JPH04200382A - Fermentation tank - Google Patents

Fermentation tank

Info

Publication number
JPH04200382A
JPH04200382A JP2334842A JP33484290A JPH04200382A JP H04200382 A JPH04200382 A JP H04200382A JP 2334842 A JP2334842 A JP 2334842A JP 33484290 A JP33484290 A JP 33484290A JP H04200382 A JPH04200382 A JP H04200382A
Authority
JP
Japan
Prior art keywords
raw material
fermenter
product
microorganisms
fermentation tank
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2334842A
Other languages
Japanese (ja)
Inventor
Toru Taniguchi
徹 谷口
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Reika Kogyo KK
Original Assignee
Reika Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reika Kogyo KK filed Critical Reika Kogyo KK
Priority to JP2334842A priority Critical patent/JPH04200382A/en
Publication of JPH04200382A publication Critical patent/JPH04200382A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16Particles; Beads; Granular material; Encapsulation
    • C12M25/18Fixed or packed bed
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/12Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Abstract

PURPOSE:To improve the profitability and production efficiency of a fermentation tank by installing a raw material-injecting means, a product-discharging means, a vibration-stirring means, etc., to the closed fermentation tank holding microorganisms carriers. CONSTITUTION:A raw material is injected into the main body 11 of a fermentation tank through an injection opening 12, controlled to a desired reaction condition and moved in the vertical direction with a vibration plate 15 through a motor 17 and an eccentric cam 18 to allow a solution solution comprising a mixture of the raw material and the product to flow. Carriers 10 are immobilized to the main body 11 with supporting members 11a, and microorganisms are brought into contact with the raw material without peeling from the carriers 10 for the good proceeding of the reaction. The reaction product is taken out through a product-discharging opening 13.

Description

【発明の詳細な説明】 [産業上の利用分野] 本発明は微生物により発酵が行われる発酵槽、特に自動
攪拌される密閉型発酵槽に関する。
DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a fermenter in which fermentation is carried out by microorganisms, and particularly to a closed fermenter with automatic stirring.

[従来の技術] 工業用及び生活用として大量に利用される物質、例えば
エタノールなどは、大きく分けて2つの方法で製造され
ている。すなわちエチレンを水和させてエタノールを得
る化学的方法と、炭水化物を原料としてそれを酵母など
により発酵させてエタノールを得る生物学的方法である
[Prior Art] Substances that are used in large quantities for industrial and daily use, such as ethanol, are generally produced by two methods. Specifically, there are two methods: a chemical method to obtain ethanol by hydrating ethylene, and a biological method to obtain ethanol by fermenting carbohydrates with yeast or the like.

大量にエタノールを得るためには工業的に化学的製法を
適用することが適しているか、近年の生物学的方法の進
歩により生物学的製法によっても化学的製法と同様に、
大量のエタノールを得ることができる。
Is it appropriate to apply industrial chemical production methods to obtain ethanol in large quantities? With the recent advances in biological methods, it is possible to use biological production methods in the same way as chemical production methods.
A large amount of ethanol can be obtained.

すなわち、原料に対する微生物の濃度を高めることによ
り反応速度を上げることができ、生成物の大量生産が図
れるようになったものである。具体的には、多孔性の物
質(いわゆる担体)に微生物を付着させ、その微生物が
付着した担体を集積させ、そこで原料を一定時間発酵さ
せた後、生成物を得ている。多孔性物質である担体は表
面積が大きいため、多くの微生物をその上に付着させる
ことができ、原料に対する微生物の密度が高くなるので
、発酵速度の向上が図れる。
In other words, by increasing the concentration of microorganisms in the raw materials, the reaction rate can be increased, making it possible to mass-produce products. Specifically, microorganisms are attached to a porous substance (a so-called carrier), the carrier with the attached microorganisms is accumulated, and the raw material is fermented there for a certain period of time, and then the product is obtained. Since the carrier, which is a porous material, has a large surface area, many microorganisms can be attached thereto, and the density of microorganisms relative to the raw material becomes high, so that the fermentation rate can be improved.

[発明が解決しようとする課題] しかしながら、大量生産を図るために発酵槽を大型化す
ると発酵槽内て不均衡か生じ、その結果、反応に偏りが
生じてしまい、効率か悪くなる。それを解決するために
、この発酵槽を攪拌することが考えられ、実際には、発
酵槽内のガスを用いて攪拌するガス攪拌と、発酵槽内の
液体をポンプで攪拌するポンプ攪拌が行われていた。し
かし、ポンプ攪拌では発酵槽内の不均衡が十分には解消
されず、一方、ガス攪拌では、これが強力すぎるために
、担体に付着し難い嫌気性菌では特に、担体に担持され
ている微生物が剥離してかえって効率の悪化を招く結果
となっていた。
[Problems to be Solved by the Invention] However, when the size of the fermenter is increased in order to achieve mass production, an imbalance occurs within the fermenter, resulting in biased reaction and poor efficiency. To solve this problem, it has been considered to stir the fermenter, and in reality, gas agitation using the gas in the fermenter and pump agitation using a pump to agitate the liquid in the fermenter are being carried out. I was worried. However, pump agitation does not sufficiently eliminate the imbalance within the fermenter, while gas agitation is too powerful, which can cause microorganisms carried on the carrier to become weaker, especially anaerobic bacteria that are difficult to adhere to the carrier. This resulted in peeling, which resulted in a deterioration of efficiency.

