JPH04237485A - Regulator for jetting speed of jetter for treating cell - Google Patents

Abstract

PURPOSE:To enable regulation of the jetting speed. CONSTITUTION:A tank 35 capable of regulating the volume is provided between a gas producing part 21 and a piston 23. Thereby, since a gas from the gas producing part 21 enters the tank 35, the pressure at the back of the piston 23 is regulated according to the volume of the tank 35. As a result, the speed of the piston 23, i.e., the jetting speed of a cell treating material 24 is regulated.

Description

[Detailed description of the invention]

0001

[Industrial Application Field] The present invention relates to a cell processing injection device used for causing transformation in cells.
The present invention relates to an injection rate adjustment device used for adjusting injection rate. For example, a cell processing injection device sprays a cell processing material such as fine metal powder attached with genetic material at high speed,
It is used to inject genetic material into cells by applying it to a sample containing cells, or to cause cell fusion by injecting cells at high speed.

0002

[Prior Art] A cell processing injection device uses gunpowder, compressed air, or the like to accelerate a plastic piston to which a cell processing material is attached at high speed, and causes it to collide with a plate or the like having small holes to release the cell processing material. It is designed to be sprayed.

0003

[Problems to be Solved by the Invention] This device does not have an appropriate means for adjusting the speed of the piston or cell processing material, and there is no method for changing the amount of gunpowder in devices using explosives, or in devices using high pressure gas. There is only a way to adjust the gas pressure. Therefore, speed adjustment is difficult and the adjustment range is narrow.

SUMMARY OF THE INVENTION Therefore, an object of the present invention is to provide an adjusting device that can easily adjust speed over a wide range.

[0005]

[Means for Solving the Problems] The present invention includes a gas generating section that instantaneously generates high-pressure gas, and a piston that is connected to the gas generating section and has a cell processing material attached to its tip. A cell that has a cylinder that advances the piston toward the tip, and an injection section that has a small hole and is provided on the tip or extension of the tip of the cylinder and that causes the piston to collide and spray the cell treatment material from the small hole onto the sample. An injection device for cell processing, which is provided with a tank between a gas generation part and a piston in a cylinder, the volume of which can be adjusted, and which receives and stores a portion of the gas flowing into the cylinder. This is an injection speed adjustment device.

Furthermore, the regulating device of the present invention may include a gas reservoir chamber and a resistor that applies resistance to the flow of gas from the gas reservoir chamber to the piston, between the gas generating section and the piston. .

[0007]

[Embodiments] Hereinafter, embodiments illustrating the present invention will be described in detail.

FIGS. 1 and 2 show a cell processing injection device 11 equipped with an injection speed adjustment device 10. As shown in FIG. This injection device 11 is
A removable case 13 is placed on the base 12 to cover the whole body, and the air inside is sucked in from the suction port 14 with a vacuum pump (not shown), and the whole interior is reduced in pressure. can do.

A column 15 is provided on the side of the platform 12.
A holder 16 is attached to this so that its vertical position can be adjusted by operating a cap screw 17.

The holder 16 supports a cylinder 18, and inside the upper end of the cylinder 18 is a blank cartridge 1 containing gunpowder.
9 can be loaded, and an ignition mechanism 20 for loading and igniting this blank cartridge 19 is provided on the upper part of the cylinder 18, and the blank cartridge 19 and the ignition mechanism constitute a gas generating section 21. Note that the ignition mechanism 20 is ignited by a solenoid, and the operation is performed from outside the case 13.

A cylinder 22 is removably screwed to the tip of the cylindrical body 18. This cylinder 22 accommodates a piston 23 inside. The piston 23 is made of plastic, has a rib protruding around its rear part, presses the inner surface of the cylinder 22, and maintains its position, and has a recess formed at its tip.
The cell treatment material 24, for example, fine tungsten powder to which genetic material is attached, is attached here by the surface tension of water.

A base portion of a front cover 25 is detachably attached to the tip or lower end of the cylinder 22 with a bayonet screw. This front cover 25 is
2, and covers the sample 26 placed on the cylinder 22.
The injection part 27 is held between the front end of the holder and the front end of the holder. This injection part 27 has a small hole 28 , and the piston 23 collides with the cell treatment material 24 to inject the cell treatment material 24 from the small hole 28 .

Near the tip of the cylinder 22 is an exhaust hole 29,
..., 30, ... are provided, and exhaust holes 31, ... that communicate with the exhaust holes 30, ... are also provided at the base of the front cover 25.

In the cell injection device 11 having the above configuration,
An injection speed adjusting device 10 having the following configuration is provided.

[0015] This adjustment device 10 mainly has outflow holes 32,
... and a tank 35 formed by an adjustment ring 33 and a movable ring 34. The outflow holes 32, . . . are formed on the circumferential surface of the cylindrical body 18 near the blank cartridge 19. Adjustment ring 33
has a substantially inverted cup shape, has a threaded groove 36 on its inner surface, and is rotatably fitted into a circumferential groove 37 formed between the cylindrical body 18 and the holder 16. The movable ring 34 fits on the outer periphery of the cylinder 22 so as to be movable up and down, and is screwed into the threaded groove 36 to form a tank 35 between the movable ring 34 and the inside of the adjustment ring 33 . Also, this movable ring 3
4 has a machine screw 38, which is fitted into a key groove 39 formed on the outer periphery of the cylinder 22, so that it cannot be rotated. Therefore, by rotating the adjustment ring 33, it can be moved up and down, and the tank 35 can be moved up and down. Volume can be adjusted.

