial cells. 11. The synthetic polymer further comprises one or more selected from the group consisting of 2-hydroxy-3-phenoxypropyl acrylate, phenoxy acrylate, dimethylaminoethyl acrylate, polyethylene glycol methacrylate, and epoxy acrylate. 11. A method for producing a preparation containing immobilized microbial cells according to claim 9 or 10, characterized in that it contains a synthetic resin. 12. The composition according to claim 7, wherein the composition containing a synthetic polymer as a main component further contains a natural polymer such as cellulose acrylate or sucrose acrylate. A preparation containing immobilized microbial cells. 13. The preparation containing immobilized microbial cells according to claim 7, wherein the composition containing the synthetic polymer as a main component is cured by photocuring. manufacturing method.