そこで本発明の目的は、担体に担持させている微生物を
剥離させることなく発酵槽内を攪拌することにより、発
酵の効率を高めることにある。
Therefore, an object of the present invention is to improve the efficiency of fermentation by stirring the inside of the fermenter without removing the microorganisms supported on the carrier.

[課題を解決するための手段] かかる目的を達成するために、本発明は、原料を注入す
る注入口と、生成物を取り出す取出し口とを有する密閉
型発酵槽においてその中にこの密閉型発酵槽内を振動に
より攪拌させる振動板を設置し、それにより密閉型発酵
槽内の原料を振動攪拌することを特徴とする。
[Means for Solving the Problems] In order to achieve the above object, the present invention provides a method for producing a closed fermenter in a closed fermenter having an inlet for injecting raw materials and an outlet for taking out a product. It is characterized by installing a diaphragm that stirs the inside of the tank by vibration, thereby vibrating and stirring the raw materials in the closed fermenter.

[作用] 以上のように構成された本発明にかかる発酵槽において
は、振動板の振動攪拌により密閉型発酵槽中の液体が動
くことにより、原料の濃度が均一化し、かつ微生物と原
料の接触が促進され、これにより、微生物による発酵が
進行する。
[Function] In the fermenter according to the present invention configured as described above, the liquid in the closed fermenter is moved by the vibration stirring of the diaphragm, so that the concentration of the raw material is made uniform and the contact between the microorganisms and the raw material is prevented. This promotes fermentation by microorganisms.

[実施例] 第1図は本発明に係る発酵槽の一実施例を表す断面図で
ある。
[Example] FIG. 1 is a sectional view showing an example of a fermenter according to the present invention.

この図において、微生物を担持させた担体10は、発酵
槽本体11内の支持部材11aによって固定化されてお
り、発酵槽本体11には、原料を注入する原料注入口1
2と生成物を取出す生成物取出口13とが設置されてい
る。
In this figure, a carrier 10 carrying microorganisms is immobilized by a support member 11a in a fermenter body 11, and a raw material injection port 1 for injecting raw materials is provided in the fermenter body 11.
2 and a product outlet 13 for taking out the product.

さらに、発酵槽本体11内には振動板15が設置されて
いて、実施例において、この振動板15を駆動するモー
タ17が外部に設置されている。
Furthermore, a diaphragm 15 is installed inside the fermenter main body 11, and in the embodiment, a motor 17 for driving this diaphragm 15 is installed outside.

このモータ17の回転により偏心カム18か回転し、振
動板15を上下運動させる。一方、発酵槽本体11の底
部において、振動板15か挿入されている箇所は、ダイ
ヤフラム21により、振動板15が動いても中身が漏れ
ないように構成されている。
The rotation of the motor 17 causes the eccentric cam 18 to rotate, causing the diaphragm 15 to move up and down. On the other hand, at the bottom of the fermenter main body 11, the portion where the diaphragm 15 is inserted is configured with a diaphragm 21 to prevent the contents from leaking even if the diaphragm 15 moves.

まず、原料が注入口12より注入され、発酵槽本体11
内で発酵される際、反応条件が設定された後、振動板1
5の上下運動が行われる。この時、微生物を担持させた
担体10は、発酵槽本体11内の支持部材11aによっ
て固定化されているために動くことができず、一方、原
料と生成物の混合物である発酵槽本体11内の液体部分
14は、振動板15の作用を受けて発酵槽内を流動する
First, raw materials are injected from the injection port 12, and the fermenter body 11
After the reaction conditions are set, the diaphragm 1
5 up and down movements are performed. At this time, the carrier 10 carrying microorganisms cannot move because it is immobilized by the support member 11a in the fermenter body 11, while the carrier 10 carrying the microorganisms cannot move. The liquid portion 14 flows in the fermenter under the action of the diaphragm 15.

これにより、液体部分14の原料の組成が均一にされ、
更に、微生物と原料の接触を良好にすることができる。
This makes the composition of the raw material in the liquid portion 14 uniform,
Furthermore, good contact between microorganisms and raw materials can be achieved.

ここで、この振動板15の作用は上下運動であるために
、ガス攪拌のような激しさはなく、微生物が担体から剥
離することかない。
Here, since the action of the diaphragm 15 is vertical movement, it is not as violent as gas agitation, and the microorganisms are not separated from the carrier.

それに加えて、液体部分14の原料の組成を均一にし、
かつ微生物と原料の接触を良好にすることができるため
、生成物を得るまでの時間を短縮することができる。
In addition, the composition of the raw material in the liquid portion 14 is made uniform;
In addition, since the contact between the microorganism and the raw material can be improved, the time required to obtain the product can be shortened.

