JPH0420140B2 - - Google Patents
Info
- Publication number
- JPH0420140B2 JPH0420140B2 JP58102599A JP10259983A JPH0420140B2 JP H0420140 B2 JPH0420140 B2 JP H0420140B2 JP 58102599 A JP58102599 A JP 58102599A JP 10259983 A JP10259983 A JP 10259983A JP H0420140 B2 JPH0420140 B2 JP H0420140B2
- Authority
- JP
- Japan
- Prior art keywords
- electrophoresis
- zone
- gel
- support
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001962 electrophoresis Methods 0.000 claims description 32
- 239000000243 solution Substances 0.000 claims description 11
- 150000007523 nucleic acids Chemical group 0.000 claims description 9
- 238000013508 migration Methods 0.000 claims description 8
- 230000005012 migration Effects 0.000 claims description 8
- 239000007853 buffer solution Substances 0.000 claims description 6
- 102000039446 nucleic acids Human genes 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 6
- 238000010828 elution Methods 0.000 claims description 5
- 239000012149 elution buffer Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 description 11
- 238000000034 method Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 230000002285 radioactive effect Effects 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- 238000007796 conventional method Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 238000000211 autoradiogram Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58102599A JPS59228156A (ja) | 1983-06-10 | 1983-06-10 | 核酸塩基配列自動決定装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58102599A JPS59228156A (ja) | 1983-06-10 | 1983-06-10 | 核酸塩基配列自動決定装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59228156A JPS59228156A (ja) | 1984-12-21 |
| JPH0420140B2 true JPH0420140B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1992-03-31 |
Family
ID=14331698
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58102599A Granted JPS59228156A (ja) | 1983-06-10 | 1983-06-10 | 核酸塩基配列自動決定装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59228156A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4729947A (en) * | 1984-03-29 | 1988-03-08 | The Board Of Regents Of The University Of Nebraska | DNA sequencing |
-
1983
- 1983-06-10 JP JP58102599A patent/JPS59228156A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS59228156A (ja) | 1984-12-21 |
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