Furthermore, a resistor 40 is provided between the tip of the cylinder 18 and the cylinder 23 to partition the flow of gas, and the blank cartridge 19 inside the cylinder 18 and the resistor 40
A gas reservoir chamber 41 is formed between them. This resistor 40
consists of a partition plate having an opening 42, and this opening 4
The cross-sectional area of cylinder 22 is smaller than that of cylinder 22.

The cell processing injection device 11 and the injection speed adjustment device 10 have the above-mentioned configurations and operate as follows.

First, preparations are made as follows. A sample 26 is placed on the stand 12. Cell processing material 2 in cylinder 22
The piston 23 to which 4 is attached is housed and attached to the cylindrical body 17 with the resistor 40 sandwiched therebetween. The movable ring 34 is screwed in, the machine screw 38 is stopped, and the adjustment ring 33 is rotated to set the volume of the tank 35 to an appropriate size. Blank cartridge 19
and fix the holder 16 at an appropriate height. Cover the case 13 and reduce the pressure inside the case 13.

Next, the ignition mechanism 20 is activated. As a result, high-pressure gas is generated from the gunpowder in the blank cartridge 19, and this gas fills the gas reservoir chamber 41 and tank 35 due to the flow resistance of the opening 42 of the resistor 40, and then flows into the cylinder 22. It moves forward, pushes the piston 23, accelerates at high speed, and collides with the injection part 27. Due to this collision, the cell treatment material 24 is injected through the small hole 28, hits the sample 26, and performs cell treatment such as injecting genetic material into the cells. At this time, the exhaust holes 29,..., 30,..., 31,
Air remaining in front of the piston 23 is exhausted from...

That is, the tank 35 receives gas and adjusts the pressure behind the piston 23 to adjust the speed of the piston 23, that is, the injection speed of the cell processing material 24, and the larger the volume, the faster the injection speed becomes. The speed will be low.

The resistor 40 provides resistance to the flow of gas to the piston 23, prevents the piston from moving at a low speed before the gas generating section 21 generates sufficient gas, and prevents the piston from moving at a low speed. This aims to increase the speed and expand the range of speed adjustment.

According to experiments, the maximum speed at collision of the piston is 660 m/s when no resistor is used, but when this resistor 51 (the cross-sectional area of the opening is made half the cross-sectional area of the cylinder) When using the following method, the speed was 1080 m/s.

Although the illustrated example has been described above, the present invention can have various other configurations. For example, the structure of the tank is not limited to the above example, and it is also possible to have a structure in which a part of the cylinder has a large diameter and this large diameter part can be expanded and contracted, in other words, it can accept gas and adjust its volume. It is optional as long as it is possible. The resistor is
Since it increases the injection speed and widens the range, it is possible to omit the low-speed device, and its configuration is not limited to the above example, but also includes a pin inserted from the outside, and a plate that breaks due to high pressure. It can have any configuration, such as a body. Furthermore, the positional relationship between the resistor and the outflow hole is not limited to providing the outflow hole upstream of the resistor as shown in the figure.
A downstream configuration is also possible.

Furthermore, the configuration of the injection device using the present invention is not limited to the above example. For example, the above example uses gunpowder as the gas generating section, but compressed air similar to an air gun is used. It is also possible to use a device in which the water is spouted out using a jetting valve. Furthermore, although the illustrated apparatus is designed to be used under reduced pressure conditions, it may also be used under normal pressure conditions.

[0025]

Effects of the Invention As described above, the injection speed adjusting device of the present invention uses a tank whose volume can be adjusted, receives gas from the gas generating section, adjusts its pressure, and adjusts the speed of the piston. As a result, the structure is simple, the speed is stable, and adjustment over a wide range can be easily realized. Furthermore, when used in conjunction with a resistor, the gas is once stored and reaches high pressure before pushing the piston, increasing the speed of the piston and expanding the range of speed adjustment.

[Brief explanation of the drawing]

FIG. 1 is a perspective view of an embodiment of the invention.

FIG. 2 is a vertical sectional view of the main part of the same example.

[Explanation of symbols]

10 Injection speed adjustment device 21 Gas generation part 22 Cylinder 23 Piston 24 Cell processing materials 25 Sample 27 Injection part 28 Small hole 35 Tank 40 Resistor 41 Gas reservoir chamber

Claims (2)

[Claims] 1. A cylinder that includes a gas generating section that instantaneously generates high-pressure gas, and a cylinder connected to the gas generating section that accommodates a piston having a cell processing material attached to its tip, and that moves the piston toward the distal end using gas. and an injection unit that has a small hole and is provided at the tip or an extension of the cylinder and causes a piston to collide with the cell processing material to inject the cell processing material from the small hole to the sample. An injection speed adjusting device for an injection device for cell processing, comprising a tank whose volume can be adjusted and which receives and stores a part of the gas flow into the cylinder, between the cylinder and the piston in the cylinder. 2. The injection device for cell processing according to claim 1, further comprising a gas reservoir chamber and a resistor that applies resistance to the flow of gas from the gas reservoir chamber to the piston, between the gas generating section and the piston. Injection speed adjustment device.