反応終了後、生成物は生成物取出口13から取り出され
、それと共に原料注入口12から新な原料が注入される
。その後は、同様にして製品化か繰り返される。したが
って、反応条件を適当に設置すれば、原料の注入と同時
に生成物の取出しを行うことができ、連続した反応シス
テムを形成させることが可能となる。
After the reaction is completed, the product is taken out from the product outlet 13, and at the same time, a new raw material is injected from the raw material inlet 12. After that, the same process is repeated to commercialize the product. Therefore, if the reaction conditions are set appropriately, the product can be taken out at the same time as the raw materials are injected, making it possible to form a continuous reaction system.

また、第2図に示されるように発酵槽本体11の底部に
原料注入口12を設け、この原料注入口12の途中に振
動板15を設け、原料と微生物の担持された担体を混合
する手段を用いても良い。
Further, as shown in FIG. 2, a raw material inlet 12 is provided at the bottom of the fermenter main body 11, and a diaphragm 15 is provided in the middle of this raw material inlet 12, as a means for mixing the raw material and the carrier carrying microorganisms. You may also use

更に、振動板15を振動させる手段は、モータと偏心カ
ムの組合せによるものに限られず、上下運動をさせる機
構の手段であれば差し支えない。
Further, the means for vibrating the diaphragm 15 is not limited to a combination of a motor and an eccentric cam, and any mechanism for vertical movement may be used.

なお、本発明において、微生物とは微生物と同様の触媒
作用を有する酵素をも含む。
In the present invention, microorganisms also include enzymes that have the same catalytic action as microorganisms.

[発明の効果] 以上のように構成された本発明によれば、発酵による効
率が上るために、生物学的製法による製造効率が向上し
、製品製造のコストの低下などが図れる。
[Effects of the Invention] According to the present invention configured as described above, since the efficiency of fermentation is increased, the production efficiency of the biological production method is improved, and the cost of product production can be reduced.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図は、本発明の第一実施例にかかる発酵槽の断面図
、 第2図は、本発明の第二実施例にかかる発酵槽の断面図
である。 10 ・・・担体 11 ・・・発酵槽本体 12 ・・・原料注入口 13 ・・・生成物取出口 15 ・・・振動板 17 ・・・モータ 18 ・・・偏心カム 21 ・・・ダイヤフラム ]d 第1図
FIG. 1 is a cross-sectional view of a fermenter according to a first embodiment of the present invention, and FIG. 2 is a cross-sectional view of a fermenter according to a second embodiment of the present invention. 10 ... carrier 11 ... fermenter body 12 ... raw material inlet 13 ... product outlet 15 ... diaphragm 17 ... motor 18 ... eccentric cam 21 ... diaphragm] d Figure 1

Claims (1)

【特許請求の範囲】 微生物が担持された担体を保持する密閉型発酵槽と、 微生物により発酵される原料を前記密閉型発酵槽内に注
入する注入手段と、 前記密閉型発酵槽において生成した生成物を取出す取出
手段と、 前記密閉型発酵槽内に設置され、該密閉型発酵槽内に振
動を付与する振動板と、 前記振動板を駆動させる手段と、 から構成され、 密閉型発酵槽内の原料を振動攪拌することを特徴とする
発酵槽。
[Scope of Claims] A closed fermenter that holds a carrier carrying microorganisms, an injection means for injecting a raw material to be fermented by the microorganism into the closed fermenter, and a product produced in the closed fermenter. It is comprised of: a take-out means for taking out an object; a diaphragm installed in the closed fermenter to apply vibration to the inside of the closed fermenter; and a means for driving the diaphragm, A fermenter characterized by vibratory stirring of raw materials.
JP2334842A 1990-11-29 1990-11-29 Fermentation tank Pending JPH04200382A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2334842A JPH04200382A (en) 1990-11-29 1990-11-29 Fermentation tank

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2334842A JPH04200382A (en) 1990-11-29 1990-11-29 Fermentation tank

Publications (1)

Publication Number Publication Date
JPH04200382A true JPH04200382A (en) 1992-07-21

Family

ID=18281830

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2334842A Pending JPH04200382A (en) 1990-11-29 1990-11-29 Fermentation tank

Country Status (1)

Country Link
JP (1) JPH04200382A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006180782A (en) * 2004-12-27 2006-07-13 Hiroshima Univ Method for producing hydrogen and ethanol from biodiesel waste liquid and kit for performing the method
WO2014175723A3 (en) * 2013-04-24 2014-12-24 Universiti Kebangsaan Malaysia A bioreactor
JP2019071842A (en) * 2017-10-17 2019-05-16 澁谷工業株式会社 Apparatus for producing cell aggregate structure

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006180782A (en) * 2004-12-27 2006-07-13 Hiroshima Univ Method for producing hydrogen and ethanol from biodiesel waste liquid and kit for performing the method
JP4665157B2 (en) * 2004-12-27 2011-04-06 国立大学法人広島大学 Process for producing hydrogen and ethanol from biodiesel wastewater
WO2014175723A3 (en) * 2013-04-24 2014-12-24 Universiti Kebangsaan Malaysia A bioreactor
JP2019071842A (en) * 2017-10-17 2019-05-16 澁谷工業株式会社 Apparatus for producing cell aggregate structure

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